Rroxscaffold_5G00368230

Nuclear export mediator factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013357
Physical Location & Seq
Forward (+)
50053963 .. 50060570
6608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00368230.1

Sequence Viewer

Length: 3405 bp
ATGGTGAAGGTGCGGATGAACACGGCCGACGTGGCCGCCGAGGTCAAGTGCTTGCGGCGGCTTATCGGCATGCGCTGCGCCAATGTCTACGATCTCTCTCCCAAGACCTATATGTTGAAGCTTATGAACAGTAGCGGCGTCACCGAGTCCGGTGAGAGCGAGAAGGTCTTCTTGCTTATCGAAAGCGGCGTCAGATTGCACACTACTGCCTATGTTCGGGACAAGAGTAATACTCCGTCTGGTTTTACGCTCAAAATAAGGAAGCATATACGAACGAGAAGGCTCGAGGATGTGAGGCAGCTGGGATATGATCGGATTATACTATTTCAGTTTGGATTGGGTGCTAATGCCTACTATGTTATATTGGAGCTATATGCGCAAGGAAATATTATTCTTGCAGATTCCGAATATATGGTCATGACTCTTCTTCGCTCGCATAGGGATGATGACAAAGGGGTTGCAATTATGTCACGCCATCGTTATCCAATAGAGATTTGTCGAGTTTTTGAGCGAACAACCTCTGCAAAGTTGCAGGAAGCCCTTACATATTCCAAGGAACCTGATAACAATGAATCTGTAAAAGATTCTGAAGGTCGAAATGATGCATCTGATGCGCCGAAAGAAAAAAAGGGTGGTCGTAAAGGTGGGAAGCCCGTTGAGTCCAGTAAAAAGCCTGGTGATGCTAAAGCCAAACAAGCCACTTTGAAGAATGTTCTAGGGGATGGACTGGGCTATGGACCTGCCCTTTCTGAGCATATTATTTTAGATGCTGGTCTTGTTCCAAATACTAAAGTTGGTAAAGATGAAAAGCTAGATGACAATACACTAAAGCTTTTGCTCGAAGCTGTTACAAAATTTGAGGACTGGCTGCATGATGTTATATCCGGTGAAAAAGTTCCTGAAGGATACATTTTGATGCAGAACAAAACTTCAGGGAAGAATGGTCCCCCATCTGAACCAGGAAGTTCTGGCCAGATATATGATGAATTCTGCCCCATATTATTGAACCAATTCAAGTCGAGGGAGTATGTGCAGTTTGAAACATTTGATGCATCTTTGGATGAGTTCTATAGCAAAATTGAGAGTCAAAGGTCAGAGCAGCAGCAAAAAGCAAAAGAGAGCTCTGCCACCCAGAGGCTTAATAAAATCCGCGTGGATCAGGAAAATCGTGTGCATATGTTAAGGGAAGAGGTTGACCAATGTGTTAAAATGGCGGAATTGATAGAATATAATTTAGAAGATGTAGATGCTGCTATATTAGCTGTTCGTGTATCTCTTGCAAAGGGCATGAGTTGGGAGGATCTTGCTCGTATGGTAAAGGAAGAGAAGAAATCTGGAAATCCTATTGCCGGACTCATTGACAAACTTTATCTTGAAAGGAATTGCATGACATTGCTTTTGAGCAACAATCTTGATGAAATGGATGATGATGAGAAGACTCTCCCTGCAGATAAGGTAGAAGTTGATTTAGCGCTTTCTGCACATGCCAATGCTCGGCGGTGGTATGAACTAAAGAAAAGCAAGGAGAGCAAACAGGAGAAGACTGTAACAGCACATGAAAAAGCATTTAAAGCTGCCGAAAGAAAGACTCGTCTGCAGCTATCGCAGGAAAAAGCTGTTGCTACCATTTCACATATGCGCAAGGTTCACTGGTTTGAGAAATTTAACTGGTTCATCAGCAGTGAAAACTATTTGGTTATCAGTGGACGTGATGCTCAACAAAATGAGATGATAGTCAAGCGCTACATGTCAAAAGGAGATCTGTATGTTCATGCAGATTTGCATGGCGCTTCTAGTACTGTAATAAAGAACCATAGGCCTGAACAACCAGTGCCTCCACTTACTTTAAATCAAGCTGGATGCTACACAGTTTGCCAAAGTGCGGCATGGGATTCAAAGATGGTCACTAGTGCTTGGTGGGTTTATCCTCACCAAGTCAGTAAAACTGCTCCTACAGGGGAGTATCTTACAGTTGGAAGTTTCATGATTCGTGGGAAGAAGAACTTCCTTCCTCCACACCCTCTGATTATGGGCTTTGGGCTGTTATTTCGGTTGGATGAGAGCTCCTTGGGTTCACATCTGAACGAGAGGAGGGTAAGAGGTGAGGAGGAAGGGACAAATGATGCTGATGCAAGTGGGCCTCTGAGTGAGGTTTCTGATTCTGAATCAGAGAAGGATTTGAGAGAGGAAAAAATTCTAGGGGAATCACAAAGTGTACCAGATTCATCTAAACATGTACATAAACCAGATGATATATCCTCTCAGAATAGCTTACCTACAACCGTTGCTAAACAAGTGGACTTAAATGAAAGTTCCATGAAAACCAGGAACATCTTGAATGATCTTGATCAAGACAACACAGTTGATGTTGCTGTGAATGGTGTTGCACCTGTCACCCCACAACTTGAGGACCTCATTGATAGAGCTCTTGGGCTTGGATCTGCATCTATGTCTGTTAAAAATTATAAAGTTGAAACCTCTCCAGTTGATTCGGTAGCAGAACAAAATGTTGAAGAGAATAAGGCTACAGTAAAAGAGAAAGCTTATATCTCGAAGGCTGAAAGAAAAAAGCTCAAGAAAGGCCAGAGTGTTCCTGAAGATGTTAAACCGAAAGTAGATAAAGTGAAAGAAAATGTTTCTGTCTGTCAGCCTGAAAAGGAAGTAAATGATAAGAAGCCAGGTGGTGGAAAAACCAGCCGTGGACAGAGGGGTAAACTCAAGAAGATAAAAGAGAAATATGCCGATCAAGATGAGGAAGAAAGAAGGATTCGTATGGCTTTACTAGCCTCTGCTGGAAATGTACAGAAGAAAGGAGAGTCGCAAAATGGAGAGATAGCTCAAGTGGAGGACAAGAAACCTGGTCCTGAAGAGGGTGCAAAAATATGTTATAAATGTAAGAAGGTAGGTCATCTGTCCCGAGATTGCCAAGAACATCAAGTTGATACATCAGATAGTCATGCAAATGGTGGAGTTGATCACCCTAATGCCTTGGATAAAACTACCTCTGAACTAGACAAGGTGACAATTGAGGAGGATGATATTCATGAGATAGGTGAAGAGGAGAAAGAAAAATTAAATGATGTGGATTACTTGACTGGCAATCCACTGCCCAGTGATATTCTCTTGTATGCAGTGCCTGTATGTGGTCCTTATAATGCAGTCCAATCTTACAAATATCGAGTGAAGATAATTCCTGGCTCTGTGAAGAGAGGAAAAGGTGCAAAAACTGCCATGAACTTGTTTAGCCACATGCCGGACGCAACAGCTAGAGAGAAAGAGTTGATGAAAGCGTGTACTGATCCTGAGCTGGTTGCCGCAATTATTGGAAATGTGAAGATTACAGCTGCAGGCCTCTCTCAATTGAAGCAGAAGCAAAAGAAAGTCAAGAAGAACACAGCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006913 GO:0008150 GO:0046907 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3441 DUF814 FbpA zf-CCHC

Protein Analysis

1134

Amino Acids

126.61

Weight (kDa)

7.12

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT_N PF05833 19 - 149 5.5e-13 NFACT N-terminal and middle domains
NFACT_N PF05833 231 - 518 3.9e-08 NFACT N-terminal and middle domains
NFACT-R_1 PF05670 554 - 665 6.5e-41 NFACT protein RNA binding domain
NFACT-C PF11923 1021 - 1114 9.3e-27 NFACT protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013357)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49930
fragaria_vesca FvH4_4g20770 FvH4_4g20770 FvH4_4g20770
malus_domestica MD13G1148000.v1.1 MD16G1147800.v1.1
prunus_persica Prupe.1G111500_v2.0.a1
pyrus_communis pycom13g12740 pycom16g12630
rosa_chinensis RchiOBHm_Chr4g0426211
rosa_laevigata RLG00000007310
