Rw4G023040

Nuclear export mediator factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013357
Physical Location & Seq
Forward (+)
48618333 .. 48624803
6471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G023040.1

Sequence Viewer

Length: 3408 bp
ATGGTGAAGGTGCGGATGAACACGGCCGACGTGGCCGCCGAGGTCAAGTGCTTGAGGCGGCTTATCGGCATGCGCTGCGCCAATGTCTACGATCTCTCTCCCAAGACCTATATGTTGAAGCTTATGAACAGTAGCGGCGTCACCGAGTCCGGCGAGAGCGAGAAGGTCTTCTTGCTTATCGAAAGCGGTGTCAGATTGCACACTACTGCCTATGTTAGGGACAAGAGTAATACTCCGTCTGGTTTTACGCTCAAAATAAGGAAGCATATACGAACAAGAAGGCTCGAGGATGTGAGGCAGCTGGGATACGATCGGATTATACTATTTCAGTTTGGATTGGGTGCTAATGCCTACTATGTTATATTGGAGCTATATGCGCAAGGAAATATTATTCTTGCAGATTCCGAATATATGGTCATGACTCTTCTTCGCTCGCACAGGGATGATGACAAAGGGGTTGCAATTATGTCACGCCATCATTATCCAATAGAGATTTGTCGAGTTTTTGAGCGAACAACCTGTGCAAAGTTGCAGGAAGCCCTTACATATTCCAAGGAACCTGATAACAATGAATCTGTAAAAGATTCTGAAGGTCGAAATGATGCATCTGATGCGCCAAAAGAAAAAAAGGGTGGTCGTAAAGGTGGGAAGCCCGTCGAGTCCAGTAAAAAGCCTGGTGATGCTAAAGCCAAACAAGCCACTTTGAAGAATGTTCTAGGGGACGGACTGGGCTATGGACCTGCCCTTTCTGAGCATATTATTTTAGATGCTGGTCTTGTTCCAAATACTAAAGTTGGTAAAGATGAAAAGCTAGATGACAATACACTAAAGCTCTTGCTCGAAGCCATTACAAAATTTGAGGACTGGCTGCATGATGTTATATCCGGTGAAAAAGTTCCTGAAGGATACATTTTGATGCAGCACAAAAATTCAGGGAAGAATGGTCCCCCATCTGAACCAGGAAGTTCTGGCCAGATATATGATGAATTCTGCCCCATATTATTGAACCAATTCAAGTCGAGGGAGTATGTGCAGTTTGAAACATTTGATGCATCTTTGGATGAGTTCTATAGCAAAATTGAGAGTCAAAGGTCAGAGCAGCAGCAAAAAGCAAAAGAGAGCTCTGCCACCCAGAGGCTTAATAAAATCCGCGTGGATCAGGAAAATCGTGTGCATATGTTAAGGAAAGAGGTTGACCAATGTGTTAAAATGGCGGAATTGATAGAATATAATTTAGAAGATGTGGATGCTGCTATATTAGCTGTTCGTGTAGCTCTTGCAAAGGGCATGAGTTGGGAGGATCTTGCTCGTATGGTAAAGGAAGAGAAGAAATCTGGAAATCCTATTGCCGGACTCATTGACAAGCTTTATCTTGAAAGGAATTGCATGACATTGCTTTTGAGCAACAATCTTGATGAAATGGATGATGATGAGAAGACCCTCCCTGCAGATAAGGTGGAAGTTGATTTAGCGCTTTCTGCACATGCCAATGCTCGGCGGTGGTATGAACTAAAGAAAAGCAAGGAGAGCAAACAGGAGAAGACTGTAACAGCACATGAAAAAGCATTTAAAGCTGCCGAAAGAAAGACTCGTCTGCAGCTATCGCAGGAAAAAGCTGTTGCTACCATTTCACATATGCGCAAGGTTCACTGGTTTGAGAAATTTAACTGGTTCATCAGCAGTGAAAACTATTTGGTTATCAGTGGACGTGATGCTCAACAAAATGAGATGATAGTCAAGCGCTACATGTCAAAAGGAGATCTGTATGTTCATGCAGATTTGCATGGCGCTTCTAGTACTGTAGTAAAGAACCATAGGCCTGAACAACCAGTGCCTCCACTTACTTTAAATCAAGCTGGATGCTACACGGTTTGCCAAAGTGCGGCATGGGATTCAAAGATGGTCACTAGTGCTTGGTGGGTTTATCCTCACCAAGTCAGTAAAACTGCTCCCACAGGGGAGTATCTTACAGTTGGAAGTTTCATGATTCGTGGGAAGAAGAACTTCCTTCCTCCACACCCTCTGATTATGGGCTTTGGGCTGTTATTTCGGTTGGATGAGAGCTCCTTGGGTTCACATCTGAACGAGAGGAGGGTAAGAGGTGAGGAGGAAGGGACAAATGATGCTGATGAAAGTGGGCCTCTAAGTGAGGTTTCTGATTCTGAATCAGAGAAAGATTTGAGAGAGGAAAAAATTCTAGGGGAATCACAAAGTGTACCAGATTCATCTAAACATGTACATAAACCAGATGATATATCCTCTCAGAATAGCTTACCTACAACCGTTGCTAAACAAGTGGACTCAAATTCAACCAGGAACATCTTGAATGATGTTGATCAACACAACGCAGTTGACGTTGCTGTGAATGGTGTTGCACCTGTCACCCCACAACTTGAGGACCTCATTGATAGAGCTCTTGGGCTTGGATCTGCATCTATGTCTGTTAAAAATTATAAAGTTGAAACCTCTCCAGTTGATTCGGTAGCAGAACAAAATGTTGAAGAGAATAAGGCTACAGTAAAAGAGAAAGCTTATATCTCGAAAGCTGAAAGAAGAAAGCTCAAGAAAGGCCAGAGTGTTCCTGAAGATGTTAAACTGAAAGTAGATAAAGTGGAAGAAAATGTTTCTGTCCGTCAGTCTGAAAAGGAAGTAAATGATAAGAAGTCAGGTGGTGGAAAAACCAGCCGTGGACAGAGGGGTAAACTCAAGAAGATAAAAGAGAAATATGCTGATCAAGATGAGGAAGAAAGAAGGATTCGTATGGCTTTACTAGCCTCTGCTGGAAATGTACAGAAGAAAGGAGAGTCGCAAAATGGAGAGATAGCTCAAGGGAGGACAAGAAACCTGGTATTTTTATTTCATTGTCCTGAAGAGGGTGCAAAAATATGTTATAAATGTAAGAAGGTAGGTCATCTGTCCCGAGATTGCCAAGAACATCAAGTTGATACATCAGATAGTCATGCAAATGGTGGAGTTGATCACCCTAATGCCTTGGATAAAACTACTTCTGAACTAGACAAGGTGACAATTGAGGAGGATGATATTCATGAGATAGGTGAAGAGGAGAAGGAAAAATTAAATGATGTGGATTACTTGACTGGCAATCCACTGCCCAGTGATATTCTCTTGTATGCAGTGCCTGTATGTGGTCCTTATAATGCAGTCCAATCTTACAAATATCGAGTGAAGATAATTCCTGGCTCTGTGAAGAGAGGAAAAGGTGCAAAAACTGCCATGAACTTGTTTAGCCACATGCCGGACGCAACAGCTAGAGAGAAAGAGTTGATGAAAGCGTGTACGGATCCTGAGCTGGTTGCCGCAATTATTGGAAATGTCAAGATTACAGCTGCAGGCCTCTCTCAATTGAAGCAGAAGCAAAAGAAAGTCAAGAAGAACGCAGCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006913 GO:0008150 GO:0046907 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3441 DUF814 FbpA zf-CCHC

Protein Analysis

1135

Amino Acids

126.9

Weight (kDa)

7.56

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT_N PF05833 19 - 149 5.1e-13 NFACT N-terminal and middle domains
NFACT_N PF05833 231 - 518 2.9e-08 NFACT N-terminal and middle domains
NFACT-R_1 PF05670 554 - 665 7.2e-41 NFACT protein RNA binding domain
NFACT-C PF11923 1022 - 1115 9.3e-27 NFACT protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013357)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49930
fragaria_vesca FvH4_4g20770 FvH4_4g20770 FvH4_4g20770
malus_domestica MD13G1148000.v1.1 MD16G1147800.v1.1
prunus_persica Prupe.1G111500_v2.0.a1
pyrus_communis pycom13g12740 pycom16g12630
rosa_chinensis RchiOBHm_Chr4g0426211
rosa_laevigata RLG00000007310
rosa_roxburghii Rroxscaffold_5G00368230
