MD13G1148000.v1.1

Nuclear export mediator factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013357
Physical Location & Seq
Reverse (-)
11560277 .. 11567236
6960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1148000.v1.1.491

Sequence Viewer

Length: 3489 bp
ATGGTGAAGGTCCGGATGAACACCGCCGATGTCGCCGCCGAGGTCAAGTGCTTGCGGCGGCTTATCGGCATGCGCTGCTCCAACGTCTACGATCTCTCCCCCAAGACGTATATGTTGAAGCTCATGAATAGCAGTGGAGTCACAGAGTCCGGCGAGAGCGAGAAGGTTTTTCTATTGATTGAGAGTGGCGTCAGATTGCATACGACTCAATACGTTCGGGATAAGAGTAATACTCCATCTGGGTTTACCTTAAAATTGAGGAAGCACATACGAACACGAAGGCTCGAGGATGTACGGCAGCTTGGATATGATAGGATTGTTCTCTTTCAATTTGGGCTCGGTGAAAATGCGTACTATGTTATATTGGAGCTTTATGCCCAGGGAAATGTTATTCTCGCAGATTCGGATTTTATGGTCATGACCCTTCTTCGCTCCCATAGGGATGACGATAAAGGGGTTGCAATCATGTCACGTCATCGTTATCCAATTGAAATATGTCGAGTTTTTGAGCGAACAACTGCTGAAAGGTTACAGGAAGCTCTTACCTTGTCTAAGGAACCAGATAACAATGAACCCGTTAAGGATCATGAAGGTGGAAATAATGTGCCTGATGCACCAAAAGAGAAAAAGGGTAAAAGTAAAGGTGGGAAGCCTGCTGAGTCAAGTAAAAGTAGCGGTGATACTAAAGCAAAACAGGCCACCTTGAAGAATGTTCTTGGGGATGCGTTGGGCTATGGACCTGCGCTATCTGAGCATATTATATTAGATGCTGGCCTCATTCCAAATACAAAAGTTTCTAAAGAGAACAAGTTAGACGATAATGCAATTCAGCTTTTGGTTGAAGCTGTTGCGAAGTTTGAGGATTGGCTGCATGATGTCATTTCAGGTGATAAAATTCCTGAAGGGTACATTTTAATGCAGAAGAATTCAGGGAAAAGTGCTCCCCCATCTGAACCAGGAAGTTCTGTCCAGATATATGATGAATTCTGCCCCATATTATTGAACCAATTCAAATCGAGGGAGCATGTGGAGTTAGAAACATTTGATGCTTCTTTGGATGAGTTCTATAGCAAAATTGAGAGTCAAAGGGCAGAACAACAGCAAAAGGCGAAAGAGAGCTCTGCCACCCAGAAGCTTAATAAAATACGTGTTGATCAGGAAAATCGTGTTCACATGCTAAGGAAAGAAGTTGATCAGTGTGTTAAAATGGCGGAATTGATAGAATATAATTTAGATGATGTGGATGCTGCTATAGTAGCTGTTCGTGTAGCTTTAGCAAAGGGCACAAGTTGGGAGGATATTGCTAGAATGGTGAAGGAGGAGAAGAAACATGGAAACCCTGTTGCTAGCCTCATTGACAAGCTTCAGCTTGAAAAGAATTGCATGACATTGCTGCTGAGCAACAATCTTGATGAAATGGATGATGATGAGAAGACTCTCCCTGCAGATAAGGTGGAAGTTGATTTAGCACTTTCAGCACATGCCAATGCTCGGCGGTGGTATGAACTAAAGAAAAAACAGGAGAGCAAACAGGAGAAGACTGTAACAGCACATGAAAAAGCATTTAAGGCGGCGGAGAAAAAGACTCGTCTCCAGCTGTCACAGGAAAAAGCTGTTGCTAGCATTTCACATATGCGCAAGGTTCACTGGTTTGAGAAATTTAACTGGTTCATCAGCAGTGAGAACTATTTGATTATCAGTGGACGTGATGCTCAACAAAATGAGATGATAGTCAAGCGCTATATGTCAAAAGGAGATCTGTATGTTCATGCGGAGTTGCATGGGGCTTCCAGCACCGTGATAAAGAACCATAGGCCTGACCAACCAGTGCCTCCACTTACATTGAACCAAGCAGGATGCTTCACAGTTTGCCACAGTGCAGCATGGGATTCAAAGATTGTCACTAGTGCGTGGTGGGTTCATCCTCACCAGGTCAGTAAAACTGCTCCTACTGGGGAGTATCTGACAGTTGGAAGTTTTATGATTCGTGGGAAGAAGAATTTTCTTCCTCCACACCCTCTAATTATGGGCTTTGGGTTGTTATTTCGCTTGGACGAGAGCTCATTGGGCTCCCATCTGAATGAGAGGAGGGTAAGAGGTGAGGAGGAAGGGACGAATGATGTTGATGAAAATGGACCTCTAGAAGAGGTATCTGATTCTGAGTCAGAGAATGAAACCGTAGAGGAAAAACTTGCAGAAGAACCAAAACCTGCTCCTGATTCATCTATACATATTGATAAACCAGATCTGAAAGACCCATCTGGGAATGGCTTACTTACAAGCAATGCTGAAGCAAAAGACTCGGCTGAAATTCCCACGAAAGAAAAGAAGACCTTGAGTGATGCTGATATTTCTGTAAATGGTGTTTCATCTGTCACCCCAGAACTTGAGGATCTCATTGATATAGCTCTTGGGCTTGGATCTACAGCTATGTCTGCTAAAAAATATCAAATTGACACTTCTCCCGTTGATTTGGTAGCAGAACCTAATGTTGAGGAGAATAAGGCTACAGGAAGAGAGAAACCTCATATTTCAAGAGCTGAAAGAAGAAAGCTCAAGAAAGGACAGACTGGTTGTGCTCATGAAGAACAAGCTGATCTGCAAAACGAAAAATTGAAACAACATGAGAGTTCAGTCAGTCAGCCAGAAGAGGAAGTTCATGAGAAGAAGCCTAGTGGTGGAAAAACCAGCCGATCAAAACAACACAATATTTCTGTCAGACAGACCGAAAATGAAGTTCATGATAAGAAGCCAAGTAGTGGAAAAGCCAGCCGTGGACAAAAGGGTAAACTCAAGAAGATGAAAGAGAAGTATGCCGACCAGGATGAGGAAGAAAGAAGGATCCGTATGGCTTTGCTAGCTTCTGCTGGAAGAGTACAGAAGAGTGGAGAGTCCCAAAATGAAAATTCAGCTCTGGCTGAAGATAAGAAACTGAGTCCTGTCGATGCTCCAAAAATATGTTATAAATGTAAAAAGGTAGGTCATCTGTCCCGGGATTGTCCAGAACATCAAGATGGTACGTTACATAGTCATGCAAATGGAGTTGAAGATGACCCTCCTGTGGGTTTGGATAAATCTACTTCTGAAGTTGACAAGGTGACAATGGAGGAGGATGATATTCATGAGATAGGTGAAGAGGAGAAGGAGAAATTAAACGATGTCGATTACTTGACGGGCAATCCACTGCCCAGTGATATTCTCTTATATGCGGTGCCTGTCTGTGGTCCTTATAGCTCCGTCCAGTCTTATAAATATCATGTGAAGATAGTTCCAGGCTCTGTGAAGAGGGGGAAAGCTGCGAAAACTGCCATGAATTTGTTTAGCCACAGGCCGGAAGCAACGGTTAGAGAGAAAGAGTTGATGAAAGCATGTACTGATCCTGAGCTGGTTGCTGCAATTATCGGCAATGTGAAGATAACATCTGCAGGCCTCACTCAGTTGAAGCAGAAGCAGAAGAAAGTCAAGAAGAGCAGCAGCAAAGCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006913 GO:0008150 GO:0046907 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3441 DUF814 FbpA zf-CCHC

Protein Analysis

1163

Amino Acids

129.6

Weight (kDa)

6.46

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT_N PF05833 18 - 149 5.9e-14 NFACT N-terminal and middle domains
NFACT_N PF05833 230 - 538 3.2e-08 NFACT N-terminal and middle domains
NFACT-R_1 PF05670 553 - 664 2.2e-40 NFACT protein RNA binding domain
zf-CCHC PF00098 986 - 1002 2.6e-06 Zinc knuckle
NFACT-C PF11923 1047 - 1140 1e-26 NFACT protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013357)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49930
