Rh4BG273200

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015600
Physical Location & Seq
Reverse (-)
44731659 .. 44734223
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG273200.1

Sequence Viewer

Length: 1050 bp
ATGATTCTGAGCCAGAATATGGTGGCTGAGGCAGAGATTTCGACCGAGGTTTCGGGCTTCGAATCGGTTGTGAGGTGCTCAGAGACCATTGCTAATGCTGTAATAGAGACTGCTGCAGTCAAGTTTGTTCCAACCATTCACTCAGGAAGCCATAGTGACATCGGAGAAAGGCGTTCCATGGATGACGATCACATTCGGATCGATGACCTATCTGCCCGTGTTGGGCCTTTCTTTAATAGTTCCTTGCCAAGTGCATTTTATGCAGTTTTTGATGGGCATGACGGGCCTGCAGCAGCTACTTATATCAAGAGGAATGCCATGAGACTATTTTTTGAAGATGCTGATTTGCCGCATACAGTAGATTTTGATGATGCTTTCTTCAAAGAGTTGGAGAACTCACATAAGAAAGCATTTTTACTGGCAGACCAGGCCTTGGCCGATGAACACAGTGTGGATGGTTCATGTGGAACCACAGCATTGACTGCCCTAGTACTTGGAAGGCAGTTGCTTGTAGCAAATGCTGGTGACTGTCGGGCAGTTCTTTGCAGGAAAGGAATCGCATTTGATATGTCCCAAGATCATAAGCCTACTTACTTGCCGGAACGTAGGCGGGTGGAGCAGTTGGGTGGTTACATTGATGATGGGTATCTGAATGGCTATCTTTCAGTCACCCGAACCCTTGGAAATTGGGATTTCAAATTACCACTTGGCTCCTCATCACCTCTCATTGCTGAACCGGAGGTTCAGCGGGTTAAATTAACAGAAGATGATGAGTTTCTGATTCTTAGTTGTGATGGGATATGGGATGTCATGTCTAGCCAATACGCAGTGAGTCTTGTTCGGCGTGAGCTGAGGAAGCATAATGATCCGCAACAGTGTGCCAGAGAACTCGTGAAAGAAGCACTACGCAAGCATGCATCTGACAATCTCACTGTTATTGTTGTATGCCTCTCTTCTCCTGATTGTGCTGTTGAGCCTCGCTCTGAGGGGCCAAGGTTAAGGAACTGCAGTTTTTCAAGGTTGAGAAGCTTGCTGGAAGGCGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

349

Amino Acids

38.56

Weight (kDa)

