MD16G1147800.v1.1

Nuclear export mediator factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013357
Physical Location & Seq
Reverse (-)
11534435 .. 11540783
6349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1147800.v1.1.491

Sequence Viewer

Length: 3489 bp
ATGGTGAAGGTCCGGATGAACACCGCCGATGTCGCCGCCGAGGTCAAGTGCTTGCGGCGGCTCATCGGCATGCGCTGCTCCAACGTGTACGATATCTCCCCCAAGACGTATATGTTCAAGCTTATGAACAGCAGTGGAGTGACAGAGTCCGGCGAGAGCGAGAAGGTCTTTCTATTGATTGAGAGTGGCGTCAGATTGCATACAACTCAATACGTTCGTGACAAGAGTAATACTCCATCTGGGTTTACCCTAAAATTGAGGAAGCACATACGAACACGAAGGCTCGAGGATGTACGCCAGCTTGGATATGATAGGATTGTTCTCTTTCAATTTGGGCTCGGTGCGAATGCGTACTATGTTATATTGGAGCTCTATGCCCAAGGAAATGTTATTCTCGCAGATTCAGATTTTATGGTCATGACCCTTCTTCGCTCTCATAGGGATGACGATAAAGGGGTTGCAATCATGTCACGTCATCGTTATCCAGTTGAAATTTGTCGAGTTTTTGAGCGAACAACTGCTGAAAAGTTACAGGAAGCTCTTACGTTGTCTAAGGAACCTGATAACAATGAACCTGTTAAGGATCTTGAAGGTGGAAATATTGTGCCTGATGCACCGAAAGAGAAAAAGGGTAAAAGTAAAGGTGGGAAGCCTGCCGAGTCAAGTAAGAGTACTGGTGATGCTAAAGCCAAACAGGCCACCTTGAAGAATGTTCTTGGGGATGCGTTGGGATATGGGCCTGCTCTGTCTGAGCATATTATATTGGAAGCTGGCCTCATTCCAAACACAAAAGTTTCTAAAGAGAACAAGTTAGATGATAATGCAATTCAGCTATTGGTTGAAGCTGTTGCGAAGTTTGAGGATTGGCTGCATGATGTCATTTCAGGTGATAAAATTCCTGAAGGATACATTTTAATGCAGAAGAATTCAGGAAAAAGTGGCCCCCCATCTGAACCAGGAAGTTCTGTCCAGATATATGATGAATTCTGCCCCATATTATTGAACCAATTCAAATCGAGGGAGCATGTAGAGTTCGAAACATTTGATGCTTCTTTGGATGAGTTCTATAGCAAAATTGAGAGTCAAAGGGCAGAACAACAGCAAAAGGCAAAAGAGAGCTCCGCCACCCAGAAGCTTAATAAAATACGTGTTGACCAGGAAAATCGTGTTCACATGCTAAGGAAAGAAGTTGATCAGTGTGTTAAAATGGCGGAATTGATAGAATATAACTTAGATGATGTGGATGCTGCTATAGTTGCTGTTCGTGTAGCTTTAGCAAAGGGCACAAGTTGGGAGGATATTGCTCGAATGGTGAAGGAGGAGAAGAAATATGGAAACCCTGTTGCTAGCCTCATTGACAAGCTTCAGCTTGAAAAGAATTGTATGACATTGCTGCTGAGCAACAATCTTGATGAAATGGATGATGATGAGAAGACTCTCCCAGCAGATAAGGTGGAAGTTGATTTAGTACTTTCAGCACATGCCAATGCTCGGAGGTGGTATGAACTGAAGAAAAAACAGGAGAGTAAACAGGAGAAGACTGTAACAGCACATGAAAAGGCATTTAAGGCGGCCGAGAAAAAGACTCGTCTCCAGCTGTCACAGGAAAAAGCTGTTGCTAGCATTTCACATATGCGCAAGGTTCACTGGTTTGAGAAATTTAACTGGTTCATCAGCAGTGAGAACTACTTGATTATCAGTGGACGTGATGCTCAACAAAATGAGATGATAGTCAAGCGCTATATGTCAAAAGGAGATCTGTATGTCCATGCGGAGTTGCATGGGGCTTCTAGCACCGTGATAAAGAACCATAGGCCTGACCAACCAGTGCCTCCACTTACATTAAACCAAGCAGGATGCTTCACAGTTTGCCACAGTGCAGCATGGGATTCAAAGATTGTCACTAGTGCTTGGTGGGTTCATCCTCACCAGGTCAGTAAAACTGCTCCTACAGGGGAGTATCTGACAGTTGGAAGTTTTATGATTCGTGGGAAGAAGAATTTTCTTCCTCCACACCCTCTCATTATGGGCTTTGGGTTGTTATTTCGCTTGGACGAAAGCTCCTTGGGCTCCCATCTGAATGAGAGGAGGGTAAGAGGTGAGGAGGAGGGGACGGATGATGTAGATGAAATTGGACCTCTAGAAGAGGTATCTGATTCTGAGTCAGAGAATAAAACTGCAGAGGAAAAACTTGCAGAAGAATCAAAAACCGCTCCTGATTCATCTATACATATCACTAAACCAGATCTGAAAGACCCATCTGAGAACGGCTTACTTAGAAGCAATGCTGAAACAAAAGACTCACATGAAATTTCCATGAAGGACAGGAAGGCCTTGAGTGATGCTGATATTTCTGTAAATGGTGTTTCATCTGTCACCCCCGAACTTGAGGATCTCATTGATAGAGCTCTTGGGCTTGGATCTACAGCTATGTCTGCTAAAAAATATCAAATTGACACTTCTCCAGTTGGTTTGGTAGCGGAACCTAATGTTGAAGAGAATAAGGCTACAGGAAGAGATAAACCTCATATTTCAAAAGCTGAAAGAAGAAAGCTCAAGAAAGGCCAGACTGGTTGTGCTCATGAAGAACAAGCTGAGGTGCAAAATGAAAAATTAAAACATGATAGTTCAGTCAGTCAGCCTGAAAAGGAAGTTCACGAGAAGAAGCCCAGTGGTGGAAAAACCAGCCAATCGAAACTACACAATGTTTCTTTCAGCCAGACTGAAAAGGAAGTTCATGATAAGAAGCCAAGTAGTGGAAAAGCCAGCCGTGGACAAAAGGGTAAACTCAAGAAGATGAAAGAGAAGTATGCTGATCAGGATGAGGAAGAAAGAAGGATCCGTATGGCTTTACTAGCTTCTGCTGGAAGAGTAAAGAAGAATGGAGAGTCCCAAAATGAAACTTCAGCTCCAGCGGAAGATAAGAAACCAAGTCCTGAAGATGCTCCAAAAATATGTTATAGATGTAAAAAGGTAGGTCATCTGTCCCGGGATTGTCCAGAACATCAAGATGGTACATTACATAGTCATGCAAATGGCGGAGTTGAAGATGACCCTCCTGTGGATTTGGATAAATCTACTTCTGAAGTGGACAAGGTGACAATGGAGGAGGATGATATTAATGAGATAGGTGAAGAAGAGAAGGAAAAATTAAATGATGTTGATTACTTGACTGGCAATCCACTGTCCAGTGATATTCTCTTATATGCGGTACCTGTCTGTGGTCCTTATAGCTCAGTCCAGTCTTACAAATATCATGTGAAGATAGTTCCTGGCTCTGTGAAGAGAGGGAAAGCTGCAAAAACTGCCATGAATCTGTTTAGCCACAGGCCGGAAGCAACGGTTAGAGAGAAAGAGTTGATGAAAGCGTGTACTGATCCTGAACTGGTCGCTGCCATTATCAGCAATGTGAAGATAACATCTGCAGGCCTTTCTCAGTTGAAGCAGAAGCAAAAGAAAGTCAAGAAGAGCAGCAGCAAAGCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006913 GO:0008150 GO:0046907 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3441 DUF814 FbpA zf-CCHC

Protein Analysis

1163

Amino Acids

129.63

Weight (kDa)

6.76

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT_N PF05833 19 - 149 1.2e-13 NFACT N-terminal and middle domains
NFACT_N PF05833 230 - 538 2.3e-08 NFACT N-terminal and middle domains
NFACT-R_1 PF05670 553 - 664 2.2e-40 NFACT protein RNA binding domain
zf-CCHC PF00098 985 - 1001 5.6e-06 Zinc knuckle
NFACT-C PF11923 1047 - 1139 3.7e-26 NFACT protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013357)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49930
fragaria_vesca FvH4_4g20770 FvH4_4g20770 FvH4_4g20770
malus_domestica MD13G1148000.v1.1 MD16G1147800.v1.1
prunus_persica Prupe.1G111500_v2.0.a1
pyrus_communis pycom13g12740 pycom16g12630
rosa_chinensis RchiOBHm_Chr4g0426211
rosa_laevigata RLG00000007310
rosa_roxburghii Rroxscaffold_5G00368230
rosa_rugosa Rorug04G0209600
