MD13G1147300.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015600
Physical Location & Seq
Forward (+)
11528602 .. 11531131
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1147300.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGATTGTGAGCCAGGAAATGGTGGCTGAGGCGGAGGTTATCTGCCAGGAGACGGTGCTGGACATGAACTACGGCGTCTGCGTGGCCAAGGAGCACGAACTACAGATCGACGTGGCGTCCTCCTCGCCTACCTCTCCGAGCTTCGGCGAGGTTGTCGTCGCCGAGACGATTTTCCCTGAGATTTCTCGCTTCGAATCGGTTCTGAGTTGCACAGAGGCGATTAATAATGCTGGAATGGAGTCTTCTGCAGTCAAGTTTGTTCCGAACATTCATTCAGGAAGCCATAGTGACATTGGACCAAGGGATTCCATGGATGACGAGCACATTCGGATTGATGATCTATCTGCCCATGTTGGGGCTCTCTTCAAGTGTCCCTTTCCAAGTGCATTTTATGCGGTGTTTGATGGTCACGGAGGGCCCGAGGCAGCTGCTTATATCAAGAGGAATGCTATGAGACTATTCTTTGAAGATGCTAATTTACTGCAGAGAATGGATATGGATGCTGTTTTCTTCAGAGAGTTGGAGAATTCCCACAGGAAGGCATTTCTACTGGCAGACCATGCCTTGGCTGATGAAGACAGCGTGAGAAGTTCATGTGGAACAACAGCATTGACTGCTCTTATACTTGGAAGGCATTTGCTGGTTGCAAATGCTGGTGACTGTCGAGCAGTTCTTTGCAAGAAAGGAGTAGCAGTTGACTTGTCTCAAGATCACAAACCTAGTTACTTGCCAGAACGCATGCGAGTTGAGCAGTTGGGTGGTTACTTTGATGATGTGTATCTTAATGATTCTATCTCAGTCACCCGAGCCCTTGGAAATTGGGGCTTAAAATTACCATTTGGTTCCTCGTCGCCTCTAATTGCCGAGCCAGATGTTCAGCAGGTTATGTTAACAGAAGATGATGAGTTTTTGATCATTGGTTGTGATGGGATCTGGGATGTCATGTCAAGTCAGTATGCTGTGAGTCTGGTTCGGCGTGGGCTGAGGAGGCATGATGACCCCCAACTGTGTGCCGAAGAACTCGTCAAGGAAGCACTGCGCTTGAACACATCAGACAACCTCACAGTTATTGTCGTGCGCCTCTCTTCACCGGAACGTGTTGAGTTCCCTCACCAGCGCCCGAGGTTGAGGATCTGCAGTCTCTCGGAGGAAGCACGCAGCCGGCTGAGAAGCTTGTTAGAAGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

397

Amino Acids

43.79

Weight (kDa)

4.89

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 98 - 353 4.8e-61 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 871
AccB7I CCANNNNNTGG 2 cut(s) 19, 565
AciI CCGC 2 cut(s) 32, 395
AclWI GGATC 2 cut(s) 938, 1139
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 496
AcyI GRCGYC 2 cut(s) 75, 116
AfiI CCNNNNNNNGG 7 cut(s) 19, 52, 143, 354, 355, 538, 565
AflIII ACRYGT 1 cut(s) 1096
AgsI TTSAA 3 cut(s) 367, 467, 1045
AhdI GACNNNNNGTC 1 cut(s) 115
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 2 cut(s) 12, 45
AluBI AGCT 3 cut(s) 141, 428, 1173
AluI AGCT 3 cut(s) 141, 428, 1173
Alw21I GWGCWC 2 cut(s) 96, 324
Alw26I GTCTC 5 cut(s) 44, 158, 448, 708, 1145
AlwI GGATC 2 cut(s) 938, 1139
Ama87I CYCGRG 3 cut(s) 419, 804, 1120
AoxI GGCC 2 cut(s) 84, 416
ApaI GGGCCC 1 cut(s) 420
ApeKI GCWGC 3 cut(s) 425, 428, 1158
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 2 cut(s) 1008, 1040
AseI ATTAAT 1 cut(s) 222
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 3 cut(s) 1041, 1080, 1119
AspS9I GGNCC 3 cut(s) 296, 416, 417
