Rorug04G0210300

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015600
Physical Location & Seq
Forward (+)
36640437 .. 36642657
2221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0210300.1

Sequence Viewer

Length: 1386 bp
ATGTCTCATGTTGCTACTATTGTTATACTGGGATTACTATTCCTACAAGCGGCAGAATGTGGAATACAGAATATTAAGAATGCAGTTACGTACTCCGCCATTAACTGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGAGCTGTTGGCGATGGAAAGACATTGAACACAAAGGCCTTTAAAGATGCAGTTGATAAACTAAGCCAGTATGCATCAGATGGTGGAGCACAGCTAATTGTGCCCCCCGGTAACTGGCTTACCGGGAGCTTCAACCTCACTAGCCACTTTACCCTTTTCCTCCACAAGGATGCTGTTATTCTTGGGTCTCAGGATGAGTCCGAGTGGCCTCTTCTTCCAGCACTGCCTTCATACGGGCAAGGGAGAGAGGCCCCTGGTGGAAGGTTTAGCAGTCTGATCTTTGGAACAAACCTTACCGACGTCGTAATTACCGGTAACAATGGCACCATTGATGGGCAAGGCGCTCCCTGGTGGGAGAAATTCAAGAAGGGTCTTTTGAATGTCACCCGACCATACTTGGTTGAGATTATGTACTCTAATAAGATCCAAATCTCCAATCTCACTTTTACGAATTCTCCATCCTGGCACATCCATCCCACTTACAGCAGTAACATTACTATACAAGGCCTCACTATCCTTGCACCAGTTAGCGTTCCTAACACAGATGGGATTAACCCCGATTCGTGTTCACAAACTCGAATTGAAGACTGTTACATAGTCTCCGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGTATGGAATCAAATTTGGAATGCCAACCGAGCATCTTGTTATCAGGAGGCTTACCTGTATTTCACCTAAAAGTGCTACTGTTGCCCTAGGCAGTGAAATGTCTGGTGGAATCAGGGATGTAAGAGTTGAAGATGTCACAGCCATTCACACCGAATCCAGCATTAGGATCAAAACAGCTGTAGGCAGAGGGGCTTATGTCAAAGACATTTATGTACGAAGATTGACGTTGAAGACTATGAAGTACGTTTTTTGGATGACAGGCGGCTATGGTTCACATCCAGGCGAGGGCTTTGATCCAAAGGCACTTCCTGCGATTCAAAACATCAACTATAGAGAGGTTGTTGCTGAGAATGTTACAATGCCAGCCCAGTTGGATGGAATTGCTAATGATCCTTTCAAGGGAATTTGCATCTCTAATGTGAGTATCTCACTCACTGAAAAGCCAAAGAAATTGTTGTGGAACTGTACTGATGTTGAGGGCATTACGAGCAATGTGACTCCAAAGGCTTGCGATTTGTTGCCCGAGCAGCAAAAACCAATCGATTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGCGTTAAGCTGACCACTTGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

461

Amino Acids

50.03

Weight (kDa)