rosa_roxburghii Rroxscaffold_5G00368230
rosa_rugosa Rorug04G0209600
rosa_samantha Rh4AG266500 Rh4BG272500 Rh4CG285700 Rh4DG268800
rosa_wichuraiana Rw4G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2466, 2889, 3153
Acc16I TGCGCA 2 cut(s) 378, 1640
Acc36I ACCTGC 1 cut(s) 748
AccB7I CCANNNNNTGG 1 cut(s) 2684
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 1152
AclWI GGATC 4 cut(s) 1164, 1308, 2446, 3293
AcoI YGGCCR 3 cut(s) 24, 33, 970
AcsI RAATTY 4 cut(s) 854, 986, 1661, 2193
AcuI CTGAAG 5 cut(s) 609, 915, 921, 2616, 2886
AcyI GRCGYC 2 cut(s) 138, 189
AdeI CACNNNGTG 1 cut(s) 2213
AfaI GTAC 5 cut(s) 1798, 2217, 2238, 2802, 3295
AfeI AGCGCT 2 cut(s) 1473, 1742
AfiI CCNNNNNNNGG 9 cut(s) 216, 1134, 1349, 1494, 1882, 2684, 2870, 3143, 3253
AflIII ACRYGT 2 cut(s) 1746, 2233
AhlI ACTAGT 1 cut(s) 1907
AjiI CACGTC 2 cut(s) 31, 1709
AjnI CCWGG 6 cut(s) 673, 958, 2324, 2677, 2857, 3193
Alw21I GWGCWC 3 cut(s) 1124, 2066, 2428
AlwI GGATC 4 cut(s) 1164, 1308, 2446, 3293
AlwNI CAGNNNCTG 1 cut(s) 3137
Ama87I CYCGRG 2 cut(s) 284, 2916
Aor51HI AGCGCT 2 cut(s) 1473, 1742
AoxI GGCC 7 cut(s) 24, 33, 970, 1817, 2138, 2581, 3349
ApeKI GCWGC 9 cut(s) 75, 298, 868, 1099, 1102, 1250, 1574, 1597, 3344
ApoI RAATTY 4 cut(s) 854, 986, 1661, 2193
ArsI GACNNNNNNTTYG 6 cut(s) 174, 206, 399, 431, 1381, 1413
Asp700I GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
AspLEI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
AspS9I GGNCC 6 cut(s) 737, 944, 2138, 2410, 2861, 3146
AvaI CYCGRG 2 cut(s) 284, 2916
AvaII GGWCC 5 cut(s) 737, 944, 2410, 2861, 3146
BalI TGGCCA 1 cut(s) 972
BanII GRGCYC 3 cut(s) 1124, 2066, 2428
BbsI GAAGAC 3 cut(s) 160, 1442, 1547
Bbv12I GWGCWC 3 cut(s) 1124, 2066, 2428
BbvI GCAGC 9 cut(s) 62, 310, 855, 1111, 1114, 1237, 1561, 1609, 3331
BccI CCATC 4 cut(s) 483, 716, 958, 1894
BceAI ACGGC 2 cut(s) 39, 2682
BciT130I CCWGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
BciVI GTATCC 1 cut(s) 899
BclI TGATCA 2 cut(s) 2347, 2974
BcuI ACTAGT 1 cut(s) 1907
BfaI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2783, 3011, 3267
BfmI CTRYAG 6 cut(s) 1069, 1446, 1595, 1953, 2526, 3345
BfoI RGCGCY 3 cut(s) 1475, 1744, 1791
BfuAI ACCTGC 1 cut(s) 748
BfuI GTATCC 1 cut(s) 899
BglI GCCNNNNNGGC 1 cut(s) 32
BglII AGATCT 1 cut(s) 1759
BmcAI AGTACT 1 cut(s) 1798
