RchiOBHm_Chr4g0426881

Proteasome assembly chaperone 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014519
Physical Location & Seq
Forward (+)
52216898 .. 52218335
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39586

Sequence Viewer

Length: 480 bp
ATGGCTCACGGAGATTCGAGCCTGGGTGCTCTAAGCGAAGCTTTAAGCTCTTCTCAAATTTCCAAAGAGAAACAACCCACTGATCCCACCTACAGCAATGGAGACGACGACGCTGGTGGCGTGCAGATCACGTGCTTCACTGAGCTTGTCCATGATTCTACTACCCTCCACTTCCAAATCATCCGCCTTCCAAAGCAGATATATGTATGGATTGGTTGCAGCTCTGGAAAATTGGGGCATTTGTATGCAGCAGCACCCACTCGACCTAGTACAGTGAGTGTTACATCCATACTTGGTGGGAGTTCTGATAATACAGGGTCGGGCATTGCTAGGAGATTGGTGTCGAGGACTGGACTAAACATAATTCTGGCTTGCAACATTCCTAAGAATAATCCCATGATTGAGTTGGATGCTGAGAAGTTGTTGGTTCAGAAGCTACTCAATCTTGGGTACATAAGGCCAAAGTCACAAGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC4

Protein Analysis

159

Amino Acids

16.92

Weight (kDa)

