pycom16g12720

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012734
Physical Location & Seq
Forward (+)
8991837 .. 8992376
540 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g12720.1

Sequence Viewer

Length: 540 bp
ATGATATTTGATTCTGAGTCGGGCAAATGGAGGAAGTTTGTTTCGTTTCAGGATGATCAGTTCACCCATATGAATAAGAGCCAAGTCGTGTTTGTTAATGGTGCACTTCATTGGTTGACTGGTAGTTCCTGTATTCTCGCGCTTGATTTGGAGTTTGATGTTTGGAGGAAGATGTCATTGCCGGAACAAGTGACTTGTGTGTCCGGAAATAGGTTTTACTTGTTGGAGTCAGATGGTTGCCTGTCGGTGATCCAGATTTCAGATGCTTGGATGAAAATTTGGGTGTTGAAAGGTTACGGGAGAGAGGAGTGGCATTTGGTGGATACGGTGAGTCTTCGATGTATCAAAGGACTTGTGCCGGGCATCTTCCCCATATGTCAGACTGGTGAATGCGTTTTCTTGGCAACCCACAAGCAGATACTGGTATTTTATCGCAAGACTCGTGTGTGGAAAGAGATGTACTCTGTGAAGAACAGCTCTACACTCCCGTTGTGGTACTCGGCACATGCATTTCGGAGCACGATTTTGTCTTGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

180

Amino Acids

20.72

Weight (kDa)

