FvH4_4g20910

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015600
Physical Location & Seq
Reverse (-)
24023491 .. 24025868
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20910.t1

Sequence Viewer

Length: 1158 bp
ATGATTGTGAGTCAGAATATGGTGGCTGAGGCCGAGATTATTTGCCAGCAGAGCCTGCTGGACGTGAAGTACAGGCGCGTGCAGATCGACGTGGGGTCGCCGCCGTCTTCTTCTCCGAGCTTCGGAGAGGTCTGCGTCTCCGAGACGGTTTCGGCCGAGGTGAAGCGCTTCGAATCGGTTGTGAGGTGCTCAGAGACTATTGCTAATGCTGTAATGGAGGCCCCTGCAGTAAAGTTTGTTCCAAAGATTCACTCAGGAAGCCATAGTGAGATTGGAGTAAGACATTCCATGGATGACGATCACATACGGATCGACGACCTATCTGCCCATGTTGGGCCTCTCTTTAATAGTTCCTTGCCGAGTGCATTTTATGCAGTTTTTGATGGGCATGGCGGGCCTGAAGCAGCTACTTATATCAAGAGGAATGCCATGAGACTATTTTTTGAAGATGCTGATTTTCCGCACACAGTAGATTTTGATGGTGCTTTCTTCAAAGAGTTGGAGAACTCACATAAGAAAGCATTTTTACTGGCAGACCAGGCCTTGGCTGATGAACACAGTGTGGATGCTTCATGTGGAACCACCGCATTGACTGCCCTAGTACTTGGAAGGCAGTTGCTCGTAGCAAATGCTGGTGACTGTCGGGCAGTTCTTTGTAGGAAAGGAGTTGCATTTGATATGTCCCAAGATCATAAGCCTACTTACTTGCCGGAACGTAGGCGGGTCGAGCAGTTGGGTGGTTATATTGATGATGGGTATCTTAATGGTTATCTTTCAGTCACCCGAACCCTTGGAAATTGGGACTTCAAATTACCACTTGGCTCCTCGTCACCTCTCATTGCTGAGCCAGATGTTCAACAGAGTATGTTAACAGAGGATGATGAGTTTCTGATACTTAGTTGTGATGGCATATGGGATGTCATGTCTAGCCAATATGCGGTGAGTCTTGTAAGGCGTGAGCTGAGGAAGCACAATGATCCAGAACAGTGTGCCAGAGAACTTGTGAAAGAAGCGCTACGCCTTCATGCATCTGACAACCTCACTGTTATTGTTGTATGCCTCTCTTCTCCTGATCGTCCAGTCAAGTCTCGCTCTGAGGGACCAAGATTAAGGAACTGCAGTTTTTCAAAGTTGAGAAGCTTGCTAGAAGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

386

Amino Acids

42.49

Weight (kDa)

