pycom13g12740

Nuclear export mediator factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013357
Physical Location & Seq
Reverse (-)
8925595 .. 8932054
6460 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12740.1

Sequence Viewer

Length: 3492 bp
ATGGTGAAGGTCCGGATGAACACCGCTGATGTCGCCGCCGAGGTCAAGTGCTTGCGGCGGCTCATCGGCATGCGCTGCTCCAACGTCTACGATCTCTCCCCCAAGACGTATATGTTGAAGCTCATGAATAGCAGTGGAGTCACAGAGTCCGGCGAGAGCGAGAAGGTTTTTCTATTGATTGAGAGTGGCGTCAGATTGCATACGACTCAATACGTTCGGGATAAGAGTAATACTCCATCTGGGTTTACCTTAAAATTGAGGAAGCACATACGAACACGAAGGCTCGAGGATGTACGGCAGCTTGGATATGATAGGATTGTTCTCTTTCAATTTGGGCTCGGTGAAAATGCGTACTATGTTATATTGGAGCTTTATGCCCAGGGAAATGTTATTCTCGCAGATTCGGATTTTATGGTCATGACCCTTCTTCGCTCCCATAGGGATGACGATAAGGGGGTTGCAATCATGTCACGTCATCGTTATCCAATTGAAATATGTCGAGTTTTTGAGCGAACAACTGCTGAAAAGTTACAGGAAGCTCTTACCTTGTCCAAGGAACCAGATAACAATGAACCCGTTAAGGATCATGAAGGTGGAAATAATGTGCCTGATGCACCAAAAGAGAAAAAGGGTAAAAGTAAAGGTGGGAAGCCTGCTGAGTCAAGTAAAAGTAGCGGTGATACTAAAGCCAAACAGGCCACCTTGAAGAATGTTCTTGGGGATGCGTTGGGCTATGGACCTGCGCTATCTGAGCATGTTATATTAGATGCTGGCCTCATTCCAAATACAAAAGTTTCTAAAGAGAACAAGTTAGACGATAATGCAATTCAGCTTTTGGTTGAAGCTGTTGCGAAGTTTGAGGATTGGCTGCATGATGTCATTTCAGGTGATAAAATTCCTGAAGGGTACATTTTAATGCAGAAGAATTCAGGGAAAAGTGGTCCCCCATCTGAACCAGGGAGTTCTGTCCAGATATATGATGAATTCTGCCCCATATTATTGAACCAATTCAAATCGAGGGAGCATGTGGAGTTTGAAACATTTGATGCTTCTTTGGATGAGTTCTATAGCAAAATTGAGAGTCAAAGGGCAGAACAACAGCAAAAGGCAAAAGAGAGCTCTGCCACCCAGAAGCTTAATAAAATACGTGTTGATCAGGAAAATCGTGTTCACATGCTAAGGAAAGAAGTTGATCAGTGTGTTAAAATGGCGGAATTGATAGAATATAATTTAGATGATGTGGATGCTGCTATAGTAGCTGTTCGTGTAGCTTTAGCAAAGGGCACAAGTTGGGAGGATATTGCTAGAATGGTGAAGGAGGAGAAGAAATATGGAAACCCTGTTGCTAGCCTCATTGACAAGCTTCAGCTTGAAAAGAATTGCATGACATTGCTGCTGAGCAACAATCTTGATGAAATGGATGATGATGAGAAGACTCTCCCTGCAGATAAGGTGGAAGTTGATTTAGCACTTTCAGCACATGCCAATGCTCGGCGGTGGTATGAACTAAAGAAAAAACAGGAGAGCAAACAGGAGAAGACTGTAACAGCACATGAAAAAGCATTTAAGGCAGCGGAGAAAAAGACTCGTCTCCAGCTGTCACAGGAAAAAGCTGTTGCTAGCATTTCACATATGCGCAAGGTTCACTGGTTTGAGAAATTTAACTGGTTCATCAGCAGTGAGAACTATTTGATTATCAGTGGACGTGATGCTCAACAAAACGAGATGATAGTCAAGCGCTATATGTCAAAAGGAGATCTGTATGTCCATGCGGAGTTGCATGGGGCTTCCAGCACCGTGATAAAGAACCATAGGCCTGACCAACCAGTGCCTCCACTTACATTAAACCAAGCAGGGTGCTTCACAGTTTGCCACAGCGCAGCATGGGATTCAAAGATTGTCACTAGTGCGTGGTGGGTTCATCCTCACCAGGTCAGTAAAACTGCTCCTACTGGGGAGTATCTGACAGTTGGAAGTTTTATGATTCGTGGGAAAAAGAATTTTCTTCCTCCACACCCTCTAATTATGGGCTTTGGGTTGTTATTTCGCTTGGACGAGAGCTCATTGGGCTCCCATCTGAATGAGAGGAGGGTAAGAGGTGAGGAGGAAGGGACGAATGATGTTGATGAAAATGGACCTCTAGAAGAGGTATCTGATTCTGAGTCAGAGAATGAAACCATAGAGGAAAAACTTGCAGAAGAACCAAAACCCGCTCCTGATTCATCTATACATATTGATAAACCAGATCTGAAAGACCCATCTGGGAATGGCTTACTTACAAGCAATGCTGAAGCAAAAGACTCAGCTGAAATTCCCATGAAAGAAAAGAAGACCTTTAGTGATGCTGATATTTCTGTAAATGGTGTTTCATCTGTCACCCCAGAACTTGAGGATCTCATTGATAGAGCTCTTGGGCTTGGATCTACAGCTATGTCTGCTAAAAAATATCAGATTGACACTTCTCCAGTTGATTTGGTAGCGGAACCTAATGTTGAGGAGAATAAGGCTACAGGAAGAGAGAAACCTCATATTTCAAAAGCTGAAAGAAGAAAGCTCAAGAAAGGCCAGACTGGTAGTGCTCATGAAGAACAAGCTGATCTGCAAAACGAAAAATTGAAGCAACATGAGATTTCAGTCAGTCAGCCTGAAAAGGAAGTTCATGAGAAGAAGCCTAGTGGTGGAAAAACCAGCCAATCAAAACAACACAATATTTCTGCCAGACAGACCGAAAATGAAGTTCATGATAAGAAGCCAAGTAGTGGAAAAGCCAGCCGTGGACAAAAGGGTAAACTCAAGAAGATGAAAGAGAAGTATGCTGATCAAGATGAGGAAGAAAGAAGGATCCGTATGGCTTTGCTAGCTTCTGCTGGAAGAGTACAGAAGAGTGGAGAGTCCCAAAATGAAAATTCAGCTCTGGCTGAAGATAAGAAACTGAGTCCTGTCGATGCTCCAAAAATATGTTATAAATGTAAAAAGGTAGGTCATCTGTCCCGGGATTGTCCTGAACATCAAGACGGTACGTTACATAGTCATGCAAATGGTGGAGTTGAAGATGACCCTCCTGTGGGTTTGGATAAATCTACTTCTGAAGTTGACAAGGTGACAATGGAGGAGGATGATATTCATGAGATAGGTGAAGAGGAGAAGGAGAAATTAAACGATGTTGATTACTTGACGGGCAATCCACTGCCCAGTGATATTCTCTTATATGCGGTGCCTGTCTGTGGTCCTTATAGCTCCGTCCAGTCTTATAAATATCATGTGAAGATAGTTCCTGGCTCTGTGAAGAGGGGGAAAGCTGCGAAAACTGCCATGAATTTGTTTAGCCACAGGCCGGAAGCAACGGTTAGAGAGAAAGAGTTGATGAAAGCATGCACTGATCCTGAGTTGGTTGCTGCAATTATCGGCAATGTGAAGATAACATCTGCAGGCCTCACTCAGTTGAAGCAGAAGCAAAAGAAAGTCAAGAAGAGCAGCAGCAAAGCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006913 GO:0008150 GO:0046907 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3441 DUF814 FbpA zf-CCHC

Protein Analysis

1164

Amino Acids

129.71

Weight (kDa)

6.56

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT_N PF05833 18 - 149 5.9e-14 NFACT N-terminal and middle domains
NFACT_N PF05833 230 - 538 7.2e-09 NFACT N-terminal and middle domains
NFACT-R_1 PF05670 553 - 664 2.2e-40 NFACT protein RNA binding domain
zf-CCHC PF00098 986 - 1002 2.6e-06 Zinc knuckle
NFACT-C PF11923 1048 - 1141 1e-26 NFACT protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013357)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49930
fragaria_vesca FvH4_4g20770 FvH4_4g20770 FvH4_4g20770
malus_domestica MD13G1148000.v1.1 MD16G1147800.v1.1
