Rroxscaffold_5G00368350

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015600
Physical Location & Seq
Reverse (-)
50153108 .. 50155424
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00368350.1

Sequence Viewer

Length: 1041 bp
ATGATTGTGAGCCAGAATATGGTGGCTGAGGCCGAGGTTTCGGGCTTCGAATCGGTTGTGAGGTGCTCAGAGACCATTGCTAATGCTGTAATAGAGACTCCTGCAGTCAAGTTTGTTCCACCCATTCACTCAGGAAGCCATAGTGACATCGGAGTAAGGCATTCCATGGATGACGATCACATTCGGATCGATGACCTATCTGCCCATGTTGGGCCTCTCTTTAAGAGTTCCTTGCCAAGTGCATTTTATGCAGTTTTTGATGGGCATGGCGGGCCTGAAGCAGCTACTTATATCAAGAGGAATGCCATGAGACTATTTTTTGAAGATGCTGACTTGCCGCATACGGTAGATTTTGATGATGCTTTCTTCAAAGAGTTGGAGAACTCACATAAGAAAGCATTTTTACTGGCAGACCAGGCCTTGGCCGATGAACACAGTGTGGATGGTTCATGTGGAACCACAGCACTGACTGCCCTAGTACTTGGAAGGCAGTTGCTCGTAGCAAATGCTGGTGACTGTCGGGCAGTTCTTTGCAGGAAAGGAATCGCATTTGATATGTCCCAAGATCATAAGCATACTCACTTGCCAGAACGTAGGCGGGTGGAGCAGTTGGGTGGTTACTTTGTTGATGAGTATCTGAATGGTTATCTCTCAGTCACTCGAACCCTTGGAAATTGGGATTTCAAATTACCACTTGGCTCCTCATCACCTCTCATTGCTGAACCGGAGGTTCGGCGGGTTAAGTTAACAGAAGATGATGAGTTTCTGATTCTTAGTTGTGATGGGATATGGGATGTCATGTCTAGCCAATATGCAGTGAGTCTTGTTCGGCGTGAGCTGAGGAAGCACAATGATCCACAACAGTGTGCCAGAGAACTCGTGAAAGAAGCACTACGCAAGCATGCATATGACAATCTCACTGTTATTGTTGTATGCCTCTCTTCTCCTGATCTTGCTGTTGAGCCTTGCACTGAGGGGCCAAGGTTAAGGAACTGCAGTTTTTCAAGGTTGAGAAGCTTGCTGGAAGGCAATTCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

346

Amino Acids

38.39

Weight (kDa)