Bme1390I CCNGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
Bme18I GGWCC 5 cut(s) 737, 944, 2410, 2861, 3146
BmeT110I CYCGRG 2 cut(s) 284, 2916
BmgBI CACGTC 2 cut(s) 31, 1709
BmgT120I GGNCC 6 cut(s) 737, 944, 2138, 2410, 2861, 3146
BmiI GGNNCC 2 cut(s) 558, 946
BmrFI CCNGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
BmrI ACTGGG 2 cut(s) 737, 3105
BmuI ACTGGG 2 cut(s) 737, 3105
BpiI GAAGAC 3 cut(s) 160, 1442, 1547
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpmI CTGGAG 1 cut(s) 2466
Bpu10I CCTNAGC 1 cut(s) 3303
BpuEI CTTGAG 4 cut(s) 2426, 2558, 2702, 2823
BsaBI GATNNNNATC 2 cut(s) 2346, 2443
BsaHI GRCGYC 2 cut(s) 138, 189
BsaJI CCNNGG 5 cut(s) 39, 552, 2067, 2698, 2988
BsaWI WCCGGW 2 cut(s) 149, 884
Bsc4I CCNNNNNNNGG 9 cut(s) 216, 1134, 1349, 1494, 1882, 2684, 2870, 3143, 3253
Bse1I ACTGG 9 cut(s) 663, 732, 869, 1655, 1673, 1829, 2483, 3100, 3111
Bse3DI GCAATG 1 cut(s) 1391
Bse8I GATNNNNATC 2 cut(s) 2346, 2443
BseBI CCWGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
BseDI CCNNGG 5 cut(s) 39, 552, 2067, 2698, 2988
BseGI GGATG 9 cut(s) 21, 295, 448, 727, 1066, 1429, 1865, 2062, 3040
BseJI GATNNNNATC 2 cut(s) 2346, 2443
BseLI CCNNNNNNNGG 9 cut(s) 216, 1134, 1349, 1494, 1882, 2684, 2870, 3143, 3253
BseMI GCAATG 1 cut(s) 1391
BseMII CTCAG 4 cut(s) 741, 2135, 2276, 3294
BseNI ACTGG 9 cut(s) 663, 732, 869, 1655, 1673, 1829, 2483, 3100, 3111
BseRI GAGGAG 4 cut(s) 2104, 2120, 3044, 3074
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 9 cut(s) 62, 310, 855, 1111, 1114, 1237, 1561, 1609, 3331
BseYI CCCAGC 1 cut(s) 301
BsgI GTGCAG 2 cut(s) 1052, 1464
Bsh1236I CGCG 1 cut(s) 1152
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2583, 3351
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 3 cut(s) 1124, 2066, 2428
BsiHKCI CYCGRG 2 cut(s) 284, 2916
BsiSI CCGG 4 cut(s) 150, 885, 1350, 3254
BslFI GGGAC 4 cut(s) 233, 930, 2128, 2899
BslI CCNNNNNNNGG 9 cut(s) 216, 1134, 1349, 1494, 1882, 2684, 2870, 3143, 3253
BsmFI GGGAC 4 cut(s) 233, 930, 2128, 2899
BsnI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2583, 3351
BsoBI CYCGRG 2 cut(s) 284, 2916
Bsp1286I GDGCHC 3 cut(s) 1124, 2066, 2428
Bsp1407I TGTACA 2 cut(s) 2236, 2800
BspANI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2583, 3351
BspCNI CTCAG 4 cut(s) 742, 2136, 2275, 3295
