Rw4G022950

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012734
Physical Location & Seq
Reverse (-)
48448746 .. 48450458
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022950.1

Sequence Viewer

Length: 1086 bp
ATGAATTCGCCAAGACGTGGCTTTTTCCCGGACGAAGTCGTCCTTCAAATTCTTTCGAGGCTGCCGATTAAGTCACTTTTCAGGAGCAAAACTGTGTGTAAACTTTGGTATAGATTGGTTTCTGATAAGTATTTCATTCAGCTGTACAATGAATCGGCTGTGAAGAACTCTATGTTATTGGTTGAGGTCTCTGACTCGTCTGGGTTGAAATCGAGTTTGATTTGTGTTGATGATCAGAAGGGTGTTTGTGAATTTGACTTGGATTTCTTGAAGGACAGGGTTAAGGTCAGGGCATCGTGTAATGGTTTGTTGTGCTGCTCTAGTATACCTGATAAGGGTGTTTACTATGTGTGTAACCCTATGACTCGGGAGTTTAAGCTGCTTCCCAAGAGTAGGGAGAGGAATGTGACTCGGTTTTACCCTGATGGTGAGGCCACTTTAGTCGGATTGGCTTGCAATTTGGCTTCTCAGAAGTTTAATGTGGTTTTGGCCGGCTATCACCGCACTTTTGGTCATAGGCCGGATGGGACTTTTAAGTGTATGATATATGATTCTGAGACGAACAAATGGAGGAAGTTTATTACTTTCCAGGATGATCAGTTTAGCCATATGAATAAGAACCAGGTTGTGTTTGTGAATGGTGCGCTGCATTGGTTGACGGGTGGTGCTTGTTCTTGTATACTTGTGCTTGATTTGGAGTACGATATGTGGAGGAAGATGTCGTTGCCTGATGAATTGAACTGTGGAATGGGGAATCGGTTTTACTTGTTGGAGTCGGATGGATGCTTGTCTGTGATACAGATTTCGGATACTTGGATGAAGATTTGGGTGTTGAAAGATTATGTGAGAGAGGAGTGGCGTTTGGTTGATACTGTCAGTCTGAGATGTATTAAAGGACTTGTTCCAGGGATTTTCCCCATATGTCAGACTGGTGAATATGTTCTGTTAGCAACACATAAGCAGATATTGGTGTTTCATCGCAGAAGTCGTGTGTGGAAAGAGATGTACTCTGTGAAGAACAGCTCTACACTCCCATTGTGGTACTCAGCTCATGCATTTCGGAGCACGATTTCTTCTTGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

361

Amino Acids

41.68

Weight (kDa)