5.44

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 45 - 305 1.7e-63 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20910
malus_domestica MD13G1147300.v1.1 MD16G1145700.v1.1
prunus_persica Prupe.1G110800_v2.0.a1
pyrus_communis pycom16g12490
rosa_chinensis RchiOBHm_Chr4g0426341
rosa_laevigata RLG00000007301
rosa_multiflora Rmu_sc0007942.1_g000010 Rmu_sc0020213.1_g000001
rosa_roxburghii Rroxscaffold_5G00368350
rosa_rugosa Rorug04G0210300
rosa_samantha Rh4AG267300 Rh4BG273200 Rh4CG287400 Rh4DG269900
rosa_wichuraiana Rw4G023160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 19, 421
AciI CCGC 4 cut(s) 270, 338, 598, 736
AclWI GGATC 2 cut(s) 194, 848
AcoI YGGCCR 1 cut(s) 423
AcuI CTGAAG 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 439
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 3 cut(s) 19, 210, 421
AgsI TTSAA 5 cut(s) 323, 370, 685, 1005, 1035
AjnI CCWGG 1 cut(s) 414
AleI CACNNNNGTG 1 cut(s) 862
AluBI AGCT 3 cut(s) 284, 838, 1017
AluI AGCT 3 cut(s) 284, 838, 1017
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 3 cut(s) 65, 89, 304
AlwI GGATC 2 cut(s) 194, 848
AoxI GGCC 6 cut(s) 30, 212, 272, 417, 423, 977
ApeKI GCWGC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 212, 272, 977
AsuHPI GGTGA 2 cut(s) 524, 699
AsuII TTCGAA 1 cut(s) 48
BauI CACGAG 1 cut(s) 878
Bbv12I GWGCWC 1 cut(s) 68
BbvCI CCTCAGC 2 cut(s) 27, 839
BbvI GCAGC 1 cut(s) 293
BccI CCATC 3 cut(s) 254, 437, 776
BciT130I CCWGG 1 cut(s) 416
BcoDI GTCTC 3 cut(s) 65, 89, 304
BfaI CTAG 2 cut(s) 476, 804
BfmI CTRYAG 2 cut(s) 102, 994
BisI GCNGC 2 cut(s) 282, 338
BlsI GCNGC 2 cut(s) 283, 339
BmcAI AGTACT 1 cut(s) 480
Bme1390I CCNGG 1 cut(s) 416
BmgT120I GGNCC 3 cut(s) 212, 272, 977
BmiI GGNNCC 3 cut(s) 457, 700, 978
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 2 cut(s) 316, 349
Bpu10I CCTNAGC 2 cut(s) 27, 839
Bpu14I TTCGAA 1 cut(s) 48
Bsa29I ATCGAT 1 cut(s) 189
BsaBI GATNNNNATC 2 cut(s) 174, 633
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 5 cut(s) 33, 165, 420, 667, 980
BsaWI WCCGGW 1 cut(s) 724
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 210, 421
Bse1I ACTGG 1 cut(s) 411
Bse3DI GCAATG 2 cut(s) 75, 714
Bse8I GATNNNNATC 2 cut(s) 174, 633
BseBI CCWGG 1 cut(s) 416
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 5 cut(s) 33, 165, 420, 667, 980
BseGI GGATG 3 cut(s) 175, 448, 799
BseJI GATNNNNATC 2 cut(s) 174, 633
BseLI CCNNNNNNNGG 3 cut(s) 19, 210, 421
BseMI GCAATG 2 cut(s) 75, 714
BseMII CTCAG 6 cut(s) 18, 81, 144, 666, 830, 963
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 691
BseXI GCAGC 1 cut(s) 293
BshFI GGCC 6 cut(s) 32, 214, 274, 419, 425, 979
BshVI ATCGAT 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 68
BsiSI CCGG 1 cut(s) 725
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 3 cut(s) 19, 210, 421
BsmAI GTCTC 3 cut(s) 65, 89, 304
BsmFI GGGAC 1 cut(s) 544
BsmI GAATGC 2 cut(s) 160, 307
BsnI GGCC 6 cut(s) 32, 214, 274, 419, 425, 979
Bso31I GGTCTC 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 68
Bsp143I GATC 5 cut(s) 175, 186, 565, 853, 949
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 4 cut(s) 270, 338, 598, 736
BspANI GGCC 6 cut(s) 32, 214, 274, 419, 425, 979
