Rmu_sc0020213.1_g000001

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020213.1
OG0015600
Physical Location & Seq
Forward (+)
476 .. 1868
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020213.1_g000001.1.cds

Sequence Viewer

Length: 1053 bp
atgggtgggtcaggaaattgggagtttagtttgtttctggttgtgaggtgcccagagaccattgctaatgctgttgtgaggtgcccagagaccattgctaatgctgtaatagagactcctgcagtcaagtttgttccaaccattcactcagggagccatagtgacatcggaggaaggcattccatggaagacgatcacattcggatcgatgacctatctgcccatgttgggcctctctttaagagttccttgccaagtgcattttatgcagtttttgatgggcatggcgggcctgaagcagctacttatatcaagaggaatgccatgagactattttttgaagatgctgacttgccgcatacagtagattttgatgatgctttcttcaaagagttggagaactcacataagaaagcatttttactggcagaccaggccttggccgatgaacacagtgtgggtggtttatgtggaaccacagcactgactgccctagtacttggaaggcagttgctcgtagcaaatgctggtgactgtcgggcagttctttgcaggaagggaatcgcatttgatatgtcccaagatcataagcatacttacttgccagaacgtaggcgggtggagcagttgggtggttactttgatggtgagtatctgaatagttatctctcagtcacccgaacccttggaaattgggatttcaaattaccatttggctcctcatcacctctcattgctgaaccggaggttcagcgggttaagttaacagaagatgatgagtttctgattattagttgtgatgggatatgggatgtcatgtctagccaatatgcagtgagtcttgttcggcgtgagctgaggaagcacagtgatccgcaacagtgtgccagagaactcgtgaaagaagcactacgcatgcatgcagctgacaatctcactgttattgttgtatgcctctcttctcctgatcgcgctgttgagcctcgctctgaggggccaaggttaaggaactgcagtttttcaaggttgagaagcttgctggaaggcaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

350

Amino Acids

38.78

Weight (kDa)