AsuHPI GGTGA 4 cut(s) 668, 793, 1080, 1103
AsuII TTCGAA 1 cut(s) 192
AvaI CYCGRG 3 cut(s) 419, 804, 1120
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 420
BalI TGGCCA 1 cut(s) 86
BanII GRGCYC 3 cut(s) 361, 420, 811
BbsI GAAGAC 2 cut(s) 234, 582
Bbv12I GWGCWC 2 cut(s) 96, 324
BbvCI CCTCAGC 2 cut(s) 27, 983
BbvI GCAGC 3 cut(s) 415, 437, 1170
BccI CCATC 2 cut(s) 398, 920
BceAI ACGGC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciT130I CCWGG 2 cut(s) 14, 47
BclI TGATCA 1 cut(s) 912
BcoDI GTCTC 5 cut(s) 44, 158, 448, 708, 1145
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 4 cut(s) 101, 246, 482, 1135
BfoI RGCGCY 1 cut(s) 1120
BfuAI ACCTGC 1 cut(s) 871
BisI GCNGC 3 cut(s) 426, 429, 1159
BlsI GCNGC 3 cut(s) 427, 430, 1160
Bme1390I CCNGG 2 cut(s) 14, 47
Bme18I GGWCC 1 cut(s) 296
BmeRI GACNNNNNGTC 1 cut(s) 115
BmeT110I CYCGRG 3 cut(s) 419, 804, 1120
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 3 cut(s) 296, 416, 417
BmiI GGNNCC 2 cut(s) 418, 844
BmrFI CCNGG 2 cut(s) 14, 47
BmsI GCATC 2 cut(s) 460, 490
BpiI GAAGAC 2 cut(s) 234, 582
Bpu10I CCTNAGC 2 cut(s) 27, 983
Bpu14I TTCGAA 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 690
BsaBI GATNNNNATC 1 cut(s) 777
BsaHI GRCGYC 2 cut(s) 75, 116
BsaJI CCNNGG 7 cut(s) 87, 299, 309, 420, 564, 811, 1121
BsaWI WCCGGW 1 cut(s) 1090
BsaXI ACNNNNNCTCC 2 cut(s) 678, 708
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 52, 143, 354, 355, 538, 565
Bse118I RCCGGY 1 cut(s) 1161
Bse1I ACTGG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 777
BseBI CCWGG 2 cut(s) 14, 47
BseDI CCNNGG 7 cut(s) 87, 299, 309, 420, 564, 811, 1121
BseGI GGATG 3 cut(s) 319, 505, 943
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 7 cut(s) 19, 52, 143, 354, 355, 538, 565
BseMII CTCAG 6 cut(s) 18, 168, 194, 810, 974, 1157
BseNI ACTGG 1 cut(s) 555
BseRI GAGGAG 2 cut(s) 112, 1000
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 3 cut(s) 415, 437, 1170
BshFI GGCC 2 cut(s) 86, 418
BsiHKAI GWGCWC 2 cut(s) 96, 324
BsiHKCI CYCGRG 3 cut(s) 419, 804, 1120
BsiSI CCGG 2 cut(s) 1091, 1162
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 7 cut(s) 19, 52, 143, 354, 355, 538, 565
BsmAI GTCTC 5 cut(s) 44, 158, 448, 708, 1145
BsmBI CGTCTC 2 cut(s) 44, 158
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 2 cut(s) 86, 418
BsoBI CYCGRG 3 cut(s) 419, 804, 1120
Bsp119I TTCGAA 1 cut(s) 192
Bsp120I GGGCCC 1 cut(s) 416
Bsp1286I GDGCHC 5 cut(s) 96, 324, 361, 420, 811
Bsp143I GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 2 cut(s) 32, 395
BspANI GGCC 2 cut(s) 86, 418
BspCNI CTCAG 6 cut(s) 19, 169, 195, 809, 975, 1158