BspCNI CTCAG 6 cut(s) 19, 80, 143, 665, 831, 964
BspDI ATCGAT 1 cut(s) 189
BspLI GGNNCC 3 cut(s) 457, 700, 978
BspMAI CTGCAG 2 cut(s) 106, 998
BspPI GGATC 2 cut(s) 194, 848
BspT104I TTCGAA 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 2 cut(s) 75, 714
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 5 cut(s) 33, 165, 420, 667, 980
BssMI GATC 5 cut(s) 175, 186, 565, 853, 949
BssSI CACGAG 1 cut(s) 878
BssT1I CCWWGG 4 cut(s) 165, 420, 667, 980
Bst2BI CACGAG 1 cut(s) 878
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 5 cut(s) 346, 437, 518, 864, 922
Bst6I CTCTTC 1 cut(s) 946
BstAPI GCANNNNNTGC 2 cut(s) 248, 470
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 4 cut(s) 272, 899, 903, 1019
BstDEI CTNAG 7 cut(s) 27, 67, 130, 652, 773, 839, 972
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 175, 448, 799
BstKTI GATC 5 cut(s) 178, 189, 568, 856, 952
BstMAI GTCTC 3 cut(s) 65, 89, 304
BstMBI GATC 5 cut(s) 175, 186, 565, 853, 949
BstMWI GCNNNNNNNGC 6 cut(s) 248, 271, 416, 470, 604, 844
BstNI CCWGG 1 cut(s) 416
BstNSI RCATGY 1 cut(s) 905
BstSCI CCNGG 1 cut(s) 414
BstSFI CTRYAG 2 cut(s) 102, 994
BstV1I GCAGC 1 cut(s) 293
Bsu15I ATCGAT 1 cut(s) 189
BsuRI GGCC 6 cut(s) 32, 214, 274, 419, 425, 979
BsuTUI ATCGAT 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 3 cut(s) 175, 448, 799
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 6 cut(s) 442, 464, 822, 869, 918, 969
Cac8I GCNNGC 4 cut(s) 272, 899, 903, 1019
Cfr13I GGNCC 3 cut(s) 212, 272, 977
ClaI ATCGAT 1 cut(s) 189
Csp6I GTAC 1 cut(s) 479
CviAII CATG 7 cut(s) 166, 206, 266, 307, 450, 799, 902
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 7 cut(s) 27, 67, 130, 652, 773, 839, 972
DpnI GATC 5 cut(s) 177, 188, 567, 855, 951
DpnII GATC 5 cut(s) 175, 186, 565, 853, 949
DraIII CACNNNGTG 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 946
EarI CTCTTC 1 cut(s) 946
Eco130I CCWWGG 4 cut(s) 165, 420, 667, 980
Eco147I AGGCCT 1 cut(s) 419
Eco31I GGTCTC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 414
EcoT14I CCWWGG 4 cut(s) 165, 420, 667, 980
EcoT22I ATGCAT 1 cut(s) 907
ErhI CCWWGG 4 cut(s) 165, 420, 667, 980
FaeI CATG 7 cut(s) 169, 209, 269, 310, 453, 802, 905
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 1 cut(s) 544
FatI CATG 7 cut(s) 165, 205, 265, 306, 449, 798, 901
FauI CCCGC 3 cut(s) 263, 591, 729
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 2 cut(s) 282, 338
FokI GGATG 3 cut(s) 182, 455, 806
Fsp4HI GCNGC 2 cut(s) 282, 338
FspBI CTAG 2 cut(s) 476, 804
GluI GCNGC 2 cut(s) 282, 338
HaeIII GGCC 6 cut(s) 32, 214, 274, 419, 425, 979
HapII CCGG 1 cut(s) 725
Hin1II CATG 7 cut(s) 169, 209, 269, 310, 453, 802, 905
HincII GTYRAC 1 cut(s) 747
HindII GTYRAC 1 cut(s) 747
HindIII AAGCTT 1 cut(s) 1015
HinfI GANTC 5 cut(s) 50, 97, 543, 769, 820
HpaI GTTAAC 1 cut(s) 747
HpaII CCGG 1 cut(s) 725
HphI GGTGA 2 cut(s) 524, 699
Hpy166II GTNNAC 1 cut(s) 747
Hpy188I TCNGA 5 cut(s) 70, 152, 186, 639, 768
Hpy188III TCNNGA 4 cut(s) 132, 295, 880, 947
Hpy8I GTNNAC 1 cut(s) 747
HpyAV CCTTC 2 cut(s) 480, 1019
HpyCH4III ACNGT 5 cut(s) 346, 437, 518, 864, 922
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 8 cut(s) 104, 242, 251, 534, 815, 905, 969, 996
