RLG00000007301

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015600
Physical Location & Seq
Forward (+)
15552515 .. 15554984
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007301

Sequence Viewer

Length: 1026 bp
ATGAAGGACACCTGTACTAACCTAATTCTGTGCATTTTTTATCAGGTTGTGAGGTGCTCAGAGACCATTGCTAATGCTGTAATAGAGACTCCTGCAGTCAAGTTTGTTCCAACCATTCACTCAGGGAGCCATAGTGACATCGGAGTAAGGCGTTCCATGGATGACGATCACATTCGGATCGATGACTTATCTGGCCATGTTGGGCCTCTCTTTAAGAGTTCATTGCCAAGTGCATTTTATGCAGTTTTTGATGGACATGGCGGGCCTGAAGCAGCTACTTATATCAAGAGGAATGCCATGAGACTATTTTTTGAAGATGCTGATTTGCCGCATGCAGTAGATTTTGATGATGCTTTCTTCAAAGAGTTGGAGAGCTCACATAAGAAAGCATTTTTACTGGCAGACCAGGCCTTGGCCGATGAACACAGTGTGGATGGTTCATGTGGAACCACAGCATTGACTGCCCTAGTACTTGGAAGGCAGTTGCTCGTAGCAAATGCTGGTGACTGTCGGGCAGTTCTTTGCAGGAAAGGAATCGCATTTGATATGTCCCAAGATCATAAGCCTACTTATTTGCCAGAACGTAGGCGGGTGGAGCAGTTGGGTGGTTACTTTGTTGATGGGTATCTGAATGGTGATCTCTCAGTCACCCGAACCCTTGGAAATTGGGATTTCAAATTACCACTTGGCTCCTCATCACCTCTCATTGCTGAACCGGATGTTCAGCGGGTTAAGTTGACAGAAGATGATGAGTTTCTGATTCTTAGTTGTGATGGGATATGGGATGTCATGTCTAGCCAATATGCAGTGAGTCTTGTTCGGCGTGAGCTGAGGAAGCACAATGATCCACAACAGTGTGCCAGAGAACTCGTGAAAGAAGCACTACGCAAGCATGCATCTGACAATCTCACTGTTATTGTTGTATGCCTCTCTTCTCCTGATCGTGCTGTTGAGCCTCGCTCTGAGGGGCCAAGGTTAAGGAACTGCAGTTTTTCAAGGTTGAGAAGCTTGCTGGAAGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

342

Amino Acids

37.83

Weight (kDa)