rosa_rugosa Rorug04G0209600
rosa_samantha Rh4AG266500 Rh4BG272500 Rh4CG285700 Rh4DG268800
rosa_wichuraiana Rw4G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2454, 2892, 3156
Acc16I TGCGCA 2 cut(s) 378, 1640
Acc36I ACCTGC 1 cut(s) 748
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 1152
AclWI GGATC 5 cut(s) 1164, 1308, 2434, 3296, 3309
AcoI YGGCCR 3 cut(s) 24, 33, 970
AcsI RAATTY 6 cut(s) 854, 928, 986, 1661, 2193, 2305
AcuI CTGAAG 4 cut(s) 609, 921, 2604, 2889
AcyI GRCGYC 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 2213
AfaI GTAC 5 cut(s) 1798, 2217, 2238, 2790, 3298
AfeI AGCGCT 2 cut(s) 1473, 1742
AfiI CCNNNNNNNGG 8 cut(s) 216, 1134, 1349, 1494, 1882, 2873, 3146, 3256
AflIII ACRYGT 2 cut(s) 1746, 2233
AhlI ACTAGT 1 cut(s) 1907
AjiI CACGTC 2 cut(s) 31, 1709
AjnI CCWGG 5 cut(s) 673, 958, 2312, 2844, 3196
Alw21I GWGCWC 3 cut(s) 1124, 2066, 2416
AlwI GGATC 5 cut(s) 1164, 1308, 2434, 3296, 3309
AlwNI CAGNNNCTG 1 cut(s) 3140
Ama87I CYCGRG 2 cut(s) 284, 2919
Aor51HI AGCGCT 2 cut(s) 1473, 1742
AoxI GGCC 7 cut(s) 24, 33, 970, 1817, 2138, 2569, 3352
ApoI RAATTY 6 cut(s) 854, 928, 986, 1661, 2193, 2305
ArsI GACNNNNNNTTYG 6 cut(s) 174, 206, 399, 431, 1381, 1413
Asp700I GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
AspLEI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
AspS9I GGNCC 5 cut(s) 737, 944, 2138, 2398, 3149
AvaI CYCGRG 2 cut(s) 284, 2919
AvaII GGWCC 4 cut(s) 737, 944, 2398, 3149
BalI TGGCCA 1 cut(s) 972
BamHI GGATCC 1 cut(s) 3301
BanII GRGCYC 3 cut(s) 1124, 2066, 2416
BbsI GAAGAC 3 cut(s) 160, 1442, 1547
Bbv12I GWGCWC 3 cut(s) 1124, 2066, 2416
BccI CCATC 3 cut(s) 483, 958, 1894
BceAI ACGGC 2 cut(s) 39, 2670
BciT130I CCWGG 5 cut(s) 675, 960, 2314, 2846, 3198
BciVI GTATCC 2 cut(s) 299, 899
BclI TGATCA 3 cut(s) 2335, 2731, 2977
BcuI ACTAGT 1 cut(s) 1907
BfaI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2771, 3014, 3270
BfmI CTRYAG 6 cut(s) 1069, 1446, 1595, 1800, 2514, 3348
BfoI RGCGCY 3 cut(s) 1475, 1744, 1791
BfuAI ACCTGC 1 cut(s) 748
BfuI GTATCC 2 cut(s) 299, 899
BglI GCCNNNNNGGC 1 cut(s) 32
BglII AGATCT 1 cut(s) 1759
BmcAI AGTACT 1 cut(s) 1798
Bme1390I CCNGG 5 cut(s) 675, 960, 2314, 2846, 3198
Bme18I GGWCC 4 cut(s) 737, 944, 2398, 3149
BmeT110I CYCGRG 2 cut(s) 284, 2919
BmgBI CACGTC 2 cut(s) 31, 1709
