MD13G1148700.v1.1

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012734
Physical Location & Seq
Forward (+)
11702440 .. 11703519
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1148700.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGATTCCCAAAGACGTGTTTTTTTCCCGGACGAAGTCGTCCTCCAAATCCTCTCAAAACTGCCCATTAAGTCTCTTTTCAGGAGCAAAACTGTTTGTAAACTTTGGTACAGATTGGTTTCTGATGAGTATTTCATTCAATTGTACAATGAAACTTCTGCCAAGAACCCTATGCTATTGGTTGAGGTCTCCGACTCGTTAGGATCGAAATCTGATTTGATTGTTGTGGACAATCGGAGGGGTGTTTCCGAATTTTCGTTAGATTTCTTGAGGGATAGGGTTAAGGTCAGAGCCTCCTGTAATGGCTTGTTGTGTTGCTCTAGCATTCCTGATAAGGATGTTTACTATCTCTGTAATCCCATGACTCGGGAGTTCAAGTTGCTTCCGAAGAGTAGGGAGCGGCATGTCACCCGATTTTATCCTGATGGTGAGGCAACCCTGGTGGGATTAGCTTGCAATTTGTCTACTCAGAAGTTTAATGTTGTTTTAGCGGGTTATCATCGTACTTTTGGTCATAGGTCAGATGGAACTTTTGTATGTATGATTTTTGATTCTGAGTCGAACAAATGGAGGAAGTTTGTTTCGTTTCACGGTGATCAATTCACCCATATGAATAAGAACCAAGTCGTGTTTGTTAATGGTGCACTACATTGGTTGACTGGTAGTTCCTGTATTCTCACGCTCAATTTGGAGTATGATGTTTGGAGGAAGATGTCATTGCCGGAACAAGTGACTTGTGTGTCTGGAAATAGGTTTTACTTGTTGGAGTCGGATGGTTGCCTGTCGGTGATCCAGATTTCAGATGCTTGGATGAAAACTTGGGTGTTGAAAGAGTACGAGAGTGAGGAATGGCATTTGGTGGATACGGTGAGTCTTCGATGTATCAAAGGACTTGTCCCAGGCATCTTCCCCATATGTCAGACTGGTGAATGTGTTTTCTTGGCAACCCACAAGCAGATATTGGTGTTTTATCGGAAGACTCGTGTGTGGAAAGAGATGTACTCTGTGAAGAACAGCTCCACACTCCCATTGTGGTACTCGGCACATGCATTTCGGGGCACAATTTTTTCTTGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

360

Amino Acids

41.52

Weight (kDa)

