FvH4_4g20770

Nuclear export mediator factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013357
Physical Location & Seq
Forward (+)
23905373 .. 23912237
6865 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20770.t1

Sequence Viewer

Length: 3408 bp
ATGGTGAAGGTGCGGATGAACACGGCCGACGTGGCCGCCGAGGTCAAGTGCTTACGCCGCCTCATCGGCATGCGCTGCGCCAATGTCTACGATCTCTCTCCCAAGACCTACATGCTGAAGCTCATGAACAGCAGCGGCGTCACCGAGTCCGGCGAGAGCGAGAAGGTCTTCTTGCTCATCGAAAGCGGCGTCAGATTGCACACTACTGCCTATGTTCGGGACAAGAGTAATACTCCGTCTGGATTTACGCTCAAAATAAGGAAGCATATACGAACAAGAAGGCTGGAGGATGTGAGGCAGCTCGGATATGATCGGATTATAATGTTTCAGTTTGGACTTGGTGCTAATGCCTACTATGTTATACTGGAGCTGTATGCGCAAGGAAATATTATACTAGCGGATTCGGAGTATATGGTCATGACTCTTCTTCGCTCGCATAGGGATGATGATAAAGGGGTTGCAATTATGTCACGCCATCGTTACCCAATAGAGATTTGTCGAACTTTTGAGCGAACAACTTCTGCAAAGTTGCAGGAAGCCCTTACATATTCCAAAGAACCAGATAAAAGTGAACCTGTTAAAGATTCTGAGGGTGGAAATGAGGCATCTGATGTGGCGAAAGAAAAAAAGGGGGGCAAGAAAGGTGGGAAGCCTGTCGAGTCAAGTAAAAAGTCTGGTGATGCTAAAGCCAAACATGCCACTTTGAAGAATGTTCTAGGGGACGGACTGGGTTATGGGCCTGCTCTTTCTGAGCATATTATTTTGGATGCTGGTCTTGTTCCAAATGCTAAAGTTGGTAAAGATGAAAAGCTAGATGACAATACACTAAAGCTTTTGCTCGAAGCTGTTGCAAAATTTGAGGACTGGCTGCATGATGTTATATCTGGCGAAAAAGTTCCTGAAGGGTACATTTTGATGCAGAACAAAAATTCAGGGAAGAATGGTTCCCCATCTGAACCAGGAAGTTCTGTTCAGATATATGATGAATTCTGCCCCTTACTATTGAACCAATTCAAGTTGAGGGAGTATGTGCAGTTTGAAACATTTGATGCATGTTTGGATGAGTTCTATAGCAAAATCGAAAGTCAAAGGTCAGAGCAGCAGCAAAAGGCAAAAGAGAGCTCTGCCACCCAGAGGCTTAATAAAATCCGTGTGGATCAGGAAAATCGTGTGCATATGCTAAGGAAAGAGGTTGACCAATGTGTTAAAATGGCGGAATTGATAGAATATAATTTAGAAGATGTGGATGCCGCTATATTAGCTGTTCGTGTAGCTCTTGCAAAGGGCATGAGTTGGGAGGATCTTGCTCGTATGGTAAAGGAGGAGAAAAAATCTGGAAATCCTATTGCCGGACTCATTGACAAACTTTATCTTGAAAGAAATTGCATGACTTTGCTTTTGAGCAACAATCTTGATGAAATGGATGATGATGAGAAGACTCTCCCTGCAGATAAGGTTGAAGTTGATATAGCGCTTTCTGCTCATGCCAATGCTCGGCGGTGGTATGAACTAAAGAAAAGCAAGGAGAGCAAACAGGAGAAGACTGTAACTGCACATGAAAAAGCGTTTAAAGCTGCTGAAAGAAAGACTCGTCTGCAGCTGTCACAGGAAAAAGCTGTTGCTACCATTTCACATATGCGCAAGGTTCACTGGTTTGAGAAATTTAACTGGTTCATCAGCAGTGAAAACTATTTGGTTATCAGTGGACGTGATGCTCAACAAAATGAGATGATAGTCAAGCGCTACATGTCGAAAGGAGATCTGTATGTCCATGCAGATTTGCATGGTGCTTCTAGTACTGTAATAAAGAACCATAGGCCTGAACAACCAGTGCCTCCACTTACTTTAAATCAAGCTGGATGCTACACAGTTTGCCAAAGTGCGGCGTGGGACTCAAAGATGGTCACTAGTGCTTGGTGGGTTTATCCTCACCAAGTCAGTAAAACTGCTCCTACAGGAGAGTATCTTACAGTTGGAAGTTTCATGATTCGTGGGAAGAAAAACTTCCTTCCCCCACACCCCCTGATTATGGGCTTTGGGCTGTTGTTTCGGTTGGATGAGAGCTCCTTGGGTTCACATCTGAATGAGAGGAGGGTAAGAGGTGAGGAGGAAGGGACAAATGATGCTGATGAGAGTGGGCCTTTAAGTGAGGTTTCTGATTCTGAGTCGGAGAAGGATTTGAGAGAGGAAAAACTTCCAGGAGAATTAGAAAGTGTACAAGATTCATCTAAACACGTACATCAACCAGACCATATATCCTCTCTGAATAGCTTACCGACAACTGTTACTAAACCAGTGGACTCAAATGAAAGTTCCTTGAAAAACAGGAACATCTTGAATGATGTTGATCAAGAGAACGTAGTTGATGTTGCTATGGATGGTGTTCCATCTGTCACTCCACAACTTGAGGACCTTATTGATAGAGCTCTTGGGCTTGGATCTGCATCTATGTCTGGTAATAAGTATAAATTTGAGACATCTCCAGTTGATTTGGTAGTAGAACATAATGTTGAAGAGAATAAGGCTACAGAAAAAGAGAAAGCTTATATCTCGAAAGCTGAAAGAAGAAAGCTCAAGAAAGGCCAGAGCGTTCCTGAAGATGTTAAGCCGAAATTAGAAAAAGTGAAAGAGAATGTTTCTGTCTGTCTGCCTGAAAAGGAAGTTAATGATAAGAAGCCAGGTGGTGGAAAAACCAGCCGTGGACAGAAGGGTAAACTCAAGAAGATAAAAGAGAAATATGCCGATCAGGATGAGGAAGAAAGAAGGATTCGTATGGCTTTACTAGCTTCTGCTGGAAATGTACAGAAGAAAGGAGAGGCACAAAACGGAGAGATAGCTCCAGTGGTGGACAAGAAACCTGGTCCTGAAGAGGGTGCAAAAATATGTTATAAATGTAAGAAGGTAGGTCATCTGTCTCGAGATTGCCAAGAACATCAAGTTGATACATCAGACAGACATGCAAATGGTGGAGTTGATGAGGAATCTAATGCCTTGGATAAAGCTACTTCTGAACTAGACAAGGTTACAATCGAGGAGGATGATATTCATGAGATAGGTGAAGAGGAGAAAGAAAAATTGAATGATGTGGATTACTTGACTGGCAATCCACTGCCCAGTGATATTCTCTTGTATGCGGTGCCTGTATGTGGTCCTTACAACGCAGTCCAATCTTACAAATATCGAGTGAAGATAATTCCAGGCTCTGTGAAGAGAGGAAAAGGTGCAAAAACTGCCATGAACTTGTTTAGCCACATGCCGGACGCAACTGCTAGAGAGAAAGAGTTGATGAAAGCGTGTACTGATCCTGAGCTGGTTGCTGCAATTATTGGAAATGTGAAGATTACAGCTGCAGGTCTCTCTCAATTGAAGCAGAAGCAAAAGAAAGTCAAGAAGAATACAGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006913 GO:0008150 GO:0046907 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3441 DUF814 FbpA zf-CCHC

Protein Analysis

1136

Amino Acids

126.83

Weight (kDa)

6.86

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT_N PF05833 19 - 149 4.3e-13 NFACT N-terminal and middle domains
NFACT_N PF05833 232 - 518 8.3e-09 NFACT N-terminal and middle domains
NFACT-R_1 PF05670 554 - 665 6.5e-41 NFACT protein RNA binding domain
NFACT-C PF11923 1022 - 1115 9.3e-27 NFACT protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013357)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49930
fragaria_vesca FvH4_4g20770 FvH4_4g20770 FvH4_4g20770
malus_domestica MD13G1148000.v1.1 MD16G1147800.v1.1
prunus_persica Prupe.1G111500_v2.0.a1