rosa_samantha Rh4AG266500 Rh4BG272500 Rh4CG285700 Rh4DG268800
rosa_wichuraiana Rw4G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1637
Acc65I GGTACC 1 cut(s) 3211
AccB1I GGYRCC 1 cut(s) 3211
AccB7I CCANNNNNTGG 1 cut(s) 2756
AccBSI CCGCTC 1 cut(s) 2213
AccIII TCCGGA 1 cut(s) 12
AclWI GGATC 6 cut(s) 591, 2400, 2428, 2833, 2846, 3371
AcoI YGGCCR 1 cut(s) 1572
AcsI RAATTY 7 cut(s) 492, 894, 925, 983, 1658, 1999, 2310
AcuI CTGAAG 6 cut(s) 921, 1349, 1529, 2889, 2958, 3105
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 8 cut(s) 89, 294, 353, 673, 1470, 3016, 3213, 3373
AfeI AGCGCT 1 cut(s) 1739
AfiI CCNNNNNNNGG 6 cut(s) 1491, 2675, 2756, 3061, 3221, 3331
AflIII ACRYGT 2 cut(s) 84, 1147
AhlI ACTAGT 1 cut(s) 1904
AjiI CACGTC 2 cut(s) 473, 1706
AjnI CCWGG 4 cut(s) 955, 1155, 1929, 3271
Alw21I GWGCWC 4 cut(s) 372, 1121, 2410, 2581
Alw26I GTCTC 1 cut(s) 1595
AlwI GGATC 6 cut(s) 591, 2400, 2428, 2833, 2846, 3371
Ama87I CYCGRG 2 cut(s) 284, 2988
Aor13HI TCCGGA 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 1739
ApeKI GCWGC 9 cut(s) 75, 868, 1247, 1393, 1880, 3296, 3392, 3471, 3474
ApoI RAATTY 7 cut(s) 492, 894, 925, 983, 1658, 1999, 2310
AseI ATTAAT 1 cut(s) 3120
Asp700I GAANNNNTTC 1 cut(s) 1007
Asp718I GGTACC 1 cut(s) 3211
AspLEI GCGC 3 cut(s) 75, 1638, 1740
AspS9I GGNCC 5 cut(s) 10, 737, 941, 2135, 3224
AsuC2I CCSGG 2 cut(s) 2989, 2990
AsuHPI GGTGA 9 cut(s) 16, 689, 899, 1324, 1919, 2111, 2368, 3109, 3143
AsuII TTCGAA 1 cut(s) 1035
AsuNHI GCTAGC 2 cut(s) 1346, 1619
AvaI CYCGRG 2 cut(s) 284, 2988
AvaII GGWCC 3 cut(s) 10, 2135, 3224
BaeGI GKGCMC 1 cut(s) 1286
BamHI GGATCC 1 cut(s) 2838
BanI GGYRCC 1 cut(s) 3211
BanII GRGCYC 5 cut(s) 339, 372, 1121, 2072, 2410
BauI CACGAG 1 cut(s) 2657
BbsI GAAGAC 2 cut(s) 1439, 1544
Bbv12I GWGCWC 4 cut(s) 372, 1121, 2410, 2581
BbvCI CCTCAGC 1 cut(s) 2595
BbvI GCAGC 8 cut(s) 62, 855, 1234, 1380, 1892, 3283, 3379, 3483
BccI CCATC 5 cut(s) 244, 955, 2082, 2266, 3005
BceAI ACGGC 2 cut(s) 2284, 2754
BciT130I CCWGG 4 cut(s) 957, 1157, 1931, 3273
BciVI GTATCC 1 cut(s) 899
BclI TGATCA 2 cut(s) 1192, 2815
BcnI CCSGG 2 cut(s) 2989, 2990
BcoDI GTCTC 1 cut(s) 1595
BcuI ACTAGT 1 cut(s) 1904
BfaI CTAG 6 cut(s) 1347, 1620, 1791, 1905, 2141, 2855
BfmI CTRYAG 7 cut(s) 1066, 1251, 1950, 2178, 2424, 2508, 3423
BfoI RGCGCY 1 cut(s) 1741
BfuI GTATCC 1 cut(s) 899
BglI GCCNNNNNGGC 1 cut(s) 695
BglII AGATCT 2 cut(s) 1756, 2245
