MD13G1143600.v1.1

Proteasome assembly chaperone 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014519
Physical Location & Seq
Reverse (-)
11171132 .. 11172896
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1143600.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGCGCGGAGTGATTTAGACCTCGGCGCTCTAAACGACGCGTTTAGCTCCGCTCAAATTTCCAAACACGAAGAACCGACTGGTCCGAGCTACACCAACGACGACGGCGATGCCGGTGGTTTGCAAATCAGGTGCTTCAGTGAGGTCGTCAACGAAACCGCCTTCCACCTCCAAATCATCCGCCTTCCCAAGCAGATCTATGCATGGATTGGTTGCAACTCTGCAAAAATTGGGCATTTGTATGCAGCAGCACCTATGAGACCTAATACAGTGGGTGTTACATCCATACTTGGAGGGGCTTCTGATAATACAGGTTCGGGCATTGCTCGAAGATTAGTGTTAAAGACTGGACTTAACATAATCCTGGCTTGCAACATTCCTAAGAATAGTCCCATGCTTGAGGCAGATGCTGAAAAATTGTTGGTTCAGAAGCTAATCAACCTTGGGTACACAAGGCCAAGGTCCGGAGGAGTGTCGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC4

Protein Analysis

161

Amino Acids

16.98

Weight (kDa)