5.13

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 50 - 309 8.2e-64 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 19, 433
AciI CCGC 5 cut(s) 350, 610, 748, 869, 1041
AclWI GGATC 2 cut(s) 206, 860
AcoI YGGCCR 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 451
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 3 cut(s) 19, 222, 433
AgsI TTSAA 4 cut(s) 335, 382, 697, 1017
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 3 cut(s) 296, 850, 1029
AluI AGCT 3 cut(s) 296, 850, 1029
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 3 cut(s) 77, 101, 316
AlwI GGATC 2 cut(s) 206, 860
AoxI GGCC 5 cut(s) 224, 284, 429, 435, 989
ApeKI GCWGC 3 cut(s) 113, 290, 293
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AspS9I GGNCC 3 cut(s) 224, 284, 989
AsuHPI GGTGA 3 cut(s) 536, 661, 711
AsuII TTCGAA 1 cut(s) 60
BauI CACGAG 1 cut(s) 890
Bbv12I GWGCWC 1 cut(s) 80
BbvCI CCTCAGC 2 cut(s) 27, 851
BbvI GCAGC 3 cut(s) 100, 302, 305
BccI CCATC 4 cut(s) 266, 449, 635, 788
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 1 cut(s) 428
BcoDI GTCTC 3 cut(s) 77, 101, 316
BfaI CTAG 2 cut(s) 488, 816
BfmI CTRYAG 3 cut(s) 114, 288, 1006
BisI GCNGC 5 cut(s) 114, 291, 294, 350, 1042
BlsI GCNGC 5 cut(s) 115, 292, 295, 351, 1043
BmcAI AGTACT 1 cut(s) 492
Bme1390I CCNGG 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 224, 284, 989
BmiI GGNNCC 3 cut(s) 469, 712, 990
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 3 cut(s) 328, 361, 926
BplI GAGNNNNNCTC 2 cut(s) 965, 997
Bpu10I CCTNAGC 2 cut(s) 27, 851
Bpu14I TTCGAA 1 cut(s) 60
Bsa29I ATCGAT 1 cut(s) 201
BsaBI GATNNNNATC 2 cut(s) 186, 645
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 5 cut(s) 45, 177, 432, 679, 992
BsaWI WCCGGW 1 cut(s) 736
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 222, 433
Bse1I ACTGG 1 cut(s) 423
Bse3DI GCAATG 2 cut(s) 87, 726
Bse8I GATNNNNATC 2 cut(s) 186, 645
BseBI CCWGG 1 cut(s) 428
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 5 cut(s) 45, 177, 432, 679, 992
BseGI GGATG 3 cut(s) 187, 460, 811
BseJI GATNNNNATC 2 cut(s) 186, 645
BseLI CCNNNNNNNGG 3 cut(s) 19, 222, 433
BseMI GCAATG 2 cut(s) 87, 726
BseMII CTCAG 5 cut(s) 18, 93, 156, 842, 975
BseNI ACTGG 1 cut(s) 423
BseRI GAGGAG 1 cut(s) 703
BseXI GCAGC 3 cut(s) 100, 302, 305
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 226, 286, 431, 437, 991
BshVI ATCGAT 1 cut(s) 201
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 80
BsiSI CCGG 2 cut(s) 599, 737
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 3 cut(s) 19, 222, 433
BsmAI GTCTC 3 cut(s) 77, 101, 316
BsmFI GGGAC 1 cut(s) 556
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 5 cut(s) 226, 286, 431, 437, 991
Bso31I GGTCTC 1 cut(s) 77
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 4 cut(s) 187, 198, 577, 865
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 5 cut(s) 350, 610, 748, 869, 1041
BspANI GGCC 5 cut(s) 226, 286, 431, 437, 991
BspCNI CTCAG 5 cut(s) 19, 92, 155, 843, 976