prunus_persica Prupe.1G111500_v2.0.a1
pyrus_communis pycom13g12740 pycom16g12630
rosa_chinensis RchiOBHm_Chr4g0426211
rosa_laevigata RLG00000007310
rosa_roxburghii Rroxscaffold_5G00368230
rosa_rugosa Rorug04G0209600
rosa_samantha Rh4AG266500 Rh4BG272500 Rh4CG285700 Rh4DG268800
rosa_wichuraiana Rw4G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2964, 3252
Acc16I TGCGCA 1 cut(s) 1637
Acc36I ACCTGC 1 cut(s) 748
AccB1I GGYRCC 1 cut(s) 3214
AccB7I CCANNNNNTGG 1 cut(s) 2759
AccBSI CCGCTC 1 cut(s) 2213
AccI GTMKAC 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 12
AclWI GGATC 6 cut(s) 591, 2400, 2428, 2836, 2849, 3374
AcsI RAATTY 8 cut(s) 894, 925, 983, 1658, 1999, 2310, 2905, 3316
AcuI CTGAAG 5 cut(s) 921, 1349, 2310, 2940, 3108
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 5 cut(s) 294, 353, 908, 2877, 3019
AfeI AGCGCT 1 cut(s) 1739
AfiI CCNNNNNNNGG 7 cut(s) 1491, 2678, 2759, 3064, 3065, 3224, 3334
AflIII ACRYGT 1 cut(s) 1147
AhlI ACTAGT 1 cut(s) 1904
AjiI CACGTC 2 cut(s) 473, 1706
AjnI CCWGG 4 cut(s) 378, 955, 1929, 3274
Alw21I GWGCWC 4 cut(s) 1121, 2063, 2410, 2581
Alw26I GTCTC 1 cut(s) 1595
AlwI GGATC 6 cut(s) 591, 2400, 2428, 2836, 2849, 3374
Ama87I CYCGRG 2 cut(s) 284, 2991
Aor13HI TCCGGA 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 1739
AoxI GGCC 6 cut(s) 696, 772, 1814, 2563, 3332, 3430
ApoI RAATTY 8 cut(s) 894, 925, 983, 1658, 1999, 2310, 2905, 3316
Asp700I GAANNNNTTC 1 cut(s) 1007
AspLEI GCGC 5 cut(s) 75, 745, 1638, 1740, 1880
AspS9I GGNCC 5 cut(s) 10, 737, 941, 2135, 3227
AsuC2I CCSGG 2 cut(s) 2992, 2993
AsuNHI GCTAGC 3 cut(s) 1346, 1619, 2857
AvaI CYCGRG 2 cut(s) 284, 2991
AvaII GGWCC 5 cut(s) 10, 737, 941, 2135, 3227
BaeGI GKGCMC 1 cut(s) 1286
BamHI GGATCC 1 cut(s) 2841
BanI GGYRCC 1 cut(s) 3214
BanII GRGCYC 5 cut(s) 339, 1121, 2063, 2072, 2410
BbsI GAAGAC 3 cut(s) 1439, 1544, 2336
Bbv12I GWGCWC 4 cut(s) 1121, 2063, 2410, 2581
BccI CCATC 4 cut(s) 244, 955, 2082, 2266
BceAI ACGGC 2 cut(s) 311, 2757
BciT130I CCWGG 4 cut(s) 380, 957, 1931, 3276
BclI TGATCA 3 cut(s) 1153, 1192, 2818
BcnI CCSGG 2 cut(s) 2992, 2993
BcoDI GTCTC 1 cut(s) 1595
BcuI ACTAGT 1 cut(s) 1904
BfaI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
BfmI CTRYAG 6 cut(s) 1066, 1251, 1443, 2424, 2508, 3426