BspFNI CGCG 1 cut(s) 1152
BspHI TCATGA 3 cut(s) 417, 1983, 3043
BspLI GGNNCC 2 cut(s) 558, 946
BspMAI CTGCAG 3 cut(s) 1450, 1599, 3349
BspMI ACCTGC 1 cut(s) 748
BspPI GGATC 4 cut(s) 1164, 1308, 2446, 3293
BsrDI GCAATG 1 cut(s) 1391
BsrGI TGTACA 2 cut(s) 2236, 2800
BsrI ACTGG 9 cut(s) 663, 732, 869, 1655, 1673, 1829, 2483, 3100, 3111
BssECI CCNNGG 5 cut(s) 39, 552, 2067, 2698, 2988
BssNI GRCGYC 2 cut(s) 138, 189
BssT1I CCWWGG 3 cut(s) 552, 2067, 2988
Bst2UI CCWGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
Bst4CI ACNGT 8 cut(s) 131, 1546, 1801, 1870, 1972, 2284, 2362, 2530
Bst6I CTCTTC 7 cut(s) 429, 1182, 1317, 2508, 2862, 3051, 3200
BstACI GRCGYC 2 cut(s) 138, 189
BstAPI GCANNNNNTGC 3 cut(s) 75, 611, 3227
BstAUI TGTACA 2 cut(s) 2236, 2800
BstC8I GCNNGC 4 cut(s) 53, 71, 434, 3349
BstDEI CTNAG 4 cut(s) 750, 2144, 2262, 3303
BstDSI CCRYGG 1 cut(s) 2698
BstF5I GGATG 9 cut(s) 21, 295, 448, 727, 1066, 1429, 1865, 2062, 3040
BstFNI CGCG 1 cut(s) 1152
BstH2I RGCGCY 3 cut(s) 1475, 1744, 1791
BstHHI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
BstNSI RCATGY 5 cut(s) 73, 1487, 1750, 2237, 3253
BstSCI CCNGG 6 cut(s) 673, 958, 2324, 2677, 2857, 3193
BstSFI CTRYAG 6 cut(s) 1069, 1446, 1595, 1953, 2526, 3345
BstUI CGCG 1 cut(s) 1152
BstV1I GCAGC 9 cut(s) 62, 310, 855, 1111, 1114, 1237, 1561, 1609, 3331
BstV2I GAAGAC 3 cut(s) 160, 1442, 1547
BstX2I RGATCY 3 cut(s) 1300, 1759, 2438
BstYI RGATCY 3 cut(s) 1300, 1759, 2438
BstZI CGGCCG 1 cut(s) 24
BsuI GTATCC 1 cut(s) 899
BsuRI GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2583, 3351
BtgI CCRYGG 1 cut(s) 2698
BtrI CACGTC 2 cut(s) 31, 1709
BtsCI GGATG 9 cut(s) 21, 295, 448, 727, 1066, 1429, 1865, 2062, 3040
BtsI GCAGTG 3 cut(s) 1687, 3104, 3138
BtsIMutI CAGTG 7 cut(s) 1648, 1687, 1708, 1836, 3104, 3118, 3138
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 4 cut(s) 53, 71, 434, 3349
CaiI CAGNNNCTG 1 cut(s) 3137
CciI TCATGA 3 cut(s) 417, 1983, 3043
CfoI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
Cfr13I GGNCC 6 cut(s) 737, 944, 2138, 2410, 2861, 3146
CseI GACGC 3 cut(s) 127, 178, 3266
CsiI ACCWGGT 1 cut(s) 2857
Csp6I GTAC 5 cut(s) 1797, 2216, 2237, 2801, 3294
CviQI GTAC 5 cut(s) 1797, 2216, 2237, 2801, 3294
DdeI CTNAG 4 cut(s) 750, 2144, 2262, 3303
DraI TTTAAA 2 cut(s) 1570, 1848
