Rw4G023480

Proteasome assembly chaperone 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014519
Physical Location & Seq
Forward (+)
49076651 .. 49077716
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G023480.1

Sequence Viewer

Length: 444 bp
ATGGCTCACGGAGATTCGAGCCTGGGTGCTCTAAGCGAAGCTTTAAGCTCTTCTCAAATCAATGGAGACGACGACGCTGGTGGCGTGCAGATCACGTGCTTCACTGAGCTTGTCCATGATTCTACTACCCTCCACTTCCAAATCATCCGCCTTCCAAAGCAGATATATGTATGGATTGGTTGCAGCTCTGGAAAATTGGGGCATTTGTATGCAGCAGCACCCACTCGACCTAGTACAGTGAGTGTTACATCCATACTTGGTGGGAGTTCTGATAATACAGGGTCAGGCATTGCTCGGAGATTGGTGTCGAGGACTGGACTAAACATAATTCTGGCTTGCAACATTCCTAAGAATAATCCCATGATTGAGTTGGATGCTGAGAAGTTGTTGGTTCAGAAGCTACTCAATCTTGGGTACGTAAGGCCAAAGTCACAAGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC4

Protein Analysis

147

Amino Acids

15.54

Weight (kDa)

7.76

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC4 PF16093 43 - 115 6.5e-21 Proteasome assembly chaperone 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48170
fragaria_vesca FvH4_4g21260
malus_domestica MD13G1143600.v1.1 MD16G1146600.v1.1
prunus_persica Prupe.1G108100_v2.0.a1
pyrus_communis pycom13g12450
rosa_chinensis RchiOBHm_Chr4g0426881
rosa_laevigata RLG00000007271
rosa_roxburghii Rroxscaffold_5G00368850 Rroxscaffold_5G00379210
rosa_samantha Rh2DG030000 Rh4AG270600 Rh4BG276900 Rh4CG291600 Rh4DG274100
rosa_wichuraiana Rw4G023480 Rw5G000420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 148
AcvI CACGTG 1 cut(s) 96
AfaI GTAC 2 cut(s) 235, 416
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 5 cut(s) 41, 48, 109, 186, 400
AluI AGCT 5 cut(s) 41, 48, 109, 186, 400
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 60
AoxI GGCC 1 cut(s) 422
ApeKI GCWGC 3 cut(s) 183, 212, 215
BbrPI CACGTG 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 3 cut(s) 195, 224, 227
BciT130I CCWGG 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 1 cut(s) 231
BisI GCNGC 3 cut(s) 184, 213, 216
BlsI GCNGC 3 cut(s) 185, 214, 217
Bme1390I CCNGG 1 cut(s) 23
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 1 cut(s) 364
BsaAI YACGTR 2 cut(s) 96, 418
BsaJI CCNNGG 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 3 cut(s) 144, 248, 379
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 2 cut(s) 96, 369
BseNI ACTGG 1 cut(s) 319
BseXI GCAGC 3 cut(s) 195, 224, 227
BsgI GTGCAG 1 cut(s) 107
BshFI GGCC 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 31
BsmAI GTCTC 1 cut(s) 60
BsmBI CGTCTC 1 cut(s) 60
BsnI GGCC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 90
BspACI CCGC 1 cut(s) 148
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 2 cut(s) 97, 370
BspQI GCTCTTC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 55
BstBAI YACGTR 2 cut(s) 96, 418
BstC8I GCNNGC 2 cut(s) 86, 337
BstDEI CTNAG 4 cut(s) 32, 105, 348, 378
BstF5I GGATG 3 cut(s) 144, 248, 379
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 1 cut(s) 90
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstSNI TACGTA 1 cut(s) 418
BstV1I GCAGC 3 cut(s) 195, 224, 227
BsuRI GGCC 1 cut(s) 424
BtsCI GGATG 3 cut(s) 144, 248, 379
BtsIMutI CAGTG 2 cut(s) 102, 243
Cac8I GCNNGC 2 cut(s) 86, 337
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 234, 415
CviAII CATG 2 cut(s) 116, 361
CviJI RGCY 9 cut(s) 5, 21, 41, 48, 109, 186, 335, 400, 424
CviKI_1 RGCY 9 cut(s) 5, 21, 41, 48, 109, 186, 335, 400, 424
CviQI GTAC 2 cut(s) 234, 415
DdeI CTNAG 4 cut(s) 32, 105, 348, 378
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 137
Eco105I TACGTA 1 cut(s) 418
Eco72I CACGTG 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 2 cut(s) 119, 364
FaiI YATR 8 cut(s) 117, 166, 168, 172, 210, 254, 326, 362
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FatI CATG 2 cut(s) 115, 360
Fnu4HI GCNGC 3 cut(s) 184, 213, 216
FokI GGATG 3 cut(s) 131, 235, 386
Fsp4HI GCNGC 3 cut(s) 184, 213, 216
FspBI CTAG 1 cut(s) 231
GluI GCNGC 3 cut(s) 184, 213, 216
HaeIII GGCC 1 cut(s) 424
HgaI GACGC 1 cut(s) 83
Hin1II CATG 2 cut(s) 119, 364
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 2 cut(s) 14, 119
Hpy188I TCNGA 3 cut(s) 271, 297, 396
Hpy188III TCNNGA 1 cut(s) 189
Hpy99I CGWCG 2 cut(s) 74, 77
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 2 cut(s) 95, 417
HpyCH4V TGCA 4 cut(s) 88, 183, 212, 339
HpyF3I CTNAG 4 cut(s) 32, 105, 348, 378
HpySE526I ACGT 2 cut(s) 95, 417
Hsp92II CATG 2 cut(s) 119, 364
Kzo9I GATC 1 cut(s) 90
LguI GCTCTTC 1 cut(s) 55
LpnPI CCDG 8 cut(s) 8, 35, 63, 174, 264, 270, 300, 317
Lsp1109I GCAGC 3 cut(s) 195, 224, 227
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 231
MaeII ACGT 2 cut(s) 95, 417
MaeIII GTNAC 2 cut(s) 244, 429
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 2 cut(s) 194, 327
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 2 cut(s) 140, 303
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
NdeII GATC 1 cut(s) 90
NlaIII CATG 2 cut(s) 119, 364
NmuCI GTSAC 1 cut(s) 429
PciSI GCTCTTC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 2 cut(s) 14, 119
PkrI GCNGC 3 cut(s) 185, 214, 217
PmaCI CACGTG 1 cut(s) 96
PmlI CACGTG 1 cut(s) 96
Ppu21I YACGTR 2 cut(s) 96, 418
Psp6I CCWGG 1 cut(s) 21
PspCI CACGTG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 21
RsaI GTAC 2 cut(s) 235, 416
RsaNI GTAC 2 cut(s) 234, 415
RseI CAYNNNNRTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 3 cut(s) 184, 213, 216
Sau3AI GATC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 31
SetI ASST 8 cut(s) 43, 50, 98, 111, 188, 232, 402, 420
SfaNI GCATC 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 207
SnaBI TACGTA 1 cut(s) 418
Sse9I AATT 2 cut(s) 194, 327
SsiI CCGC 1 cut(s) 148
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 2 cut(s) 98, 420
TaqI TCGA 3 cut(s) 17, 226, 308
TasI AATT 2 cut(s) 194, 327
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 2 cut(s) 14, 119
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 2 cut(s) 109, 243
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 3 cut(s) 183, 212, 215
Tsp45I GTSAC 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 109, 243
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.