Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014519

Physical Location & Seq

Genomic Coordinates Reverse (-)

11432996 .. 11434514

Gene Structure 1519 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1146600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 435 bp

ATGGCGCATGGTAGTTTAGACATCGGCGCTCCAAACAGTGCGTTTAGCTCCGCTCAAATTTCCGAACACAAAGAATCGACTGGTCTGAGCTACATCGACAACGACGACAACGACGATGCCGGTGGCGTGCAAGTCACGTGCTTCAGTGAGGTCGTCAACGAAACCACCTTCCACCTCCAAATCATCCGCCTTCCCAAGCAGATATATGCATGGATTGGTTGCAACTCTACAAAATTTGGGCATTTGTATGCAGCAGCACCTATGAGACCTAACAGTACAGTGGGTGTTACATCCATACTCGGTGGGGCTTCTGATAATACAGGGTCGGGCATTGCTCGAAGATTAGGAATGGATTCGTCTGCAGATGCTGAAAAATTGTTGGTTCAGAAGCTAATCAATCTTGGGTACACAAGGCCAAAGTCCGAAGGATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009987 GO:0010033 GO:0016787 GO:0019538 GO:0019783 GO:0023052 GO:0032870 GO:0036459 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070011 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0101005 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PAC4

Protein Analysis

145

Amino Acids

15.29

Weight (kDa)

5.44

Isoelectric Point (pI)