pyrus_communis pycom13g12740 pycom16g12630
rosa_chinensis RchiOBHm_Chr4g0426211
rosa_laevigata RLG00000007310
rosa_roxburghii Rroxscaffold_5G00368230
rosa_rugosa Rorug04G0209600
rosa_samantha Rh4AG266500 Rh4BG272500 Rh4CG285700 Rh4DG268800
rosa_wichuraiana Rw4G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 320, 2889
Acc16I TGCGCA 2 cut(s) 378, 1640
Acc36I ACCTGC 1 cut(s) 3341
AccB1I GGYRCC 1 cut(s) 3136
AccB7I CCANNNNNTGG 1 cut(s) 2684
AccI GTMKAC 1 cut(s) 87
AclWI GGATC 4 cut(s) 1164, 1308, 2446, 3296
AcoI YGGCCR 2 cut(s) 24, 33
AcsI RAATTY 5 cut(s) 854, 928, 986, 1661, 2468
AcuI CTGAAG 4 cut(s) 137, 921, 2616, 2886
AcyI GRCGYC 2 cut(s) 138, 189
AfaI GTAC 6 cut(s) 908, 1798, 2217, 2238, 2802, 3298
AfeI AGCGCT 2 cut(s) 1473, 1742
AflIII ACRYGT 2 cut(s) 1746, 2233
AhlI ACTAGT 1 cut(s) 1907
AjiI CACGTC 2 cut(s) 31, 1709
AjnI CCWGG 5 cut(s) 958, 2197, 2677, 2857, 3196
AloI GAACNNNNNNTCC 4 cut(s) 928, 954, 960, 986
Alw21I GWGCWC 3 cut(s) 1124, 2066, 2428
Alw26I GTCTC 3 cut(s) 2468, 2919, 3359
AlwI GGATC 4 cut(s) 1164, 1308, 2446, 3296
AlwNI CAGNNNCTG 1 cut(s) 3203
Ama87I CYCGRG 1 cut(s) 2916
Aor51HI AGCGCT 2 cut(s) 1473, 1742
AoxI GGCC 6 cut(s) 24, 33, 737, 1817, 2138, 2581
ApoI RAATTY 5 cut(s) 854, 928, 986, 1661, 2468
ArsI GACNNNNNNTTYG 4 cut(s) 174, 206, 1381, 1413
Asp700I GAANNNNTTC 6 cut(s) 167, 517, 894, 1010, 2003, 2193
AspLEI GCGC 6 cut(s) 75, 80, 379, 1474, 1641, 1743
AspS9I GGNCC 5 cut(s) 737, 2138, 2410, 2861, 3149
AsuHPI GGTGA 6 cut(s) 16, 133, 689, 1922, 2114, 3068
AvaI CYCGRG 1 cut(s) 2916
AvaII GGWCC 3 cut(s) 2410, 2861, 3149
BanI GGYRCC 1 cut(s) 3136
BanII GRGCYC 3 cut(s) 1124, 2066, 2428
BbsI GAAGAC 3 cut(s) 160, 1442, 1547
Bbv12I GWGCWC 3 cut(s) 1124, 2066, 2428
BccI CCATC 5 cut(s) 483, 958, 1894, 2372, 2395
BceAI ACGGC 2 cut(s) 39, 2682
BcgI CGANNNNNNTGC 2 cut(s) 830, 864
BciT130I CCWGG 5 cut(s) 960, 2199, 2679, 2859, 3198
BclI TGATCA 1 cut(s) 2347
BcoDI GTCTC 3 cut(s) 2468, 2919, 3359
BcuI ACTAGT 1 cut(s) 1907
BfaI CTAG 8 cut(s) 395, 716, 812, 1794, 1908, 2783, 3014, 3270
BfmI CTRYAG 6 cut(s) 1069, 1446, 1595, 1953, 2526, 3348
BfoI RGCGCY 2 cut(s) 1475, 1744
BfuAI ACCTGC 1 cut(s) 3341
BglI GCCNNNNNGGC 2 cut(s) 32, 66
BglII AGATCT 1 cut(s) 1759
BmcAI AGTACT 1 cut(s) 1798