BspLI GGNNCC 2 cut(s) 418, 844
BspMAI CTGCAG 3 cut(s) 250, 486, 1139
BspMI ACCTGC 1 cut(s) 871
BspPI GGATC 2 cut(s) 938, 1139
BspT104I TTCGAA 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 1161
BsrI ACTGG 1 cut(s) 555
BssAI RCCGGY 1 cut(s) 1161
BssECI CCNNGG 7 cut(s) 87, 299, 309, 420, 564, 811, 1121
BssMI GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
BssNI GRCGYC 2 cut(s) 75, 116
BssT1I CCWWGG 5 cut(s) 87, 299, 309, 564, 811
Bst2UI CCWGG 2 cut(s) 14, 47
Bst4CI ACNGT 4 cut(s) 55, 662, 1008, 1066
Bst6I CTCTTC 2 cut(s) 368, 1090
BstACI GRCGYC 2 cut(s) 75, 116
BstAPI GCANNNNNTGC 3 cut(s) 392, 560, 614
BstBI TTCGAA 1 cut(s) 192
BstC8I GCNNGC 3 cut(s) 740, 1156, 1163
BstDEI CTNAG 6 cut(s) 27, 177, 203, 796, 983, 1166
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 3 cut(s) 319, 505, 943
BstH2I RGCGCY 1 cut(s) 1120
BstHHI GCGC 3 cut(s) 1041, 1080, 1119
BstKTI GATC 6 cut(s) 108, 340, 712, 915, 933, 1134
BstMAI GTCTC 5 cut(s) 44, 158, 448, 708, 1145
BstMBI GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
BstMWI GCNNNNNNNGC 5 cut(s) 392, 560, 614, 748, 988
BstNI CCWGG 2 cut(s) 14, 47
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 2 cut(s) 12, 45
BstSFI CTRYAG 4 cut(s) 101, 246, 482, 1135
BstSLI GKGCMC 1 cut(s) 420
BstV1I GCAGC 3 cut(s) 415, 437, 1170
BstV2I GAAGAC 2 cut(s) 234, 582
BstX2I RGATCY 2 cut(s) 930, 1131
BstYI RGATCY 2 cut(s) 930, 1131
BsuRI GGCC 2 cut(s) 86, 418
BtgI CCRYGG 1 cut(s) 309
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 3 cut(s) 319, 505, 943
BtsI GCAGTG 1 cut(s) 1034
BtsIMutI CAGTG 1 cut(s) 1034
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 3 cut(s) 740, 1156, 1163
CfoI GCGC 3 cut(s) 1041, 1080, 1119
Cfr10I RCCGGY 1 cut(s) 1161
Cfr13I GGNCC 3 cut(s) 296, 416, 417
CseI GACGC 2 cut(s) 64, 105
CviAII CATG 8 cut(s) 64, 310, 350, 560, 594, 739, 943, 992
DdeI CTNAG 6 cut(s) 27, 177, 203, 796, 983, 1166
DpnI GATC 6 cut(s) 107, 339, 711, 914, 932, 1133
DpnII GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
DriI GACNNNNNGTC 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 368, 1090
Eam1105I GACNNNNNGTC 1 cut(s) 115
EarI CTCTTC 2 cut(s) 368, 1090
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 5 cut(s) 87, 299, 309, 564, 811
Eco24I GRGCYC 3 cut(s) 361, 420, 811
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 496
Eco88I CYCGRG 3 cut(s) 419, 804, 1120
EcoO109I RGGNCCY 1 cut(s) 416
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 12, 45
EcoT14I CCWWGG 5 cut(s) 87, 299, 309, 564, 811
EcoT38I GRGCYC 3 cut(s) 361, 420, 811