7.74

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC4 PF16093 55 - 127 7.9e-21 Proteasome assembly chaperone 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0014519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48170
fragaria_vesca FvH4_4g21260
malus_domestica MD13G1143600.v1.1 MD16G1146600.v1.1
prunus_persica Prupe.1G108100_v2.0.a1
pyrus_communis pycom13g12450
rosa_chinensis RchiOBHm_Chr4g0426881
rosa_laevigata RLG00000007271
rosa_roxburghii Rroxscaffold_5G00368850 Rroxscaffold_5G00379210
rosa_samantha Rh2DG030000 Rh4AG270600 Rh4BG276900 Rh4CG291600 Rh4DG274100
rosa_wichuraiana Rw4G023480 Rw5G000420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 184
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 1 cut(s) 57
AcvI CACGTG 1 cut(s) 132
AfaI GTAC 2 cut(s) 271, 452
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 5 cut(s) 41, 48, 145, 222, 436
AluI AGCT 5 cut(s) 41, 48, 145, 222, 436
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 1 cut(s) 77
AoxI GGCC 1 cut(s) 458
ApeKI GCWGC 3 cut(s) 219, 248, 251
ApoI RAATTY 1 cut(s) 57
BbrPI CACGTG 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 3 cut(s) 231, 260, 263
BciT130I CCWGG 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 2 cut(s) 267, 330
BfmI CTRYAG 1 cut(s) 91
BisI GCNGC 3 cut(s) 220, 249, 252
BlsI GCNGC 3 cut(s) 221, 250, 253
Bme1390I CCNGG 1 cut(s) 23
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 1 cut(s) 400
BsaAI YACGTR 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 2 cut(s) 103, 324
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 3 cut(s) 180, 284, 415
BseMI GCAATG 2 cut(s) 103, 324
BseMII CTCAG 2 cut(s) 132, 405
BseNI ACTGG 1 cut(s) 355
BseXI GCAGC 3 cut(s) 231, 260, 263
BsgI GTGCAG 1 cut(s) 143
BshFI GGCC 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 31
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsnI GGCC 1 cut(s) 460
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 2 cut(s) 82, 126
BspACI CCGC 1 cut(s) 184
BspANI GGCC 1 cut(s) 460
BspCNI CTCAG 2 cut(s) 133, 406
BspPI GGATC 1 cut(s) 77
BspQI GCTCTTC 1 cut(s) 55
BsrDI GCAATG 2 cut(s) 103, 324
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 2 cut(s) 82, 126
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 55
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 122, 373
BstDEI CTNAG 4 cut(s) 32, 141, 384, 414
BstF5I GGATG 3 cut(s) 180, 284, 415
BstKTI GATC 2 cut(s) 85, 129
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 2 cut(s) 82, 126
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 3 cut(s) 231, 260, 263
BsuRI GGCC 1 cut(s) 460
BtsCI GGATG 3 cut(s) 180, 284, 415
BtsIMutI CAGTG 3 cut(s) 78, 138, 279
Cac8I GCNNGC 2 cut(s) 122, 373
CseI GACGC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 270, 451
CviAII CATG 2 cut(s) 152, 397
CviJI RGCY 9 cut(s) 5, 21, 41, 48, 145, 222, 371, 436, 460
CviKI_1 RGCY 9 cut(s) 5, 21, 41, 48, 145, 222, 371, 436, 460
CviQI GTAC 2 cut(s) 270, 451
DdeI CTNAG 4 cut(s) 32, 141, 384, 414
DpnI GATC 2 cut(s) 84, 128
DpnII GATC 2 cut(s) 82, 126
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 173
Eco72I CACGTG 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 2 cut(s) 155, 400
FaiI YATR 9 cut(s) 153, 202, 204, 208, 246, 290, 362, 398, 455
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FatI CATG 2 cut(s) 151, 396
Fnu4HI GCNGC 3 cut(s) 220, 249, 252
FokI GGATG 3 cut(s) 167, 271, 422
Fsp4HI GCNGC 3 cut(s) 220, 249, 252
FspBI CTAG 2 cut(s) 267, 330
GluI GCNGC 3 cut(s) 220, 249, 252
HaeIII GGCC 1 cut(s) 460
HgaI GACGC 1 cut(s) 119
Hin1II CATG 2 cut(s) 155, 400
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 2 cut(s) 14, 155
Hpy188I TCNGA 2 cut(s) 307, 432
Hpy188III TCNNGA 1 cut(s) 225
Hpy99I CGWCG 2 cut(s) 110, 113
HpyAV CCTTC 1 cut(s) 197
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 4 cut(s) 124, 219, 248, 375
HpyF3I CTNAG 4 cut(s) 32, 141, 384, 414
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 2 cut(s) 155, 400
Kzo9I GATC 2 cut(s) 82, 126
LguI GCTCTTC 1 cut(s) 55
LpnPI CCDG 7 cut(s) 8, 35, 99, 210, 300, 336, 353
Lsp1109I GCAGC 3 cut(s) 231, 260, 263
LweI GCATC 1 cut(s) 400
MaeI CTAG 2 cut(s) 267, 330
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 280, 465
MalI GATC 2 cut(s) 84, 128
MboI GATC 2 cut(s) 82, 126
MboII GAAGA 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 3 cut(s) 57, 230, 363
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 2 cut(s) 176, 339
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
NdeII GATC 2 cut(s) 82, 126
NlaIII CATG 2 cut(s) 155, 400
NmuCI GTSAC 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 2 cut(s) 14, 155
PkrI GCNGC 3 cut(s) 221, 250, 253
PmaCI CACGTG 1 cut(s) 132
PmlI CACGTG 1 cut(s) 132
Ppu21I YACGTR 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 21
PspCI CACGTG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 21
RsaI GTAC 2 cut(s) 271, 452
RsaNI GTAC 2 cut(s) 270, 451
RseI CAYNNNNRTG 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 3 cut(s) 220, 249, 252
Sau3AI GATC 2 cut(s) 82, 126
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 31
SetI ASST 8 cut(s) 43, 50, 92, 134, 147, 224, 268, 438
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 243
Sse9I AATT 3 cut(s) 57, 230, 363
SsiI CCGC 1 cut(s) 184
SspMI CTAG 2 cut(s) 267, 330
StyD4I CCNGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 134
TaqI TCGA 3 cut(s) 17, 262, 344
TasI AATT 3 cut(s) 57, 230, 363
TatI WGTACW 1 cut(s) 269
TfiI GAWTC 2 cut(s) 14, 155
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 3 cut(s) 85, 145, 279
TseFI GTSAC 1 cut(s) 465
TseI GCWGC 3 cut(s) 219, 248, 251
Tsp45I GTSAC 1 cut(s) 465
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 3 cut(s) 85, 145, 279
XapI RAATTY 1 cut(s) 57
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XspI CTAG 2 cut(s) 267, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.