BmgT120I GGNCC 5 cut(s) 737, 944, 2138, 2398, 3149
BmiI GGNNCC 3 cut(s) 558, 946, 3303
BmrFI CCNGG 5 cut(s) 675, 960, 2314, 2846, 3198
BmrI ACTGGG 2 cut(s) 737, 3108
BmuI ACTGGG 2 cut(s) 737, 3108
BpiI GAAGAC 3 cut(s) 160, 1442, 1547
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpmI CTGGAG 1 cut(s) 2454
Bpu10I CCTNAGC 1 cut(s) 3306
BpuEI CTTGAG 5 cut(s) 73, 2414, 2546, 2690, 2811
BsaBI GATNNNNATC 2 cut(s) 2334, 2431
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 5 cut(s) 39, 552, 2067, 2686, 2991
BsaWI WCCGGW 1 cut(s) 884
Bsc4I CCNNNNNNNGG 8 cut(s) 216, 1134, 1349, 1494, 1882, 2873, 3146, 3256
Bse1I ACTGG 9 cut(s) 663, 732, 869, 1655, 1673, 1829, 2471, 3103, 3114
Bse3DI GCAATG 1 cut(s) 1391
Bse8I GATNNNNATC 2 cut(s) 2334, 2431
BseBI CCWGG 5 cut(s) 675, 960, 2314, 2846, 3198
BseDI CCNNGG 5 cut(s) 39, 552, 2067, 2686, 2991
BseGI GGATG 9 cut(s) 21, 295, 448, 1066, 1252, 1429, 1865, 2062, 3043
BseJI GATNNNNATC 2 cut(s) 2334, 2431
BseLI CCNNNNNNNGG 8 cut(s) 216, 1134, 1349, 1494, 1882, 2873, 3146, 3256
BseMI GCAATG 1 cut(s) 1391
BseMII CTCAG 3 cut(s) 741, 2276, 3297
BseNI ACTGG 9 cut(s) 663, 732, 869, 1655, 1673, 1829, 2471, 3103, 3114
BseRI GAGGAG 4 cut(s) 2104, 2120, 3047, 3077
BseX3I CGGCCG 1 cut(s) 24
BseYI CCCAGC 1 cut(s) 301
BsgI GTGCAG 2 cut(s) 1052, 1464
Bsh1236I CGCG 1 cut(s) 1152
Bsh1285I CGRYCG 2 cut(s) 27, 313
BshFI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2571, 3354
BsiEI CGRYCG 2 cut(s) 27, 313
BsiHKAI GWGCWC 3 cut(s) 1124, 2066, 2416
BsiHKCI CYCGRG 2 cut(s) 284, 2919
BsiSI CCGG 4 cut(s) 150, 885, 1350, 3257
BslFI GGGAC 5 cut(s) 233, 734, 930, 2128, 2902
BslI CCNNNNNNNGG 8 cut(s) 216, 1134, 1349, 1494, 1882, 2873, 3146, 3256
BsmFI GGGAC 5 cut(s) 233, 734, 930, 2128, 2902
BsnI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2571, 3354
BsoBI CYCGRG 2 cut(s) 284, 2919
Bsp1286I GDGCHC 3 cut(s) 1124, 2066, 2416
Bsp1407I TGTACA 2 cut(s) 2236, 2788
BspANI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2571, 3354
BspCNI CTCAG 3 cut(s) 742, 2275, 3298
BspFNI CGCG 1 cut(s) 1152
BspHI TCATGA 3 cut(s) 417, 1983, 3046
BspLI GGNNCC 3 cut(s) 558, 946, 3303
BspMAI CTGCAG 3 cut(s) 1450, 1599, 3352
BspMI ACCTGC 1 cut(s) 748
BspPI GGATC 5 cut(s) 1164, 1308, 2434, 3296, 3309
BsrDI GCAATG 1 cut(s) 1391
BsrGI TGTACA 2 cut(s) 2236, 2788