fragaria_vesca FvH4_4g20770 FvH4_4g20770 FvH4_4g20770
malus_domestica MD13G1148000.v1.1 MD16G1147800.v1.1
prunus_persica Prupe.1G111500_v2.0.a1
pyrus_communis pycom13g12740 pycom16g12630
rosa_chinensis RchiOBHm_Chr4g0426211
rosa_laevigata RLG00000007310
rosa_roxburghii Rroxscaffold_5G00368230
rosa_rugosa Rorug04G0209600
rosa_samantha Rh4AG266500 Rh4BG272500 Rh4CG285700 Rh4DG268800
rosa_wichuraiana Rw4G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2964, 3249
Acc16I TGCGCA 1 cut(s) 1637
Acc36I ACCTGC 2 cut(s) 748, 2218
AccB1I GGYRCC 1 cut(s) 3211
AccB7I CCANNNNNTGG 1 cut(s) 2759
AccI GTMKAC 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 12
AclWI GGATC 6 cut(s) 591, 2400, 2428, 2836, 2849, 3371
AcsI RAATTY 8 cut(s) 894, 925, 983, 1658, 1999, 2310, 2905, 3313
AcuI CTGAAG 5 cut(s) 921, 1349, 2310, 2940, 3105
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 6 cut(s) 294, 353, 908, 2877, 3019, 3373
AfeI AGCGCT 1 cut(s) 1739
AfiI CCNNNNNNNGG 8 cut(s) 1491, 2678, 2759, 2827, 3061, 3062, 3221, 3331
AflIII ACRYGT 1 cut(s) 1147
AhlI ACTAGT 1 cut(s) 1904
AjiI CACGTC 2 cut(s) 473, 1706
AjnI CCWGG 5 cut(s) 378, 955, 1929, 2820, 3271
Alw21I GWGCWC 4 cut(s) 943, 1121, 2063, 2581
Alw26I GTCTC 1 cut(s) 1595
AlwI GGATC 6 cut(s) 591, 2400, 2428, 2836, 2849, 3371
AlwNI CAGNNNCTG 1 cut(s) 3278
Ama87I CYCGRG 2 cut(s) 284, 2991
Aor13HI TCCGGA 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 1739
AoxI GGCC 5 cut(s) 696, 772, 1814, 3329, 3427
ApoI RAATTY 8 cut(s) 894, 925, 983, 1658, 1999, 2310, 2905, 3313
Asp700I GAANNNNTTC 1 cut(s) 1007
AspLEI GCGC 4 cut(s) 75, 745, 1638, 1740
AspS9I GGNCC 4 cut(s) 10, 737, 2135, 3224
AsuC2I CCSGG 2 cut(s) 2992, 2993
AsuNHI GCTAGC 3 cut(s) 1346, 1619, 2857
AvaI CYCGRG 2 cut(s) 284, 2991
AvaII GGWCC 4 cut(s) 10, 737, 2135, 3224
BaeGI GKGCMC 1 cut(s) 1286
BamHI GGATCC 1 cut(s) 2841
BanI GGYRCC 1 cut(s) 3211
BanII GRGCYC 4 cut(s) 339, 1121, 2063, 2072
BbsI GAAGAC 3 cut(s) 1439, 1544, 2336
Bbv12I GWGCWC 4 cut(s) 943, 1121, 2063, 2581
BbvI GCAGC 9 cut(s) 62, 310, 855, 1234, 1380, 1892, 3283, 3379, 3483
BccI CCATC 5 cut(s) 244, 955, 2082, 2266, 3008
BceAI ACGGC 2 cut(s) 311, 2757
BcgI CGANNNNNNTGC 2 cut(s) 2283, 2317
BciT130I CCWGG 5 cut(s) 380, 957, 1931, 2822, 3273
BclI TGATCA 2 cut(s) 1153, 1192