8.76

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 10 - 44 1.6e-09 F-box-like
F-box PF00646 10 - 45 5e-09 F-box domain
FBA_3 PF08268 83 - 324 4.8e-10 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 92 - 288 7.4e-08 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 94 - 300 8.1e-09 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 98 - 336 2e-15 F-box associated beta propeller domain
Beta-prop_AT5G49610-like PF23635 114 - 294 4.2e-06 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012734)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49610
fragaria_vesca FvH4_4g20690
malus_domestica MD13G1148700.v1.1 MD16G1149000.v1.1
prunus_persica Prupe.1G112700_v2.0.a1
pyrus_communis pycom13g12800 pycom16g12720
rosa_chinensis RchiOBHm_Chr4g0426111
rosa_laevigata RLG00000007318
rosa_multiflora Rmu_co8375493.1_g000001 Rmu_ssc0000281.1_g000004
rosa_roxburghii Rroxscaffold_5G00368100
rosa_rugosa Rorug04G0209100
rosa_samantha Rh4AG265600 Rh4BG271700 Rh4CG284600 Rh4DG267800
rosa_wichuraiana Rw4G022950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 38, 739
AccBSI CCGCTC 1 cut(s) 400
AccI GTMKAC 1 cut(s) 464
AciI CCGC 2 cut(s) 400, 491
AclWI GGATC 2 cut(s) 211, 784
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 6 cut(s) 110, 146, 505, 836, 1001, 1037
AfiI CCNNNNNNNGG 1 cut(s) 366
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 3 cut(s) 140, 376, 829
AjiI CACGTC 1 cut(s) 17
AjnI CCWGG 2 cut(s) 438, 898
AluBI AGCT 2 cut(s) 452, 1017
AluI AGCT 2 cut(s) 452, 1017
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 2 cut(s) 78, 193
Alw44I GTGCAC 1 cut(s) 642
AlwI GGATC 2 cut(s) 211, 784
Ama87I CYCGRG 1 cut(s) 366
ApaLI GTGCAC 1 cut(s) 642
ApoI RAATTY 1 cut(s) 251
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 7 cut(s) 400, 440, 595, 605, 799, 880, 938
AvaI CYCGRG 1 cut(s) 366
BaeGI GKGCMC 2 cut(s) 646, 1061
BauI CACGAG 1 cut(s) 981
BbsI GAAGAC 2 cut(s) 866, 983
Bbv12I GWGCWC 1 cut(s) 646
BccI CCATC 3 cut(s) 419, 518, 767
BciT130I CCWGG 2 cut(s) 440, 900
BciVI GTATCC 1 cut(s) 856
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 1 cut(s) 29
BcoDI GTCTC 2 cut(s) 78, 193
BfaI CTAG 1 cut(s) 321
BfuI GTATCC 1 cut(s) 856
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
Bme1390I CCNGG 3 cut(s) 29, 440, 900
BmeT110I CYCGRG 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 17
BmrFI CCNGG 3 cut(s) 29, 440, 900
BmsI GCATC 2 cut(s) 793, 912
BpiI GAAGAC 2 cut(s) 866, 983
BplI GAGNNNNNCTC 2 cut(s) 986, 1018
BpuEI CTTGAG 1 cut(s) 289
BpuMI CCSGG 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 208
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 2 cut(s) 438, 898
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 664, 928
Bse3DI GCAATG 1 cut(s) 716
Bse8I GATNNNNATC 1 cut(s) 208
BseBI CCWGG 2 cut(s) 440, 900
BseDI CCNNGG 2 cut(s) 438, 898
BseGI GGATG 3 cut(s) 343, 778, 816
BseJI GATNNNNATC 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 2 cut(s) 482, 546
BseNI ACTGG 2 cut(s) 664, 928
BseSI GKGCMC 2 cut(s) 646, 1061
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 2 cut(s) 29, 722
BslFI GGGAC 1 cut(s) 881
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 2 cut(s) 78, 193
BsmFI GGGAC 1 cut(s) 881
BsmI GAATGC 1 cut(s) 324
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 646, 1061
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 3 cut(s) 203, 595, 789
BspACI CCGC 2 cut(s) 400, 491
BspCNI CTCAG 2 cut(s) 481, 547
BspPI GGATC 2 cut(s) 211, 784
BspTNI GGTCTC 1 cut(s) 193
BsrBI CCGCTC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 716
BsrGI TGTACA 1 cut(s) 144
BsrI ACTGG 2 cut(s) 664, 928
BssECI CCNNGG 2 cut(s) 438, 898
BssMI GATC 3 cut(s) 203, 595, 789
BssSI CACGAG 1 cut(s) 981
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 2 cut(s) 440, 900
Bst4CI ACNGT 3 cut(s) 94, 593, 868
Bst6I CTCTTC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 454
BstDEI CTNAG 2 cut(s) 468, 555
BstF5I GGATG 3 cut(s) 343, 778, 816
BstKTI GATC 3 cut(s) 206, 598, 792
BstMAI GTCTC 2 cut(s) 78, 193
BstMBI GATC 3 cut(s) 203, 595, 789
BstNI CCWGG 2 cut(s) 440, 900
BstNSI RCATGY 2 cut(s) 407, 1049
BstSCI CCNGG 3 cut(s) 27, 438, 898
BstSLI GKGCMC 2 cut(s) 646, 1061
BstV2I GAAGAC 2 cut(s) 866, 983
BsuI GTATCC 1 cut(s) 856
BtrI CACGTC 1 cut(s) 17