6.32

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 137 - 419 5.6e-35 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 461
AciI CCGC 3 cut(s) 50, 96, 1041
AclWI GGATC 5 cut(s) 556, 783, 953, 1067, 1163
AcsI RAATTY 4 cut(s) 497, 589, 791, 1182
AcyI GRCGYC 1 cut(s) 438
AfaI GTAC 5 cut(s) 92, 551, 993, 1022, 1246
AfiI CCNNNNNNNGG 8 cut(s) 49, 252, 304, 371, 471, 942, 1064, 1178
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 9 cut(s) 166, 271, 502, 517, 722, 908, 1009, 1097, 1177
AjnI CCWGG 4 cut(s) 391, 485, 599, 1057
AluBI AGCT 5 cut(s) 143, 232, 267, 956, 1366
AluI AGCT 5 cut(s) 143, 232, 267, 956, 1366
Alw21I GWGCWC 2 cut(s) 124, 229
Alw26I GTCTC 3 cut(s) 9, 330, 742
AlwI GGATC 5 cut(s) 556, 783, 953, 1067, 1163
Ama87I CYCGRG 1 cut(s) 1301
AoxI GGCC 4 cut(s) 174, 344, 387, 643
ApeKI GCWGC 1 cut(s) 1306
ApoI RAATTY 4 cut(s) 497, 589, 791, 1182
AsiGI ACCGGT 1 cut(s) 449
AspA2I CCTAGG 1 cut(s) 865
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 388
AsuC2I CCSGG 3 cut(s) 246, 262, 742
AsuHPI GGTGA 2 cut(s) 514, 834
AvaI CYCGRG 1 cut(s) 1301
AvrII CCTAGG 1 cut(s) 865
AxyI CCTNAGG 1 cut(s) 1335
BaeGI GKGCMC 1 cut(s) 243
BanI GGYRCC 1 cut(s) 461
BbsI GAAGAC 2 cut(s) 729, 1016
Bbv12I GWGCWC 2 cut(s) 124, 229
BbvI GCAGC 1 cut(s) 1318
BccI CCATC 7 cut(s) 146, 212, 464, 604, 618, 677, 1148
BciT130I CCWGG 4 cut(s) 393, 487, 601, 1059
BcnI CCSGG 3 cut(s) 246, 262, 742
BcoDI GTCTC 3 cut(s) 9, 330, 742
BfaI CTAG 2 cut(s) 279, 866
BfmI CTRYAG 2 cut(s) 957, 1108
BfoI RGCGCY 1 cut(s) 483
BisI GCNGC 3 cut(s) 51, 1042, 1307
BlnI CCTAGG 1 cut(s) 865
BlsI GCNGC 3 cut(s) 52, 1043, 1308
Bme1390I CCNGG 7 cut(s) 246, 262, 393, 487, 601, 742, 1059
BmeT110I CYCGRG 1 cut(s) 1301
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 2 cut(s) 390, 463
BmrFI CCNGG 7 cut(s) 246, 262, 393, 487, 601, 742, 1059
BmrI ACTGGG 2 cut(s) 38, 1141
BmsI GCATC 5 cut(s) 175, 221, 298, 820, 1197
BmuI ACTGGG 2 cut(s) 38, 1141
BpiI GAAGAC 2 cut(s) 729, 1016
BpuMI CCSGG 3 cut(s) 246, 262, 742
Bsa29I ATCGAT 1 cut(s) 1320
BsaAI YACGTR 1 cut(s) 90
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 438
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 5 cut(s) 244, 391, 485, 741, 865
BsaWI WCCGGW 1 cut(s) 449
BsaXI ACNNNNNCTCC 4 cut(s) 577, 607, 722, 752
Bsc4I CCNNNNNNNGG 8 cut(s) 49, 252, 304, 371, 471, 942, 1064, 1178
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 5 cut(s) 33, 205, 257, 662, 1147
Bse21I CCTNAGG 1 cut(s) 1335
Bse3DI GCAATG 2 cut(s) 753, 1276
Bse8I GATNNNNATC 1 cut(s) 566
BseBI CCWGG 4 cut(s) 393, 487, 601, 1059
BseCI ATCGAT 1 cut(s) 1320
BseDI CCNNGG 5 cut(s) 244, 391, 485, 741, 865
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 8 cut(s) 49, 252, 304, 371, 471, 942, 1064, 1178