BcnI CCSGG 2 cut(s) 2992, 2993
BcoDI GTCTC 1 cut(s) 1595
BcuI ACTAGT 1 cut(s) 1904
BfaI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
BfmI CTRYAG 6 cut(s) 1066, 1251, 1443, 2424, 2508, 3423
BfoI RGCGCY 1 cut(s) 1741
BfuAI ACCTGC 2 cut(s) 748, 2218
BglII AGATCT 2 cut(s) 1756, 2245
BlpI GCTNAGC 1 cut(s) 1397
Bme1390I CCNGG 7 cut(s) 380, 957, 1931, 2822, 2992, 2993, 3273
Bme18I GGWCC 4 cut(s) 10, 737, 2135, 3224
BmeT110I CYCGRG 2 cut(s) 284, 2991
BmgBI CACGTC 2 cut(s) 473, 1706
BmgT120I GGNCC 4 cut(s) 10, 737, 2135, 3224
BmiI GGNNCC 4 cut(s) 558, 2071, 2843, 3213
BmrFI CCNGG 7 cut(s) 380, 957, 1931, 2822, 2992, 2993, 3273
BmrI ACTGGG 2 cut(s) 1962, 3183
BmsI GCATC 9 cut(s) 601, 712, 757, 1036, 1234, 1699, 1847, 2332, 2935
BmtI GCTAGC 3 cut(s) 1350, 1623, 2861
BmuI ACTGGG 2 cut(s) 1962, 3183
BpiI GAAGAC 3 cut(s) 1439, 1544, 2336
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpmI CTGGAG 1 cut(s) 1577
Bpu10I CCTNAGC 2 cut(s) 1178, 3381
Bpu1102I GCTNAGC 1 cut(s) 1397
BpuEI CTTGAG 4 cut(s) 2356, 2408, 2540, 2777
BpuMI CCSGG 2 cut(s) 2992, 2993
BsaAI YACGTR 1 cut(s) 1148
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 5 cut(s) 39, 378, 379, 2773, 2991
BsaWI WCCGGW 1 cut(s) 12
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 8 cut(s) 1491, 2678, 2759, 2827, 3061, 3062, 3221, 3331
Bse1I ACTGG 7 cut(s) 1652, 1670, 1826, 1957, 2575, 3189, 3241
Bse3DI GCAATG 3 cut(s) 1388, 2290, 3412
BseAI TCCGGA 1 cut(s) 12
BseBI CCWGG 5 cut(s) 380, 957, 1931, 2822, 3273
BseDI CCNNGG 5 cut(s) 39, 378, 379, 2773, 2991
BseLI CCNNNNNNNGG 8 cut(s) 1491, 2678, 2759, 2827, 3061, 3062, 3221, 3331
BseMI GCAATG 3 cut(s) 1388, 2290, 3412
BseMII CTCAG 7 cut(s) 648, 741, 1388, 2151, 2924, 3372, 3449
BseNI ACTGG 7 cut(s) 1652, 1670, 1826, 1957, 2575, 3189, 3241
BseRI GAGGAG 6 cut(s) 1334, 2101, 2117, 2510, 3122, 3152
BseSI GKGCMC 1 cut(s) 1286
BseXI GCAGC 9 cut(s) 62, 310, 855, 1234, 1380, 1892, 3283, 3379, 3483
BsgI GTGCAG 1 cut(s) 1899
BshFI GGCC 5 cut(s) 698, 774, 1816, 3331, 3429
BshNI GGYRCC 1 cut(s) 3211
BsiHKAI GWGCWC 4 cut(s) 943, 1121, 2063, 2581
BsiHKCI CYCGRG 2 cut(s) 284, 2991
BsiSI CCGG 4 cut(s) 13, 150, 2992, 3332
BslFI GGGAC 3 cut(s) 2125, 2878, 2974