Bme1390I CCNGG 5 cut(s) 960, 2199, 2679, 2859, 3198
Bme18I GGWCC 3 cut(s) 2410, 2861, 3149
BmeT110I CYCGRG 1 cut(s) 2916
BmgBI CACGTC 2 cut(s) 31, 1709
BmgT120I GGNCC 5 cut(s) 737, 2138, 2410, 2861, 3149
BmiI GGNNCC 2 cut(s) 946, 3138
BmrFI CCNGG 5 cut(s) 960, 2199, 2679, 2859, 3198
BmrI ACTGGG 2 cut(s) 737, 3108
BmuI ACTGGG 2 cut(s) 737, 3108
BpiI GAAGAC 3 cut(s) 160, 1442, 1547
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpmI CTGGAG 4 cut(s) 305, 386, 2466, 2823
Bpu10I CCTNAGC 2 cut(s) 1181, 3306
BpuEI CTTGAG 3 cut(s) 2426, 2558, 2702
BsaAI YACGTR 1 cut(s) 2236
BsaBI GATNNNNATC 2 cut(s) 2346, 2443
BsaHI GRCGYC 2 cut(s) 138, 189
BsaI GGTCTC 1 cut(s) 3359
BsaJI CCNNGG 4 cut(s) 39, 2067, 2698, 2991
Bse8I GATNNNNATC 2 cut(s) 2346, 2443
BseBI CCWGG 5 cut(s) 960, 2199, 2679, 2859, 3198
BseDI CCNNGG 4 cut(s) 39, 2067, 2698, 2991
BseJI GATNNNNATC 2 cut(s) 2346, 2443
BseMII CTCAG 4 cut(s) 579, 741, 2154, 3297
BseRI GAGGAG 5 cut(s) 1337, 2104, 2120, 3047, 3077
BseX3I CGGCCG 1 cut(s) 24
BsgI GTGCAG 2 cut(s) 1052, 1536
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 6 cut(s) 26, 35, 739, 1819, 2140, 2583
BshNI GGYRCC 1 cut(s) 3136
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 3 cut(s) 1124, 2066, 2428
BsiHKCI CYCGRG 1 cut(s) 2916
BsiSI CCGG 3 cut(s) 150, 1350, 3257
BslFI GGGAC 4 cut(s) 233, 734, 1904, 2128
BsmAI GTCTC 3 cut(s) 2468, 2919, 3359
BsmFI GGGAC 4 cut(s) 233, 734, 1904, 2128
BsnI GGCC 6 cut(s) 26, 35, 739, 1819, 2140, 2583
Bso31I GGTCTC 1 cut(s) 3359
BsoBI CYCGRG 1 cut(s) 2916
Bsp1286I GDGCHC 3 cut(s) 1124, 2066, 2428
Bsp1407I TGTACA 2 cut(s) 2215, 2800
Bsp143I GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
BspANI GGCC 6 cut(s) 26, 35, 739, 1819, 2140, 2583
BspCNI CTCAG 4 cut(s) 580, 742, 2155, 3298
BspHI TCATGA 4 cut(s) 123, 417, 1983, 3046
BspLI GGNNCC 2 cut(s) 946, 3138
BspMAI CTGCAG 3 cut(s) 1450, 1599, 3352
BspMI ACCTGC 1 cut(s) 3341
BspPI GGATC 4 cut(s) 1164, 1308, 2446, 3296
BspT107I GGYRCC 1 cut(s) 3136
BspTNI GGTCTC 1 cut(s) 3359
BsrGI TGTACA 2 cut(s) 2215, 2800
BssECI CCNNGG 4 cut(s) 39, 2067, 2698, 2991
BssMI GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
BssNI GRCGYC 2 cut(s) 138, 189
BssT1I CCWWGG 2 cut(s) 2067, 2991