BseMI GCAATG 2 cut(s) 753, 1276
BseMII CTCAG 3 cut(s) 341, 1116, 1326
BseNI ACTGG 5 cut(s) 33, 205, 257, 662, 1147
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 1 cut(s) 1318
BseYI CCCAGC 1 cut(s) 771
BshFI GGCC 4 cut(s) 176, 346, 389, 645
BshNI GGYRCC 1 cut(s) 461
BshTI ACCGGT 1 cut(s) 449
BshVI ATCGAT 1 cut(s) 1320
BsiHKAI GWGCWC 2 cut(s) 124, 229
BsiHKCI CYCGRG 1 cut(s) 1301
BsiSI CCGG 5 cut(s) 109, 246, 261, 450, 741
BslI CCNNNNNNNGG 8 cut(s) 49, 252, 304, 371, 471, 942, 1064, 1178
BsmAI GTCTC 3 cut(s) 9, 330, 742
BsmI GAATGC 2 cut(s) 85, 804
BsnI GGCC 4 cut(s) 176, 346, 389, 645
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 1301
Bsp1286I GDGCHC 3 cut(s) 124, 229, 243
Bsp143I GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
BspACI CCGC 3 cut(s) 50, 96, 1041
BspANI GGCC 4 cut(s) 176, 346, 389, 645
BspCNI CTCAG 3 cut(s) 340, 1117, 1327
BspDI ATCGAT 1 cut(s) 1320
BspLI GGNNCC 2 cut(s) 390, 463
BspPI GGATC 5 cut(s) 556, 783, 953, 1067, 1163
BspT107I GGYRCC 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 330
BsrDI GCAATG 2 cut(s) 753, 1276
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 5 cut(s) 33, 205, 257, 662, 1147
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 5 cut(s) 244, 391, 485, 741, 865
BssMI GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
BssNI GRCGYC 1 cut(s) 438
BssT1I CCWWGG 1 cut(s) 865
Bst2UI CCWGG 4 cut(s) 393, 487, 601, 1059
Bst4CI ACNGT 4 cut(s) 119, 728, 859, 1244
Bst6I CTCTTC 2 cut(s) 354, 758
BstACI GRCGYC 1 cut(s) 438
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstBAI YACGTR 1 cut(s) 90
BstC8I GCNNGC 2 cut(s) 1143, 1288
BstDEI CTNAG 4 cut(s) 200, 327, 1125, 1335
BstENI CCTNNNNNAGG 1 cut(s) 302
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 6 cut(s) 417, 564, 778, 948, 1075, 1171
BstMAI GTCTC 3 cut(s) 9, 330, 742
BstMBI GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
BstMWI GCNNNNNNNGC 6 cut(s) 238, 808, 860, 1088, 1266, 1306
BstNI CCWGG 4 cut(s) 393, 487, 601, 1059
BstSCI CCNGG 7 cut(s) 244, 260, 391, 485, 599, 740, 1057
BstSFI CTRYAG 2 cut(s) 957, 1108
BstSLI GKGCMC 1 cut(s) 243
BstSNI TACGTA 1 cut(s) 90
BstV1I GCAGC 1 cut(s) 1318
BstV2I GAAGAC 2 cut(s) 729, 1016
BstX2I RGATCY 1 cut(s) 561
BstXI CCANNNNNNTGG 1 cut(s) 1154
BstYI RGATCY 1 cut(s) 561
Bsu15I ATCGAT 1 cut(s) 1320
Bsu36I CCTNAGG 1 cut(s) 1335
BsuRI GGCC 4 cut(s) 176, 346, 389, 645
BsuTUI ATCGAT 1 cut(s) 1320
BtgZI GCGATG 1 cut(s) 165
BtsI GCAGTG 3 cut(s) 359, 765, 877
BtsIMutI CAGTG 5 cut(s) 124, 359, 765, 877, 1212
Cac8I GCNNGC 2 cut(s) 1143, 1288
CfoI GCGC 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 388
ClaI ATCGAT 1 cut(s) 1320
Csp6I GTAC 5 cut(s) 91, 550, 992, 1021, 1245