5.63

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 48, 81
AccB7I CCANNNNNTGG 1 cut(s) 439
AccII CGCG 1 cut(s) 972
AciI CCGC 5 cut(s) 288, 356, 616, 754, 875
AclWI GGATC 2 cut(s) 212, 866
AcoI YGGCCR 1 cut(s) 441
AcuI CTGAAG 1 cut(s) 315
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 2 cut(s) 228, 439
AgsI TTSAA 4 cut(s) 341, 388, 703, 1023
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 4 cut(s) 302, 856, 926, 1035
AluI AGCT 4 cut(s) 302, 856, 926, 1035
Alw26I GTCTC 4 cut(s) 50, 83, 107, 322
AlwI GGATC 2 cut(s) 212, 866
AoxI GGCC 5 cut(s) 230, 290, 435, 441, 995
ApeKI GCWGC 2 cut(s) 299, 923
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 1 cut(s) 974
AspS9I GGNCC 3 cut(s) 230, 290, 995
AsuHPI GGTGA 4 cut(s) 542, 659, 667, 717
BaeGI GKGCMC 2 cut(s) 53, 86
BanI GGYRCC 2 cut(s) 48, 81
BauI CACGAG 1 cut(s) 896
BbsI GAAGAC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 2 cut(s) 311, 935
BccI CCATC 3 cut(s) 272, 638, 794
BciT130I CCWGG 1 cut(s) 434
BcoDI GTCTC 4 cut(s) 50, 83, 107, 322
BfaI CTAG 2 cut(s) 494, 822
BfmI CTRYAG 2 cut(s) 120, 1012
BisI GCNGC 3 cut(s) 300, 356, 924
BlsI GCNGC 3 cut(s) 301, 357, 925
BmcAI AGTACT 1 cut(s) 498
Bme1390I CCNGG 1 cut(s) 434
BmgT120I GGNCC 3 cut(s) 230, 290, 995
BmiI GGNNCC 6 cut(s) 50, 83, 155, 475, 718, 996
BmrFI CCNGG 1 cut(s) 434
BmsI GCATC 2 cut(s) 334, 367
BpiI GAAGAC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
Bpu10I CCTNAGC 1 cut(s) 857
Bsa29I ATCGAT 1 cut(s) 207
BsaI GGTCTC 2 cut(s) 50, 83
BsaJI CCNNGG 4 cut(s) 183, 438, 685, 998
BsaWI WCCGGW 1 cut(s) 742
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 439
Bse1I ACTGG 1 cut(s) 429
Bse3DI GCAATG 3 cut(s) 60, 93, 732
BseBI CCWGG 1 cut(s) 434
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 4 cut(s) 183, 438, 685, 998
BseGI GGATG 1 cut(s) 817
BseLI CCNNNNNNNGG 2 cut(s) 228, 439
BseMI GCAATG 3 cut(s) 60, 93, 732
BseMII CTCAG 4 cut(s) 162, 684, 848, 981
BseNI ACTGG 1 cut(s) 429
BseRI GAGGAG 1 cut(s) 709
BseSI GKGCMC 2 cut(s) 53, 86
BseXI GCAGC 2 cut(s) 311, 935
Bsh1236I CGCG 1 cut(s) 972
BshFI GGCC 5 cut(s) 232, 292, 437, 443, 997
BshNI GGYRCC 2 cut(s) 48, 81
BshVI ATCGAT 1 cut(s) 207
BsiSI CCGG 1 cut(s) 743
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 2 cut(s) 228, 439
BsmAI GTCTC 4 cut(s) 50, 83, 107, 322
BsmFI GGGAC 1 cut(s) 562
BsmI GAATGC 2 cut(s) 178, 325
BsnI GGCC 5 cut(s) 232, 292, 437, 443, 997
Bso31I GGTCTC 2 cut(s) 50, 83
Bsp1286I GDGCHC 2 cut(s) 53, 86
Bsp143I GATC 5 cut(s) 193, 204, 583, 871, 967
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 5 cut(s) 288, 356, 616, 754, 875
BspANI GGCC 5 cut(s) 232, 292, 437, 443, 997
BspCNI CTCAG 4 cut(s) 161, 683, 849, 982
BspDI ATCGAT 1 cut(s) 207
BspFNI CGCG 1 cut(s) 972
BspLI GGNNCC 6 cut(s) 50, 83, 155, 475, 718, 996
BspMAI CTGCAG 2 cut(s) 124, 1016
BspPI GGATC 2 cut(s) 212, 866
BspT107I GGYRCC 2 cut(s) 48, 81
BspTNI GGTCTC 2 cut(s) 50, 83
BsrDI GCAATG 3 cut(s) 60, 93, 732
BsrI ACTGG 1 cut(s) 429
BssECI CCNNGG 4 cut(s) 183, 438, 685, 998
BssMI GATC 5 cut(s) 193, 204, 583, 871, 967
BssSI CACGAG 1 cut(s) 896
BssT1I CCWWGG 4 cut(s) 183, 438, 685, 998