BlpI GCTNAGC 1 cut(s) 1397
BmcAI AGTACT 2 cut(s) 673, 1470
Bme1390I CCNGG 6 cut(s) 957, 1157, 1931, 2989, 2990, 3273
Bme18I GGWCC 3 cut(s) 10, 2135, 3224
BmeT110I CYCGRG 2 cut(s) 284, 2988
BmgBI CACGTC 2 cut(s) 473, 1706
BmgT120I GGNCC 5 cut(s) 10, 737, 941, 2135, 3224
BmiI GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2840, 3213
BmrFI CCNGG 6 cut(s) 957, 1157, 1931, 2989, 2990, 3273
BmrI ACTGGG 1 cut(s) 2664
BmsI GCATC 9 cut(s) 601, 670, 712, 1036, 1234, 1699, 1847, 2332, 2932
BmtI GCTAGC 2 cut(s) 1350, 1623
BmuI ACTGGG 1 cut(s) 2664
BpiI GAAGAC 2 cut(s) 1439, 1544
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpmI CTGGAG 3 cut(s) 1577, 2448, 2895
Bpu10I CCTNAGC 2 cut(s) 1178, 2595
Bpu1102I GCTNAGC 1 cut(s) 1397
Bpu14I TTCGAA 1 cut(s) 1035
BpuEI CTTGAG 4 cut(s) 2356, 2408, 2540, 2774
BpuMI CCSGG 2 cut(s) 2989, 2990
BsaAI YACGTR 1 cut(s) 1148
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 5 cut(s) 39, 379, 2064, 2770, 2988
BsaWI WCCGGW 1 cut(s) 12
BsaXI ACNNNNNCTCC 6 cut(s) 80, 110, 2045, 2075, 2893, 2923
Bsc4I CCNNNNNNNGG 6 cut(s) 1491, 2675, 2756, 3061, 3221, 3331
Bse3DI GCAATG 3 cut(s) 1388, 2290, 3412
BseAI TCCGGA 1 cut(s) 12
BseBI CCWGG 4 cut(s) 957, 1157, 1931, 3273
BseDI CCNNGG 5 cut(s) 39, 379, 2064, 2770, 2988
BseLI CCNNNNNNNGG 6 cut(s) 1491, 2675, 2756, 3061, 3221, 3331
BseMI GCAATG 3 cut(s) 1388, 2290, 3412
BseMII CTCAG 7 cut(s) 741, 1388, 2151, 2253, 2586, 3249, 3449
BseRI GAGGAG 5 cut(s) 1334, 2101, 2117, 2120, 3122
BseSI GKGCMC 1 cut(s) 1286
BseX3I CGGCCG 1 cut(s) 1572
BseXI GCAGC 8 cut(s) 62, 855, 1234, 1380, 1892, 3283, 3379, 3483
BseYI CCCAGC 1 cut(s) 1441
BsgI GTGCAG 1 cut(s) 1899
Bsh1285I CGRYCG 1 cut(s) 1575
BshNI GGYRCC 1 cut(s) 3211
BsiEI CGRYCG 1 cut(s) 1575
BsiHKAI GWGCWC 4 cut(s) 372, 1121, 2410, 2581
BsiHKCI CYCGRG 2 cut(s) 284, 2988
BsiSI CCGG 4 cut(s) 13, 150, 2989, 3332
BslFI GGGAC 3 cut(s) 2125, 2875, 2971
BslI CCNNNNNNNGG 6 cut(s) 1491, 2675, 2756, 3061, 3221, 3331
BsmAI GTCTC 1 cut(s) 1595
BsmBI CGTCTC 1 cut(s) 1595
BsmFI GGGAC 3 cut(s) 2125, 2875, 2971
BsmI GAATGC 1 cut(s) 352
BsoBI CYCGRG 2 cut(s) 284, 2988
Bsp119I TTCGAA 1 cut(s) 1035
Bsp1286I GDGCHC 7 cut(s) 339, 372, 1121, 1286, 2072, 2410, 2581
Bsp13I TCCGGA 1 cut(s) 12
Bsp143I GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
Bsp1720I GCTNAGC 1 cut(s) 1397
BspCNI CTCAG 7 cut(s) 742, 1389, 2152, 2254, 2587, 3248, 3448
BspEI TCCGGA 1 cut(s) 12