8.91

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 45 2.5e-09 F-box domain
F-box-like PF12937 10 - 43 2.5e-09 F-box-like
FBA_1 PF07734 79 - 330 9.7e-16 F-box associated beta propeller domain
FBA_3 PF08268 82 - 316 3.6e-12 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 94 - 302 4e-13 F-box protein At3g26010-like, beta-propeller
Beta-prop_AT5G49610-like PF23635 96 - 341 3.8e-09 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012734)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49610
fragaria_vesca FvH4_4g20690
malus_domestica MD13G1148700.v1.1 MD16G1149000.v1.1
prunus_persica Prupe.1G112700_v2.0.a1
pyrus_communis pycom13g12800 pycom16g12720
rosa_chinensis RchiOBHm_Chr4g0426111
rosa_laevigata RLG00000007318
rosa_multiflora Rmu_co8375493.1_g000001 Rmu_ssc0000281.1_g000004
rosa_roxburghii Rroxscaffold_5G00368100
rosa_rugosa Rorug04G0209100
rosa_samantha Rh4AG265600 Rh4BG271700 Rh4CG284600 Rh4DG267800
rosa_wichuraiana Rw4G022950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccB7I CCANNNNNTGG 1 cut(s) 17
AccI GTMKAC 2 cut(s) 325, 679
AciI CCGC 1 cut(s) 502
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 3 cut(s) 4, 48, 251
AfaI GTAC 4 cut(s) 146, 701, 1007, 1043
AfiI CCNNNNNNNGG 3 cut(s) 17, 335, 393
AgsI TTSAA 5 cut(s) 47, 208, 271, 739, 835
AjiI CACGTC 1 cut(s) 17
AjnI CCWGG 3 cut(s) 588, 621, 904
AluBI AGCT 4 cut(s) 142, 379, 1023, 1049
AluI AGCT 4 cut(s) 142, 379, 1023, 1049
Alw21I GWGCWC 1 cut(s) 1067
Alw26I GTCTC 2 cut(s) 193, 551
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 3 cut(s) 432, 489, 518
ApeKI GCWGC 4 cut(s) 61, 315, 379, 646
ApoI RAATTY 3 cut(s) 4, 48, 251
Asp700I GAANNNNTTC 1 cut(s) 939
AspLEI GCGC 1 cut(s) 646
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 3 cut(s) 440, 491, 944
AvaI CYCGRG 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 1067
BbvI GCAGC 4 cut(s) 48, 302, 366, 633
BccI CCATC 3 cut(s) 419, 518, 773
BciT130I CCWGG 3 cut(s) 590, 623, 906
BciVI GTATCC 1 cut(s) 802
BclI TGATCA 2 cut(s) 232, 595
BcnI CCSGG 1 cut(s) 29
BcoDI GTCTC 2 cut(s) 193, 551
BfaI CTAG 1 cut(s) 321
BfuI GTATCC 1 cut(s) 802
BisI GCNGC 4 cut(s) 62, 316, 380, 647
BlsI GCNGC 4 cut(s) 63, 317, 381, 648
Bme1390I CCNGG 4 cut(s) 29, 590, 623, 906
BmeT110I CYCGRG 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 17
BmrFI CCNGG 4 cut(s) 29, 590, 623, 906
BmsI GCATC 2 cut(s) 302, 773
BplI GAGNNNNNCTC 2 cut(s) 992, 1024
BpuMI CCSGG 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 905
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 335, 393
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 1 cut(s) 934
BseBI CCWGG 3 cut(s) 590, 623, 906
BseDI CCNNGG 1 cut(s) 905
BseGI GGATG 5 cut(s) 529, 598, 784, 788, 822
BseLI CCNNNNNNNGG 3 cut(s) 17, 335, 393
BseMII CTCAG 4 cut(s) 482, 546, 872, 1059
BseNI ACTGG 1 cut(s) 934
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 4 cut(s) 48, 302, 366, 633
BshFI GGCC 3 cut(s) 434, 491, 520
BsiHKAI GWGCWC 1 cut(s) 1067
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 3 cut(s) 29, 492, 521