ErhI CCWWGG 5 cut(s) 87, 299, 309, 564, 811
Esp3I CGTCTC 2 cut(s) 44, 158
FaeI CATG 8 cut(s) 67, 313, 353, 563, 597, 742, 946, 995
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 1 cut(s) 357
FatI CATG 8 cut(s) 63, 309, 349, 559, 593, 738, 942, 991
FbaI TGATCA 1 cut(s) 912
Fnu4HI GCNGC 3 cut(s) 426, 429, 1159
FokI GGATG 3 cut(s) 326, 512, 950
FriOI GRGCYC 3 cut(s) 361, 420, 811
Fsp4HI GCNGC 3 cut(s) 426, 429, 1159
FspBI CTAG 1 cut(s) 720
GlaI GCGC 3 cut(s) 1040, 1079, 1118
GluI GCNGC 3 cut(s) 426, 429, 1159
HaeII RGCGCY 1 cut(s) 1120
HaeIII GGCC 2 cut(s) 86, 418
HapII CCGG 2 cut(s) 1091, 1162
HgaI GACGC 2 cut(s) 64, 105
HhaI GCGC 3 cut(s) 1041, 1080, 1119
Hin1I GRCGYC 2 cut(s) 75, 116
Hin1II CATG 8 cut(s) 67, 313, 353, 563, 597, 742, 946, 995
Hin6I GCGC 3 cut(s) 1039, 1078, 1117
HinP1I GCGC 3 cut(s) 1039, 1078, 1117
HincII GTYRAC 2 cut(s) 697, 891
HindII GTYRAC 2 cut(s) 697, 891
HindIII AAGCTT 1 cut(s) 1171
HinfI GANTC 5 cut(s) 194, 239, 305, 788, 964
HpaI GTTAAC 1 cut(s) 891
HpaII CCGG 2 cut(s) 1091, 1162
HphI GGTGA 4 cut(s) 668, 793, 1080, 1103
Hpy166II GTNNAC 2 cut(s) 697, 891
Hpy188I TCNGA 7 cut(s) 138, 204, 264, 330, 515, 1054, 1147
Hpy188III TCNNGA 3 cut(s) 276, 439, 707
Hpy8I GTNNAC 2 cut(s) 697, 891
Hpy99I CGWCG 3 cut(s) 113, 161, 853
HpyAV CCTTC 3 cut(s) 532, 624, 1175
HpyCH4III ACNGT 4 cut(s) 55, 662, 1008, 1066
HpyCH4IV ACGT 2 cut(s) 111, 1096
HpyCH4V TGCA 7 cut(s) 210, 248, 386, 484, 647, 678, 1137
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 560, 614, 748, 988
HpyF3I CTNAG 6 cut(s) 27, 177, 203, 796, 983, 1166
HpySE526I ACGT 2 cut(s) 111, 1096
Hsp92I GRCGYC 2 cut(s) 75, 116
Hsp92II CATG 8 cut(s) 67, 313, 353, 563, 597, 742, 946, 995
HspAI GCGC 3 cut(s) 1039, 1078, 1117
KroI GCCGGC 1 cut(s) 1161
KroNI GCCGGC 1 cut(s) 1163
Ksp22I TGATCA 1 cut(s) 912
KspAI GTTAAC 1 cut(s) 891
Kzo9I GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
LmnI GCTCC 1 cut(s) 91
Lsp1109I GCAGC 3 cut(s) 415, 437, 1170
LweI GCATC 2 cut(s) 460, 490
MaeI CTAG 1 cut(s) 720
MaeII ACGT 2 cut(s) 111, 1096
MaeIII GTNAC 6 cut(s) 287, 407, 656, 722, 761, 799
MalI GATC 6 cut(s) 107, 339, 711, 914, 932, 1133
MboI GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
MboII GAAGA 8 cut(s) 234, 355, 479, 502, 587, 908, 1028, 1077
MfeI CAATTG 1 cut(s) 1186
MflI RGATCY 2 cut(s) 930, 1131
MhlI GDGCHC 5 cut(s) 96, 324, 361, 420, 811
MlsI TGGCCA 1 cut(s) 86
MluCI AATT 6 cut(s) 475, 526, 817, 830, 858, 1186
MluNI TGGCCA 1 cut(s) 86
MlyI GAGTC 2 cut(s) 248, 973
MmeI TCCRAC 1 cut(s) 501
Mox20I TGGCCA 1 cut(s) 86
MroNI GCCGGC 1 cut(s) 1161
MroXI GAANNNNTTC 1 cut(s) 198