8.47

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 2 - 157 5.1e-08 F-box associated beta propeller domain
Beta-prop_AT5G49610-like PF23635 7 - 114 1.1e-07 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012734)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49610
fragaria_vesca FvH4_4g20690
malus_domestica MD13G1148700.v1.1 MD16G1149000.v1.1
prunus_persica Prupe.1G112700_v2.0.a1
pyrus_communis pycom13g12800 pycom16g12720
rosa_chinensis RchiOBHm_Chr4g0426111
rosa_laevigata RLG00000007318
rosa_multiflora Rmu_co8375493.1_g000001 Rmu_ssc0000281.1_g000004
rosa_roxburghii Rroxscaffold_5G00368100
rosa_rugosa Rorug04G0209100
rosa_samantha Rh4AG265600 Rh4BG271700 Rh4CG284600 Rh4DG267800
rosa_wichuraiana Rw4G022950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 199
AccII CGCG 1 cut(s) 140
AccIII TCCGGA 1 cut(s) 203
AclWI GGATC 1 cut(s) 244
AcsI RAATTY 1 cut(s) 276
AfaI GTAC 2 cut(s) 461, 497
AfiI CCNNNNNNNGG 1 cut(s) 210
AgsI TTSAA 1 cut(s) 289
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 1 cut(s) 477
AluI AGCT 1 cut(s) 477
Alw21I GWGCWC 2 cut(s) 106, 521
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 1 cut(s) 244
AlwNI CAGNNNCTG 1 cut(s) 421
Aor13HI TCCGGA 1 cut(s) 203
ApaLI GTGCAC 1 cut(s) 102
ApoI RAATTY 1 cut(s) 276
AspLEI GCGC 1 cut(s) 142
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 4 cut(s) 55, 259, 340, 398
BaeGI GKGCMC 1 cut(s) 106
BauI CACGAG 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 2 cut(s) 106, 521
BccI CCATC 1 cut(s) 227
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 1 cut(s) 360
BfuI GTATCC 1 cut(s) 316
Bme1390I CCNGG 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 360
BmsI GCATC 2 cut(s) 253, 372
BpiI GAAGAC 1 cut(s) 326
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpuMI CCSGG 1 cut(s) 360
BsaWI WCCGGW 1 cut(s) 203
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse1I ACTGG 3 cut(s) 124, 388, 426
Bse3DI GCAATG 1 cut(s) 176
BseAI TCCGGA 1 cut(s) 203
BseGI GGATG 2 cut(s) 58, 276
BseLI CCNNNNNNNGG 1 cut(s) 210
BseMI GCAATG 1 cut(s) 176
BseMII CTCAG 1 cut(s) 6
BseNI ACTGG 3 cut(s) 124, 388, 426
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 140
BsiHKAI GWGCWC 2 cut(s) 106, 521
BsiSI CCGG 3 cut(s) 182, 204, 359
BslI CCNNNNNNNGG 1 cut(s) 210
BsmI GAATGC 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 106, 521
Bsp13I TCCGGA 1 cut(s) 203
Bsp143I GATC 2 cut(s) 55, 249
BspCNI CTCAG 1 cut(s) 7
BspEI TCCGGA 1 cut(s) 203
BspFNI CGCG 1 cut(s) 140
BspPI GGATC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 176
BsrI ACTGG 3 cut(s) 124, 388, 426
BssMI GATC 2 cut(s) 55, 249
BssSI CACGAG 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 441
Bst4CI ACNGT 1 cut(s) 328
BstDEI CTNAG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 58, 276
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 2 cut(s) 58, 252
BstMBI GATC 2 cut(s) 55, 249
BstNSI RCATGY 1 cut(s) 509
BstSCI CCNGG 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 106
BstUI CGCG 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 326
BsuI GTATCC 1 cut(s) 316
BtsCI GGATG 2 cut(s) 58, 276
CaiI CAGNNNCTG 1 cut(s) 421
CfoI GCGC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 460, 496
CviAII CATG 1 cut(s) 506
CviJI RGCY 2 cut(s) 81, 477
CviKI_1 RGCY 2 cut(s) 81, 477
CviQI GTAC 2 cut(s) 460, 496
DdeI CTNAG 1 cut(s) 15
DpnI GATC 2 cut(s) 57, 251
DpnII GATC 2 cut(s) 55, 249
DrdI GACNNNNNNGTC 1 cut(s) 199
DseDI GACNNNNNNGTC 1 cut(s) 199
EcoT22I ATGCAT 1 cut(s) 511
FaeI CATG 1 cut(s) 509
FaiI YATR 5 cut(s) 69, 71, 374, 376, 507
FatI CATG 1 cut(s) 505
FauNDI CATATG 2 cut(s) 69, 374
FbaI TGATCA 1 cut(s) 55
FokI GGATG 2 cut(s) 65, 283
GlaI GCGC 1 cut(s) 141
HapII CCGG 3 cut(s) 182, 204, 359
HhaI GCGC 1 cut(s) 142
Hin1II CATG 1 cut(s) 509
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HinfI GANTC 5 cut(s) 11, 17, 227, 331, 439
HpaII CCGG 3 cut(s) 182, 204, 359
HphI GGTGA 4 cut(s) 55, 259, 340, 398
Hpy166II GTNNAC 3 cut(s) 63, 104, 117
Hpy188I TCNGA 5 cut(s) 16, 232, 262, 381, 516
Hpy188III TCNNGA 3 cut(s) 50, 204, 253
Hpy8I GTNNAC 3 cut(s) 63, 104, 117
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4V TGCA 2 cut(s) 104, 509
HpyF3I CTNAG 1 cut(s) 15
Hsp92II CATG 1 cut(s) 509
HspAI GCGC 1 cut(s) 140
Kpn2I TCCGGA 1 cut(s) 203
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 2 cut(s) 55, 249
LmnI GCTCC 1 cut(s) 516
LweI GCATC 2 cut(s) 253, 372
MaeIII GTNAC 2 cut(s) 190, 293
MalI GATC 2 cut(s) 57, 251
MboI GATC 2 cut(s) 55, 249
MboII GAAGA 4 cut(s) 181, 326, 358, 481
MhlI GDGCHC 2 cut(s) 106, 521
MluCI AATT 1 cut(s) 276
MlyI GAGTC 4 cut(s) 26, 236, 340, 433
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 3 cut(s) 24, 159, 298
Mph1103I ATGCAT 1 cut(s) 511
MroI TCCGGA 1 cut(s) 203
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 3 cut(s) 182, 204, 359
MspR9I CCNGG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 395
MvnI CGCG 1 cut(s) 140
NciI CCSGG 1 cut(s) 360
NdeI CATATG 2 cut(s) 69, 374
NdeII GATC 2 cut(s) 55, 249
NlaIII CATG 1 cut(s) 509
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 1 cut(s) 509
PcsI WCGNNNNNNNCGW 1 cut(s) 439
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 11
PleI GAGTC 4 cut(s) 25, 235, 339, 433
PpsI GAGTC 4 cut(s) 25, 235, 339, 433
PstNI CAGNNNCTG 1 cut(s) 421
RsaI GTAC 2 cut(s) 461, 497
RsaNI GTAC 2 cut(s) 460, 496
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 1 cut(s) 96
Sau3AI GATC 2 cut(s) 55, 249
SchI GAGTC 4 cut(s) 26, 236, 340, 433
ScrFI CCNGG 1 cut(s) 360
SduI GDGCHC 2 cut(s) 106, 521
SetI ASST 3 cut(s) 215, 295, 479
SfaNI GCATC 2 cut(s) 253, 372
SmiMI CAYNNNNRTG 1 cut(s) 68
Sse9I AATT 1 cut(s) 276
StyD4I CCNGG 1 cut(s) 358
TaaI ACNGT 1 cut(s) 328
TaqI TCGA 1 cut(s) 337
TasI AATT 1 cut(s) 276
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 1 cut(s) 11
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 86, 98, 287
VneI GTGCAC 1 cut(s) 102
XapI RAATTY 1 cut(s) 276
XceI RCATGY 1 cut(s) 509
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.