BfoI RGCGCY 1 cut(s) 1741
BfuAI ACCTGC 1 cut(s) 748
BglI GCCNNNNNGGC 1 cut(s) 695
BglII AGATCT 2 cut(s) 1756, 2245
BlpI GCTNAGC 1 cut(s) 1397
Bme1390I CCNGG 6 cut(s) 380, 957, 1931, 2992, 2993, 3276
Bme18I GGWCC 5 cut(s) 10, 737, 941, 2135, 3227
BmeT110I CYCGRG 2 cut(s) 284, 2991
BmgBI CACGTC 2 cut(s) 473, 1706
BmgT120I GGNCC 5 cut(s) 10, 737, 941, 2135, 3227
BmiI GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2843, 3216
BmrFI CCNGG 6 cut(s) 380, 957, 1931, 2992, 2993, 3276
BmrI ACTGGG 2 cut(s) 1962, 3186
BmsI GCATC 8 cut(s) 601, 712, 757, 1036, 1234, 1699, 2332, 2935
BmtI GCTAGC 3 cut(s) 1350, 1623, 2861
BmuI ACTGGG 2 cut(s) 1962, 3186
BpiI GAAGAC 3 cut(s) 1439, 1544, 2336
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpmI CTGGAG 2 cut(s) 1577, 2448
Bpu10I CCTNAGC 1 cut(s) 1178
Bpu1102I GCTNAGC 1 cut(s) 1397
BpuEI CTTGAG 3 cut(s) 2408, 2540, 2777
BpuMI CCSGG 2 cut(s) 2992, 2993
BsaAI YACGTR 1 cut(s) 1148
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 7 cut(s) 39, 378, 379, 552, 956, 2773, 2991
BsaWI WCCGGW 1 cut(s) 12
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 7 cut(s) 1491, 2678, 2759, 3064, 3065, 3224, 3334
Bse1I ACTGG 8 cut(s) 1652, 1670, 1826, 1957, 2465, 2575, 3192, 3244
Bse3DI GCAATG 3 cut(s) 1388, 2290, 3415
BseAI TCCGGA 1 cut(s) 12
BseBI CCWGG 4 cut(s) 380, 957, 1931, 3276
BseDI CCNNGG 7 cut(s) 39, 378, 379, 552, 956, 2773, 2991
BseGI GGATG 9 cut(s) 21, 295, 448, 727, 1063, 1249, 1426, 1921, 3121
BseLI CCNNNNNNNGG 7 cut(s) 1491, 2678, 2759, 3064, 3065, 3224, 3334
BseMI GCAATG 3 cut(s) 1388, 2290, 3415
BseMII CTCAG 8 cut(s) 648, 741, 1388, 2151, 2316, 2924, 3375, 3452
BseNI ACTGG 8 cut(s) 1652, 1670, 1826, 1957, 2465, 2575, 3192, 3244
BseRI GAGGAG 6 cut(s) 1334, 2101, 2117, 2510, 3125, 3155
BseSI GKGCMC 1 cut(s) 1286
BshFI GGCC 6 cut(s) 698, 774, 1816, 2565, 3334, 3432
BshNI GGYRCC 1 cut(s) 3214
BsiHKAI GWGCWC 4 cut(s) 1121, 2063, 2410, 2581
BsiHKCI CYCGRG 2 cut(s) 284, 2991
BsiSI CCGG 4 cut(s) 13, 150, 2992, 3335
BslFI GGGAC 4 cut(s) 927, 2125, 2878, 2974
BslI CCNNNNNNNGG 7 cut(s) 1491, 2678, 2759, 3064, 3065, 3224, 3334
BsmAI GTCTC 1 cut(s) 1595
BsmBI CGTCTC 1 cut(s) 1595
BsmFI GGGAC 4 cut(s) 927, 2125, 2878, 2974
BsnI GGCC 6 cut(s) 698, 774, 1816, 2565, 3334, 3432