7.73

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC4 PF16093 55 - 126 1.8e-17 Proteasome assembly chaperone 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48170
fragaria_vesca FvH4_4g21260
malus_domestica MD13G1143600.v1.1 MD16G1146600.v1.1
prunus_persica Prupe.1G108100_v2.0.a1
pyrus_communis pycom13g12450
rosa_chinensis RchiOBHm_Chr4g0426881
rosa_laevigata RLG00000007271
rosa_roxburghii Rroxscaffold_5G00368850 Rroxscaffold_5G00379210
rosa_samantha Rh2DG030000 Rh4AG270600 Rh4BG276900 Rh4CG291600 Rh4DG274100
rosa_wichuraiana Rw4G023480 Rw5G000420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 53
AccII CGCG 2 cut(s) 7, 41
AccIII TCCGGA 1 cut(s) 464
AciI CCGC 4 cut(s) 7, 51, 159, 181
AcsI RAATTY 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 121
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 1 cut(s) 464
AflIII ACRYGT 1 cut(s) 39
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 3 cut(s) 48, 90, 433
AluI AGCT 3 cut(s) 48, 90, 433
Alw26I GTCTC 1 cut(s) 253
AlwNI CAGNNNCTG 1 cut(s) 410
Aor13HI TCCGGA 1 cut(s) 464
AoxI GGCC 1 cut(s) 455
ApeKI GCWGC 2 cut(s) 245, 248
ApoI RAATTY 1 cut(s) 57
AspLEI GCGC 2 cut(s) 7, 29
AspS9I GGNCC 2 cut(s) 83, 462
AvaII GGWCC 2 cut(s) 83, 462
BbvI GCAGC 2 cut(s) 257, 260
BceAI ACGGC 1 cut(s) 121
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 253
BfoI RGCGCY 1 cut(s) 30
BglII AGATCT 1 cut(s) 195
BisI GCNGC 2 cut(s) 246, 249
BlsI GCNGC 2 cut(s) 247, 250
Bme1390I CCNGG 1 cut(s) 365
Bme18I GGWCC 2 cut(s) 83, 462
BmgT120I GGNCC 2 cut(s) 83, 462
BmrFI CCNGG 1 cut(s) 365
BmsI GCATC 2 cut(s) 100, 397
BpuEI CTTGAG 1 cut(s) 419
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 22, 442, 458
BsaWI WCCGGW 1 cut(s) 464
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 2 cut(s) 85, 352
Bse3DI GCAATG 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 464
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 3 cut(s) 22, 442, 458
BseGI GGATG 2 cut(s) 177, 281
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 85, 352
BseRI GAGGAG 1 cut(s) 483
BseXI GCAGC 2 cut(s) 257, 260
Bsh1236I CGCG 2 cut(s) 7, 41
BshFI GGCC 1 cut(s) 457
BsiSI CCGG 2 cut(s) 114, 465
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 1 cut(s) 457
Bso31I GGTCTC 1 cut(s) 253
Bsp13I TCCGGA 1 cut(s) 464
Bsp143I GATC 1 cut(s) 195
BspACI CCGC 4 cut(s) 7, 51, 159, 181
BspANI GGCC 1 cut(s) 457
BspEI TCCGGA 1 cut(s) 464
BspFNI CGCG 2 cut(s) 7, 41
BspTNI GGTCTC 1 cut(s) 253
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 321
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 2 cut(s) 85, 352
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 22, 442, 458
BssMI GATC 1 cut(s) 195
BssT1I CCWWGG 2 cut(s) 442, 458
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 1 cut(s) 381
BstF5I GGATG 2 cut(s) 177, 281
BstFNI CGCG 2 cut(s) 7, 41
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 2 cut(s) 7, 29
BstKTI GATC 1 cut(s) 198
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 1 cut(s) 195
BstNI CCWGG 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 363
BstUI CGCG 2 cut(s) 7, 41
BstV1I GCAGC 2 cut(s) 257, 260
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BsuRI GGCC 1 cut(s) 457
BtgZI GCGATG 1 cut(s) 123
BtsCI GGATG 2 cut(s) 177, 281
BtsIMutI CAGTG 2 cut(s) 145, 276
Cac8I GCNNGC 1 cut(s) 370
CaiI CAGNNNCTG 1 cut(s) 410
CfoI GCGC 2 cut(s) 7, 29
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 83, 462
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 448
CviAII CATG 2 cut(s) 204, 394
CviJI RGCY 6 cut(s) 48, 90, 299, 368, 433, 457
CviKI_1 RGCY 6 cut(s) 48, 90, 299, 368, 433, 457
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 1 cut(s) 381
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 2 cut(s) 442, 458
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 83, 462
Eco57I CTGAAG 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 2 cut(s) 442, 458
EcoT22I ATGCAT 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 442, 458
FaeI CATG 2 cut(s) 207, 397
FaiI YATR 8 cut(s) 201, 205, 243, 257, 287, 359, 395, 481
FaqI GGGAC 1 cut(s) 375
FatI CATG 2 cut(s) 203, 393
Fnu4HI GCNGC 2 cut(s) 246, 249
FokI GGATG 2 cut(s) 164, 268
Fsp4HI GCNGC 2 cut(s) 246, 249
GlaI GCGC 2 cut(s) 6, 28
GluI GCNGC 2 cut(s) 246, 249
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 1 cut(s) 457
HapII CCGG 2 cut(s) 114, 465
HgaI GACGC 1 cut(s) 47
HhaI GCGC 2 cut(s) 7, 29
Hin1II CATG 2 cut(s) 207, 397
Hin6I GCGC 2 cut(s) 5, 27
HinP1I GCGC 2 cut(s) 5, 27
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HpaII CCGG 2 cut(s) 114, 465
Hpy166II GTNNAC 2 cut(s) 151, 450
Hpy188I TCNGA 3 cut(s) 87, 304, 429
Hpy188III TCNNGA 1 cut(s) 465
Hpy8I GTNNAC 2 cut(s) 151, 450
Hpy99I CGWCG 3 cut(s) 41, 104, 107
HpyAV CCTTC 2 cut(s) 172, 194
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 6 cut(s) 124, 203, 216, 224, 245, 372
HpyF3I CTNAG 1 cut(s) 381
Hsp92II CATG 2 cut(s) 207, 397
HspAI GCGC 2 cut(s) 5, 27
Kpn2I TCCGGA 1 cut(s) 464
Kzo9I GATC 1 cut(s) 195
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 8 cut(s) 66, 115, 127, 297, 333, 350, 377, 478
Lsp1109I GCAGC 2 cut(s) 257, 260
LweI GCATC 2 cut(s) 100, 397
MaeIII GTNAC 1 cut(s) 277
MalI GATC 1 cut(s) 197
MbiI CCGCTC 1 cut(s) 53
MboI GATC 1 cut(s) 195
MboII GAAGA 2 cut(s) 83, 342
MflI RGATCY 1 cut(s) 195
MluCI AATT 3 cut(s) 57, 228, 416
MluI ACGCGT 1 cut(s) 39
MnlI CCTC 6 cut(s) 32, 136, 179, 287, 394, 461
Mph1103I ATGCAT 1 cut(s) 205
MroI TCCGGA 1 cut(s) 464
MseI TTAA 2 cut(s) 341, 354
MslI CAYNNNNRTG 1 cut(s) 240
MspI CCGG 2 cut(s) 114, 465
MspR9I CCNGG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 2 cut(s) 7, 41
NdeII GATC 1 cut(s) 195
NlaIII CATG 2 cut(s) 207, 397
NsiI ATGCAT 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PkrI GCNGC 2 cut(s) 247, 250
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspPI GGNCC 2 cut(s) 83, 462
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 1 cut(s) 195
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 1 cut(s) 240
SaqAI TTAA 2 cut(s) 341, 354
SatI GCNGC 2 cut(s) 246, 249
Sau3AI GATC 1 cut(s) 195
Sau96I GGNCC 2 cut(s) 83, 462
ScrFI CCNGG 1 cut(s) 365
SfaNI GCATC 2 cut(s) 100, 397
SinI GGWCC 2 cut(s) 83, 462
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 3 cut(s) 57, 228, 416
SsiI CCGC 4 cut(s) 7, 51, 159, 181
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 2 cut(s) 442, 458
TaaI ACNGT 1 cut(s) 271
TaqI TCGA 1 cut(s) 328
TasI AATT 3 cut(s) 57, 228, 416
Tru1I TTAA 2 cut(s) 341, 354
Tru9I TTAA 2 cut(s) 341, 354
TscAI CASTG 2 cut(s) 145, 276
TseI GCWGC 2 cut(s) 245, 248
TspRI CASTG 2 cut(s) 145, 276
VpaK11BI GGWCC 2 cut(s) 83, 462
XapI RAATTY 1 cut(s) 57
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.