DraIII CACNNNGTG 1 cut(s) 2213
EaeI YGGCCR 3 cut(s) 24, 33, 970
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 7 cut(s) 429, 1182, 1317, 2508, 2862, 3051, 3200
EarI CTCTTC 7 cut(s) 429, 1182, 1317, 2508, 2862, 3051, 3200
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 3 cut(s) 1122, 2064, 2426
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 3 cut(s) 552, 2067, 2988
Eco147I AGGCCT 2 cut(s) 1819, 3351
Eco24I GRGCYC 3 cut(s) 1124, 2066, 2428
Eco47I GGWCC 5 cut(s) 737, 944, 2410, 2861, 3146
Eco47III AGCGCT 2 cut(s) 1473, 1742
Eco52I CGGCCG 1 cut(s) 24
Eco53kI GAGCTC 3 cut(s) 1122, 2064, 2426
Eco57I CTGAAG 5 cut(s) 609, 915, 921, 2616, 2886
Eco88I CYCGRG 2 cut(s) 284, 2916
EcoICRI GAGCTC 3 cut(s) 1122, 2064, 2426
EcoO109I RGGNCCY 1 cut(s) 2410
EcoRI GAATTC 1 cut(s) 986
EcoRII CCWGG 6 cut(s) 673, 958, 2324, 2677, 2857, 3193
EcoT14I CCWWGG 3 cut(s) 552, 2067, 2988
EcoT22I ATGCAT 2 cut(s) 607, 1054
EcoT38I GRGCYC 3 cut(s) 1124, 2066, 2428
ErhI CCWWGG 3 cut(s) 552, 2067, 2988
FalI AAGNNNNNCTT 4 cut(s) 155, 187, 1988, 2020
FaqI GGGAC 4 cut(s) 233, 930, 2128, 2899
FauNDI CATATG 2 cut(s) 1176, 1635
FbaI TGATCA 2 cut(s) 2347, 2974
FblI GTMKAC 1 cut(s) 87
FokI GGATG 9 cut(s) 28, 302, 455, 734, 1073, 1436, 1872, 2069, 3047
FriOI GRGCYC 3 cut(s) 1124, 2066, 2428
FspBI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2783, 3011, 3267
FspI TGCGCA 2 cut(s) 378, 1640
GlaI GCGC 8 cut(s) 74, 79, 378, 615, 1473, 1640, 1742, 1789
GsaI CCCAGC 1 cut(s) 305
GsuI CTGGAG 1 cut(s) 2466
HaeII RGCGCY 3 cut(s) 1475, 1744, 1791
HaeIII GGCC 7 cut(s) 26, 35, 972, 1819, 2140, 2583, 3351
HapII CCGG 4 cut(s) 150, 885, 1350, 3254
HgaI GACGC 3 cut(s) 127, 178, 3266
HhaI GCGC 8 cut(s) 75, 80, 379, 616, 1474, 1641, 1743, 1790
Hin1I GRCGYC 2 cut(s) 138, 189
Hin6I GCGC 8 cut(s) 73, 78, 377, 614, 1472, 1639, 1741, 1788
HinP1I GCGC 8 cut(s) 73, 78, 377, 614, 1472, 1639, 1741, 1788
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 3 cut(s) 119, 830, 2541
HpaII CCGG 4 cut(s) 150, 885, 1350, 3254
Hpy99I CGWCG 1 cut(s) 32
HpyCH4III ACNGT 8 cut(s) 131, 1546, 1801, 1870, 1972, 2284, 2362, 2530
HpyCH4IV ACGT 2 cut(s) 30, 1708
HpyF3I CTNAG 4 cut(s) 750, 2144, 2262, 3303
HpySE526I ACGT 2 cut(s) 30, 1708
Hsp92I GRCGYC 2 cut(s) 138, 189
HspAI GCGC 8 cut(s) 73, 78, 377, 614, 1472, 1639, 1741, 1788