5.68

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 42 - 302 1.7e-64 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 412
AciI CCGC 4 cut(s) 261, 329, 589, 727
AclWI GGATC 2 cut(s) 185, 839
AcoI YGGCCR 2 cut(s) 193, 414
AcuI CTGAAG 1 cut(s) 288
AdeI CACNNNGTG 1 cut(s) 430
AfaI GTAC 2 cut(s) 16, 471
AfiI CCNNNNNNNGG 1 cut(s) 412
AgsI TTSAA 4 cut(s) 314, 361, 676, 996
AjnI CCWGG 1 cut(s) 405
AleI CACNNNNGTG 1 cut(s) 853
AluBI AGCT 4 cut(s) 275, 375, 829, 1008
AluI AGCT 4 cut(s) 275, 375, 829, 1008
Alw21I GWGCWC 2 cut(s) 59, 377
Alw26I GTCTC 3 cut(s) 56, 80, 295
AlwI GGATC 2 cut(s) 185, 839
AoxI GGCC 6 cut(s) 193, 203, 263, 408, 414, 968
ApeKI GCWGC 1 cut(s) 272
AspS9I GGNCC 3 cut(s) 203, 263, 968
AsuHPI GGTGA 4 cut(s) 515, 640, 647, 690
BalI TGGCCA 1 cut(s) 195
BanII GRGCYC 1 cut(s) 377
BauI CACGAG 1 cut(s) 869
Bbv12I GWGCWC 2 cut(s) 59, 377
BbvCI CCTCAGC 1 cut(s) 830
BbvI GCAGC 1 cut(s) 284
BccI CCATC 4 cut(s) 245, 428, 614, 767
BciT130I CCWGG 1 cut(s) 407
BcoDI GTCTC 3 cut(s) 56, 80, 295
BfaI CTAG 2 cut(s) 467, 795
BfmI CTRYAG 2 cut(s) 93, 985
BisI GCNGC 2 cut(s) 273, 329
BlsI GCNGC 2 cut(s) 274, 330
BmcAI AGTACT 1 cut(s) 471
Bme1390I CCNGG 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 203, 263, 968
BmiI GGNNCC 4 cut(s) 128, 448, 691, 969
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 3 cut(s) 307, 340, 905
BplI GAGNNNNNCTC 2 cut(s) 944, 976
Bpu10I CCTNAGC 1 cut(s) 830
Bsa29I ATCGAT 1 cut(s) 180
BsaBI GATNNNNATC 2 cut(s) 165, 624
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 4 cut(s) 156, 411, 658, 971
BsaWI WCCGGW 1 cut(s) 715
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse1I ACTGG 1 cut(s) 402
Bse3DI GCAATG 3 cut(s) 66, 221, 705
Bse8I GATNNNNATC 2 cut(s) 165, 624
BseBI CCWGG 1 cut(s) 407
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 4 cut(s) 156, 411, 658, 971
BseGI GGATG 4 cut(s) 166, 439, 724, 790
BseJI GATNNNNATC 2 cut(s) 165, 624
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 3 cut(s) 66, 221, 705
BseMII CTCAG 5 cut(s) 72, 135, 657, 821, 954
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 682
BseXI GCAGC 1 cut(s) 284
BshFI GGCC 6 cut(s) 195, 205, 265, 410, 416, 970
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 59, 377
BsiSI CCGG 1 cut(s) 716
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 3 cut(s) 56, 80, 295
BsmFI GGGAC 1 cut(s) 535
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 6 cut(s) 195, 205, 265, 410, 416, 970
Bso31I GGTCTC 1 cut(s) 56
Bsp1286I GDGCHC 2 cut(s) 59, 377
Bsp143I GATC 6 cut(s) 166, 177, 556, 637, 844, 940
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 4 cut(s) 261, 329, 589, 727
BspANI GGCC 6 cut(s) 195, 205, 265, 410, 416, 970
BspCNI CTCAG 5 cut(s) 71, 134, 656, 822, 955
BspDI ATCGAT 1 cut(s) 180
BspLI GGNNCC 4 cut(s) 128, 448, 691, 969
BspMAI CTGCAG 2 cut(s) 97, 989
BspPI GGATC 2 cut(s) 185, 839
BspTNI GGTCTC 1 cut(s) 56
BsrDI GCAATG 3 cut(s) 66, 221, 705
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 4 cut(s) 156, 411, 658, 971
BssMI GATC 6 cut(s) 166, 177, 556, 637, 844, 940
BssSI CACGAG 1 cut(s) 869
BssT1I CCWWGG 4 cut(s) 156, 411, 658, 971
Bst2BI CACGAG 1 cut(s) 869
