Rh4CG287400

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015600
Physical Location & Seq
Reverse (-)
54509045 .. 54511660
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG287400.1

Sequence Viewer

Length: 1065 bp
ATGATTGTGAGCCAGAATATGGTGGCTGAGGCCGAGATTATTTGCCAGAATATGGTGTCGACCGAGGTTTCGGGCTTCGAATCGGTTGTGAGGTGCCCAGAGACCATTGCTAATGCTGTAATAGAGACTCCTGCAGTCAAGTTTGTTCCAACCATTCACTCAGGAAGCCATAGTGACATCGGAGTAAGGCGTTCCATGGATGACGATCACATTCGGATTGATGACCTATCTGCCCATGTTGGGCCTCTCTTTAAGAGTTCCTTGCCAAGTGCATTTTATGCAGTTTTTGATGGGCATGGCGGGCCTGAAGCAGCTACTTATATCAAGAGGAATGCCATGAGACTATTTTTTGAAGATGCTGACTTGCCGCATACAGTAGATTTTGATGATGCTTTCTTCAAAGAGTTGGAGAACTCACATAAGAAAGCATTTTTACTGGCAGACCAGGCCTTGGCCGATGAACACAGTGTGGATGGTTCATGTGGAACCACAGCACTGACTGCCCTAGTACTTGGAAGGCAGTTGCTCGTAGCAAATGCTGGTGACTGTCGGGCAGTTCTTTGCAGGAAAGGAATCGCATTTGATATGTCCCAAGATCATAAGCATACTTACTTGCCAGAACGTAGGCGGGTGGAGCAGTTGGGTGGTTACTTTGATGGTGAGTATCTGAATAGTTATCTCTCAGTCACCCGAACCCTTGGAAATTGGGATTTCAAATTATCACTTGGCTCCTCATCACCTCTCATTGCTGAACCGGAGGTTCAGCGGGTTAAGTTAACAGAAGATGATGAGTTTCTGATTCTTAGTTGTGATGGGATATGGGATGTCATGTCTAGCCAATATGCAGTGAGTCTTGTTCGGCGTGAGCTGAGGAAGCACAATGATCCGCAACAGTGTGCCAGAGAACTCGTGAAAGAAGCACTACGCAAGCATGCAGCTGACAATCTCACTGTTATTGTTGTATGCCTCTCTTCTCCTGATCGTGCTGTTGAGCCTCGCTCTGAGGGGCCAAGGTTAAGGAACTGCAGTTTTTCAAGGTTGAGAAGCTTGCTGGAAGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

354

Amino Acids

39.29

Weight (kDa)