BsrI ACTGG 9 cut(s) 663, 732, 869, 1655, 1673, 1829, 2471, 3103, 3114
BssECI CCNNGG 5 cut(s) 39, 552, 2067, 2686, 2991
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 3 cut(s) 552, 2067, 2991
Bst2UI CCWGG 5 cut(s) 675, 960, 2314, 2846, 3198
Bst4CI ACNGT 7 cut(s) 131, 1546, 1801, 1870, 1972, 2284, 2518
Bst6I CTCTTC 6 cut(s) 429, 1317, 2496, 2865, 3054, 3203
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 3 cut(s) 75, 611, 3230
BstAUI TGTACA 2 cut(s) 2236, 2788
BstC8I GCNNGC 3 cut(s) 71, 434, 3352
BstDEI CTNAG 4 cut(s) 750, 2144, 2262, 3306
BstDSI CCRYGG 1 cut(s) 2686
BstENI CCTNNNNNAGG 1 cut(s) 214
BstF5I GGATG 9 cut(s) 21, 295, 448, 1066, 1252, 1429, 1865, 2062, 3043
BstFNI CGCG 1 cut(s) 1152
BstH2I RGCGCY 3 cut(s) 1475, 1744, 1791
BstHHI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
BstMCI CGRYCG 2 cut(s) 27, 313
BstNI CCWGG 5 cut(s) 675, 960, 2314, 2846, 3198
BstNSI RCATGY 5 cut(s) 73, 1487, 1750, 2237, 3256
BstSCI CCNGG 5 cut(s) 673, 958, 2312, 2844, 3196
BstSFI CTRYAG 6 cut(s) 1069, 1446, 1595, 1800, 2514, 3348
BstUI CGCG 1 cut(s) 1152
BstV2I GAAGAC 3 cut(s) 160, 1442, 1547
BstX2I RGATCY 4 cut(s) 1300, 1759, 2426, 3301
BstYI RGATCY 4 cut(s) 1300, 1759, 2426, 3301
BstZI CGGCCG 1 cut(s) 24
BsuI GTATCC 2 cut(s) 299, 899
BsuRI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2571, 3354
BtgI CCRYGG 1 cut(s) 2686
BtrI CACGTC 2 cut(s) 31, 1709
BtsCI GGATG 9 cut(s) 21, 295, 448, 1066, 1252, 1429, 1865, 2062, 3043
BtsI GCAGTG 3 cut(s) 1687, 3107, 3141
BtsIMutI CAGTG 7 cut(s) 1648, 1687, 1708, 1836, 3107, 3121, 3141
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 3 cut(s) 71, 434, 3352
CaiI CAGNNNCTG 1 cut(s) 3140
CciI TCATGA 3 cut(s) 417, 1983, 3046
CfoI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
Cfr13I GGNCC 5 cut(s) 737, 944, 2138, 2398, 3149
CseI GACGC 2 cut(s) 127, 3269
CsiI ACCWGGT 1 cut(s) 2844
Csp6I GTAC 5 cut(s) 1797, 2216, 2237, 2789, 3297
CviQI GTAC 5 cut(s) 1797, 2216, 2237, 2789, 3297
DdeI CTNAG 4 cut(s) 750, 2144, 2262, 3306
DraI TTTAAA 2 cut(s) 1570, 1848
DraIII CACNNNGTG 1 cut(s) 2213
EaeI YGGCCR 3 cut(s) 24, 33, 970
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 6 cut(s) 429, 1317, 2496, 2865, 3054, 3203
EarI CTCTTC 6 cut(s) 429, 1317, 2496, 2865, 3054, 3203