MscI TGGCCA 1 cut(s) 86
MseI TTAA 4 cut(s) 222, 783, 827, 890
Msp20I TGGCCA 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 2 cut(s) 1091, 1162
MspR9I CCNGG 2 cut(s) 14, 47
MunI CAATTG 1 cut(s) 1186
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 2 cut(s) 14, 47
MwoI GCNNNNNNNGC 5 cut(s) 392, 560, 614, 748, 988
NaeI GCCGGC 1 cut(s) 1163
NcoI CCATGG 1 cut(s) 309
NdeII GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
NgoMIV GCCGGC 1 cut(s) 1161
NlaIII CATG 8 cut(s) 67, 313, 353, 563, 597, 742, 946, 995
NlaIV GGNNCC 2 cut(s) 418, 844
NmeAIII GCCGAG 2 cut(s) 187, 889
NmuCI GTSAC 4 cut(s) 287, 407, 656, 799
NspI RCATGY 1 cut(s) 742
NspV TTCGAA 1 cut(s) 192
PaeI GCATGC 1 cut(s) 742
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 417
PctI GAATGC 1 cut(s) 451
PdiI GCCGGC 1 cut(s) 1163
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 3 cut(s) 194, 305, 788
PflMI CCANNNNNTGG 2 cut(s) 19, 565
PkrI GCNGC 3 cut(s) 427, 430, 1160
PleI GAGTC 2 cut(s) 247, 972
PpsI GAGTC 2 cut(s) 247, 972
PshBI ATTAAT 1 cut(s) 222
Psp6I CCWGG 2 cut(s) 12, 45
PspGI CCWGG 2 cut(s) 12, 45
PspN4I GGNNCC 2 cut(s) 418, 844
PspOMI GGGCCC 1 cut(s) 416
PspPI GGNCC 3 cut(s) 296, 416, 417
PstI CTGCAG 3 cut(s) 250, 486, 1139
PsuI RGATCY 2 cut(s) 930, 1131
PvuII CAGCTG 1 cut(s) 428
SaqAI TTAA 4 cut(s) 222, 783, 827, 890
SatI GCNGC 3 cut(s) 426, 429, 1159
Sau3AI GATC 6 cut(s) 105, 337, 709, 912, 930, 1131
Sau96I GGNCC 3 cut(s) 296, 416, 417
SchI GAGTC 2 cut(s) 248, 973
ScrFI CCNGG 2 cut(s) 14, 47
SduI GDGCHC 5 cut(s) 96, 324, 361, 420, 811
SfaNI GCATC 2 cut(s) 460, 490
SfcI CTRYAG 4 cut(s) 101, 246, 482, 1135
SfuI TTCGAA 1 cut(s) 192
SinI GGWCC 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
SphI GCATGC 1 cut(s) 742
Sse9I AATT 6 cut(s) 475, 526, 817, 830, 858, 1186
SsiI CCGC 2 cut(s) 32, 395
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 2 cut(s) 12, 45
StyI CCWWGG 5 cut(s) 87, 299, 309, 564, 811
TaaI ACNGT 4 cut(s) 55, 662, 1008, 1066
TaiI ACGT 2 cut(s) 114, 1099
TaqI TCGA 3 cut(s) 108, 192, 664
TasI AATT 6 cut(s) 475, 526, 817, 830, 858, 1186
TfiI GAWTC 3 cut(s) 194, 305, 788
Tru1I TTAA 4 cut(s) 222, 783, 827, 890
Tru9I TTAA 4 cut(s) 222, 783, 827, 890
TscAI CASTG 1 cut(s) 1041
TseFI GTSAC 4 cut(s) 287, 407, 656, 799
TseI GCWGC 3 cut(s) 425, 428, 1158
Tsp45I GTSAC 4 cut(s) 287, 407, 656, 799
TspDTI ATGAA 4 cut(s) 80, 260, 582, 588
TspGWI ACGGA 1 cut(s) 426
TspRI CASTG 1 cut(s) 1041
Van91I CCANNNNNTGG 2 cut(s) 19, 565
VpaK11BI GGWCC 1 cut(s) 296
VspI ATTAAT 1 cut(s) 222
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.