Ksp22I TGATCA 2 cut(s) 2347, 2974
LmnI GCTCC 3 cut(s) 367, 1954, 2069
Lsp1109I GCAGC 9 cut(s) 62, 310, 855, 1111, 1114, 1237, 1561, 1609, 3331
MabI ACCWGGT 1 cut(s) 2857
MaeI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2783, 3011, 3267
MaeII ACGT 2 cut(s) 30, 1708
MaeIII GTNAC 7 cut(s) 139, 468, 847, 1546, 1903, 2392, 3019
MfeI CAATTG 2 cut(s) 3024, 3359
MflI RGATCY 3 cut(s) 1300, 1759, 2438
MhlI GDGCHC 3 cut(s) 1124, 2066, 2428
MlsI TGGCCA 1 cut(s) 972
MluNI TGGCCA 1 cut(s) 972
MlyI GAGTC 8 cut(s) 155, 415, 668, 1093, 1347, 1432, 1582, 2825
MmeI TCCRAC 2 cut(s) 1954, 2034
Mox20I TGGCCA 1 cut(s) 972
Mph1103I ATGCAT 2 cut(s) 607, 1054
MroXI GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
MscI TGGCCA 1 cut(s) 972
MslI CAYNNNNRTG 5 cut(s) 441, 914, 1488, 2961, 2982
Msp20I TGGCCA 1 cut(s) 972
MspA1I CMGCKG 2 cut(s) 301, 3344
MspI CCGG 4 cut(s) 150, 885, 1350, 3254
MspR9I CCNGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
MteI GCGCNGCGC 1 cut(s) 76
MunI CAATTG 2 cut(s) 3024, 3359
MvaI CCWGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
MvnI CGCG 1 cut(s) 1152
NdeI CATATG 2 cut(s) 1176, 1635
NlaIV GGNNCC 2 cut(s) 558, 946
NmeAIII GCCGAG 2 cut(s) 64, 1474
NmuCI GTSAC 5 cut(s) 139, 468, 1903, 2392, 3019
NsbI TGCGCA 2 cut(s) 378, 1640
NsiI ATGCAT 2 cut(s) 607, 1054
NspI RCATGY 5 cut(s) 73, 1487, 1750, 2237, 3253
PaeI GCATGC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 3 cut(s) 417, 1983, 3043
PceI AGGCCT 2 cut(s) 1819, 3351
PciI ACATGT 2 cut(s) 1746, 2233
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 186
PdmI GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
PflMI CCANNNNNTGG 1 cut(s) 2684
PleI GAGTC 8 cut(s) 154, 415, 667, 1092, 1347, 1432, 1582, 2824
PpsI GAGTC 8 cut(s) 154, 415, 667, 1092, 1347, 1432, 1582, 2824
PpuMI RGGWCCY 1 cut(s) 2410
PscI ACATGT 2 cut(s) 1746, 2233
PsiI TTATAA 3 cut(s) 2466, 2889, 3153
Psp124BI GAGCTC 3 cut(s) 1124, 2066, 2428
Psp5II RGGWCCY 1 cut(s) 2410
Psp6I CCWGG 6 cut(s) 673, 958, 2324, 2677, 2857, 3193
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 6 cut(s) 673, 958, 2324, 2677, 2857, 3193
PspN4I GGNNCC 2 cut(s) 558, 946
PspPI GGNCC 6 cut(s) 737, 944, 2138, 2410, 2861, 3146
PspPPI RGGWCCY 1 cut(s) 2410
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 3 cut(s) 1450, 1599, 3349