BsoBI CYCGRG 2 cut(s) 284, 2991
Bsp1286I GDGCHC 7 cut(s) 339, 1121, 1286, 2063, 2072, 2410, 2581
Bsp13I TCCGGA 1 cut(s) 12
Bsp1720I GCTNAGC 1 cut(s) 1397
BspANI GGCC 6 cut(s) 698, 774, 1816, 2565, 3334, 3432
BspCNI CTCAG 8 cut(s) 649, 742, 1389, 2152, 2315, 2925, 3376, 3451
BspEI TCCGGA 1 cut(s) 12
BspHI TCATGA 7 cut(s) 123, 417, 586, 2581, 2659, 2740, 3124
BspLI GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2843, 3216
BspMAI CTGCAG 2 cut(s) 1447, 3430
BspMI ACCTGC 1 cut(s) 748
BspOI GCTAGC 3 cut(s) 1350, 1623, 2861
BspPI GGATC 6 cut(s) 591, 2400, 2428, 2836, 2849, 3374
BspQI GCTCTTC 1 cut(s) 3464
BspT107I GGYRCC 1 cut(s) 3214
BsrBI CCGCTC 1 cut(s) 2213
BsrDI GCAATG 3 cut(s) 1388, 2290, 3415
BsrI ACTGG 8 cut(s) 1652, 1670, 1826, 1957, 2465, 2575, 3192, 3244
BssECI CCNNGG 7 cut(s) 39, 378, 379, 552, 956, 2773, 2991
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 4 cut(s) 380, 957, 1931, 3276
Bst4CI ACNGT 6 cut(s) 1543, 1798, 1867, 1969, 3017, 3346
Bst6I CTCTTC 7 cut(s) 2139, 2509, 2866, 2876, 3132, 3281, 3464
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 1148
BstDSI CCRYGG 1 cut(s) 2773
BstF5I GGATG 9 cut(s) 21, 295, 448, 727, 1063, 1249, 1426, 1921, 3121
BstH2I RGCGCY 1 cut(s) 1741
BstHHI GCGC 5 cut(s) 75, 745, 1638, 1740, 1880
BstMAI GTCTC 1 cut(s) 1595
BstNI CCWGG 4 cut(s) 380, 957, 1931, 3276
BstNSI RCATGY 6 cut(s) 73, 758, 1028, 1177, 1484, 3375
BstSCI CCNGG 6 cut(s) 378, 955, 1929, 2990, 2991, 3274
BstSFI CTRYAG 6 cut(s) 1066, 1251, 1443, 2424, 2508, 3426
BstSLI GKGCMC 1 cut(s) 1286
BstV2I GAAGAC 3 cut(s) 1439, 1544, 2336
BstX2I RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
BstYI RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
BsuRI GGCC 6 cut(s) 698, 774, 1816, 2565, 3334, 3432
BtgI CCRYGG 1 cut(s) 2773
BtrI CACGTC 2 cut(s) 473, 1706
BtsCI GGATG 9 cut(s) 21, 295, 448, 727, 1063, 1249, 1426, 1921, 3121
BtsI GCAGTG 3 cut(s) 139, 1684, 3185
BtsIMutI CAGTG 9 cut(s) 139, 1202, 1645, 1684, 1705, 1833, 3185, 3199, 3375
BveI ACCTGC 1 cut(s) 748
CciI TCATGA 7 cut(s) 123, 417, 586, 2581, 2659, 2740, 3124
CfoI GCGC 5 cut(s) 75, 745, 1638, 1740, 1880
Cfr13I GGNCC 5 cut(s) 10, 737, 941, 2135, 3227
Cfr9I CCCGGG 1 cut(s) 2991
CseI GACGC 1 cut(s) 178
CsiI ACCWGGT 1 cut(s) 1929
Csp6I GTAC 5 cut(s) 293, 352, 907, 2876, 3018