CspAI ACCGGT 1 cut(s) 449
CviAII CATG 1 cut(s) 8
CviQI GTAC 5 cut(s) 91, 550, 992, 1021, 1245
DdeI CTNAG 4 cut(s) 200, 327, 1125, 1335
DpnI GATC 6 cut(s) 416, 563, 777, 947, 1074, 1170
DpnII GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
DraI TTTAAA 1 cut(s) 181
Eam1104I CTCTTC 2 cut(s) 354, 758
EarI CTCTTC 2 cut(s) 354, 758
EciI GGCGGA 1 cut(s) 85
Eco105I TACGTA 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 865
Eco147I AGGCCT 2 cut(s) 176, 645
Eco31I GGTCTC 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 1335
Eco88I CYCGRG 1 cut(s) 1301
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 589
EcoRII CCWGG 4 cut(s) 391, 485, 599, 1057
EcoT14I CCWWGG 1 cut(s) 865
EcoT22I ATGCAT 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 865
FaeI CATG 1 cut(s) 11
FatI CATG 1 cut(s) 7
Fnu4HI GCNGC 3 cut(s) 51, 1042, 1307
Fsp4HI GCNGC 3 cut(s) 51, 1042, 1307
FspBI CTAG 2 cut(s) 279, 866
GlaI GCGC 1 cut(s) 481
GluI GCNGC 3 cut(s) 51, 1042, 1307
GsaI CCCAGC 1 cut(s) 775
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 4 cut(s) 176, 346, 389, 645
HapII CCGG 5 cut(s) 109, 246, 261, 450, 741
HhaI GCGC 1 cut(s) 482
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 1 cut(s) 11
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HinfI GANTC 7 cut(s) 335, 698, 786, 888, 932, 1093, 1276
HpaII CCGG 5 cut(s) 109, 246, 261, 450, 741
HphI GGTGA 2 cut(s) 514, 834
Hpy166II GTNNAC 2 cut(s) 707, 1052
Hpy188I TCNGA 4 cut(s) 140, 217, 340, 414
Hpy188III TCNNGA 3 cut(s) 329, 502, 823
Hpy8I GTNNAC 2 cut(s) 707, 1052
Hpy99I CGWCG 2 cut(s) 440, 443
HpyAV CCTTC 3 cut(s) 375, 393, 499
HpyCH4III ACNGT 4 cut(s) 119, 728, 859, 1244
HpyCH4IV ACGT 4 cut(s) 89, 438, 1004, 1023
HpyCH4V TGCA 7 cut(s) 83, 188, 212, 659, 753, 758, 1188
HpyF10VI GCNNNNNNNGC 6 cut(s) 238, 808, 860, 1088, 1266, 1306
HpyF3I CTNAG 4 cut(s) 200, 327, 1125, 1335
HpySE526I ACGT 4 cut(s) 89, 438, 1004, 1023
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 1 cut(s) 11
HspAI GCGC 1 cut(s) 480
Kzo9I GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
LmnI GCTCC 4 cut(s) 140, 224, 264, 487
Lsp1109I GCAGC 1 cut(s) 1318
LweI GCATC 5 cut(s) 175, 221, 298, 820, 1197
MaeI CTAG 2 cut(s) 279, 866
MaeII ACGT 4 cut(s) 89, 438, 1004, 1023
MaeIII GTNAC 9 cut(s) 85, 248, 452, 520, 626, 728, 913, 1132, 1273
MalI GATC 6 cut(s) 416, 563, 777, 947, 1074, 1170
MboI GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
MboII GAAGA 7 cut(s) 341, 344, 734, 775, 920, 1008, 1021
MflI RGATCY 1 cut(s) 561
MhlI GDGCHC 3 cut(s) 124, 229, 243
MluCI AATT 9 cut(s) 234, 444, 497, 589, 717, 791, 1158, 1182, 1229
MlyI GAGTC 2 cut(s) 344, 1270
MmeI TCCRAC 1 cut(s) 1131
Mph1103I ATGCAT 1 cut(s) 214
MseI TTAA 6 cut(s) 75, 102, 180, 690, 1362, 1384