31.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAC4	PF16093	56 - 116	4.2e-13	Proteasome assembly chaperone 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014519)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48170
fragaria_vesca	FvH4_4g21260
malus_domestica	MD13G1143600.v1.1 MD16G1146600.v1.1
prunus_persica	Prupe.1G108100_v2.0.a1
pyrus_communis	pycom13g12450
rosa_chinensis	RchiOBHm_Chr4g0426881
rosa_laevigata	RLG00000007271
rosa_roxburghii	Rroxscaffold_5G00368850 Rroxscaffold_5G00379210
rosa_samantha	Rh2DG030000 Rh4AG270600 Rh4BG276900 Rh4CG291600 Rh4DG274100
rosa_wichuraiana	Rw4G023480 Rw5G000420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	53
AciI	CCGC	2 cut(s)	51, 187
AcsI	RAATTY	2 cut(s)	57, 233
AcuI	CTGAAG	1 cut(s)	127
AcvI	CACGTG	1 cut(s)	138
AfaI	GTAC	2 cut(s)	277, 407
AluBI	AGCT	3 cut(s)	48, 90, 391
AluI	AGCT	3 cut(s)	48, 90, 391
Alw26I	GTCTC	1 cut(s)	259
AlwNI	CAGNNNCTG	1 cut(s)	368
AoxI	GGCC	1 cut(s)	413
ApeKI	GCWGC	2 cut(s)	251, 254
ApoI	RAATTY	2 cut(s)	57, 233
Asp700I	GAANNNNTTC	1 cut(s)	352
AspLEI	GCGC	2 cut(s)	7, 29
BbrPI	CACGTG	1 cut(s)	138
BbvI	GCAGC	2 cut(s)	263, 266
BcoDI	GTCTC	1 cut(s)	259
BfaI	CTAG	1 cut(s)	433
BfmI	CTRYAG	1 cut(s)	360
BfoI	RGCGCY	1 cut(s)	30
BisI	GCNGC	2 cut(s)	252, 255
BlsI	GCNGC	2 cut(s)	253, 256
BmsI	GCATC	2 cut(s)	106, 355
BsaAI	YACGTR	1 cut(s)	138
BsaI	GGTCTC	1 cut(s)	259
Bse118I	RCCGGY	1 cut(s)	119
Bse1I	ACTGG	1 cut(s)	85
Bse3DI	GCAATG	1 cut(s)	330
BseGI	GGATG	2 cut(s)	183, 290
BseMI	GCAATG	1 cut(s)	330
BseMII	CTCAG	1 cut(s)	77
BseNI	ACTGG	1 cut(s)	85
BseXI	GCAGC	2 cut(s)	263, 266
BshFI	GGCC	1 cut(s)	415
BsiSI	CCGG	1 cut(s)	120
BsmAI	GTCTC	1 cut(s)	259
BsnI	GGCC	1 cut(s)	415
Bso31I	GGTCTC	1 cut(s)	259
BspACI	CCGC	2 cut(s)	51, 187
BspANI	GGCC	1 cut(s)	415
BspCNI	CTCAG	1 cut(s)	78
BspMAI	CTGCAG	1 cut(s)	364
BspTNI	GGTCTC	1 cut(s)	259
BsrBI	CCGCTC	1 cut(s)	53
BsrDI	GCAATG	1 cut(s)	330
BsrFI	RCCGGY	1 cut(s)	119
BsrI	ACTGG	1 cut(s)	85
BssAI	RCCGGY	1 cut(s)	119
Bst4CI	ACNGT	3 cut(s)	38, 275, 280
BstBAI	YACGTR	1 cut(s)	138
BstC8I	GCNNGC	1 cut(s)	128
BstDEI	CTNAG	1 cut(s)	86
BstF5I	GGATG	2 cut(s)	183, 290
BstH2I	RGCGCY	1 cut(s)	30
BstHHI	GCGC	2 cut(s)	7, 29
BstMAI	GTCTC	1 cut(s)	259
BstSFI	CTRYAG	1 cut(s)	360
BstV1I	GCAGC	2 cut(s)	263, 266
BsuRI	GGCC	1 cut(s)	415
BtsCI	GGATG	2 cut(s)	183, 290
BtsIMutI	CAGTG	3 cut(s)	43, 151, 285
Cac8I	GCNNGC	1 cut(s)	128
CaiI	CAGNNNCTG	1 cut(s)	368
CfoI	GCGC	2 cut(s)	7, 29
Cfr10I	RCCGGY	1 cut(s)	119
Csp6I	GTAC	2 cut(s)	276, 406
CviAII	CATG	2 cut(s)	8, 210
CviJI	RGCY	5 cut(s)	48, 90, 308, 391, 415
CviKI_1	RGCY	5 cut(s)	48, 90, 308, 391, 415
CviQI	GTAC	2 cut(s)	276, 406
DdeI	CTNAG	1 cut(s)	86
EciI	GGCGGA	1 cut(s)	176
Eco31I	GGTCTC	1 cut(s)	259