CviQI GTAC 5 cut(s) 293, 352, 907, 2876, 3018
Eam1104I CTCTTC 7 cut(s) 2139, 2509, 2866, 2876, 3132, 3281, 3464
EarI CTCTTC 7 cut(s) 2139, 2509, 2866, 2876, 3132, 3281, 3464
EciI GGCGGA 1 cut(s) 1226
Ecl136II GAGCTC 3 cut(s) 1119, 2061, 2408
Eco130I CCWWGG 1 cut(s) 552
Eco147I AGGCCT 2 cut(s) 1816, 3432
Eco24I GRGCYC 5 cut(s) 339, 1121, 2063, 2072, 2410
Eco47I GGWCC 5 cut(s) 10, 737, 941, 2135, 3227
Eco47III AGCGCT 1 cut(s) 1739
Eco53kI GAGCTC 3 cut(s) 1119, 2061, 2408
Eco57I CTGAAG 5 cut(s) 921, 1349, 2310, 2940, 3108
Eco88I CYCGRG 2 cut(s) 284, 2991
EcoICRI GAGCTC 3 cut(s) 1119, 2061, 2408
EcoRI GAATTC 2 cut(s) 925, 983
EcoRII CCWGG 4 cut(s) 378, 955, 1929, 3274
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 5 cut(s) 339, 1121, 2063, 2072, 2410
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 1595
FalI AAGNNNNNCTT 2 cut(s) 2318, 2350
FaqI GGGAC 4 cut(s) 927, 2125, 2878, 2974
FauI CCCGC 1 cut(s) 2218
FauNDI CATATG 1 cut(s) 1632
FbaI TGATCA 3 cut(s) 1153, 1192, 2818
FblI GTMKAC 1 cut(s) 87
FokI GGATG 9 cut(s) 28, 302, 455, 734, 1070, 1256, 1433, 1908, 3128
FriOI GRGCYC 5 cut(s) 339, 1121, 2063, 2072, 2410
FspBI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
FspI TGCGCA 1 cut(s) 1637
GlaI GCGC 5 cut(s) 74, 744, 1637, 1739, 1879
GsuI CTGGAG 2 cut(s) 1577, 2448
HaeII RGCGCY 1 cut(s) 1741
HaeIII GGCC 6 cut(s) 698, 774, 1816, 2565, 3334, 3432
HapII CCGG 4 cut(s) 13, 150, 2992, 3335
HgaI GACGC 1 cut(s) 178
HhaI GCGC 5 cut(s) 75, 745, 1638, 1740, 1880
Hin1I GRCGYC 1 cut(s) 189
Hin6I GCGC 5 cut(s) 73, 743, 1636, 1738, 1878
HinP1I GCGC 5 cut(s) 73, 743, 1636, 1738, 1878
HincII GTYRAC 1 cut(s) 3094
HindII GTYRAC 1 cut(s) 3094
HindIII AAGCTT 2 cut(s) 1133, 1361
HpaII CCGG 4 cut(s) 13, 150, 2992, 3335
Hpy166II GTNNAC 8 cut(s) 88, 246, 1171, 1645, 1703, 2777, 2789, 3094
Hpy8I GTNNAC 8 cut(s) 88, 246, 1171, 1645, 1703, 2777, 2789, 3094
HpyAV CCTTC 9 cut(s) 157, 273, 434, 584, 896, 1309, 2102, 2832, 3139
HpyCH4III ACNGT 6 cut(s) 1543, 1798, 1867, 1969, 3017, 3346
HpyCH4IV ACGT 7 cut(s) 84, 107, 213, 472, 1147, 1705, 3020
HpySE526I ACGT 7 cut(s) 84, 107, 213, 472, 1147, 1705, 3020
Hsp92I GRCGYC 1 cut(s) 189
HspAI GCGC 5 cut(s) 73, 743, 1636, 1738, 1878
Kpn2I TCCGGA 1 cut(s) 12
Ksp22I TGATCA 3 cut(s) 1153, 1192, 2818