MslI CAYNNNNRTG 2 cut(s) 306, 758
MspA1I CMGCKG 1 cut(s) 956
MspI CCGG 5 cut(s) 109, 246, 261, 450, 741
MspR9I CCNGG 7 cut(s) 246, 262, 393, 487, 601, 742, 1059
Mva1269I GAATGC 2 cut(s) 85, 804
MvaI CCWGG 4 cut(s) 393, 487, 601, 1059
MwoI GCNNNNNNNGC 6 cut(s) 238, 808, 860, 1088, 1266, 1306
NciI CCSGG 3 cut(s) 246, 262, 742
NdeII GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
NlaIII CATG 1 cut(s) 11
NlaIV GGNNCC 2 cut(s) 390, 463
NmuCI GTSAC 3 cut(s) 520, 913, 1273
NsiI ATGCAT 1 cut(s) 214
PceI AGGCCT 2 cut(s) 176, 645
PctI GAATGC 2 cut(s) 85, 804
PfeI GAWTC 5 cut(s) 698, 786, 888, 932, 1093
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 3 cut(s) 52, 1043, 1308
PleI GAGTC 2 cut(s) 343, 1270
PpsI GAGTC 2 cut(s) 343, 1270
Ppu21I YACGTR 1 cut(s) 90
Psp6I CCWGG 4 cut(s) 391, 485, 599, 1057
PspFI CCCAGC 1 cut(s) 771
PspGI CCWGG 4 cut(s) 391, 485, 599, 1057
PspN4I GGNNCC 2 cut(s) 390, 463
PspPI GGNCC 1 cut(s) 388
PsrI GAACNNNNNNTAC 2 cut(s) 415, 447
PsuI RGATCY 1 cut(s) 561
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 5 cut(s) 92, 551, 993, 1022, 1246
RsaNI GTAC 5 cut(s) 91, 550, 992, 1021, 1245
RseI CAYNNNNRTG 2 cut(s) 306, 758
SaqAI TTAA 6 cut(s) 75, 102, 180, 690, 1362, 1384
SatI GCNGC 3 cut(s) 51, 1042, 1307
Sau3AI GATC 6 cut(s) 414, 561, 775, 945, 1072, 1168
Sau96I GGNCC 1 cut(s) 388
SchI GAGTC 2 cut(s) 344, 1270
ScrFI CCNGG 7 cut(s) 246, 262, 393, 487, 601, 742, 1059
SduI GDGCHC 3 cut(s) 124, 229, 243
SfaNI GCATC 5 cut(s) 175, 221, 298, 820, 1197
SfcI CTRYAG 2 cut(s) 957, 1108
SmiMI CAYNNNNRTG 2 cut(s) 306, 758
SnaBI TACGTA 1 cut(s) 90
Sse9I AATT 9 cut(s) 234, 444, 497, 589, 717, 791, 1158, 1182, 1229
SseBI AGGCCT 2 cut(s) 176, 645
SsiI CCGC 3 cut(s) 50, 96, 1041
SspI AATATT 1 cut(s) 73
SspMI CTAG 2 cut(s) 279, 866
StuI AGGCCT 2 cut(s) 176, 645
StyD4I CCNGG 7 cut(s) 244, 260, 391, 485, 599, 740, 1057
StyI CCWWGG 1 cut(s) 865
TaaI ACNGT 4 cut(s) 119, 728, 859, 1244
TaiI ACGT 4 cut(s) 92, 441, 1007, 1026
TaqI TCGA 2 cut(s) 715, 1320
TasI AATT 9 cut(s) 234, 444, 497, 589, 717, 791, 1158, 1182, 1229
TatI WGTACW 2 cut(s) 549, 1244
TauI GCSGC 2 cut(s) 53, 1044
TfiI GAWTC 5 cut(s) 698, 786, 888, 932, 1093
Tru1I TTAA 6 cut(s) 75, 102, 180, 690, 1362, 1384
Tru9I TTAA 6 cut(s) 75, 102, 180, 690, 1362, 1384
TscAI CASTG 5 cut(s) 124, 366, 765, 877, 1219
TseFI GTSAC 3 cut(s) 520, 913, 1273
TseI GCWGC 1 cut(s) 1306
Tsp45I GTSAC 3 cut(s) 520, 913, 1273
TspDTI ATGAA 2 cut(s) 357, 1031
TspRI CASTG 5 cut(s) 124, 366, 765, 877, 1219
XagI CCTNNNNNAGG 1 cut(s) 302
XapI RAATTY 4 cut(s) 497, 589, 791, 1182
XmaJI CCTAGG 1 cut(s) 865
XspI CTAG 2 cut(s) 279, 866
ZraI GACGTC 1 cut(s) 439
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.