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 3 cut(s) 1122, 2064, 2414
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 3 cut(s) 552, 2067, 2991
Eco147I AGGCCT 2 cut(s) 1819, 3354
Eco24I GRGCYC 3 cut(s) 1124, 2066, 2416
Eco47I GGWCC 4 cut(s) 737, 944, 2398, 3149
Eco47III AGCGCT 2 cut(s) 1473, 1742
Eco52I CGGCCG 1 cut(s) 24
Eco53kI GAGCTC 3 cut(s) 1122, 2064, 2414
Eco57I CTGAAG 4 cut(s) 609, 921, 2604, 2889
Eco88I CYCGRG 2 cut(s) 284, 2919
EcoICRI GAGCTC 3 cut(s) 1122, 2064, 2414
EcoNI CCTNNNNNAGG 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 2398
EcoRI GAATTC 1 cut(s) 986
EcoRII CCWGG 5 cut(s) 673, 958, 2312, 2844, 3196
EcoT14I CCWWGG 3 cut(s) 552, 2067, 2991
EcoT22I ATGCAT 2 cut(s) 607, 1054
EcoT38I GRGCYC 3 cut(s) 1124, 2066, 2416
ErhI CCWWGG 3 cut(s) 552, 2067, 2991
FalI AAGNNNNNCTT 4 cut(s) 155, 187, 1988, 2020
FaqI GGGAC 5 cut(s) 233, 734, 930, 2128, 2902
FauNDI CATATG 2 cut(s) 1176, 1635
FbaI TGATCA 3 cut(s) 2335, 2731, 2977
FblI GTMKAC 1 cut(s) 87
FokI GGATG 9 cut(s) 28, 302, 455, 1073, 1259, 1436, 1872, 2069, 3050
FriOI GRGCYC 3 cut(s) 1124, 2066, 2416
FspBI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2771, 3014, 3270
FspI TGCGCA 2 cut(s) 378, 1640
GlaI GCGC 8 cut(s) 74, 79, 378, 615, 1473, 1640, 1742, 1789
GsaI CCCAGC 1 cut(s) 305
GsuI CTGGAG 1 cut(s) 2454
HaeII RGCGCY 3 cut(s) 1475, 1744, 1791
HaeIII GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2571, 3354
HapII CCGG 4 cut(s) 150, 885, 1350, 3257
HgaI GACGC 2 cut(s) 127, 3269
HhaI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
Hin1I GRCGYC 1 cut(s) 138
Hin6I GCGC 8 cut(s) 73, 78, 377, 614, 1472, 1639, 1741, 1788
HinP1I GCGC 8 cut(s) 73, 78, 377, 614, 1472, 1639, 1741, 1788
HincII GTYRAC 2 cut(s) 1195, 2353
HindII GTYRAC 2 cut(s) 1195, 2353
HindIII AAGCTT 3 cut(s) 119, 1364, 2529
HpaII CCGG 4 cut(s) 150, 885, 1350, 3257
Hpy99I CGWCG 2 cut(s) 32, 659
HpyAV CCTTC 9 cut(s) 157, 273, 584, 896, 2018, 2105, 2745, 2896, 3061
HpyCH4III ACNGT 7 cut(s) 131, 1546, 1801, 1870, 1972, 2284, 2518
HpyCH4IV ACGT 3 cut(s) 30, 1708, 2355
HpyF3I CTNAG 4 cut(s) 750, 2144, 2262, 3306
HpySE526I ACGT 3 cut(s) 30, 1708, 2355
Hsp92I GRCGYC 1 cut(s) 138
HspAI GCGC 8 cut(s) 73, 78, 377, 614, 1472, 1639, 1741, 1788
Ksp22I TGATCA 3 cut(s) 2335, 2731, 2977
LmnI GCTCC 3 cut(s) 367, 1954, 2069
MabI ACCWGGT 1 cut(s) 2844