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 3 cut(s) 17, 335, 393
BsmAI GTCTC 2 cut(s) 193, 551
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 3 cut(s) 434, 491, 520
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 1 cut(s) 1067
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 2 cut(s) 232, 595
BspACI CCGC 1 cut(s) 502
BspANI GGCC 3 cut(s) 434, 491, 520
BspCNI CTCAG 4 cut(s) 481, 547, 873, 1058
BspTNI GGTCTC 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 491
BsrGI TGTACA 1 cut(s) 144
BsrI ACTGG 1 cut(s) 934
BssAI RCCGGY 1 cut(s) 491
BssECI CCNNGG 1 cut(s) 905
BssMI GATC 2 cut(s) 232, 595
BssNAI GTATAC 2 cut(s) 326, 680
Bst1107I GTATAC 2 cut(s) 326, 680
Bst2UI CCWGG 3 cut(s) 590, 623, 906
Bst4CI ACNGT 3 cut(s) 94, 743, 874
BstAUI TGTACA 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 454, 493
BstDEI CTNAG 4 cut(s) 468, 555, 881, 1045
BstF5I GGATG 5 cut(s) 529, 598, 784, 788, 822
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 2 cut(s) 235, 598
BstMAI GTCTC 2 cut(s) 193, 551
BstMBI GATC 2 cut(s) 232, 595
BstMWI GCNNNNNNNGC 1 cut(s) 501
BstNI CCWGG 3 cut(s) 590, 623, 906
BstSCI CCNGG 4 cut(s) 27, 588, 621, 904
BstV1I GCAGC 4 cut(s) 48, 302, 366, 633
BstZ17I GTATAC 2 cut(s) 326, 680
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 3 cut(s) 434, 491, 520
BtgZI GCGATG 1 cut(s) 962
BtrI CACGTC 1 cut(s) 17
BtsCI GGATG 5 cut(s) 529, 598, 784, 788, 822
Cac8I GCNNGC 2 cut(s) 454, 493
CfoI GCGC 1 cut(s) 646
Cfr10I RCCGGY 1 cut(s) 491
CsiI ACCWGGT 1 cut(s) 621
Csp6I GTAC 4 cut(s) 145, 700, 1006, 1042
CviAII CATG 1 cut(s) 1052
CviQI GTAC 4 cut(s) 145, 700, 1006, 1042
DdeI CTNAG 4 cut(s) 468, 555, 881, 1045
DpnI GATC 2 cut(s) 234, 597
DpnII GATC 2 cut(s) 232, 595
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 1 cut(s) 489
Eco31I GGTCTC 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 366
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 3 cut(s) 588, 621, 904
EcoT22I ATGCAT 1 cut(s) 1057
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 1 cut(s) 1055
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 541
FatI CATG 1 cut(s) 1051
FauNDI CATATG 2 cut(s) 609, 920
FbaI TGATCA 2 cut(s) 232, 595
FblI GTMKAC 2 cut(s) 325, 679
Fnu4HI GCNGC 4 cut(s) 62, 316, 380, 647
FokI GGATG 5 cut(s) 536, 605, 791, 795, 829
Fsp4HI GCNGC 4 cut(s) 62, 316, 380, 647
FspBI CTAG 1 cut(s) 321
GlaI GCGC 1 cut(s) 645
GluI GCNGC 4 cut(s) 62, 316, 380, 647
HaeIII GGCC 3 cut(s) 434, 491, 520
HapII CCGG 3 cut(s) 29, 492, 521
HhaI GCGC 1 cut(s) 646
Hin1II CATG 1 cut(s) 1055
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HinfI GANTC 7 cut(s) 152, 194, 364, 409, 551, 754, 773
HpaII CCGG 3 cut(s) 29, 492, 521
HphI GGTGA 3 cut(s) 440, 491, 944
Hpy166II GTNNAC 5 cut(s) 101, 326, 343, 657, 680
Hpy188III TCNNGA 3 cut(s) 82, 268, 368
Hpy8I GTNNAC 5 cut(s) 101, 326, 343, 657, 680
HpyAV CCTTC 3 cut(s) 53, 232, 265
HpyCH4III ACNGT 3 cut(s) 94, 743, 874
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 3 cut(s) 456, 649, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 501