5.41

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 55 - 315 7e-63 Protein phosphatase 2C
PP2C_2 PF13672 98 - 304 3e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 93
AccB7I CCANNNNNTGG 3 cut(s) 19, 52, 451
AccI GTMKAC 1 cut(s) 59
AciI CCGC 5 cut(s) 300, 368, 628, 766, 887
AclWI GGATC 1 cut(s) 878
AcoI YGGCCR 1 cut(s) 453
AcuI CTGAAG 1 cut(s) 327
AdeI CACNNNGTG 1 cut(s) 469
AfaI GTAC 1 cut(s) 510
AfiI CCNNNNNNNGG 4 cut(s) 19, 52, 240, 451
AgsI TTSAA 4 cut(s) 353, 400, 715, 1035
AjnI CCWGG 1 cut(s) 444
AluBI AGCT 4 cut(s) 314, 868, 938, 1047
AluI AGCT 4 cut(s) 314, 868, 938, 1047
Alw26I GTCTC 3 cut(s) 95, 119, 334
AlwI GGATC 1 cut(s) 878
AoxI GGCC 6 cut(s) 30, 242, 302, 447, 453, 1007
ApeKI GCWGC 2 cut(s) 311, 935
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AspS9I GGNCC 3 cut(s) 242, 302, 1007
AsuHPI GGTGA 4 cut(s) 554, 671, 679, 729
AsuII TTCGAA 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 98
BanI GGYRCC 1 cut(s) 93
BauI CACGAG 1 cut(s) 908
BbvCI CCTCAGC 2 cut(s) 27, 869
BbvI GCAGC 2 cut(s) 323, 947
BccI CCATC 4 cut(s) 284, 467, 650, 806
BciT130I CCWGG 1 cut(s) 446
BcoDI GTCTC 3 cut(s) 95, 119, 334
BfaI CTAG 2 cut(s) 506, 834
BfmI CTRYAG 2 cut(s) 132, 1024
BisI GCNGC 3 cut(s) 312, 368, 936
BlsI GCNGC 3 cut(s) 313, 369, 937
BmcAI AGTACT 1 cut(s) 510
Bme1390I CCNGG 1 cut(s) 446
BmgT120I GGNCC 3 cut(s) 242, 302, 1007
BmiI GGNNCC 4 cut(s) 95, 487, 730, 1008
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 2 cut(s) 346, 379
BplI GAGNNNNNCTC 2 cut(s) 983, 1015
Bpu10I CCTNAGC 2 cut(s) 27, 869
Bpu14I TTCGAA 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 5 cut(s) 63, 195, 450, 697, 1010
BsaWI WCCGGW 1 cut(s) 754
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 52, 240, 451
Bse1I ACTGG 1 cut(s) 441
Bse3DI GCAATG 2 cut(s) 105, 744
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 5 cut(s) 63, 195, 450, 697, 1010
BseGI GGATG 3 cut(s) 205, 478, 829
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 4 cut(s) 19, 52, 240, 451
BseMI GCAATG 2 cut(s) 105, 744
BseMII CTCAG 5 cut(s) 18, 174, 696, 860, 993
BseNI ACTGG 1 cut(s) 441
BseRI GAGGAG 1 cut(s) 721
BseSI GKGCMC 1 cut(s) 98
BseXI GCAGC 2 cut(s) 323, 947
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 6 cut(s) 32, 244, 304, 449, 455, 1009
BshNI GGYRCC 1 cut(s) 93
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 1 cut(s) 755
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 4 cut(s) 19, 52, 240, 451
BsmAI GTCTC 3 cut(s) 95, 119, 334
BsmFI GGGAC 1 cut(s) 574
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 6 cut(s) 32, 244, 304, 449, 455, 1009
Bso31I GGTCTC 1 cut(s) 95
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 4 cut(s) 205, 595, 883, 979
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 5 cut(s) 300, 368, 628, 766, 887
BspANI GGCC 6 cut(s) 32, 244, 304, 449, 455, 1009
BspCNI CTCAG 5 cut(s) 19, 173, 695, 861, 994
BspLI GGNNCC 4 cut(s) 95, 487, 730, 1008
BspMAI CTGCAG 2 cut(s) 136, 1028
BspPI GGATC 1 cut(s) 878
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 93