BtsCI GGATG 3 cut(s) 343, 778, 816
Cac8I GCNNGC 1 cut(s) 454
Csp6I GTAC 6 cut(s) 109, 145, 504, 835, 1000, 1036
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 3 cut(s) 361, 404, 1046
CviJI RGCY 4 cut(s) 293, 306, 452, 1017
CviKI_1 RGCY 4 cut(s) 293, 306, 452, 1017
CviQI GTAC 6 cut(s) 109, 145, 504, 835, 1000, 1036
DdeI CTNAG 2 cut(s) 468, 555
DpnI GATC 3 cut(s) 205, 597, 791
DpnII GATC 3 cut(s) 203, 595, 789
DrdI GACNNNNNNGTC 2 cut(s) 38, 739
DseDI GACNNNNNNGTC 2 cut(s) 38, 739
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 366
EcoRII CCWGG 2 cut(s) 438, 898
EcoT22I ATGCAT 1 cut(s) 1051
FaeI CATG 3 cut(s) 364, 407, 1049
FaqI GGGAC 1 cut(s) 881
FatI CATG 3 cut(s) 360, 403, 1045
FauI CCCGC 1 cut(s) 484
FauNDI CATATG 2 cut(s) 609, 914
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 464
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 3 cut(s) 350, 785, 823
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 1 cut(s) 321
GluI GCNGC 1 cut(s) 401
HapII CCGG 2 cut(s) 29, 722
Hin1II CATG 3 cut(s) 364, 407, 1049
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HinfI GANTC 8 cut(s) 5, 194, 364, 551, 557, 767, 871, 979
HpaII CCGG 2 cut(s) 29, 722
HphI GGTGA 7 cut(s) 400, 440, 595, 605, 799, 880, 938
Hpy166II GTNNAC 6 cut(s) 101, 229, 343, 465, 644, 657
Hpy188III TCNNGA 7 cut(s) 82, 268, 329, 368, 422, 744, 793
Hpy8I GTNNAC 6 cut(s) 101, 229, 343, 465, 644, 657
HpyCH4III ACNGT 3 cut(s) 94, 593, 868
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 3 cut(s) 456, 644, 1049
HpyF3I CTNAG 2 cut(s) 468, 555
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 3 cut(s) 364, 407, 1049
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 3 cut(s) 203, 595, 789
LmnI GCTCC 3 cut(s) 84, 397, 1022
LweI GCATC 2 cut(s) 793, 912
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 2 cut(s) 406, 730
MalI GATC 3 cut(s) 205, 597, 791
MbiI CCGCTC 1 cut(s) 400
MboI GATC 3 cut(s) 203, 595, 789
MboII GAAGA 6 cut(s) 400, 721, 866, 898, 988, 1021
MfeI CAATTG 1 cut(s) 140
MhlI GDGCHC 2 cut(s) 646, 1061
MluCI AATT 6 cut(s) 140, 251, 457, 599, 685, 1062
MlyI GAGTC 6 cut(s) 188, 358, 566, 776, 880, 973
MmeI TCCRAC 3 cut(s) 216, 744, 750
Mph1103I ATGCAT 1 cut(s) 1051
MseI TTAA 4 cut(s) 69, 282, 477, 636
MslI CAYNNNNRTG 1 cut(s) 608
MspI CCGG 2 cut(s) 29, 722
MspR9I CCNGG 3 cut(s) 29, 440, 900
MunI CAATTG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 2 cut(s) 440, 900
NciI CCSGG 1 cut(s) 29
NdeI CATATG 2 cut(s) 609, 914
NdeII GATC 3 cut(s) 203, 595, 789
NlaIII CATG 3 cut(s) 364, 407, 1049
NmeAIII GCCGAG 1 cut(s) 1019
NmuCI GTSAC 2 cut(s) 406, 730
NsiI ATGCAT 1 cut(s) 1051
NspI RCATGY 2 cut(s) 407, 1049
PcsI WCGNNNNNNNCGW 2 cut(s) 203, 979
PctI GAATGC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 5, 551
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 1 cut(s) 402
PleI GAGTC 6 cut(s) 188, 358, 565, 775, 879, 973
PpsI GAGTC 6 cut(s) 188, 358, 565, 775, 879, 973
Psp6I CCWGG 2 cut(s) 438, 898
PspGI CCWGG 2 cut(s) 438, 898
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 6 cut(s) 110, 146, 505, 836, 1001, 1037
RsaNI GTAC 6 cut(s) 109, 145, 504, 835, 1000, 1036
RseI CAYNNNNRTG 1 cut(s) 608
SaqAI TTAA 4 cut(s) 69, 282, 477, 636
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 3 cut(s) 203, 595, 789
SchI GAGTC 6 cut(s) 188, 358, 566, 776, 880, 973
ScrFI CCNGG 3 cut(s) 29, 440, 900
SduI GDGCHC 2 cut(s) 646, 1061
SetI ASST 7 cut(s) 19, 189, 288, 454, 521, 755, 1019
SfaNI GCATC 2 cut(s) 793, 912
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 6 cut(s) 140, 251, 457, 599, 685, 1062
SsiI CCGC 2 cut(s) 400, 491
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 3 cut(s) 27, 438, 898
TaaI ACNGT 3 cut(s) 94, 593, 868
TaiI ACGT 1 cut(s) 19
TaqI TCGA 3 cut(s) 206, 560, 877
TasI AATT 6 cut(s) 140, 251, 457, 599, 685, 1062
TatI WGTACW 2 cut(s) 144, 999
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 2 cut(s) 5, 551
Tru1I TTAA 4 cut(s) 69, 282, 477, 636
Tru9I TTAA 4 cut(s) 69, 282, 477, 636
TseFI GTSAC 2 cut(s) 406, 730
Tsp45I GTSAC 2 cut(s) 406, 730
TspDTI ATGAA 4 cut(s) 124, 165, 626, 827
Tth111I GACNNNGTC 1 cut(s) 35
VneI GTGCAC 1 cut(s) 642
XapI RAATTY 1 cut(s) 251
XceI RCATGY 2 cut(s) 407, 1049
XmiI GTMKAC 1 cut(s) 464
XspI CTAG 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.