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 4 cut(s) 428, 509, 855, 913
Bst6I CTCTTC 1 cut(s) 937
BstAPI GCANNNNNTGC 2 cut(s) 239, 461
BstC8I GCNNGC 5 cut(s) 263, 333, 890, 894, 1010
BstDEI CTNAG 6 cut(s) 58, 121, 643, 764, 830, 963
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 4 cut(s) 166, 439, 724, 790
BstKTI GATC 6 cut(s) 169, 180, 559, 640, 847, 943
BstMAI GTCTC 3 cut(s) 56, 80, 295
BstMBI GATC 6 cut(s) 166, 177, 556, 637, 844, 940
BstMWI GCNNNNNNNGC 5 cut(s) 239, 407, 461, 595, 835
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 2 cut(s) 335, 896
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 2 cut(s) 93, 985
BstV1I GCAGC 1 cut(s) 284
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 6 cut(s) 195, 205, 265, 410, 416, 970
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 4 cut(s) 166, 439, 724, 790
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 4 cut(s) 433, 813, 860, 909
Cac8I GCNNGC 5 cut(s) 263, 333, 890, 894, 1010
Cfr13I GGNCC 3 cut(s) 203, 263, 968
ClaI ATCGAT 1 cut(s) 180
Csp6I GTAC 2 cut(s) 15, 470
CviAII CATG 8 cut(s) 157, 197, 257, 298, 332, 441, 790, 893
CviQI GTAC 2 cut(s) 15, 470
DdeI CTNAG 6 cut(s) 58, 121, 643, 764, 830, 963
DpnI GATC 6 cut(s) 168, 179, 558, 639, 846, 942
DpnII GATC 6 cut(s) 166, 177, 556, 637, 844, 940
DraIII CACNNNGTG 1 cut(s) 430
EaeI YGGCCR 2 cut(s) 193, 414
Eam1104I CTCTTC 1 cut(s) 937
EarI CTCTTC 1 cut(s) 937
Ecl136II GAGCTC 1 cut(s) 375
Eco130I CCWWGG 4 cut(s) 156, 411, 658, 971
Eco147I AGGCCT 1 cut(s) 410
Eco24I GRGCYC 1 cut(s) 377
Eco31I GGTCTC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 375
Eco57I CTGAAG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 405
EcoT14I CCWWGG 4 cut(s) 156, 411, 658, 971
EcoT22I ATGCAT 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 377
ErhI CCWWGG 4 cut(s) 156, 411, 658, 971
FaeI CATG 8 cut(s) 160, 200, 260, 301, 335, 444, 793, 896
FaqI GGGAC 1 cut(s) 535
FatI CATG 8 cut(s) 156, 196, 256, 297, 331, 440, 789, 892
FauI CCCGC 3 cut(s) 254, 582, 720
Fnu4HI GCNGC 2 cut(s) 273, 329
FokI GGATG 4 cut(s) 173, 446, 731, 797
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 2 cut(s) 273, 329
FspBI CTAG 2 cut(s) 467, 795
GluI GCNGC 2 cut(s) 273, 329
HaeIII GGCC 6 cut(s) 195, 205, 265, 410, 416, 970
HapII CCGG 1 cut(s) 716
Hin1II CATG 8 cut(s) 160, 200, 260, 301, 335, 444, 793, 896
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 1 cut(s) 1006
HinfI GANTC 4 cut(s) 88, 534, 760, 811
HpaII CCGG 1 cut(s) 716
HphI GGTGA 4 cut(s) 515, 640, 647, 690
Hpy166II GTNNAC 1 cut(s) 738
Hpy188I TCNGA 7 cut(s) 61, 143, 177, 630, 759, 901, 964
Hpy188III TCNNGA 3 cut(s) 286, 871, 938
Hpy8I GTNNAC 1 cut(s) 738
HpyAV CCTTC 2 cut(s) 471, 1010
HpyCH4III ACNGT 4 cut(s) 428, 509, 855, 913
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 9 cut(s) 33, 95, 233, 242, 335, 525, 806, 896, 987
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 407, 461, 595, 835
HpyF3I CTNAG 6 cut(s) 58, 121, 643, 764, 830, 963
HpySE526I ACGT 1 cut(s) 583
Hsp92II CATG 8 cut(s) 160, 200, 260, 301, 335, 444, 793, 896
Kzo9I GATC 6 cut(s) 166, 177, 556, 637, 844, 940
LmnI GCTCC 3 cut(s) 126, 595, 695