LguI GCTCTTC 1 cut(s) 3464
LmnI GCTCC 9 cut(s) 83, 367, 437, 1021, 1951, 2075, 2218, 2953, 3242
LweI GCATC 8 cut(s) 601, 712, 757, 1036, 1234, 1699, 2332, 2935
MabI ACCWGGT 1 cut(s) 1929
MaeI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
MaeII ACGT 7 cut(s) 84, 107, 213, 472, 1147, 1705, 3020
MaeIII GTNAC 9 cut(s) 139, 468, 528, 1543, 1599, 1900, 2374, 3021, 3100
MbiI CCGCTC 1 cut(s) 2213
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
MhlI GDGCHC 7 cut(s) 339, 1121, 1286, 2063, 2072, 2410, 2581
MmeI TCCRAC 2 cut(s) 105, 1951
MroI TCCGGA 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 1007
MseI TTAA 9 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 1844, 3155
MslI CAYNNNNRTG 8 cut(s) 68, 441, 591, 914, 1485, 2079, 3030, 3036
MspA1I CMGCKG 4 cut(s) 26, 1574, 1597, 2306
MspI CCGG 4 cut(s) 13, 150, 2992, 3335
MspR9I CCNGG 6 cut(s) 380, 957, 1931, 2992, 2993, 3276
MunI CAATTG 1 cut(s) 486
MvaI CCWGG 4 cut(s) 380, 957, 1931, 3276
NciI CCSGG 2 cut(s) 2992, 2993
NdeI CATATG 1 cut(s) 1632
NheI GCTAGC 3 cut(s) 1346, 1619, 2857
NlaIV GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2843, 3216
NmeAIII GCCGAG 2 cut(s) 64, 1471
NmuCI GTSAC 6 cut(s) 139, 468, 1599, 1900, 2374, 3100
NsbI TGCGCA 1 cut(s) 1637
NspI RCATGY 6 cut(s) 73, 758, 1028, 1177, 1484, 3375
PaeI GCATGC 2 cut(s) 73, 3375
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 7 cut(s) 123, 417, 586, 2581, 2659, 2740, 3124
PasI CCCWGGG 1 cut(s) 379
PceI AGGCCT 2 cut(s) 1816, 3432
PciSI GCTCTTC 1 cut(s) 3464
PdmI GAANNNNTTC 1 cut(s) 1007
PfeI GAWTC 5 cut(s) 401, 1889, 1984, 2156, 2219
PflMI CCANNNNNTGG 1 cut(s) 2759
Ppu21I YACGTR 1 cut(s) 1148
PsiI TTATAA 2 cut(s) 2964, 3252
Psp124BI GAGCTC 3 cut(s) 1121, 2063, 2410
Psp6I CCWGG 4 cut(s) 378, 955, 1929, 3274
PspGI CCWGG 4 cut(s) 378, 955, 1929, 3274
PspN4I GGNNCC 6 cut(s) 558, 943, 2071, 2484, 2843, 3216
PspPI GGNCC 5 cut(s) 10, 737, 941, 2135, 3227
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 2 cut(s) 1447, 3430
PsuI RGATCY 5 cut(s) 1756, 2245, 2392, 2420, 2841
PvuII CAGCTG 2 cut(s) 1597, 2306
RsaI GTAC 5 cut(s) 294, 353, 908, 2877, 3019
RsaNI GTAC 5 cut(s) 293, 352, 907, 2876, 3018
RseI CAYNNNNRTG 8 cut(s) 68, 441, 591, 914, 1485, 2079, 3030, 3036
SacI GAGCTC 3 cut(s) 1121, 2063, 2410