5.34

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 85 - 346 1.7e-65 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 544
AccII CGCG 1 cut(s) 78
AciI CCGC 6 cut(s) 101, 393, 461, 585, 721, 938
AclWI GGATC 2 cut(s) 317, 971
AcoI YGGCCR 1 cut(s) 153
AcuI CTGAAG 1 cut(s) 420
AdeI CACNNNGTG 1 cut(s) 562
AfaI GTAC 2 cut(s) 71, 603
AfeI AGCGCT 2 cut(s) 167, 1014
AfiI CCNNNNNNNGG 4 cut(s) 122, 333, 544, 937
AgsI TTSAA 5 cut(s) 446, 493, 808, 857, 1128
AhdI GACNNNNNGTC 1 cut(s) 94
AjiI CACGTC 2 cut(s) 64, 91
AjnI CCWGG 1 cut(s) 537
AluBI AGCT 4 cut(s) 120, 407, 961, 1140
AluI AGCT 4 cut(s) 120, 407, 961, 1140
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 5 cut(s) 137, 142, 188, 427, 1092
AlwI GGATC 2 cut(s) 317, 971
AlwNI CAGNNNCTG 1 cut(s) 55
Aor51HI AGCGCT 2 cut(s) 167, 1014
AoxI GGCC 6 cut(s) 30, 153, 219, 335, 395, 540
ApeKI GCWGC 1 cut(s) 404
Asp700I GAANNNNTTC 1 cut(s) 167
AspLEI GCGC 3 cut(s) 78, 168, 1015
AspS9I GGNCC 4 cut(s) 220, 335, 395, 1100
AsuHPI GGTGA 5 cut(s) 172, 647, 772, 822, 952
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 1 cut(s) 1100
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 191
BbvCI CCTCAGC 2 cut(s) 27, 962
BbvI GCAGC 1 cut(s) 416
BccI CCATC 4 cut(s) 377, 473, 746, 899
BceAI ACGGC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 539
BcoDI GTCTC 5 cut(s) 137, 142, 188, 427, 1092
BfaI CTAG 3 cut(s) 599, 927, 1145
BfmI CTRYAG 2 cut(s) 225, 1117
BfoI RGCGCY 2 cut(s) 169, 1016
BisI GCNGC 2 cut(s) 101, 405
BlpI GCTNAGC 1 cut(s) 843
BlsI GCNGC 2 cut(s) 102, 406
BmcAI AGTACT 1 cut(s) 603
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 1 cut(s) 1100
BmeRI GACNNNNNGTC 1 cut(s) 94
BmgBI CACGTC 2 cut(s) 64, 91
BmgT120I GGNCC 4 cut(s) 220, 335, 395, 1100
BmiI GGNNCC 4 cut(s) 222, 580, 823, 1101
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 3 cut(s) 439, 556, 1037
BpiI GAAGAC 1 cut(s) 99
Bpu10I CCTNAGC 2 cut(s) 27, 962
Bpu1102I GCTNAGC 1 cut(s) 843
Bpu14I TTCGAA 1 cut(s) 171
BsaBI GATNNNNATC 2 cut(s) 297, 756
BsaJI CCNNGG 4 cut(s) 156, 288, 543, 790
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 333, 544, 937
Bse1I ACTGG 2 cut(s) 534, 1079
Bse3DI GCAATG 1 cut(s) 837
Bse8I GATNNNNATC 2 cut(s) 297, 756
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 4 cut(s) 156, 288, 543, 790
BseGI GGATG 4 cut(s) 298, 571, 883, 922
BseJI GATNNNNATC 2 cut(s) 297, 756
BseLI CCNNNNNNNGG 4 cut(s) 122, 333, 544, 937
BseMI GCAATG 1 cut(s) 837
BseMII CTCAG 6 cut(s) 18, 204, 267, 834, 953, 1086
BseNI ACTGG 2 cut(s) 534, 1079
BseRI GAGGAG 1 cut(s) 814
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 6 cut(s) 32, 155, 221, 337, 397, 542
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 191
BsiSI CCGG 1 cut(s) 710
BslFI GGGAC 3 cut(s) 667, 815, 1113
BslI CCNNNNNNNGG 4 cut(s) 122, 333, 544, 937
BsmAI GTCTC 5 cut(s) 137, 142, 188, 427, 1092
BsmBI CGTCTC 2 cut(s) 137, 142
BsmFI GGGAC 3 cut(s) 667, 815, 1113
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 6 cut(s) 32, 155, 221, 337, 397, 542
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
Bsp1720I GCTNAGC 1 cut(s) 843
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 6 cut(s) 101, 393, 461, 585, 721, 938
BspANI GGCC 6 cut(s) 32, 155, 221, 337, 397, 542
BspCNI CTCAG 6 cut(s) 19, 203, 266, 835, 954, 1087
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 4 cut(s) 222, 580, 823, 1101
BspMAI CTGCAG 2 cut(s) 229, 1121
BspPI GGATC 2 cut(s) 317, 971
BspT104I TTCGAA 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 837