BslI CCNNNNNNNGG 8 cut(s) 1491, 2678, 2759, 2827, 3061, 3062, 3221, 3331
BsmAI GTCTC 1 cut(s) 1595
BsmBI CGTCTC 1 cut(s) 1595
BsmFI GGGAC 3 cut(s) 2125, 2878, 2974
BsnI GGCC 5 cut(s) 698, 774, 1816, 3331, 3429
BsoBI CYCGRG 2 cut(s) 284, 2991
Bsp1286I GDGCHC 7 cut(s) 339, 943, 1121, 1286, 2063, 2072, 2581
Bsp13I TCCGGA 1 cut(s) 12
Bsp1720I GCTNAGC 1 cut(s) 1397
BspANI GGCC 5 cut(s) 698, 774, 1816, 3331, 3429
BspCNI CTCAG 7 cut(s) 649, 742, 1389, 2152, 2925, 3373, 3448
BspEI TCCGGA 1 cut(s) 12
BspHI TCATGA 7 cut(s) 123, 417, 586, 2581, 2659, 2740, 3121
BspLI GGNNCC 4 cut(s) 558, 2071, 2843, 3213
BspMAI CTGCAG 2 cut(s) 1447, 3427
BspMI ACCTGC 2 cut(s) 748, 2218
BspOI GCTAGC 3 cut(s) 1350, 1623, 2861
BspPI GGATC 6 cut(s) 591, 2400, 2428, 2836, 2849, 3371
BspQI GCTCTTC 1 cut(s) 3461
BspT107I GGYRCC 1 cut(s) 3211
BsrDI GCAATG 3 cut(s) 1388, 2290, 3412
BsrI ACTGG 7 cut(s) 1652, 1670, 1826, 1957, 2575, 3189, 3241
BssECI CCNNGG 5 cut(s) 39, 378, 379, 2773, 2991
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 5 cut(s) 380, 957, 1931, 2822, 3273
Bst4CI ACNGT 7 cut(s) 1543, 1798, 1867, 1877, 1969, 2179, 3343
Bst6I CTCTTC 8 cut(s) 2139, 2509, 2643, 2866, 2876, 3129, 3278, 3461
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 1148
BstC8I GCNNGC 9 cut(s) 53, 71, 654, 772, 1348, 1621, 2770, 2859, 3427
BstDSI CCRYGG 1 cut(s) 2773
BstH2I RGCGCY 1 cut(s) 1741
BstHHI GCGC 4 cut(s) 75, 745, 1638, 1740
BstMAI GTCTC 1 cut(s) 1595
BstNI CCWGG 5 cut(s) 380, 957, 1931, 2822, 3273
BstNSI RCATGY 5 cut(s) 73, 1028, 1177, 1484, 3372
BstSCI CCNGG 7 cut(s) 378, 955, 1929, 2820, 2990, 2991, 3271
BstSFI CTRYAG 6 cut(s) 1066, 1251, 1443, 2424, 2508, 3423
BstSLI GKGCMC 1 cut(s) 1286
BstV1I GCAGC 9 cut(s) 62, 310, 855, 1234, 1380, 1892, 3283, 3379, 3483
BstV2I GAAGAC 3 cut(s) 1439, 1544, 2336
BstX2I RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
BstYI RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
BsuRI GGCC 5 cut(s) 698, 774, 1816, 3331, 3429
BtgI CCRYGG 1 cut(s) 2773
BtrI CACGTC 2 cut(s) 473, 1706
BtsI GCAGTG 3 cut(s) 139, 1684, 3182
BtsIMutI CAGTG 9 cut(s) 139, 1202, 1645, 1684, 1705, 1833, 1882, 3182, 3196
BveI ACCTGC 2 cut(s) 748, 2218
Cac8I GCNNGC 9 cut(s) 53, 71, 654, 772, 1348, 1621, 2770, 2859, 3427
CaiI CAGNNNCTG 1 cut(s) 3278