SapI GCTCTTC 1 cut(s) 3464
SaqAI TTAA 9 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 1844, 3155
Sau96I GGNCC 5 cut(s) 10, 737, 941, 2135, 3227
ScrFI CCNGG 6 cut(s) 380, 957, 1931, 2992, 2993, 3276
SduI GDGCHC 7 cut(s) 339, 1121, 1286, 2063, 2072, 2410, 2581
SexAI ACCWGGT 1 cut(s) 1929
SfaNI GCATC 8 cut(s) 601, 712, 757, 1036, 1234, 1699, 2332, 2935
SfcI CTRYAG 6 cut(s) 1066, 1251, 1443, 2424, 2508, 3426
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 5 cut(s) 10, 737, 941, 2135, 3227
SlaI CTCGAG 1 cut(s) 284
SmaI CCCGGG 1 cut(s) 2993
SmiMI CAYNNNNRTG 8 cut(s) 68, 441, 591, 914, 1485, 2079, 3030, 3036
SmlI CTYRAG 4 cut(s) 284, 2387, 2555, 2792
SmoI CTYRAG 4 cut(s) 284, 2387, 2555, 2792
SpeI ACTAGT 1 cut(s) 1904
SphI GCATGC 2 cut(s) 73, 3375
SseBI AGGCCT 2 cut(s) 1816, 3432
SspI AATATT 1 cut(s) 2710
SspMI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
SstI GAGCTC 3 cut(s) 1121, 2063, 2410
StuI AGGCCT 2 cut(s) 1816, 3432
StyD4I CCNGG 6 cut(s) 378, 955, 1929, 2990, 2991, 3274
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 6 cut(s) 1543, 1798, 1867, 1969, 3017, 3346
TaiI ACGT 7 cut(s) 87, 110, 216, 475, 1150, 1708, 3023
TaqI TCGA 4 cut(s) 285, 499, 1016, 2943
TaqII GACCGA 1 cut(s) 2741
TatI WGTACW 1 cut(s) 2875
TauI GCSGC 3 cut(s) 38, 58, 61
TfiI GAWTC 5 cut(s) 401, 1889, 1984, 2156, 2219
Tru1I TTAA 9 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 1844, 3155
Tru9I TTAA 9 cut(s) 251, 579, 914, 1137, 1203, 1566, 1662, 1844, 3155
TscAI CASTG 9 cut(s) 139, 1202, 1652, 1684, 1705, 1833, 3192, 3199, 3382
TseFI GTSAC 6 cut(s) 139, 468, 1599, 1900, 2374, 3100
Tsp45I GTSAC 6 cut(s) 139, 468, 1599, 1900, 2374, 3100
TspGWI ACGGA 2 cut(s) 2834, 3229
TspMI CCCGGG 1 cut(s) 2991
TspRI CASTG 9 cut(s) 139, 1202, 1652, 1684, 1705, 1833, 3192, 3199, 3382
Van91I CCANNNNNTGG 1 cut(s) 2759
VpaK11BI GGWCC 5 cut(s) 10, 737, 941, 2135, 3227
XapI RAATTY 8 cut(s) 894, 925, 983, 1658, 1999, 2310, 2905, 3316
XbaI TCTAGA 1 cut(s) 2140
XceI RCATGY 6 cut(s) 73, 758, 1028, 1177, 1484, 3375
XhoI CTCGAG 1 cut(s) 284
XmaI CCCGGG 1 cut(s) 2991
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 1007
XspI CTAG 7 cut(s) 1305, 1347, 1620, 1905, 2141, 2673, 2858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.