BsrI ACTGG 2 cut(s) 534, 1079
BssECI CCNNGG 4 cut(s) 156, 288, 543, 790
BssMI GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
BssT1I CCWWGG 3 cut(s) 288, 543, 790
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 6 cut(s) 148, 469, 560, 641, 987, 1045
Bst6I CTCTTC 1 cut(s) 1069
BstAPI GCANNNNNTGC 3 cut(s) 55, 371, 593
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 5 cut(s) 47, 56, 80, 395, 1142
BstDEI CTNAG 7 cut(s) 27, 190, 253, 843, 896, 962, 1095
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 4 cut(s) 298, 571, 883, 922
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 2 cut(s) 169, 1016
BstHHI GCGC 3 cut(s) 78, 168, 1015
BstKTI GATC 6 cut(s) 87, 301, 312, 691, 979, 1075
BstMAI GTCTC 5 cut(s) 137, 142, 188, 427, 1092
BstMBI GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 8 cut(s) 51, 55, 371, 394, 539, 593, 727, 967
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 2 cut(s) 225, 1117
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 416
BstV2I GAAGAC 1 cut(s) 99
BstZI CGGCCG 1 cut(s) 153
BsuRI GGCC 6 cut(s) 32, 155, 221, 337, 397, 542
BtgI CCRYGG 1 cut(s) 288
BtrI CACGTC 2 cut(s) 64, 91
BtsCI GGATG 4 cut(s) 298, 571, 883, 922
BtsIMutI CAGTG 3 cut(s) 565, 992, 1041
Cac8I GCNNGC 5 cut(s) 47, 56, 80, 395, 1142
CaiI CAGNNNCTG 1 cut(s) 55
CfoI GCGC 3 cut(s) 78, 168, 1015
Cfr13I GGNCC 4 cut(s) 220, 335, 395, 1100
CseI GACGC 1 cut(s) 124
Csp6I GTAC 2 cut(s) 70, 602
CviAII CATG 7 cut(s) 289, 329, 389, 430, 573, 922, 1025
CviQI GTAC 2 cut(s) 70, 602
DdeI CTNAG 7 cut(s) 27, 190, 253, 843, 896, 962, 1095
DpnI GATC 6 cut(s) 86, 300, 311, 690, 978, 1074
DpnII GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
DraIII CACNNNGTG 1 cut(s) 562
DriI GACNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 153
EagI CGGCCG 1 cut(s) 153
Eam1104I CTCTTC 1 cut(s) 1069
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 1069
EclXI CGGCCG 1 cut(s) 153
Eco130I CCWWGG 3 cut(s) 288, 543, 790
Eco147I AGGCCT 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 1100
Eco47III AGCGCT 2 cut(s) 167, 1014
Eco52I CGGCCG 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 537
EcoT14I CCWWGG 3 cut(s) 288, 543, 790
EcoT22I ATGCAT 1 cut(s) 1030
ErhI CCWWGG 3 cut(s) 288, 543, 790
Esp3I CGTCTC 2 cut(s) 137, 142
FaeI CATG 7 cut(s) 292, 332, 392, 433, 576, 925, 1028
FaqI GGGAC 3 cut(s) 667, 815, 1113
FatI CATG 7 cut(s) 288, 328, 388, 429, 572, 921, 1024
FauI CCCGC 2 cut(s) 386, 714
FauNDI CATATG 1 cut(s) 911
Fnu4HI GCNGC 2 cut(s) 101, 405
FokI GGATG 4 cut(s) 305, 578, 890, 929
Fsp4HI GCNGC 2 cut(s) 101, 405
FspBI CTAG 3 cut(s) 599, 927, 1145
GlaI GCGC 3 cut(s) 77, 167, 1014
GluI GCNGC 2 cut(s) 101, 405
HaeII RGCGCY 2 cut(s) 169, 1016
HaeIII GGCC 6 cut(s) 32, 155, 221, 337, 397, 542
HapII CCGG 1 cut(s) 710
HgaI GACGC 1 cut(s) 124
HhaI GCGC 3 cut(s) 78, 168, 1015
Hin1II CATG 7 cut(s) 292, 332, 392, 433, 576, 925, 1028
Hin6I GCGC 3 cut(s) 76, 166, 1013
HinP1I GCGC 3 cut(s) 76, 166, 1013
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HindIII AAGCTT 1 cut(s) 1138
HinfI GANTC 4 cut(s) 10, 173, 247, 943
HpaI GTTAAC 1 cut(s) 870
HpaII CCGG 1 cut(s) 710
HphI GGTGA 5 cut(s) 172, 647, 772, 822, 952
Hpy166II GTNNAC 1 cut(s) 870
Hpy188I TCNGA 8 cut(s) 15, 117, 125, 142, 193, 891, 1033, 1096
Hpy188III TCNNGA 4 cut(s) 255, 418, 980, 1070
Hpy8I GTNNAC 1 cut(s) 870
Hpy99I CGWCG 2 cut(s) 92, 317
HpyAV CCTTC 3 cut(s) 603, 1031, 1142
HpyCH4III ACNGT 6 cut(s) 148, 469, 560, 641, 987, 1045