CciI TCATGA 7 cut(s) 123, 417, 586, 2581, 2659, 2740, 3121
CfoI GCGC 4 cut(s) 75, 745, 1638, 1740
Cfr13I GGNCC 4 cut(s) 10, 737, 2135, 3224
Cfr9I CCCGGG 1 cut(s) 2991
CseI GACGC 1 cut(s) 178
CsiI ACCWGGT 1 cut(s) 1929
Csp6I GTAC 6 cut(s) 293, 352, 907, 2876, 3018, 3372
CspCI CAANNNNNGTGG 2 cut(s) 1824, 1859
CviQI GTAC 6 cut(s) 293, 352, 907, 2876, 3018, 3372
Eam1104I CTCTTC 8 cut(s) 2139, 2509, 2643, 2866, 2876, 3129, 3278, 3461
EarI CTCTTC 8 cut(s) 2139, 2509, 2643, 2866, 2876, 3129, 3278, 3461
EciI GGCGGA 2 cut(s) 1226, 1589
Ecl136II GAGCTC 2 cut(s) 1119, 2061
Eco147I AGGCCT 2 cut(s) 1816, 3429
Eco24I GRGCYC 4 cut(s) 339, 1121, 2063, 2072
Eco47I GGWCC 4 cut(s) 10, 737, 2135, 3224
Eco47III AGCGCT 1 cut(s) 1739
Eco53kI GAGCTC 2 cut(s) 1119, 2061
Eco57I CTGAAG 5 cut(s) 921, 1349, 2310, 2940, 3105
Eco88I CYCGRG 2 cut(s) 284, 2991
EcoICRI GAGCTC 2 cut(s) 1119, 2061
EcoRI GAATTC 2 cut(s) 925, 983
EcoRII CCWGG 5 cut(s) 378, 955, 1929, 2820, 3271
EcoT38I GRGCYC 4 cut(s) 339, 1121, 2063, 2072
Esp3I CGTCTC 1 cut(s) 1595
FalI AAGNNNNNCTT 2 cut(s) 2318, 2350
FaqI GGGAC 3 cut(s) 2125, 2878, 2974
FauNDI CATATG 1 cut(s) 1632
FbaI TGATCA 2 cut(s) 1153, 1192
FblI GTMKAC 1 cut(s) 87
FriOI GRGCYC 4 cut(s) 339, 1121, 2063, 2072
FspBI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
FspI TGCGCA 1 cut(s) 1637
GlaI GCGC 4 cut(s) 74, 744, 1637, 1739
GsuI CTGGAG 1 cut(s) 1577
HaeII RGCGCY 1 cut(s) 1741
HaeIII GGCC 5 cut(s) 698, 774, 1816, 3331, 3429
HapII CCGG 4 cut(s) 13, 150, 2992, 3332
HgaI GACGC 1 cut(s) 178
HhaI GCGC 4 cut(s) 75, 745, 1638, 1740
Hin1I GRCGYC 1 cut(s) 189
Hin6I GCGC 4 cut(s) 73, 743, 1636, 1738
HinP1I GCGC 4 cut(s) 73, 743, 1636, 1738
HincII GTYRAC 1 cut(s) 3091
HindII GTYRAC 1 cut(s) 3091
HindIII AAGCTT 2 cut(s) 1133, 1361
HpaII CCGG 4 cut(s) 13, 150, 2992, 3332
Hpy166II GTNNAC 8 cut(s) 88, 246, 1171, 1645, 1703, 2777, 2789, 3091
Hpy8I GTNNAC 8 cut(s) 88, 246, 1171, 1645, 1703, 2777, 2789, 3091
HpyAV CCTTC 9 cut(s) 157, 273, 434, 584, 896, 1309, 2102, 2832, 3136
HpyCH4III ACNGT 7 cut(s) 1543, 1798, 1867, 1877, 1969, 2179, 3343
HpyCH4IV ACGT 7 cut(s) 84, 107, 213, 472, 1147, 1705, 3020
HpySE526I ACGT 7 cut(s) 84, 107, 213, 472, 1147, 1705, 3020
Hsp92I GRCGYC 1 cut(s) 189