BspDI ATCGAT 1 cut(s) 201
BspLI GGNNCC 3 cut(s) 469, 712, 990
BspMAI CTGCAG 3 cut(s) 118, 292, 1010
BspPI GGATC 2 cut(s) 206, 860
BspT104I TTCGAA 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 2 cut(s) 87, 726
BsrI ACTGG 1 cut(s) 423
BssECI CCNNGG 5 cut(s) 45, 177, 432, 679, 992
BssMI GATC 4 cut(s) 187, 198, 577, 865
BssSI CACGAG 1 cut(s) 890
BssT1I CCWWGG 4 cut(s) 177, 432, 679, 992
Bst2BI CACGAG 1 cut(s) 890
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 5 cut(s) 358, 449, 530, 876, 934
Bst6I CTCTTC 1 cut(s) 958
BstAPI GCANNNNNTGC 2 cut(s) 260, 482
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 4 cut(s) 288, 911, 915, 1031
BstDEI CTNAG 7 cut(s) 8, 27, 79, 142, 785, 851, 984
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 187, 460, 811
BstKTI GATC 4 cut(s) 190, 201, 580, 868
BstMAI GTCTC 3 cut(s) 77, 101, 316
BstMBI GATC 4 cut(s) 187, 198, 577, 865
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 6 cut(s) 260, 283, 428, 482, 616, 856
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 917
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 3 cut(s) 114, 288, 1006
BstV1I GCAGC 3 cut(s) 100, 302, 305
Bsu15I ATCGAT 1 cut(s) 201
BsuRI GGCC 5 cut(s) 226, 286, 431, 437, 991
BsuTUI ATCGAT 1 cut(s) 201
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 187, 460, 811
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 4 cut(s) 454, 834, 881, 930
Cac8I GCNNGC 4 cut(s) 288, 911, 915, 1031
Cfr13I GGNCC 3 cut(s) 224, 284, 989
ClaI ATCGAT 1 cut(s) 201
Csp6I GTAC 1 cut(s) 491
CviAII CATG 6 cut(s) 178, 278, 319, 462, 811, 914
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 7 cut(s) 8, 27, 79, 142, 785, 851, 984
DpnI GATC 4 cut(s) 189, 200, 579, 867
DpnII GATC 4 cut(s) 187, 198, 577, 865
DraIII CACNNNGTG 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 958
EarI CTCTTC 1 cut(s) 958
Eco130I CCWWGG 4 cut(s) 177, 432, 679, 992
Eco147I AGGCCT 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 426
EcoT14I CCWWGG 4 cut(s) 177, 432, 679, 992
EcoT22I ATGCAT 1 cut(s) 919
ErhI CCWWGG 4 cut(s) 177, 432, 679, 992
FaeI CATG 6 cut(s) 181, 281, 322, 465, 814, 917
FaqI GGGAC 1 cut(s) 556
FatI CATG 6 cut(s) 177, 277, 318, 461, 810, 913
FauI CCCGC 2 cut(s) 603, 741
Fnu4HI GCNGC 5 cut(s) 114, 291, 294, 350, 1042
FokI GGATG 3 cut(s) 194, 467, 818
Fsp4HI GCNGC 5 cut(s) 114, 291, 294, 350, 1042
FspBI CTAG 2 cut(s) 488, 816
GluI GCNGC 5 cut(s) 114, 291, 294, 350, 1042
HaeIII GGCC 5 cut(s) 226, 286, 431, 437, 991
HapII CCGG 2 cut(s) 599, 737
Hin1II CATG 6 cut(s) 181, 281, 322, 465, 814, 917
HindIII AAGCTT 1 cut(s) 1027
HinfI GANTC 5 cut(s) 4, 62, 555, 781, 832
HpaII CCGG 2 cut(s) 599, 737
HphI GGTGA 3 cut(s) 536, 661, 711
Hpy188I TCNGA 8 cut(s) 9, 82, 164, 198, 651, 780, 922, 985
Hpy188III TCNNGA 4 cut(s) 144, 307, 892, 959
HpyAV CCTTC 2 cut(s) 492, 1031
HpyCH4III ACNGT 5 cut(s) 358, 449, 530, 876, 934
HpyCH4IV ACGT 1 cut(s) 604
HpyCH4V TGCA 7 cut(s) 116, 254, 263, 290, 546, 917, 1008