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 271, 416, 470, 604, 844
HpyF3I CTNAG 7 cut(s) 27, 67, 130, 652, 773, 839, 972
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 7 cut(s) 169, 209, 269, 310, 453, 802, 905
KspAI GTTAAC 1 cut(s) 747
Kzo9I GATC 5 cut(s) 175, 186, 565, 853, 949
LmnI GCTCC 2 cut(s) 604, 704
Lsp1109I GCAGC 1 cut(s) 293
LweI GCATC 2 cut(s) 316, 349
MaeI CTAG 2 cut(s) 476, 804
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 4 cut(s) 143, 512, 617, 655
MalI GATC 5 cut(s) 177, 188, 567, 855, 951
MboI GATC 5 cut(s) 175, 186, 565, 853, 949
MboII GAAGA 4 cut(s) 335, 358, 764, 933
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 3 cut(s) 673, 686, 1030
MlyI GAGTC 2 cut(s) 91, 829
MmeI TCCRAC 1 cut(s) 357
Mph1103I ATGCAT 1 cut(s) 907
MseI TTAA 4 cut(s) 222, 741, 746, 986
MslI CAYNNNNRTG 2 cut(s) 862, 906
MspI CCGG 1 cut(s) 725
MspR9I CCNGG 1 cut(s) 416
Mva1269I GAATGC 2 cut(s) 160, 307
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 6 cut(s) 248, 271, 416, 470, 604, 844
NcoI CCATGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 907
NdeII GATC 5 cut(s) 175, 186, 565, 853, 949
NlaIII CATG 7 cut(s) 169, 209, 269, 310, 453, 802, 905
NlaIV GGNNCC 3 cut(s) 457, 700, 978
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 3 cut(s) 143, 512, 655
NsiI ATGCAT 1 cut(s) 907
NspI RCATGY 1 cut(s) 905
NspV TTCGAA 1 cut(s) 48
OliI CACNNNNGTG 1 cut(s) 862
PaeI GCATGC 1 cut(s) 905
PceI AGGCCT 1 cut(s) 419
PctI GAATGC 2 cut(s) 160, 307
PfeI GAWTC 3 cut(s) 50, 543, 769
PflMI CCANNNNNTGG 2 cut(s) 19, 421
PkrI GCNGC 2 cut(s) 283, 339
PleI GAGTC 2 cut(s) 91, 828
PpsI GAGTC 2 cut(s) 91, 828
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 3 cut(s) 457, 700, 978
PspPI GGNCC 3 cut(s) 212, 272, 977
PstI CTGCAG 2 cut(s) 106, 998
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 2 cut(s) 862, 906
SaqAI TTAA 4 cut(s) 222, 741, 746, 986
SatI GCNGC 2 cut(s) 282, 338
Sau3AI GATC 5 cut(s) 175, 186, 565, 853, 949
Sau96I GGNCC 3 cut(s) 212, 272, 977
ScaI AGTACT 1 cut(s) 480
SchI GAGTC 2 cut(s) 91, 829
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 68
SfaNI GCATC 2 cut(s) 316, 349
SfcI CTRYAG 2 cut(s) 102, 994
SfuI TTCGAA 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 862, 906
SphI GCATGC 1 cut(s) 905
Sse9I AATT 3 cut(s) 673, 686, 1030
SseBI AGGCCT 1 cut(s) 419
SsiI CCGC 4 cut(s) 270, 338, 598, 736
SspMI CTAG 2 cut(s) 476, 804
StuI AGGCCT 1 cut(s) 419
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 4 cut(s) 165, 420, 667, 980
TaaI ACNGT 5 cut(s) 346, 437, 518, 864, 922
TaiI ACGT 1 cut(s) 595
TaqI TCGA 3 cut(s) 48, 189, 661
TasI AATT 3 cut(s) 673, 686, 1030
TatI WGTACW 1 cut(s) 478
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 3 cut(s) 50, 543, 769
Tru1I TTAA 4 cut(s) 222, 741, 746, 986
Tru9I TTAA 4 cut(s) 222, 741, 746, 986
TscAI CASTG 6 cut(s) 442, 471, 822, 869, 925, 976
TseFI GTSAC 3 cut(s) 143, 512, 655
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 3 cut(s) 143, 512, 655
TspDTI ATGAA 2 cut(s) 438, 444
TspRI CASTG 6 cut(s) 442, 471, 822, 869, 925, 976
Van91I CCANNNNNTGG 2 cut(s) 19, 421
XceI RCATGY 1 cut(s) 905
XspI CTAG 2 cut(s) 476, 804
ZrmI AGTACT 1 cut(s) 480
Zsp2I ATGCAT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.