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 2 cut(s) 105, 744
BsrI ACTGG 1 cut(s) 441
BssECI CCNNGG 5 cut(s) 63, 195, 450, 697, 1010
BssMI GATC 4 cut(s) 205, 595, 883, 979
BssSI CACGAG 1 cut(s) 908
BssT1I CCWWGG 4 cut(s) 195, 450, 697, 1010
Bst2BI CACGAG 1 cut(s) 908
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 5 cut(s) 376, 467, 548, 894, 952
Bst6I CTCTTC 1 cut(s) 976
BstAPI GCANNNNNTGC 2 cut(s) 278, 500
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 4 cut(s) 302, 929, 933, 1049
BstDEI CTNAG 6 cut(s) 27, 160, 682, 803, 869, 1002
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 3 cut(s) 205, 478, 829
BstKTI GATC 4 cut(s) 208, 598, 886, 982
BstMAI GTCTC 3 cut(s) 95, 119, 334
BstMBI GATC 4 cut(s) 205, 595, 883, 979
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 6 cut(s) 278, 301, 446, 500, 634, 874
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 1 cut(s) 935
BstSCI CCNGG 1 cut(s) 444
BstSFI CTRYAG 2 cut(s) 132, 1024
BstSLI GKGCMC 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 323, 947
BsuRI GGCC 6 cut(s) 32, 244, 304, 449, 455, 1009
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 3 cut(s) 205, 478, 829
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 5 cut(s) 472, 494, 852, 899, 948
Cac8I GCNNGC 4 cut(s) 302, 929, 933, 1049
Cfr13I GGNCC 3 cut(s) 242, 302, 1007
Csp6I GTAC 1 cut(s) 509
CviAII CATG 7 cut(s) 196, 236, 296, 337, 480, 829, 932
CviQI GTAC 1 cut(s) 509
DdeI CTNAG 6 cut(s) 27, 160, 682, 803, 869, 1002
DpnI GATC 4 cut(s) 207, 597, 885, 981
DpnII GATC 4 cut(s) 205, 595, 883, 979
DraIII CACNNNGTG 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 976
EarI CTCTTC 1 cut(s) 976
Eco130I CCWWGG 4 cut(s) 195, 450, 697, 1010
Eco147I AGGCCT 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 327
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 4 cut(s) 195, 450, 697, 1010
ErhI CCWWGG 4 cut(s) 195, 450, 697, 1010
FaeI CATG 7 cut(s) 199, 239, 299, 340, 483, 832, 935
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 574
FatI CATG 7 cut(s) 195, 235, 295, 336, 479, 828, 931
FauI CCCGC 3 cut(s) 293, 621, 759
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 3 cut(s) 312, 368, 936
FokI GGATG 3 cut(s) 212, 485, 836
Fsp4HI GCNGC 3 cut(s) 312, 368, 936
FspBI CTAG 2 cut(s) 506, 834
GluI GCNGC 3 cut(s) 312, 368, 936
HaeIII GGCC 6 cut(s) 32, 244, 304, 449, 455, 1009
HapII CCGG 1 cut(s) 755
Hin1II CATG 7 cut(s) 199, 239, 299, 340, 483, 832, 935
HincII GTYRAC 2 cut(s) 60, 777
HindII GTYRAC 2 cut(s) 60, 777
HindIII AAGCTT 1 cut(s) 1045
HinfI GANTC 5 cut(s) 80, 127, 573, 799, 850
HpaI GTTAAC 1 cut(s) 777
HpaII CCGG 1 cut(s) 755
HphI GGTGA 4 cut(s) 554, 671, 679, 729
Hpy166II GTNNAC 2 cut(s) 60, 777
Hpy188I TCNGA 5 cut(s) 182, 216, 669, 798, 1003
Hpy188III TCNNGA 4 cut(s) 162, 325, 910, 977
Hpy8I GTNNAC 2 cut(s) 60, 777
HpyAV CCTTC 2 cut(s) 510, 1049
HpyCH4III ACNGT 5 cut(s) 376, 467, 548, 894, 952
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 7 cut(s) 134, 272, 281, 564, 845, 935, 1026
HpyF10VI GCNNNNNNNGC 6 cut(s) 278, 301, 446, 500, 634, 874