BspHI TCATGA 3 cut(s) 417, 2581, 2737
BspLI GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2840, 3213
BspMAI CTGCAG 2 cut(s) 2182, 3427
BspOI GCTAGC 2 cut(s) 1350, 1623
BspPI GGATC 6 cut(s) 591, 2400, 2428, 2833, 2846, 3371
BspQI GCTCTTC 1 cut(s) 3461
BspT104I TTCGAA 1 cut(s) 1035
BspT107I GGYRCC 1 cut(s) 3211
BsrBI CCGCTC 1 cut(s) 2213
BsrDI GCAATG 3 cut(s) 1388, 2290, 3412
BssECI CCNNGG 5 cut(s) 39, 379, 2064, 2770, 2988
BssMI GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
BssNI GRCGYC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 2657
BssT1I CCWWGG 2 cut(s) 379, 2064
Bst2BI CACGAG 1 cut(s) 2657
Bst2UI CCWGG 4 cut(s) 957, 1157, 1931, 3273
Bst4CI ACNGT 7 cut(s) 1543, 1798, 1867, 1877, 1969, 3186, 3343
Bst6I CTCTTC 7 cut(s) 2139, 2490, 2509, 2863, 3132, 3278, 3461
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 2 cut(s) 75, 3305
BstBAI YACGTR 1 cut(s) 1148
BstBI TTCGAA 1 cut(s) 1035
BstDSI CCRYGG 1 cut(s) 2770
BstH2I RGCGCY 1 cut(s) 1741
BstHHI GCGC 3 cut(s) 75, 1638, 1740
BstKTI GATC 9 cut(s) 586, 1195, 1759, 2248, 2395, 2423, 2818, 2841, 3379
BstMAI GTCTC 1 cut(s) 1595
BstMBI GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
BstMCI CGRYCG 1 cut(s) 1575
BstMWI GCNNNNNNNGC 9 cut(s) 32, 75, 695, 1256, 1568, 2067, 2435, 2855, 3305
BstNI CCWGG 4 cut(s) 957, 1157, 1931, 3273
BstNSI RCATGY 4 cut(s) 73, 1028, 1177, 1484
BstSCI CCNGG 6 cut(s) 955, 1155, 1929, 2987, 2988, 3271
BstSFI CTRYAG 7 cut(s) 1066, 1251, 1950, 2178, 2424, 2508, 3423
BstSLI GKGCMC 1 cut(s) 1286
BstV1I GCAGC 8 cut(s) 62, 855, 1234, 1380, 1892, 3283, 3379, 3483
BstV2I GAAGAC 2 cut(s) 1439, 1544
BstX2I RGATCY 6 cut(s) 583, 1756, 2245, 2392, 2420, 2838
BstYI RGATCY 6 cut(s) 583, 1756, 2245, 2392, 2420, 2838
BstZI CGGCCG 1 cut(s) 1572
BsuI GTATCC 1 cut(s) 899
BtgI CCRYGG 1 cut(s) 2770
BtrI CACGTC 2 cut(s) 473, 1706
BtsI GCAGTG 2 cut(s) 139, 1684
CciI TCATGA 3 cut(s) 417, 2581, 2737
CfoI GCGC 3 cut(s) 75, 1638, 1740
Cfr13I GGNCC 5 cut(s) 10, 737, 941, 2135, 3224
Cfr9I CCCGGG 1 cut(s) 2988
CseI GACGC 1 cut(s) 178
CsiI ACCWGGT 1 cut(s) 1929
Csp6I GTAC 8 cut(s) 88, 293, 352, 672, 1469, 3015, 3212, 3372
CviQI GTAC 8 cut(s) 88, 293, 352, 672, 1469, 3015, 3212, 3372
DpnI GATC 9 cut(s) 585, 1194, 1758, 2247, 2394, 2422, 2817, 2840, 3378
DpnII GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
EaeI YGGCCR 1 cut(s) 1572
EagI CGGCCG 1 cut(s) 1572