Eco57I	CTGAAG	1 cut(s)	127
Eco72I	CACGTG	1 cut(s)	138
EcoT22I	ATGCAT	1 cut(s)	211
FaeI	CATG	2 cut(s)	11, 213
FaiI	YATR	7 cut(s)	9, 205, 207, 211, 249, 263, 296
FatI	CATG	2 cut(s)	7, 209
Fnu4HI	GCNGC	2 cut(s)	252, 255
FokI	GGATG	2 cut(s)	170, 277
Fsp4HI	GCNGC	2 cut(s)	252, 255
FspBI	CTAG	1 cut(s)	433
GlaI	GCGC	2 cut(s)	6, 28
GluI	GCNGC	2 cut(s)	252, 255
HaeII	RGCGCY	1 cut(s)	30
HaeIII	GGCC	1 cut(s)	415
HapII	CCGG	1 cut(s)	120
HhaI	GCGC	2 cut(s)	7, 29
Hin1II	CATG	2 cut(s)	11, 213
Hin6I	GCGC	2 cut(s)	5, 27
HinP1I	GCGC	2 cut(s)	5, 27
HincII	GTYRAC	1 cut(s)	157
HindII	GTYRAC	1 cut(s)	157
HinfI	GANTC	3 cut(s)	74, 353, 429
HpaII	CCGG	1 cut(s)	120
Hpy166II	GTNNAC	2 cut(s)	157, 408
Hpy188I	TCNGA	5 cut(s)	64, 87, 313, 387, 424
Hpy8I	GTNNAC	2 cut(s)	157, 408
Hpy99I	CGWCG	2 cut(s)	107, 116
HpyAV	CCTTC	3 cut(s)	178, 200, 419
HpyCH4III	ACNGT	3 cut(s)	38, 275, 280
HpyCH4IV	ACGT	1 cut(s)	137
HpyCH4V	TGCA	5 cut(s)	130, 209, 222, 251, 362
HpyF3I	CTNAG	1 cut(s)	86
HpySE526I	ACGT	1 cut(s)	137
Hsp92II	CATG	2 cut(s)	11, 213
HspAI	GCGC	2 cut(s)	5, 27
LmnI	GCTCC	2 cut(s)	34, 53
LpnPI	CCDG	3 cut(s)	66, 133, 306
Lsp1109I	GCAGC	2 cut(s)	263, 266
LweI	GCATC	2 cut(s)	106, 355
MaeI	CTAG	1 cut(s)	433
MaeII	ACGT	1 cut(s)	137
MaeIII	GTNAC	2 cut(s)	133, 286
MbiI	CCGCTC	1 cut(s)	53
MboII	GAAGA	1 cut(s)	351
MluCI	AATT	3 cut(s)	57, 233, 374
MnlI	CCTC	2 cut(s)	142, 185
Mph1103I	ATGCAT	1 cut(s)	211
MroXI	GAANNNNTTC	1 cut(s)	352
MslI	CAYNNNNRTG	1 cut(s)	246
MspI	CCGG	1 cut(s)	120
NlaIII	CATG	2 cut(s)	11, 213
NmuCI	GTSAC	1 cut(s)	133
NsiI	ATGCAT	1 cut(s)	211
PcsI	WCGNNNNNNNCGW	3 cut(s)	102, 108, 111
PdmI	GAANNNNTTC	1 cut(s)	352
PfeI	GAWTC	3 cut(s)	74, 353, 429
PkrI	GCNGC	2 cut(s)	253, 256
PmaCI	CACGTG	1 cut(s)	138
PmlI	CACGTG	1 cut(s)	138
Ppu21I	YACGTR	1 cut(s)	138
PspCI	CACGTG	1 cut(s)	138
PstI	CTGCAG	1 cut(s)	364
PstNI	CAGNNNCTG	1 cut(s)	368
RsaI	GTAC	2 cut(s)	277, 407
RsaNI	GTAC	2 cut(s)	276, 406
RseI	CAYNNNNRTG	1 cut(s)	246
SatI	GCNGC	2 cut(s)	252, 255
SetI	ASST	9 cut(s)	50, 92, 140, 153, 170, 177, 262, 271, 393
SfaNI	GCATC	2 cut(s)	106, 355
SfcI	CTRYAG	1 cut(s)	360
SmiMI	CAYNNNNRTG	1 cut(s)	246
Sse9I	AATT	3 cut(s)	57, 233, 374
SsiI	CCGC	2 cut(s)	51, 187
SspMI	CTAG	1 cut(s)	433
TaaI	ACNGT	3 cut(s)	38, 275, 280
TaiI	ACGT	1 cut(s)	140
TaqI	TCGA	3 cut(s)	77, 96, 337
TasI	AATT	3 cut(s)	57, 233, 374
TatI	WGTACW	1 cut(s)	275
TfiI	GAWTC	3 cut(s)	74, 353, 429
TscAI	CASTG	3 cut(s)	43, 151, 285
TseFI	GTSAC	1 cut(s)	133
TseI	GCWGC	2 cut(s)	251, 254
Tsp45I	GTSAC	1 cut(s)	133
TspRI	CASTG	3 cut(s)	43, 151, 285
XapI	RAATTY	2 cut(s)	57, 233
XmnI	GAANNNNTTC	1 cut(s)	352
XspI	CTAG	1 cut(s)	433
Zsp2I	ATGCAT	1 cut(s)	211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.