MaeI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2771, 3014, 3270
MaeII ACGT 3 cut(s) 30, 1708, 2355
MaeIII GTNAC 6 cut(s) 139, 468, 1546, 1903, 2380, 3022
MfeI CAATTG 2 cut(s) 3027, 3362
MflI RGATCY 4 cut(s) 1300, 1759, 2426, 3301
MhlI GDGCHC 3 cut(s) 1124, 2066, 2416
MlsI TGGCCA 1 cut(s) 972
MluNI TGGCCA 1 cut(s) 972
MlyI GAGTC 8 cut(s) 155, 415, 668, 1093, 1347, 1582, 2294, 2813
MmeI TCCRAC 2 cut(s) 1954, 2034
Mox20I TGGCCA 1 cut(s) 972
Mph1103I ATGCAT 2 cut(s) 607, 1054
MroXI GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
MscI TGGCCA 1 cut(s) 972
MseI TTAA 9 cut(s) 1140, 1181, 1206, 1569, 1665, 1847, 2445, 2592, 3077
MslI CAYNNNNRTG 5 cut(s) 441, 914, 1488, 2964, 2985
Msp20I TGGCCA 1 cut(s) 972
MspA1I CMGCKG 2 cut(s) 301, 3347
MspI CCGG 4 cut(s) 150, 885, 1350, 3257
MspR9I CCNGG 5 cut(s) 675, 960, 2314, 2846, 3198
MteI GCGCNGCGC 1 cut(s) 76
MunI CAATTG 2 cut(s) 3027, 3362
MvaI CCWGG 5 cut(s) 675, 960, 2314, 2846, 3198
MvnI CGCG 1 cut(s) 1152
NdeI CATATG 2 cut(s) 1176, 1635
NlaIV GGNNCC 3 cut(s) 558, 946, 3303
NmeAIII GCCGAG 2 cut(s) 64, 1474
NmuCI GTSAC 5 cut(s) 139, 468, 1903, 2380, 3022
NsbI TGCGCA 2 cut(s) 378, 1640
NsiI ATGCAT 2 cut(s) 607, 1054
NspI RCATGY 5 cut(s) 73, 1487, 1750, 2237, 3256
PaeI GCATGC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 3 cut(s) 417, 1983, 3046
PceI AGGCCT 2 cut(s) 1819, 3354
PciI ACATGT 2 cut(s) 1746, 2233
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 2352
PdmI GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
Ple19I CGATCG 1 cut(s) 313
PleI GAGTC 8 cut(s) 154, 415, 667, 1092, 1347, 1582, 2294, 2812
PpsI GAGTC 8 cut(s) 154, 415, 667, 1092, 1347, 1582, 2294, 2812
PpuMI RGGWCCY 1 cut(s) 2398
PscI ACATGT 2 cut(s) 1746, 2233
PsiI TTATAA 3 cut(s) 2454, 2892, 3156
Psp124BI GAGCTC 3 cut(s) 1124, 2066, 2416
Psp5II RGGWCCY 1 cut(s) 2398
Psp6I CCWGG 5 cut(s) 673, 958, 2312, 2844, 3196
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 5 cut(s) 673, 958, 2312, 2844, 3196
PspN4I GGNNCC 3 cut(s) 558, 946, 3303
PspPI GGNCC 5 cut(s) 737, 944, 2138, 2398, 3149
PspPPI RGGWCCY 1 cut(s) 2398
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 3 cut(s) 1450, 1599, 3352
PstNI CAGNNNCTG 1 cut(s) 3140
PsuI RGATCY 4 cut(s) 1300, 1759, 2426, 3301
PvuI CGATCG 1 cut(s) 313
PvuII CAGCTG 2 cut(s) 301, 3347