HspAI GCGC 4 cut(s) 73, 743, 1636, 1738
Kpn2I TCCGGA 1 cut(s) 12
Ksp22I TGATCA 2 cut(s) 1153, 1192
LguI GCTCTTC 1 cut(s) 3461
Lsp1109I GCAGC 9 cut(s) 62, 310, 855, 1234, 1380, 1892, 3283, 3379, 3483
LweI GCATC 9 cut(s) 601, 712, 757, 1036, 1234, 1699, 1847, 2332, 2935
MabI ACCWGGT 1 cut(s) 1929
MaeI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
MaeII ACGT 7 cut(s) 84, 107, 213, 472, 1147, 1705, 3020
MaeIII GTNAC 9 cut(s) 139, 468, 528, 1543, 1599, 1900, 2374, 3021, 3097
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
MhlI GDGCHC 7 cut(s) 339, 943, 1121, 1286, 2063, 2072, 2581
MmeI TCCRAC 2 cut(s) 105, 1951
MroI TCCGGA 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 1007
MseI TTAA 8 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 3152
MslI CAYNNNNRTG 8 cut(s) 441, 591, 914, 1485, 2079, 3012, 3030, 3036
MspA1I CMGCKG 1 cut(s) 1597
MspI CCGG 4 cut(s) 13, 150, 2992, 3332
MspR9I CCNGG 7 cut(s) 380, 957, 1931, 2822, 2992, 2993, 3273
MunI CAATTG 1 cut(s) 486
MvaI CCWGG 5 cut(s) 380, 957, 1931, 2822, 3273
NciI CCSGG 2 cut(s) 2992, 2993
NdeI CATATG 1 cut(s) 1632
NheI GCTAGC 3 cut(s) 1346, 1619, 2857
NlaIV GGNNCC 4 cut(s) 558, 2071, 2843, 3213
NmeAIII GCCGAG 3 cut(s) 64, 1471, 2282
NmuCI GTSAC 6 cut(s) 139, 468, 1599, 1900, 2374, 3097
NsbI TGCGCA 1 cut(s) 1637
NspI RCATGY 5 cut(s) 73, 1028, 1177, 1484, 3372
PaeI GCATGC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 7 cut(s) 123, 417, 586, 2581, 2659, 2740, 3121
PasI CCCWGGG 1 cut(s) 379
PceI AGGCCT 2 cut(s) 1816, 3429
PciSI GCTCTTC 1 cut(s) 3461
PdmI GAANNNNTTC 1 cut(s) 1007
PfeI GAWTC 5 cut(s) 401, 1889, 1984, 2156, 2219
PflMI CCANNNNNTGG 1 cut(s) 2759
Ppu21I YACGTR 1 cut(s) 1148
PsiI TTATAA 2 cut(s) 2964, 3249
Psp124BI GAGCTC 2 cut(s) 1121, 2063
Psp6I CCWGG 5 cut(s) 378, 955, 1929, 2820, 3271
PspGI CCWGG 5 cut(s) 378, 955, 1929, 2820, 3271
PspN4I GGNNCC 4 cut(s) 558, 2071, 2843, 3213
PspPI GGNCC 4 cut(s) 10, 737, 2135, 3224
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 2 cut(s) 1447, 3427
PstNI CAGNNNCTG 1 cut(s) 3278
PsuI RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
PvuII CAGCTG 1 cut(s) 1597
RsaI GTAC 6 cut(s) 294, 353, 908, 2877, 3019, 3373
RsaNI GTAC 6 cut(s) 293, 352, 907, 2876, 3018, 3372