HpyF3I CTNAG 4 cut(s) 468, 555, 881, 1045
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 1 cut(s) 1055
HspAI GCGC 1 cut(s) 644
KroI GCCGGC 1 cut(s) 491
KroNI GCCGGC 1 cut(s) 493
Ksp22I TGATCA 2 cut(s) 232, 595
Kzo9I GATC 2 cut(s) 232, 595
LmnI GCTCC 2 cut(s) 84, 1062
Lsp1109I GCAGC 4 cut(s) 48, 302, 366, 633
LweI GCATC 2 cut(s) 302, 773
MabI ACCWGGT 1 cut(s) 621
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 3 cut(s) 72, 353, 406
MalI GATC 2 cut(s) 234, 597
MboI GATC 2 cut(s) 232, 595
MboII GAAGA 5 cut(s) 175, 727, 832, 1027, 1065
MhlI GDGCHC 1 cut(s) 1067
MluCI AATT 5 cut(s) 4, 48, 251, 457, 734
MlyI GAGTC 4 cut(s) 188, 358, 403, 782
MmeI TCCRAC 3 cut(s) 424, 750, 756
MnlI CCTC 7 cut(s) 51, 178, 393, 424, 564, 705, 844
Mph1103I ATGCAT 1 cut(s) 1057
MroNI GCCGGC 1 cut(s) 491
MroXI GAANNNNTTC 1 cut(s) 939
MseI TTAA 7 cut(s) 69, 282, 375, 477, 534, 891, 1084
MspA1I CMGCKG 1 cut(s) 142
MspI CCGG 3 cut(s) 29, 492, 521
MspR9I CCNGG 4 cut(s) 29, 590, 623, 906
MvaI CCWGG 3 cut(s) 590, 623, 906
MwoI GCNNNNNNNGC 1 cut(s) 501
NaeI GCCGGC 1 cut(s) 493
NciI CCSGG 1 cut(s) 29
NdeI CATATG 2 cut(s) 609, 920
NdeII GATC 2 cut(s) 232, 595
NgoMIV GCCGGC 1 cut(s) 491
NlaIII CATG 1 cut(s) 1055
NmuCI GTSAC 2 cut(s) 72, 406
NsiI ATGCAT 1 cut(s) 1057
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 985
PdiI GCCGGC 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 939
PfeI GAWTC 3 cut(s) 152, 551, 754
PflFI GACNNNGTC 1 cut(s) 35
PflMI CCANNNNNTGG 1 cut(s) 17
PfoI TCCNGGA 2 cut(s) 27, 588
PkrI GCNGC 4 cut(s) 63, 317, 381, 648
PleI GAGTC 4 cut(s) 188, 358, 403, 781
PpsI GAGTC 4 cut(s) 188, 358, 403, 781
Psp6I CCWGG 3 cut(s) 588, 621, 904
PspGI CCWGG 3 cut(s) 588, 621, 904
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 142
RsaI GTAC 4 cut(s) 146, 701, 1007, 1043
RsaNI GTAC 4 cut(s) 145, 700, 1006, 1042
SaqAI TTAA 7 cut(s) 69, 282, 375, 477, 534, 891, 1084
SatI GCNGC 4 cut(s) 62, 316, 380, 647
Sau3AI GATC 2 cut(s) 232, 595
SchI GAGTC 4 cut(s) 188, 358, 403, 782
ScrFI CCNGG 4 cut(s) 29, 590, 623, 906
SduI GDGCHC 1 cut(s) 1067
SetI ASST 9 cut(s) 19, 144, 189, 288, 331, 381, 627, 1025, 1051
SexAI ACCWGGT 1 cut(s) 621
SfaNI GCATC 2 cut(s) 302, 773
Sse9I AATT 5 cut(s) 4, 48, 251, 457, 734
SsiI CCGC 1 cut(s) 502
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 4 cut(s) 27, 588, 621, 904
TaaI ACNGT 3 cut(s) 94, 743, 874
TaiI ACGT 1 cut(s) 19
TaqI TCGA 2 cut(s) 56, 212
TasI AATT 5 cut(s) 4, 48, 251, 457, 734
TatI WGTACW 2 cut(s) 144, 1005
TfiI GAWTC 3 cut(s) 152, 551, 754
Tru1I TTAA 7 cut(s) 69, 282, 375, 477, 534, 891, 1084
Tru9I TTAA 7 cut(s) 69, 282, 375, 477, 534, 891, 1084
TseFI GTSAC 2 cut(s) 72, 406
TseI GCWGC 4 cut(s) 61, 315, 379, 646
Tsp45I GTSAC 2 cut(s) 72, 406
TspDTI ATGAA 7 cut(s) 17, 124, 165, 626, 747, 833, 965
Tth111I GACNNNGTC 1 cut(s) 35
Van91I CCANNNNNTGG 1 cut(s) 17
XapI RAATTY 3 cut(s) 4, 48, 251
XmiI GTMKAC 2 cut(s) 325, 679
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.