RsaI GTAC 5 cut(s) 1798, 2217, 2238, 2790, 3298
RsaNI GTAC 5 cut(s) 1797, 2216, 2237, 2789, 3297
RseI CAYNNNNRTG 5 cut(s) 441, 914, 1488, 2964, 2985
SacI GAGCTC 3 cut(s) 1124, 2066, 2416
SaqAI TTAA 9 cut(s) 1140, 1181, 1206, 1569, 1665, 1847, 2445, 2592, 3077
Sau96I GGNCC 5 cut(s) 737, 944, 2138, 2398, 3149
ScaI AGTACT 1 cut(s) 1798
SchI GAGTC 8 cut(s) 155, 415, 668, 1093, 1347, 1582, 2294, 2813
ScrFI CCNGG 5 cut(s) 675, 960, 2314, 2846, 3198
SduI GDGCHC 3 cut(s) 1124, 2066, 2416
SexAI ACCWGGT 1 cut(s) 2844
SfcI CTRYAG 6 cut(s) 1069, 1446, 1595, 1800, 2514, 3348
SfiI GGCCNNNNNGGCC 1 cut(s) 32
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 4 cut(s) 737, 944, 2398, 3149
SlaI CTCGAG 1 cut(s) 284
SmiMI CAYNNNNRTG 5 cut(s) 441, 914, 1488, 2964, 2985
SmlI CTYRAG 6 cut(s) 52, 284, 2393, 2561, 2705, 2826
SmoI CTYRAG 6 cut(s) 52, 284, 2393, 2561, 2705, 2826
SpeI ACTAGT 1 cut(s) 1907
SphI GCATGC 1 cut(s) 73
SseBI AGGCCT 2 cut(s) 1819, 3354
SspI AATATT 1 cut(s) 388
SspMI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2771, 3014, 3270
SstI GAGCTC 3 cut(s) 1124, 2066, 2416
StuI AGGCCT 2 cut(s) 1819, 3354
StyD4I CCNGG 5 cut(s) 673, 958, 2312, 2844, 3196
StyI CCWWGG 3 cut(s) 552, 2067, 2991
TaaI ACNGT 7 cut(s) 131, 1546, 1801, 1870, 1972, 2284, 2518
TaiI ACGT 3 cut(s) 33, 1711, 2358
TaqI TCGA 9 cut(s) 180, 285, 499, 595, 657, 840, 1019, 2540, 3181
TatI WGTACW 3 cut(s) 1796, 2236, 2788
TauI GCSGC 5 cut(s) 38, 61, 138, 1886, 3320
Tru1I TTAA 9 cut(s) 1140, 1181, 1206, 1569, 1665, 1847, 2445, 2592, 3077
Tru9I TTAA 9 cut(s) 1140, 1181, 1206, 1569, 1665, 1847, 2445, 2592, 3077
TscAI CASTG 7 cut(s) 1655, 1687, 1708, 1836, 3114, 3121, 3141
TseFI GTSAC 5 cut(s) 139, 468, 1903, 2380, 3022
Tsp45I GTSAC 5 cut(s) 139, 468, 1903, 2380, 3022
TspGWI ACGGA 4 cut(s) 225, 738, 2621, 3314
TspRI CASTG 7 cut(s) 1655, 1687, 1708, 1836, 3114, 3121, 3141
VpaK11BI GGWCC 4 cut(s) 737, 944, 2398, 3149
XagI CCTNNNNNAGG 1 cut(s) 214
XapI RAATTY 6 cut(s) 854, 928, 986, 1661, 2193, 2305
XceI RCATGY 5 cut(s) 73, 1487, 1750, 2237, 3256
XhoI CTCGAG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
XspI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2771, 3014, 3270
ZrmI AGTACT 1 cut(s) 1798
Zsp2I ATGCAT 2 cut(s) 607, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.