Eam1104I CTCTTC 7 cut(s) 2139, 2490, 2509, 2863, 3132, 3278, 3461
EarI CTCTTC 7 cut(s) 2139, 2490, 2509, 2863, 3132, 3278, 3461
EciI GGCGGA 3 cut(s) 1111, 1226, 3054
Ecl136II GAGCTC 3 cut(s) 370, 1119, 2408
EclXI CGGCCG 1 cut(s) 1572
Eco130I CCWWGG 2 cut(s) 379, 2064
Eco147I AGGCCT 3 cut(s) 1816, 2333, 3429
Eco24I GRGCYC 5 cut(s) 339, 372, 1121, 2072, 2410
Eco32I GATATC 1 cut(s) 94
Eco47I GGWCC 3 cut(s) 10, 2135, 3224
Eco47III AGCGCT 1 cut(s) 1739
Eco52I CGGCCG 1 cut(s) 1572
Eco53kI GAGCTC 3 cut(s) 370, 1119, 2408
Eco57I CTGAAG 6 cut(s) 921, 1349, 1529, 2889, 2958, 3105
Eco88I CYCGRG 2 cut(s) 284, 2988
EcoICRI GAGCTC 3 cut(s) 370, 1119, 2408
EcoRI GAATTC 2 cut(s) 925, 983
EcoRII CCWGG 4 cut(s) 955, 1155, 1929, 3271
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 2 cut(s) 379, 2064
EcoT38I GRGCYC 5 cut(s) 339, 372, 1121, 2072, 2410
ErhI CCWWGG 2 cut(s) 379, 2064
Esp3I CGTCTC 1 cut(s) 1595
FaqI GGGAC 3 cut(s) 2125, 2875, 2971
FauNDI CATATG 1 cut(s) 1632
FbaI TGATCA 2 cut(s) 1192, 2815
FriOI GRGCYC 5 cut(s) 339, 372, 1121, 2072, 2410
FspBI CTAG 6 cut(s) 1347, 1620, 1791, 1905, 2141, 2855
FspI TGCGCA 1 cut(s) 1637
GlaI GCGC 3 cut(s) 74, 1637, 1739
GsaI CCCAGC 1 cut(s) 1445
GsuI CTGGAG 3 cut(s) 1577, 2448, 2895
HaeII RGCGCY 1 cut(s) 1741
HapII CCGG 4 cut(s) 13, 150, 2989, 3332
HgaI GACGC 1 cut(s) 178
HhaI GCGC 3 cut(s) 75, 1638, 1740
Hin1I GRCGYC 1 cut(s) 189
Hin6I GCGC 3 cut(s) 73, 1636, 1738
HinP1I GCGC 3 cut(s) 73, 1636, 1738
HincII GTYRAC 1 cut(s) 1153
HindII GTYRAC 1 cut(s) 1153
HindIII AAGCTT 3 cut(s) 119, 1133, 1361
HpaII CCGG 4 cut(s) 13, 150, 2989, 3332
HphI GGTGA 9 cut(s) 16, 689, 899, 1324, 1919, 2111, 2368, 3109, 3143
HpyCH4III ACNGT 7 cut(s) 1543, 1798, 1867, 1877, 1969, 3186, 3343
HpyCH4IV ACGT 7 cut(s) 84, 107, 213, 472, 545, 1147, 1705
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 75, 695, 1256, 1568, 2067, 2435, 2855, 3305
HpySE526I ACGT 7 cut(s) 84, 107, 213, 472, 545, 1147, 1705
Hsp92I GRCGYC 1 cut(s) 189
HspAI GCGC 3 cut(s) 73, 1636, 1738
Kpn2I TCCGGA 1 cut(s) 12
KpnI GGTACC 1 cut(s) 3215
Ksp22I TGATCA 2 cut(s) 1192, 2815
Kzo9I GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
LguI GCTCTTC 1 cut(s) 3461
Lsp1109I GCAGC 8 cut(s) 62, 855, 1234, 1380, 1892, 3283, 3379, 3483
LweI GCATC 9 cut(s) 601, 670, 712, 1036, 1234, 1699, 1847, 2332, 2932
MabI ACCWGGT 1 cut(s) 1929
MaeI CTAG 6 cut(s) 1347, 1620, 1791, 1905, 2141, 2855