PstNI CAGNNNCTG 1 cut(s) 3137
PsuI RGATCY 3 cut(s) 1300, 1759, 2438
PvuII CAGCTG 2 cut(s) 301, 3344
RsaI GTAC 5 cut(s) 1798, 2217, 2238, 2802, 3295
RsaNI GTAC 5 cut(s) 1797, 2216, 2237, 2801, 3294
RseI CAYNNNNRTG 5 cut(s) 441, 914, 1488, 2961, 2982
SacI GAGCTC 3 cut(s) 1124, 2066, 2428
Sau96I GGNCC 6 cut(s) 737, 944, 2138, 2410, 2861, 3146
ScaI AGTACT 1 cut(s) 1798
SchI GAGTC 8 cut(s) 155, 415, 668, 1093, 1347, 1432, 1582, 2825
ScrFI CCNGG 6 cut(s) 675, 960, 2326, 2679, 2859, 3195
SduI GDGCHC 3 cut(s) 1124, 2066, 2428
SexAI ACCWGGT 1 cut(s) 2857
SfcI CTRYAG 6 cut(s) 1069, 1446, 1595, 1953, 2526, 3345
SfiI GGCCNNNNNGGCC 1 cut(s) 32
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 5 cut(s) 737, 944, 2410, 2861, 3146
SlaI CTCGAG 1 cut(s) 284
SmiMI CAYNNNNRTG 5 cut(s) 441, 914, 1488, 2961, 2982
SmlI CTYRAG 5 cut(s) 284, 2405, 2573, 2717, 2838
SmoI CTYRAG 5 cut(s) 284, 2405, 2573, 2717, 2838
SpeI ACTAGT 1 cut(s) 1907
SphI GCATGC 1 cut(s) 73
SseBI AGGCCT 2 cut(s) 1819, 3351
SspI AATATT 1 cut(s) 388
SspMI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2783, 3011, 3267
SstI GAGCTC 3 cut(s) 1124, 2066, 2428
StuI AGGCCT 2 cut(s) 1819, 3351
StyD4I CCNGG 6 cut(s) 673, 958, 2324, 2677, 2857, 3193
StyI CCWWGG 3 cut(s) 552, 2067, 2988
TaaI ACNGT 8 cut(s) 131, 1546, 1801, 1870, 1972, 2284, 2362, 2530
TaiI ACGT 2 cut(s) 33, 1711
TaqI TCGA 8 cut(s) 180, 285, 499, 595, 840, 1019, 2552, 3178
TatI WGTACW 4 cut(s) 1796, 2236, 2800, 3293
TauI GCSGC 7 cut(s) 38, 58, 61, 138, 189, 1886, 3317
TscAI CASTG 7 cut(s) 1655, 1687, 1708, 1836, 3111, 3118, 3138
TseFI GTSAC 5 cut(s) 139, 468, 1903, 2392, 3019
TseI GCWGC 9 cut(s) 75, 298, 868, 1099, 1102, 1250, 1574, 1597, 3344
Tsp45I GTSAC 5 cut(s) 139, 468, 1903, 2392, 3019
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 7 cut(s) 1655, 1687, 1708, 1836, 3111, 3118, 3138
Van91I CCANNNNNTGG 1 cut(s) 2684
VpaK11BI GGWCC 5 cut(s) 737, 944, 2410, 2861, 3146
XapI RAATTY 4 cut(s) 854, 986, 1661, 2193
XceI RCATGY 5 cut(s) 73, 1487, 1750, 2237, 3253
XhoI CTCGAG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 5 cut(s) 167, 894, 1010, 2003, 2193
XspI CTAG 8 cut(s) 716, 812, 1794, 1908, 2198, 2783, 3011, 3267
ZrmI AGTACT 1 cut(s) 1798
Zsp2I ATGCAT 2 cut(s) 607, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.