HpyF10VI GCNNNNNNNGC 6 cut(s) 260, 283, 428, 482, 616, 856
HpyF3I CTNAG 7 cut(s) 8, 27, 79, 142, 785, 851, 984
HpySE526I ACGT 1 cut(s) 604
Hsp92II CATG 6 cut(s) 181, 281, 322, 465, 814, 917
Kzo9I GATC 4 cut(s) 187, 198, 577, 865
LmnI GCTCC 2 cut(s) 616, 716
Lsp1109I GCAGC 3 cut(s) 100, 302, 305
LweI GCATC 3 cut(s) 328, 361, 926
MaeI CTAG 2 cut(s) 488, 816
MaeII ACGT 1 cut(s) 604
MaeIII GTNAC 4 cut(s) 155, 524, 629, 667
MalI GATC 4 cut(s) 189, 200, 579, 867
MboI GATC 4 cut(s) 187, 198, 577, 865
MboII GAAGA 4 cut(s) 347, 370, 776, 945
MfeI CAATTG 1 cut(s) 1045
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 4 cut(s) 685, 698, 755, 1045
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 2 cut(s) 155, 369
Mph1103I ATGCAT 1 cut(s) 919
MseI TTAA 4 cut(s) 234, 753, 758, 998
MspA1I CMGCKG 1 cut(s) 748
MspI CCGG 2 cut(s) 599, 737
MspR9I CCNGG 1 cut(s) 428
MunI CAATTG 1 cut(s) 1045
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 6 cut(s) 260, 283, 428, 482, 616, 856
NcoI CCATGG 1 cut(s) 177
NdeII GATC 4 cut(s) 187, 198, 577, 865
NlaIII CATG 6 cut(s) 181, 281, 322, 465, 814, 917
NlaIV GGNNCC 3 cut(s) 469, 712, 990
NmuCI GTSAC 3 cut(s) 155, 524, 667
NsiI ATGCAT 1 cut(s) 919
NspI RCATGY 1 cut(s) 917
NspV TTCGAA 1 cut(s) 60
PaeI GCATGC 1 cut(s) 917
PceI AGGCCT 1 cut(s) 431
PctI GAATGC 1 cut(s) 319
PfeI GAWTC 4 cut(s) 4, 62, 555, 781
PflMI CCANNNNNTGG 2 cut(s) 19, 433
PkrI GCNGC 5 cut(s) 115, 292, 295, 351, 1043
PleI GAGTC 1 cut(s) 840
PpsI GAGTC 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 3 cut(s) 469, 712, 990
PspPI GGNCC 3 cut(s) 224, 284, 989
PstI CTGCAG 3 cut(s) 118, 292, 1010
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
SaqAI TTAA 4 cut(s) 234, 753, 758, 998
SatI GCNGC 5 cut(s) 114, 291, 294, 350, 1042
Sau3AI GATC 4 cut(s) 187, 198, 577, 865
Sau96I GGNCC 3 cut(s) 224, 284, 989
ScaI AGTACT 1 cut(s) 492
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 3 cut(s) 328, 361, 926
SfcI CTRYAG 3 cut(s) 114, 288, 1006
SfuI TTCGAA 1 cut(s) 60
SphI GCATGC 1 cut(s) 917
Sse9I AATT 4 cut(s) 685, 698, 755, 1045
SseBI AGGCCT 1 cut(s) 431
SsiI CCGC 5 cut(s) 350, 610, 748, 869, 1041
SspMI CTAG 2 cut(s) 488, 816
StuI AGGCCT 1 cut(s) 431
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 4 cut(s) 177, 432, 679, 992
TaaI ACNGT 5 cut(s) 358, 449, 530, 876, 934
TaiI ACGT 1 cut(s) 607
TaqI TCGA 3 cut(s) 41, 60, 201
TaqII GACCGA 1 cut(s) 59
TasI AATT 4 cut(s) 685, 698, 755, 1045
TatI WGTACW 1 cut(s) 490
TauI GCSGC 2 cut(s) 352, 1044
TfiI GAWTC 4 cut(s) 4, 62, 555, 781
Tru1I TTAA 4 cut(s) 234, 753, 758, 998
Tru9I TTAA 4 cut(s) 234, 753, 758, 998
TscAI CASTG 4 cut(s) 454, 834, 881, 937
TseFI GTSAC 3 cut(s) 155, 524, 667
TseI GCWGC 3 cut(s) 113, 290, 293
Tsp45I GTSAC 3 cut(s) 155, 524, 667
TspDTI ATGAA 2 cut(s) 450, 456
TspRI CASTG 4 cut(s) 454, 834, 881, 937
Van91I CCANNNNNTGG 2 cut(s) 19, 433
XceI RCATGY 1 cut(s) 917
XspI CTAG 2 cut(s) 488, 816
ZrmI AGTACT 1 cut(s) 492
Zsp2I ATGCAT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.