MaeII ACGT 7 cut(s) 84, 107, 213, 472, 545, 1147, 1705
MaeIII GTNAC 9 cut(s) 139, 218, 468, 528, 1543, 1599, 1900, 2374, 3097
MalI GATC 9 cut(s) 585, 1194, 1758, 2247, 2394, 2422, 2817, 2840, 3378
MbiI CCGCTC 1 cut(s) 2213
MboI GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
MflI RGATCY 6 cut(s) 583, 1756, 2245, 2392, 2420, 2838
MhlI GDGCHC 7 cut(s) 339, 372, 1121, 1286, 2072, 2410, 2581
MlyI GAGTC 8 cut(s) 155, 668, 1090, 1429, 1579, 2171, 2294, 2897
MmeI TCCRAC 2 cut(s) 105, 1951
MroI TCCGGA 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 1007
MslI CAYNNNNRTG 8 cut(s) 68, 441, 914, 1485, 2079, 3009, 3027, 3033
MspA1I CMGCKG 2 cut(s) 1597, 2915
MspI CCGG 4 cut(s) 13, 150, 2989, 3332
MspR9I CCNGG 6 cut(s) 957, 1157, 1931, 2989, 2990, 3273
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 4 cut(s) 957, 1157, 1931, 3273
MwoI GCNNNNNNNGC 9 cut(s) 32, 75, 695, 1256, 1568, 2067, 2435, 2855, 3305
NciI CCSGG 2 cut(s) 2989, 2990
NdeI CATATG 1 cut(s) 1632
NdeII GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
NheI GCTAGC 2 cut(s) 1346, 1619
NlaIV GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2840, 3213
NmeAIII GCCGAG 3 cut(s) 64, 682, 1600
NmuCI GTSAC 7 cut(s) 139, 218, 468, 1599, 1900, 2374, 3097
NsbI TGCGCA 1 cut(s) 1637
NspI RCATGY 4 cut(s) 73, 1028, 1177, 1484
NspV TTCGAA 1 cut(s) 1035
PaeI GCATGC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 3 cut(s) 417, 2581, 2737
PceI AGGCCT 3 cut(s) 1816, 2333, 3429
PciSI GCTCTTC 1 cut(s) 3461
PctI GAATGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 1007
PfeI GAWTC 7 cut(s) 401, 1889, 1984, 2156, 2201, 2219, 3313
PflFI GACNNNGTC 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 2756
PleI GAGTC 8 cut(s) 154, 667, 1089, 1429, 1579, 2170, 2294, 2896
PpsI GAGTC 8 cut(s) 154, 667, 1089, 1429, 1579, 2170, 2294, 2896
Ppu21I YACGTR 1 cut(s) 1148
PshBI ATTAAT 1 cut(s) 3120
Psp124BI GAGCTC 3 cut(s) 372, 1121, 2410
Psp6I CCWGG 4 cut(s) 955, 1155, 1929, 3271
PspFI CCCAGC 1 cut(s) 1441
PspGI CCWGG 4 cut(s) 955, 1155, 1929, 3271
PspN4I GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2840, 3213
PspPI GGNCC 5 cut(s) 10, 737, 941, 2135, 3224
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 2 cut(s) 2182, 3427
PsuI RGATCY 6 cut(s) 583, 1756, 2245, 2392, 2420, 2838
PsyI GACNNNGTC 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 1597
RsaI GTAC 8 cut(s) 89, 294, 353, 673, 1470, 3016, 3213, 3373