Bst2UI CCWGG 5 cut(s) 960, 2199, 2679, 2859, 3198
Bst4CI ACNGT 5 cut(s) 1546, 1801, 1870, 1972, 2284
Bst6I CTCTTC 5 cut(s) 429, 2508, 2862, 3054, 3203
BstACI GRCGYC 2 cut(s) 138, 189
BstAPI GCANNNNNTGC 2 cut(s) 75, 3230
BstAUI TGTACA 2 cut(s) 2215, 2800
BstBAI YACGTR 1 cut(s) 2236
BstC8I GCNNGC 3 cut(s) 71, 434, 741
BstDEI CTNAG 5 cut(s) 588, 750, 1181, 2163, 3306
BstDSI CCRYGG 1 cut(s) 2698
BstH2I RGCGCY 2 cut(s) 1475, 1744
BstHHI GCGC 6 cut(s) 75, 80, 379, 1474, 1641, 1743
BstKTI GATC 9 cut(s) 94, 313, 1159, 1303, 1762, 2350, 2441, 2746, 3304
BstMAI GTCTC 3 cut(s) 2468, 2919, 3359
BstMBI GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 5 cut(s) 960, 2199, 2679, 2859, 3198
BstNSI RCATGY 7 cut(s) 73, 115, 698, 1056, 1750, 2960, 3256
BstSCI CCNGG 5 cut(s) 958, 2197, 2677, 2857, 3196
BstSFI CTRYAG 6 cut(s) 1069, 1446, 1595, 1953, 2526, 3348
BstV2I GAAGAC 3 cut(s) 160, 1442, 1547
BstX2I RGATCY 3 cut(s) 1300, 1759, 2438
BstYI RGATCY 3 cut(s) 1300, 1759, 2438
BstZI CGGCCG 1 cut(s) 24
BsuRI GGCC 6 cut(s) 26, 35, 739, 1819, 2140, 2583
BtgI CCRYGG 1 cut(s) 2698
BtrI CACGTC 2 cut(s) 31, 1709
BtsI GCAGTG 2 cut(s) 1687, 3107
BtsIMutI CAGTG 8 cut(s) 1648, 1687, 1708, 1836, 2301, 2847, 3107, 3121
BveI ACCTGC 1 cut(s) 3341
Cac8I GCNNGC 3 cut(s) 71, 434, 741
CaiI CAGNNNCTG 1 cut(s) 3203
CciI TCATGA 4 cut(s) 123, 417, 1983, 3046
CfoI GCGC 6 cut(s) 75, 80, 379, 1474, 1641, 1743
Cfr13I GGNCC 5 cut(s) 737, 2138, 2410, 2861, 3149
CseI GACGC 3 cut(s) 127, 178, 3269
CsiI ACCWGGT 1 cut(s) 2857
Csp6I GTAC 6 cut(s) 907, 1797, 2216, 2237, 2801, 3297
CspCI CAANNNNNGTGG 2 cut(s) 625, 660
CviQI GTAC 6 cut(s) 907, 1797, 2216, 2237, 2801, 3297
DdeI CTNAG 5 cut(s) 588, 750, 1181, 2163, 3306
DpnI GATC 9 cut(s) 93, 312, 1158, 1302, 1761, 2349, 2440, 2745, 3303
DpnII GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
DraI TTTAAA 2 cut(s) 1570, 1848
EaeI YGGCCR 2 cut(s) 24, 33
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 5 cut(s) 429, 2508, 2862, 3054, 3203
EarI CTCTTC 5 cut(s) 429, 2508, 2862, 3054, 3203
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 3 cut(s) 1122, 2064, 2426
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 2 cut(s) 2067, 2991
Eco147I AGGCCT 1 cut(s) 1819
Eco24I GRGCYC 3 cut(s) 1124, 2066, 2428
