FvH4_4g21260

Proteasome assembly chaperone 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014519
Physical Location & Seq
Forward (+)
24337707 .. 24339214
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21260.t1

Sequence Viewer

Length: 471 bp
ATGGCTAGCGGAGACTCGGACCTGGGTGCTCTAACCCAAGCTTTAAGCGCTTCCCATATGTCCAAAGAGAAGCAACCCAGCTACAGCAACGGAGACGCCGACGCTGGTGGCGTGCAAGTCACGTGCTTCACTGAGGTCGTCCATGATTCCACTACCCTCCACTTCCAAATCATCCGCCTTCCAAAGCAGATATATGCATGGATTGGTTGCAACTCTGGAAAATTGGGGCATTTGTATGCAGCAGCACCCACTCGACCTAGTACCGTGAGTGTTACATCCATAGTTGGTGGGAGTTCTGATAATACAGGGTCGGGCATTGCTCGGAGATTGGTGTCGAGGACTGGACTAAACATAATTCTGGCTTGCAACATTCCTAAGAATAATCCCATGGTTGAGTTGGATGCTGAAAAATTGTTGGTTCAGAAGCTTCTCAGTCTTGGATACATAAGGCCAAAATCACAAGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC4

Protein Analysis

157

Amino Acids

16.47

Weight (kDa)

8.84

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC4 PF16093 52 - 124 6.3e-20 Proteasome assembly chaperone 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48170
fragaria_vesca FvH4_4g21260
malus_domestica MD13G1143600.v1.1 MD16G1146600.v1.1
prunus_persica Prupe.1G108100_v2.0.a1
pyrus_communis pycom13g12450
rosa_chinensis RchiOBHm_Chr4g0426881
rosa_laevigata RLG00000007271
rosa_roxburghii Rroxscaffold_5G00368850 Rroxscaffold_5G00379210
rosa_samantha Rh2DG030000 Rh4AG270600 Rh4BG276900 Rh4CG291600 Rh4DG274100
rosa_wichuraiana Rw4G023480 Rw5G000420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 9, 175
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 1 cut(s) 262
AfeI AGCGCT 1 cut(s) 49
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 3 cut(s) 41, 81, 427
AluI AGCT 3 cut(s) 41, 81, 427
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 2 cut(s) 6, 87
Aor51HI AGCGCT 1 cut(s) 49
AoxI GGCC 1 cut(s) 449
ApeKI GCWGC 2 cut(s) 239, 242
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 19
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 19
BbrPI CACGTG 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 2 cut(s) 251, 254
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 1 cut(s) 434
BcoDI GTCTC 2 cut(s) 6, 87
BfaI CTAG 2 cut(s) 6, 258
BfmI CTRYAG 1 cut(s) 82
BfoI RGCGCY 1 cut(s) 51
BfuI GTATCC 1 cut(s) 434
BisI GCNGC 2 cut(s) 240, 243
BlsI GCNGC 2 cut(s) 241, 244
Bme1390I CCNGG 1 cut(s) 23
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 1 cut(s) 391
BmtI GCTAGC 1 cut(s) 9
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 22, 387
Bse1I ACTGG 1 cut(s) 346
Bse3DI GCAATG 1 cut(s) 315
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 22, 387
BseGI GGATG 3 cut(s) 171, 275, 406
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 2 cut(s) 123, 445
BseNI ACTGG 1 cut(s) 346
BseXI GCAGC 2 cut(s) 251, 254
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 1 cut(s) 451
BsiHKAI GWGCWC 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 6, 87
BsmBI CGTCTC 1 cut(s) 87
BsnI GGCC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 31
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 2 cut(s) 9, 175
BspANI GGCC 1 cut(s) 451
BspCNI CTCAG 2 cut(s) 124, 444
BspOI GCTAGC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 22, 387
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 265
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 7, 113, 364
BstDEI CTNAG 3 cut(s) 132, 375, 431
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 171, 275, 406
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstMAI GTCTC 2 cut(s) 6, 87
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 251, 254
BsuI GTATCC 1 cut(s) 434
BsuRI GGCC 1 cut(s) 451
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 3 cut(s) 171, 275, 406
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 3 cut(s) 7, 113, 364
CfoI GCGC 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 19
CseI GACGC 2 cut(s) 104, 110
Csp6I GTAC 1 cut(s) 261
CviAII CATG 3 cut(s) 143, 198, 388
CviJI RGCY 6 cut(s) 5, 41, 81, 362, 427, 451
CviKI_1 RGCY 6 cut(s) 5, 41, 81, 362, 427, 451
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 3 cut(s) 132, 375, 431
EciI GGCGGA 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 19
Eco47III AGCGCT 1 cut(s) 49
Eco72I CACGTG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 387
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 3 cut(s) 146, 201, 391
FatI CATG 3 cut(s) 142, 197, 387
FauNDI CATATG 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 240, 243
FokI GGATG 3 cut(s) 158, 262, 413
Fsp4HI GCNGC 2 cut(s) 240, 243
FspBI CTAG 2 cut(s) 6, 258
GlaI GCGC 1 cut(s) 49
GluI GCNGC 2 cut(s) 240, 243
GsaI CCCAGC 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 1 cut(s) 451
HgaI GACGC 2 cut(s) 104, 110
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 3 cut(s) 146, 201, 391
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HindIII AAGCTT 2 cut(s) 39, 425
HinfI GANTC 2 cut(s) 14, 146
Hpy188I TCNGA 4 cut(s) 19, 298, 324, 423
Hpy188III TCNNGA 1 cut(s) 216
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 5 cut(s) 115, 197, 210, 239, 366
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 3 cut(s) 132, 375, 431
HpySE526I ACGT 1 cut(s) 122
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 3 cut(s) 146, 201, 391
HspAI GCGC 1 cut(s) 48
LpnPI CCDG 8 cut(s) 8, 35, 90, 91, 201, 291, 327, 344
Lsp1109I GCAGC 2 cut(s) 251, 254
LweI GCATC 1 cut(s) 391
MaeI CTAG 2 cut(s) 6, 258
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 2 cut(s) 118, 271
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 3 cut(s) 221, 354, 410
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 3 cut(s) 127, 167, 330
Mph1103I ATGCAT 1 cut(s) 199
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 47
NcoI CCATGG 1 cut(s) 387
NdeI CATATG 1 cut(s) 57
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 3 cut(s) 146, 201, 391
NmuCI GTSAC 1 cut(s) 118
NsiI ATGCAT 1 cut(s) 199
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 108
PfeI GAWTC 1 cut(s) 146
PkrI GCNGC 2 cut(s) 241, 244
PleI GAGTC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 21
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 1 cut(s) 21
PspPI GGNCC 1 cut(s) 19
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 2 cut(s) 240, 243
Sau96I GGNCC 1 cut(s) 19
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 31
SetI ASST 7 cut(s) 24, 43, 83, 125, 138, 259, 429
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 82
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 234
Sse9I AATT 3 cut(s) 221, 354, 410
SsiI CCGC 2 cut(s) 9, 175
SspMI CTAG 2 cut(s) 6, 258
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 1 cut(s) 125
TaqI TCGA 2 cut(s) 253, 335
TasI AATT 3 cut(s) 221, 354, 410
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 2 cut(s) 239, 242
Tsp45I GTSAC 1 cut(s) 118
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XspI CTAG 2 cut(s) 6, 258
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.