Bst2BI CACGAG 1 cut(s) 896
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 6 cut(s) 364, 455, 536, 869, 882, 940
Bst6I CTCTTC 1 cut(s) 964
BstAPI GCANNNNNTGC 2 cut(s) 266, 488
BstC8I GCNNGC 4 cut(s) 290, 917, 921, 1037
BstDEI CTNAG 4 cut(s) 148, 670, 857, 990
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 817
BstFNI CGCG 1 cut(s) 972
BstHHI GCGC 1 cut(s) 974
BstKTI GATC 5 cut(s) 196, 207, 586, 874, 970
BstMAI GTCTC 4 cut(s) 50, 83, 107, 322
BstMBI GATC 5 cut(s) 193, 204, 583, 871, 967
BstMWI GCNNNNNNNGC 6 cut(s) 266, 289, 434, 488, 622, 862
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 2 cut(s) 919, 923
BstSCI CCNGG 1 cut(s) 432
BstSFI CTRYAG 2 cut(s) 120, 1012
BstSLI GKGCMC 2 cut(s) 53, 86
BstUI CGCG 1 cut(s) 972
BstV1I GCAGC 2 cut(s) 311, 935
BstV2I GAAGAC 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 5 cut(s) 232, 292, 437, 443, 997
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 817
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 6 cut(s) 460, 482, 840, 874, 887, 936
Cac8I GCNNGC 4 cut(s) 290, 917, 921, 1037
CfoI GCGC 1 cut(s) 974
Cfr13I GGNCC 3 cut(s) 230, 290, 995
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 1 cut(s) 497
CviAII CATG 7 cut(s) 184, 224, 284, 325, 817, 916, 920
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 4 cut(s) 148, 670, 857, 990
DpnI GATC 5 cut(s) 195, 206, 585, 873, 969
DpnII GATC 5 cut(s) 193, 204, 583, 871, 967
DraIII CACNNNGTG 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 964
EarI CTCTTC 1 cut(s) 964
Eco130I CCWWGG 4 cut(s) 183, 438, 685, 998
Eco147I AGGCCT 1 cut(s) 437
Eco31I GGTCTC 2 cut(s) 50, 83
Eco57I CTGAAG 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 432
EcoT14I CCWWGG 4 cut(s) 183, 438, 685, 998
EcoT22I ATGCAT 1 cut(s) 921
ErhI CCWWGG 4 cut(s) 183, 438, 685, 998
FaeI CATG 7 cut(s) 187, 227, 287, 328, 820, 919, 923
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 562
FatI CATG 7 cut(s) 183, 223, 283, 324, 816, 915, 919
FauI CCCGC 3 cut(s) 281, 609, 747
Fnu4HI GCNGC 3 cut(s) 300, 356, 924
FokI GGATG 1 cut(s) 824
Fsp4HI GCNGC 3 cut(s) 300, 356, 924
FspBI CTAG 2 cut(s) 494, 822
GlaI GCGC 1 cut(s) 973
GluI GCNGC 3 cut(s) 300, 356, 924
HaeIII GGCC 5 cut(s) 232, 292, 437, 443, 997
HapII CCGG 1 cut(s) 743
HhaI GCGC 1 cut(s) 974
Hin1II CATG 7 cut(s) 187, 227, 287, 328, 820, 919, 923
Hin6I GCGC 1 cut(s) 972
HinP1I GCGC 1 cut(s) 972
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 1033
HinfI GANTC 3 cut(s) 115, 561, 838
HpaI GTTAAC 1 cut(s) 765
HpaII CCGG 1 cut(s) 743
HphI GGTGA 4 cut(s) 542, 659, 667, 717
Hpy166II GTNNAC 1 cut(s) 765
Hpy188I TCNGA 5 cut(s) 170, 204, 657, 786, 991
Hpy188III TCNNGA 4 cut(s) 12, 313, 898, 965
Hpy8I GTNNAC 1 cut(s) 765
HpyAV CCTTC 4 cut(s) 168, 498, 550, 1037
HpyCH4III ACNGT 6 cut(s) 364, 455, 536, 869, 882, 940
HpyCH4IV ACGT 1 cut(s) 610
HpyCH4V TGCA 8 cut(s) 122, 260, 269, 552, 833, 919, 923, 1014
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 289, 434, 488, 622, 862
HpyF3I CTNAG 4 cut(s) 148, 670, 857, 990
HpySE526I ACGT 1 cut(s) 610
Hsp92II CATG 7 cut(s) 187, 227, 287, 328, 820, 919, 923