Eco31I GGTCTC 1 cut(s) 3359
Eco47I GGWCC 3 cut(s) 2410, 2861, 3149
Eco47III AGCGCT 2 cut(s) 1473, 1742
Eco52I CGGCCG 1 cut(s) 24
Eco53kI GAGCTC 3 cut(s) 1122, 2064, 2426
Eco57I CTGAAG 4 cut(s) 137, 921, 2616, 2886
Eco88I CYCGRG 1 cut(s) 2916
EcoICRI GAGCTC 3 cut(s) 1122, 2064, 2426
EcoO109I RGGNCCY 1 cut(s) 2410
EcoRI GAATTC 1 cut(s) 986
EcoRII CCWGG 5 cut(s) 958, 2197, 2677, 2857, 3196
EcoT14I CCWWGG 2 cut(s) 2067, 2991
EcoT22I ATGCAT 1 cut(s) 1054
EcoT38I GRGCYC 3 cut(s) 1124, 2066, 2428
ErhI CCWWGG 2 cut(s) 2067, 2991
FalI AAGNNNNNCTT 4 cut(s) 155, 187, 1988, 2020
FaqI GGGAC 4 cut(s) 233, 734, 1904, 2128
FauNDI CATATG 2 cut(s) 1176, 1635
FbaI TGATCA 1 cut(s) 2347
FblI GTMKAC 1 cut(s) 87
FriOI GRGCYC 3 cut(s) 1124, 2066, 2428
FspBI CTAG 8 cut(s) 395, 716, 812, 1794, 1908, 2783, 3014, 3270
FspI TGCGCA 2 cut(s) 378, 1640
GlaI GCGC 6 cut(s) 74, 79, 378, 1473, 1640, 1742
GsuI CTGGAG 4 cut(s) 305, 386, 2466, 2823
HaeII RGCGCY 2 cut(s) 1475, 1744
HaeIII GGCC 6 cut(s) 26, 35, 739, 1819, 2140, 2583
HapII CCGG 3 cut(s) 150, 1350, 3257
HgaI GACGC 3 cut(s) 127, 178, 3269
HhaI GCGC 6 cut(s) 75, 80, 379, 1474, 1641, 1743
Hin1I GRCGYC 2 cut(s) 138, 189
Hin6I GCGC 6 cut(s) 73, 78, 377, 1472, 1639, 1741
HinP1I GCGC 6 cut(s) 73, 78, 377, 1472, 1639, 1741
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 2 cut(s) 830, 2541
HpaII CCGG 3 cut(s) 150, 1350, 3257
HphI GGTGA 6 cut(s) 16, 133, 689, 1922, 2114, 3068
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 9 cut(s) 157, 273, 896, 2018, 2105, 2167, 2701, 2757, 2893
HpyCH4III ACNGT 5 cut(s) 1546, 1801, 1870, 1972, 2284
HpyCH4IV ACGT 4 cut(s) 30, 1708, 2235, 2358
HpyF3I CTNAG 5 cut(s) 588, 750, 1181, 2163, 3306
HpySE526I ACGT 4 cut(s) 30, 1708, 2235, 2358
Hsp92I GRCGYC 2 cut(s) 138, 189
HspAI GCGC 6 cut(s) 73, 78, 377, 1472, 1639, 1741
Ksp22I TGATCA 1 cut(s) 2347
Kzo9I GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
LmnI GCTCC 4 cut(s) 367, 1954, 2069, 2842
MabI ACCWGGT 1 cut(s) 2857
MaeI CTAG 8 cut(s) 395, 716, 812, 1794, 1908, 2783, 3014, 3270
MaeII ACGT 4 cut(s) 30, 1708, 2235, 2358
MaeIII GTNAC 9 cut(s) 139, 468, 479, 1546, 1602, 1903, 2284, 2392, 3022
MalI GATC 9 cut(s) 93, 312, 1158, 1302, 1761, 2349, 2440, 2745, 3303