RseI CAYNNNNRTG 8 cut(s) 441, 591, 914, 1485, 2079, 3012, 3030, 3036
SacI GAGCTC 2 cut(s) 1121, 2063
SapI GCTCTTC 1 cut(s) 3461
SaqAI TTAA 8 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 3152
Sau96I GGNCC 4 cut(s) 10, 737, 2135, 3224
ScrFI CCNGG 7 cut(s) 380, 957, 1931, 2822, 2992, 2993, 3273
SduI GDGCHC 7 cut(s) 339, 943, 1121, 1286, 2063, 2072, 2581
SexAI ACCWGGT 1 cut(s) 1929
SfaNI GCATC 9 cut(s) 601, 712, 757, 1036, 1234, 1699, 1847, 2332, 2935
SfcI CTRYAG 6 cut(s) 1066, 1251, 1443, 2424, 2508, 3423
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 4 cut(s) 10, 737, 2135, 3224
SlaI CTCGAG 1 cut(s) 284
SmaI CCCGGG 1 cut(s) 2993
SmiMI CAYNNNNRTG 8 cut(s) 441, 591, 914, 1485, 2079, 3012, 3030, 3036
SmlI CTYRAG 5 cut(s) 284, 2335, 2387, 2555, 2792
SmoI CTYRAG 5 cut(s) 284, 2335, 2387, 2555, 2792
SpeI ACTAGT 1 cut(s) 1904
SphI GCATGC 1 cut(s) 73
SseBI AGGCCT 2 cut(s) 1816, 3429
SspI AATATT 1 cut(s) 2710
SspMI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
SstI GAGCTC 2 cut(s) 1121, 2063
StuI AGGCCT 2 cut(s) 1816, 3429
StyD4I CCNGG 7 cut(s) 378, 955, 1929, 2820, 2990, 2991, 3271
TaaI ACNGT 7 cut(s) 1543, 1798, 1867, 1877, 1969, 2179, 3343
TaiI ACGT 7 cut(s) 87, 110, 216, 475, 1150, 1708, 3023
TaqI TCGA 5 cut(s) 285, 499, 1016, 2943, 3162
TaqII GACCGA 1 cut(s) 2741
TatI WGTACW 2 cut(s) 2875, 3371
TauI GCSGC 4 cut(s) 38, 58, 61, 1574
TfiI GAWTC 5 cut(s) 401, 1889, 1984, 2156, 2219
Tru1I TTAA 8 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 3152
Tru9I TTAA 8 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 3152
TscAI CASTG 9 cut(s) 139, 1202, 1652, 1684, 1705, 1833, 1882, 3189, 3196
TseFI GTSAC 6 cut(s) 139, 468, 1599, 1900, 2374, 3097
Tsp45I GTSAC 6 cut(s) 139, 468, 1599, 1900, 2374, 3097
TspGWI ACGGA 2 cut(s) 2834, 3226
TspMI CCCGGG 1 cut(s) 2991
TspRI CASTG 9 cut(s) 139, 1202, 1652, 1684, 1705, 1833, 1882, 3189, 3196
Van91I CCANNNNNTGG 1 cut(s) 2759
VpaK11BI GGWCC 4 cut(s) 10, 737, 2135, 3224
XapI RAATTY 8 cut(s) 894, 925, 983, 1658, 1999, 2310, 2905, 3313
XbaI TCTAGA 1 cut(s) 2140
XceI RCATGY 5 cut(s) 73, 1028, 1177, 1484, 3372
XhoI CTCGAG 1 cut(s) 284
XmaI CCCGGG 1 cut(s) 2991
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 1007
XspI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.