HspAI GCGC 1 cut(s) 972
KspAI GTTAAC 1 cut(s) 765
Kzo9I GATC 5 cut(s) 193, 204, 583, 871, 967
LmnI GCTCC 3 cut(s) 153, 622, 722
Lsp1109I GCAGC 2 cut(s) 311, 935
LweI GCATC 2 cut(s) 334, 367
MaeI CTAG 2 cut(s) 494, 822
MaeII ACGT 1 cut(s) 610
MaeIII GTNAC 4 cut(s) 161, 530, 635, 673
MalI GATC 5 cut(s) 195, 206, 585, 873, 969
MboI GATC 5 cut(s) 193, 204, 583, 871, 967
MboII GAAGA 5 cut(s) 200, 353, 376, 782, 951
MfeI CAATTG 1 cut(s) 1048
MhlI GDGCHC 2 cut(s) 53, 86
MluCI AATT 4 cut(s) 16, 691, 704, 1048
MlyI GAGTC 2 cut(s) 109, 847
MmeI TCCRAC 2 cut(s) 161, 375
Mph1103I ATGCAT 1 cut(s) 921
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 4 cut(s) 240, 759, 764, 1004
MspA1I CMGCKG 2 cut(s) 754, 926
MspI CCGG 1 cut(s) 743
MspR9I CCNGG 1 cut(s) 434
MunI CAATTG 1 cut(s) 1048
Mva1269I GAATGC 2 cut(s) 178, 325
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 1 cut(s) 972
MwoI GCNNNNNNNGC 6 cut(s) 266, 289, 434, 488, 622, 862
NcoI CCATGG 1 cut(s) 183
NdeII GATC 5 cut(s) 193, 204, 583, 871, 967
NlaIII CATG 7 cut(s) 187, 227, 287, 328, 820, 919, 923
NlaIV GGNNCC 6 cut(s) 50, 83, 155, 475, 718, 996
NmuCI GTSAC 3 cut(s) 161, 530, 673
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 2 cut(s) 919, 923
PaeI GCATGC 2 cut(s) 919, 923
PceI AGGCCT 1 cut(s) 437
PctI GAATGC 2 cut(s) 178, 325
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 561
PflMI CCANNNNNTGG 1 cut(s) 439
PkrI GCNGC 3 cut(s) 301, 357, 925
PleI GAGTC 2 cut(s) 109, 846
PpsI GAGTC 2 cut(s) 109, 846
Psp6I CCWGG 1 cut(s) 432
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 6 cut(s) 50, 83, 155, 475, 718, 996
PspPI GGNCC 3 cut(s) 230, 290, 995
PstI CTGCAG 2 cut(s) 124, 1016
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
SaqAI TTAA 4 cut(s) 240, 759, 764, 1004
SatI GCNGC 3 cut(s) 300, 356, 924
Sau3AI GATC 5 cut(s) 193, 204, 583, 871, 967
Sau96I GGNCC 3 cut(s) 230, 290, 995
ScaI AGTACT 1 cut(s) 498
SchI GAGTC 2 cut(s) 109, 847
ScrFI CCNGG 1 cut(s) 434
SduI GDGCHC 2 cut(s) 53, 86
SfaNI GCATC 2 cut(s) 334, 367
SfcI CTRYAG 2 cut(s) 120, 1012
SphI GCATGC 2 cut(s) 919, 923
Sse9I AATT 4 cut(s) 16, 691, 704, 1048
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 5 cut(s) 288, 356, 616, 754, 875
SspMI CTAG 2 cut(s) 494, 822
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 432
StyI CCWWGG 4 cut(s) 183, 438, 685, 998
TaaI ACNGT 6 cut(s) 364, 455, 536, 869, 882, 940
TaiI ACGT 1 cut(s) 613
TaqI TCGA 1 cut(s) 207
TasI AATT 4 cut(s) 16, 691, 704, 1048
TatI WGTACW 1 cut(s) 496
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 1 cut(s) 561
Tru1I TTAA 4 cut(s) 240, 759, 764, 1004
Tru9I TTAA 4 cut(s) 240, 759, 764, 1004
TscAI CASTG 6 cut(s) 460, 489, 840, 874, 887, 943
TseFI GTSAC 3 cut(s) 161, 530, 673
TseI GCWGC 2 cut(s) 299, 923
Tsp45I GTSAC 3 cut(s) 161, 530, 673
TspDTI ATGAA 1 cut(s) 462
TspRI CASTG 6 cut(s) 460, 489, 840, 874, 887, 943
Van91I CCANNNNNTGG 1 cut(s) 439
XceI RCATGY 2 cut(s) 919, 923
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 2 cut(s) 494, 822
ZrmI AGTACT 1 cut(s) 498
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.