MboI GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
MfeI CAATTG 1 cut(s) 3362
MflI RGATCY 3 cut(s) 1300, 1759, 2438
MhlI GDGCHC 3 cut(s) 1124, 2066, 2428
MlyI GAGTC 9 cut(s) 155, 415, 668, 1347, 1432, 1582, 1886, 2174, 2294
MmeI TCCRAC 3 cut(s) 1954, 2034, 2148
Mph1103I ATGCAT 1 cut(s) 1054
MroXI GAANNNNTTC 6 cut(s) 167, 517, 894, 1010, 2003, 2193
MseI TTAA 9 cut(s) 579, 1140, 1206, 1569, 1665, 1847, 2144, 2604, 2664
MslI CAYNNNNRTG 6 cut(s) 68, 441, 914, 1488, 2082, 2961
MspA1I CMGCKG 3 cut(s) 135, 1600, 3347
MspI CCGG 3 cut(s) 150, 1350, 3257
MspR9I CCNGG 5 cut(s) 960, 2199, 2679, 2859, 3198
MteI GCGCNGCGC 1 cut(s) 76
MunI CAATTG 1 cut(s) 3362
MvaI CCWGG 5 cut(s) 960, 2199, 2679, 2859, 3198
NdeI CATATG 2 cut(s) 1176, 1635
NdeII GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
NlaIV GGNNCC 2 cut(s) 946, 3138
NmeAIII GCCGAG 2 cut(s) 64, 1474
NmuCI GTSAC 5 cut(s) 139, 468, 1602, 1903, 2392
NsbI TGCGCA 2 cut(s) 378, 1640
NsiI ATGCAT 1 cut(s) 1054
NspI RCATGY 7 cut(s) 73, 115, 698, 1056, 1750, 2960, 3256
PaeI GCATGC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 2916
PagI TCATGA 4 cut(s) 123, 417, 1983, 3046
PceI AGGCCT 1 cut(s) 1819
PciI ACATGT 1 cut(s) 1746
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 186
PdmI GAANNNNTTC 6 cut(s) 167, 517, 894, 1010, 2003, 2193
PfeI GAWTC 7 cut(s) 401, 584, 1987, 2159, 2222, 2767, 2981
PflMI CCANNNNNTGG 1 cut(s) 2684
PfoI TCCNGGA 1 cut(s) 2197
PleI GAGTC 9 cut(s) 154, 415, 667, 1347, 1432, 1582, 1886, 2173, 2294
PpsI GAGTC 9 cut(s) 154, 415, 667, 1347, 1432, 1582, 1886, 2173, 2294
Ppu21I YACGTR 1 cut(s) 2236
PpuMI RGGWCCY 1 cut(s) 2410
PscI ACATGT 1 cut(s) 1746
PsiI TTATAA 2 cut(s) 320, 2889
Psp124BI GAGCTC 3 cut(s) 1124, 2066, 2428
Psp5II RGGWCCY 1 cut(s) 2410
Psp6I CCWGG 5 cut(s) 958, 2197, 2677, 2857, 3196
PspGI CCWGG 5 cut(s) 958, 2197, 2677, 2857, 3196
PspN4I GGNNCC 2 cut(s) 946, 3138
PspPI GGNCC 5 cut(s) 737, 2138, 2410, 2861, 3149
PspPPI RGGWCCY 1 cut(s) 2410
PstI CTGCAG 3 cut(s) 1450, 1599, 3352
PstNI CAGNNNCTG 1 cut(s) 3203
PsuI RGATCY 3 cut(s) 1300, 1759, 2438
PvuII CAGCTG 2 cut(s) 1600, 3347
RsaI GTAC 6 cut(s) 908, 1798, 2217, 2238, 2802, 3298
RsaNI GTAC 6 cut(s) 907, 1797, 2216, 2237, 2801, 3297