HpyF3I CTNAG 6 cut(s) 27, 160, 682, 803, 869, 1002
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 7 cut(s) 199, 239, 299, 340, 483, 832, 935
KspAI GTTAAC 1 cut(s) 777
Kzo9I GATC 4 cut(s) 205, 595, 883, 979
LmnI GCTCC 2 cut(s) 634, 734
Lsp1109I GCAGC 2 cut(s) 323, 947
LweI GCATC 2 cut(s) 346, 379
MaeI CTAG 2 cut(s) 506, 834
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 4 cut(s) 173, 542, 647, 685
MalI GATC 4 cut(s) 207, 597, 885, 981
MboI GATC 4 cut(s) 205, 595, 883, 979
MboII GAAGA 4 cut(s) 365, 388, 794, 963
MfeI CAATTG 1 cut(s) 1060
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 3 cut(s) 703, 716, 1060
MlyI GAGTC 2 cut(s) 121, 859
MmeI TCCRAC 2 cut(s) 173, 387
MseI TTAA 4 cut(s) 252, 771, 776, 1016
MspA1I CMGCKG 2 cut(s) 766, 938
MspI CCGG 1 cut(s) 755
MspR9I CCNGG 1 cut(s) 446
MunI CAATTG 1 cut(s) 1060
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 6 cut(s) 278, 301, 446, 500, 634, 874
NcoI CCATGG 1 cut(s) 195
NdeII GATC 4 cut(s) 205, 595, 883, 979
NlaIII CATG 7 cut(s) 199, 239, 299, 340, 483, 832, 935
NlaIV GGNNCC 4 cut(s) 95, 487, 730, 1008
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 3 cut(s) 173, 542, 685
NspI RCATGY 1 cut(s) 935
NspV TTCGAA 1 cut(s) 78
PaeI GCATGC 1 cut(s) 935
PceI AGGCCT 1 cut(s) 449
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 3 cut(s) 80, 573, 799
PflMI CCANNNNNTGG 3 cut(s) 19, 52, 451
PkrI GCNGC 3 cut(s) 313, 369, 937
PleI GAGTC 2 cut(s) 121, 858
PpsI GAGTC 2 cut(s) 121, 858
Psp6I CCWGG 1 cut(s) 444
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 4 cut(s) 95, 487, 730, 1008
PspPI GGNCC 3 cut(s) 242, 302, 1007
PstI CTGCAG 2 cut(s) 136, 1028
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 1 cut(s) 510
RsaNI GTAC 1 cut(s) 509
SalI GTCGAC 1 cut(s) 58
SaqAI TTAA 4 cut(s) 252, 771, 776, 1016
SatI GCNGC 3 cut(s) 312, 368, 936
Sau3AI GATC 4 cut(s) 205, 595, 883, 979
Sau96I GGNCC 3 cut(s) 242, 302, 1007
ScaI AGTACT 1 cut(s) 510
SchI GAGTC 2 cut(s) 121, 859
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 2 cut(s) 346, 379
SfcI CTRYAG 2 cut(s) 132, 1024
SfuI TTCGAA 1 cut(s) 78
SphI GCATGC 1 cut(s) 935
Sse9I AATT 3 cut(s) 703, 716, 1060
SseBI AGGCCT 1 cut(s) 449
SsiI CCGC 5 cut(s) 300, 368, 628, 766, 887
SspMI CTAG 2 cut(s) 506, 834
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 4 cut(s) 195, 450, 697, 1010
TaaI ACNGT 5 cut(s) 376, 467, 548, 894, 952
TaiI ACGT 1 cut(s) 625
TaqI TCGA 2 cut(s) 59, 78
TaqII GACCGA 1 cut(s) 77
TasI AATT 3 cut(s) 703, 716, 1060
TatI WGTACW 1 cut(s) 508
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 3 cut(s) 80, 573, 799
Tru1I TTAA 4 cut(s) 252, 771, 776, 1016
Tru9I TTAA 4 cut(s) 252, 771, 776, 1016
TscAI CASTG 5 cut(s) 472, 501, 852, 899, 955
TseFI GTSAC 3 cut(s) 173, 542, 685
TseI GCWGC 2 cut(s) 311, 935
Tsp45I GTSAC 3 cut(s) 173, 542, 685
TspDTI ATGAA 2 cut(s) 468, 474
TspRI CASTG 5 cut(s) 472, 501, 852, 899, 955
Van91I CCANNNNNTGG 3 cut(s) 19, 52, 451
XceI RCATGY 1 cut(s) 935
XmiI GTMKAC 1 cut(s) 59
XspI CTAG 2 cut(s) 506, 834
ZrmI AGTACT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.