RsaNI GTAC 8 cut(s) 88, 293, 352, 672, 1469, 3015, 3212, 3372
RseI CAYNNNNRTG 8 cut(s) 68, 441, 914, 1485, 2079, 3009, 3027, 3033
SacI GAGCTC 3 cut(s) 372, 1121, 2410
SapI GCTCTTC 1 cut(s) 3461
Sau3AI GATC 9 cut(s) 583, 1192, 1756, 2245, 2392, 2420, 2815, 2838, 3376
Sau96I GGNCC 5 cut(s) 10, 737, 941, 2135, 3224
ScaI AGTACT 2 cut(s) 673, 1470
SchI GAGTC 8 cut(s) 155, 668, 1090, 1429, 1579, 2171, 2294, 2897
ScrFI CCNGG 6 cut(s) 957, 1157, 1931, 2989, 2990, 3273
SduI GDGCHC 7 cut(s) 339, 372, 1121, 1286, 2072, 2410, 2581
SexAI ACCWGGT 1 cut(s) 1929
SfaNI GCATC 9 cut(s) 601, 670, 712, 1036, 1234, 1699, 1847, 2332, 2932
SfcI CTRYAG 7 cut(s) 1066, 1251, 1950, 2178, 2424, 2508, 3423
Sfr274I CTCGAG 1 cut(s) 284
SfuI TTCGAA 1 cut(s) 1035
SinI GGWCC 3 cut(s) 10, 2135, 3224
SlaI CTCGAG 1 cut(s) 284
SmaI CCCGGG 1 cut(s) 2990
SmiMI CAYNNNNRTG 8 cut(s) 68, 441, 914, 1485, 2079, 3009, 3027, 3033
SmlI CTYRAG 5 cut(s) 284, 2335, 2387, 2555, 2789
SmoI CTYRAG 5 cut(s) 284, 2335, 2387, 2555, 2789
SpeI ACTAGT 1 cut(s) 1904
SphI GCATGC 1 cut(s) 73
SseBI AGGCCT 3 cut(s) 1816, 2333, 3429
SspI AATATT 1 cut(s) 601
SspMI CTAG 6 cut(s) 1347, 1620, 1791, 1905, 2141, 2855
SstI GAGCTC 3 cut(s) 372, 1121, 2410
StuI AGGCCT 3 cut(s) 1816, 2333, 3429
StyD4I CCNGG 6 cut(s) 955, 1155, 1929, 2987, 2988, 3271
StyI CCWWGG 2 cut(s) 379, 2064
TaaI ACNGT 7 cut(s) 1543, 1798, 1867, 1877, 1969, 3186, 3343
TaiI ACGT 7 cut(s) 87, 110, 216, 475, 548, 1150, 1708
TaqI TCGA 6 cut(s) 285, 499, 1016, 1035, 1306, 2693
TatI WGTACW 3 cut(s) 671, 1468, 3371
TauI GCSGC 4 cut(s) 38, 58, 61, 1574
TfiI GAWTC 7 cut(s) 401, 1889, 1984, 2156, 2201, 2219, 3313
TseFI GTSAC 7 cut(s) 139, 218, 468, 1599, 1900, 2374, 3097
TseI GCWGC 9 cut(s) 75, 868, 1247, 1393, 1880, 3296, 3392, 3471, 3474
Tsp45I GTSAC 7 cut(s) 139, 218, 468, 1599, 1900, 2374, 3097
TspGWI ACGGA 2 cut(s) 2129, 2831
TspMI CCCGGG 1 cut(s) 2988
Tth111I GACNNNGTC 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 2756
VpaK11BI GGWCC 3 cut(s) 10, 2135, 3224
VspI ATTAAT 1 cut(s) 3120
XapI RAATTY 7 cut(s) 492, 894, 925, 983, 1658, 1999, 2310
XbaI TCTAGA 1 cut(s) 2140
XceI RCATGY 4 cut(s) 73, 1028, 1177, 1484
XhoI CTCGAG 1 cut(s) 284
XmaI CCCGGG 1 cut(s) 2988
XmnI GAANNNNTTC 1 cut(s) 1007
XspI CTAG 6 cut(s) 1347, 1620, 1791, 1905, 2141, 2855
ZrmI AGTACT 2 cut(s) 673, 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.