HpyCH4IV ACGT 3 cut(s) 63, 90, 715
HpyCH4V TGCA 7 cut(s) 82, 227, 365, 374, 671, 1028, 1119
HpyF10VI GCNNNNNNNGC 8 cut(s) 51, 55, 371, 394, 539, 593, 727, 967
HpyF3I CTNAG 7 cut(s) 27, 190, 253, 843, 896, 962, 1095
HpySE526I ACGT 3 cut(s) 63, 90, 715
Hsp92II CATG 7 cut(s) 292, 332, 392, 433, 576, 925, 1028
HspAI GCGC 3 cut(s) 76, 166, 1013
KspAI GTTAAC 1 cut(s) 870
Kzo9I GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 1 cut(s) 416
LweI GCATC 3 cut(s) 439, 556, 1037
MaeI CTAG 3 cut(s) 599, 927, 1145
MaeII ACGT 3 cut(s) 63, 90, 715
MaeIII GTNAC 3 cut(s) 635, 778, 828
MalI GATC 6 cut(s) 86, 300, 311, 690, 978, 1074
MboI GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
MboII GAAGA 5 cut(s) 99, 102, 458, 481, 1056
MfeI CAATTG 1 cut(s) 1153
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 3 cut(s) 796, 809, 1153
MlyI GAGTC 2 cut(s) 19, 952
MmeI TCCRAC 1 cut(s) 480
Mph1103I ATGCAT 1 cut(s) 1030
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 4 cut(s) 345, 762, 869, 1109
MspI CCGG 1 cut(s) 710
MspR9I CCNGG 1 cut(s) 539
MunI CAATTG 1 cut(s) 1153
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 1 cut(s) 539
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 8 cut(s) 51, 55, 371, 394, 539, 593, 727, 967
NcoI CCATGG 1 cut(s) 288
NdeI CATATG 1 cut(s) 911
NdeII GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
NlaIII CATG 7 cut(s) 292, 332, 392, 433, 576, 925, 1028
NlaIV GGNNCC 4 cut(s) 222, 580, 823, 1101
NmeAIII GCCGAG 3 cut(s) 58, 181, 384
NmuCI GTSAC 3 cut(s) 635, 778, 828
NsiI ATGCAT 1 cut(s) 1030
NspV TTCGAA 1 cut(s) 171
PceI AGGCCT 1 cut(s) 542
PctI GAATGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 173, 247
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 2 cut(s) 102, 406
PleI GAGTC 2 cut(s) 18, 951
PpsI GAGTC 2 cut(s) 18, 951
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 4 cut(s) 222, 580, 823, 1101
PspPI GGNCC 4 cut(s) 220, 335, 395, 1100
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PstI CTGCAG 2 cut(s) 229, 1121
PstNI CAGNNNCTG 1 cut(s) 55
RsaI GTAC 2 cut(s) 71, 603
RsaNI GTAC 2 cut(s) 70, 602
SaqAI TTAA 4 cut(s) 345, 762, 869, 1109
SatI GCNGC 2 cut(s) 101, 405
Sau3AI GATC 6 cut(s) 84, 298, 309, 688, 976, 1072
Sau96I GGNCC 4 cut(s) 220, 335, 395, 1100
ScaI AGTACT 1 cut(s) 603
SchI GAGTC 2 cut(s) 19, 952
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 1 cut(s) 191
SfaNI GCATC 3 cut(s) 439, 556, 1037
SfcI CTRYAG 2 cut(s) 225, 1117
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 1 cut(s) 1100
Sse9I AATT 3 cut(s) 796, 809, 1153
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 6 cut(s) 101, 393, 461, 585, 721, 938
SspMI CTAG 3 cut(s) 599, 927, 1145
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 3 cut(s) 288, 543, 790
TaaI ACNGT 6 cut(s) 148, 469, 560, 641, 987, 1045
TaiI ACGT 3 cut(s) 66, 93, 718
TaqI TCGA 4 cut(s) 87, 171, 312, 726
TasI AATT 3 cut(s) 796, 809, 1153
TatI WGTACW 2 cut(s) 69, 601
TauI GCSGC 1 cut(s) 103
TfiI GAWTC 2 cut(s) 173, 247
Tru1I TTAA 4 cut(s) 345, 762, 869, 1109
Tru9I TTAA 4 cut(s) 345, 762, 869, 1109
TscAI CASTG 3 cut(s) 565, 992, 1048
TseFI GTSAC 3 cut(s) 635, 778, 828
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 3 cut(s) 635, 778, 828
TspDTI ATGAA 3 cut(s) 561, 567, 1013
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 3 cut(s) 565, 992, 1048
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 3 cut(s) 599, 927, 1145
ZrmI AGTACT 1 cut(s) 603
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.