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 3 cut(s) 307, 340, 905
MaeI CTAG 2 cut(s) 467, 795
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 4 cut(s) 134, 503, 608, 646
MalI GATC 6 cut(s) 168, 179, 558, 639, 846, 942
MboI GATC 6 cut(s) 166, 177, 556, 637, 844, 940
MboII GAAGA 4 cut(s) 326, 349, 755, 924
MfeI CAATTG 1 cut(s) 1021
MhlI GDGCHC 2 cut(s) 59, 377
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 4 cut(s) 24, 664, 677, 1021
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 2 cut(s) 82, 820
MmeI TCCRAC 2 cut(s) 134, 348
MnlI CCTC 9 cut(s) 45, 216, 282, 703, 711, 825, 938, 958, 966
Mox20I TGGCCA 1 cut(s) 195
Mph1103I ATGCAT 1 cut(s) 898
MscI TGGCCA 1 cut(s) 195
MseI TTAA 3 cut(s) 213, 732, 977
MslI CAYNNNNRTG 1 cut(s) 853
Msp20I TGGCCA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 727
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 1 cut(s) 407
MunI CAATTG 1 cut(s) 1021
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 5 cut(s) 239, 407, 461, 595, 835
NcoI CCATGG 1 cut(s) 156
NdeII GATC 6 cut(s) 166, 177, 556, 637, 844, 940
NlaIII CATG 8 cut(s) 160, 200, 260, 301, 335, 444, 793, 896
NlaIV GGNNCC 4 cut(s) 128, 448, 691, 969
NmuCI GTSAC 3 cut(s) 134, 503, 646
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 2 cut(s) 335, 896
OliI CACNNNNGTG 1 cut(s) 853
PaeI GCATGC 2 cut(s) 335, 896
PceI AGGCCT 1 cut(s) 410
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 534, 760
PflMI CCANNNNNTGG 1 cut(s) 412
PkrI GCNGC 2 cut(s) 274, 330
PleI GAGTC 2 cut(s) 82, 819
PpsI GAGTC 2 cut(s) 82, 819
Psp124BI GAGCTC 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 4 cut(s) 128, 448, 691, 969
PspPI GGNCC 3 cut(s) 203, 263, 968
PstI CTGCAG 2 cut(s) 97, 989
RsaI GTAC 2 cut(s) 16, 471
RsaNI GTAC 2 cut(s) 15, 470
RseI CAYNNNNRTG 1 cut(s) 853
SacI GAGCTC 1 cut(s) 377
SaqAI TTAA 3 cut(s) 213, 732, 977
SatI GCNGC 2 cut(s) 273, 329
Sau3AI GATC 6 cut(s) 166, 177, 556, 637, 844, 940
Sau96I GGNCC 3 cut(s) 203, 263, 968
ScaI AGTACT 1 cut(s) 471
SchI GAGTC 2 cut(s) 82, 820
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 2 cut(s) 59, 377
SfaNI GCATC 3 cut(s) 307, 340, 905
SfcI CTRYAG 2 cut(s) 93, 985
SmiMI CAYNNNNRTG 1 cut(s) 853
SphI GCATGC 2 cut(s) 335, 896
Sse9I AATT 4 cut(s) 24, 664, 677, 1021
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 4 cut(s) 261, 329, 589, 727
SspMI CTAG 2 cut(s) 467, 795
SstI GAGCTC 1 cut(s) 377
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 4 cut(s) 156, 411, 658, 971
TaaI ACNGT 4 cut(s) 428, 509, 855, 913
TaiI ACGT 1 cut(s) 586
TaqI TCGA 1 cut(s) 180
TasI AATT 4 cut(s) 24, 664, 677, 1021
TatI WGTACW 2 cut(s) 14, 469
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 2 cut(s) 534, 760
Tru1I TTAA 3 cut(s) 213, 732, 977
Tru9I TTAA 3 cut(s) 213, 732, 977
TscAI CASTG 4 cut(s) 433, 813, 860, 916
TseFI GTSAC 3 cut(s) 134, 503, 646
TseI GCWGC 1 cut(s) 272
Tsp45I GTSAC 3 cut(s) 134, 503, 646
TspDTI ATGAA 4 cut(s) 17, 210, 429, 435
TspRI CASTG 4 cut(s) 433, 813, 860, 916
Van91I CCANNNNNTGG 1 cut(s) 412
XceI RCATGY 2 cut(s) 335, 896
XspI CTAG 2 cut(s) 467, 795
ZrmI AGTACT 1 cut(s) 471
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.