RseI CAYNNNNRTG 6 cut(s) 68, 441, 914, 1488, 2082, 2961
SacI GAGCTC 3 cut(s) 1124, 2066, 2428
SaqAI TTAA 9 cut(s) 579, 1140, 1206, 1569, 1665, 1847, 2144, 2604, 2664
Sau3AI GATC 9 cut(s) 91, 310, 1156, 1300, 1759, 2347, 2438, 2743, 3301
Sau96I GGNCC 5 cut(s) 737, 2138, 2410, 2861, 3149
ScaI AGTACT 1 cut(s) 1798
SchI GAGTC 9 cut(s) 155, 415, 668, 1347, 1432, 1582, 1886, 2174, 2294
ScrFI CCNGG 5 cut(s) 960, 2199, 2679, 2859, 3198
SduI GDGCHC 3 cut(s) 1124, 2066, 2428
SexAI ACCWGGT 1 cut(s) 2857
SfcI CTRYAG 6 cut(s) 1069, 1446, 1595, 1953, 2526, 3348
SfiI GGCCNNNNNGGCC 1 cut(s) 32
Sfr274I CTCGAG 1 cut(s) 2916
SinI GGWCC 3 cut(s) 2410, 2861, 3149
SlaI CTCGAG 1 cut(s) 2916
SmiMI CAYNNNNRTG 6 cut(s) 68, 441, 914, 1488, 2082, 2961
SmlI CTYRAG 4 cut(s) 2405, 2573, 2717, 2916
SmoI CTYRAG 4 cut(s) 2405, 2573, 2717, 2916
SpeI ACTAGT 1 cut(s) 1907
SphI GCATGC 1 cut(s) 73
SseBI AGGCCT 1 cut(s) 1819
SspI AATATT 1 cut(s) 388
SspMI CTAG 8 cut(s) 395, 716, 812, 1794, 1908, 2783, 3014, 3270
SstI GAGCTC 3 cut(s) 1124, 2066, 2428
StuI AGGCCT 1 cut(s) 1819
StyD4I CCNGG 5 cut(s) 958, 2197, 2677, 2857, 3196
StyI CCWWGG 2 cut(s) 2067, 2991
TaaI ACNGT 5 cut(s) 1546, 1801, 1870, 1972, 2284
TaiI ACGT 4 cut(s) 33, 1711, 2238, 2361
TatI WGTACW 4 cut(s) 1796, 2215, 2800, 3296
TauI GCSGC 6 cut(s) 38, 60, 138, 189, 1253, 1886
TfiI GAWTC 7 cut(s) 401, 584, 1987, 2159, 2222, 2767, 2981
Tru1I TTAA 9 cut(s) 579, 1140, 1206, 1569, 1665, 1847, 2144, 2604, 2664
Tru9I TTAA 9 cut(s) 579, 1140, 1206, 1569, 1665, 1847, 2144, 2604, 2664
TscAI CASTG 8 cut(s) 1655, 1687, 1708, 1836, 2301, 2847, 3114, 3121
TseFI GTSAC 5 cut(s) 139, 468, 1602, 1903, 2392
Tsp45I GTSAC 5 cut(s) 139, 468, 1602, 1903, 2392
TspGWI ACGGA 4 cut(s) 225, 738, 1139, 2841
TspRI CASTG 8 cut(s) 1655, 1687, 1708, 1836, 2301, 2847, 3114, 3121
Van91I CCANNNNNTGG 1 cut(s) 2684
VpaK11BI GGWCC 3 cut(s) 2410, 2861, 3149
XapI RAATTY 5 cut(s) 854, 928, 986, 1661, 2468
XceI RCATGY 7 cut(s) 73, 115, 698, 1056, 1750, 2960, 3256
XhoI CTCGAG 1 cut(s) 2916
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 6 cut(s) 167, 517, 894, 1010, 2003, 2193
XspI CTAG 8 cut(s) 395, 716, 812, 1794, 1908, 2783, 3014, 3270
ZrmI AGTACT 1 cut(s) 1798
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.