FvH4_4g21960

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008698
Physical Location & Seq
Forward (+)
24914721 .. 24917075
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21960.t1

Sequence Viewer

Length: 948 bp
ATGGATAATTCCACAAATAGGCATACTGATCATTTGGGGGTGAATAAGATGGGGAAAAATATAAGGAAGAGTCCTTTGCATCAACCCAATTTCGGTAATGGTACTGCTGGCGCTGCTAATGCTGTGAATGCTGCTGCTAGGCAGCAGCCTCAGCCTCAGGTGTACAACATAAGCAAGAATGACTTTAGGAACATTGTTCAGCAGCTTACTGGTTCCCCATCACAAGATCCTTTGCCAAGGCCTCCGCAGGGTCCGCCCAAACCCCAAAGTATGCGGCTGCAGAAGATTCGGCCTCCTCCATTGACACCTATCAACAGACCTGTAGTCCCATCTCCTGCCCCTGTACAGGCAGCCCCACCACCAGTTCCTTACAACAATGGCTTTGTTAGGCCTCCCCATTTCGGACAACCATCTCCAACACTGATGCCACCATTTCCTCCTGGAGAAATGTGGCCAAATACCGCTGAGTCTCCAATCTCAGCATATATGCGGTACCTTCAAACTTCAATGCTAGATCCAACTCCAAGGGGAAACCAGCCTCAGCATCAACCACAAGGTCCAGGTCAAATTCAAGGGCAGCCACCATCAACTGGTCTACTTCCCCACCCATCCATGCCTGCGCAGCCGCCTCCAAGAATGAATGGCCCTATACCACCTGCTCCTAATCTCCCATCCCCGCAAATGAATGGTCCTCCACTTTTACCCTCTCCTACTTCCCAGTTCCTATTGCCATCCCCTACCAGTTACAACTACATGTTATCTCCACGGTCACCTTATCCATTGCTTTCACCTGGGACACAGTTTCCTCCACTGACCCCCAATTTTCAGTTTTCACAATCAGGGATTTTAGGTCCAGGGCCTCAAGGCCCACCTTCTCCAGGATTTTTGTATCCTTTATCTCCTTCCGGATTCTTTCCCATTTCAAGTCCGCGATGGAGGGGTCAATAG

Protein Analysis

316

Amino Acids

33.97

Weight (kDa)

10.8

Isoelectric Point (pI)

85.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 50 - 75 3.4e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 664
Acc16I TGCGCA 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 664
Acc65I GGTACC 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 492
AccB7I CCANNNNNTGG 1 cut(s) 590
AccI GTMKAC 1 cut(s) 595
AccII CGCG 1 cut(s) 931
AccIII TCCGGA 1 cut(s) 905
AciI CCGC 8 cut(s) 245, 254, 274, 462, 490, 626, 677, 929
AclWI GGATC 2 cut(s) 221, 509
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 1 cut(s) 567
AfaI GTAC 4 cut(s) 103, 164, 345, 494
AfiI CCNNNNNNNGG 7 cut(s) 18, 92, 248, 346, 401, 590, 878
AflIII ACRYGT 1 cut(s) 753
AgsI TTSAA 4 cut(s) 500, 507, 572, 924
AhdI GACNNNNNGTC 1 cut(s) 323
AjnI CCWGG 5 cut(s) 439, 559, 790, 853, 877
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
Alw26I GTCTC 1 cut(s) 474
AlwI GGATC 2 cut(s) 221, 509
Aor13HI TCCGGA 1 cut(s) 905
AoxI GGCC 7 cut(s) 239, 290, 389, 452, 643, 857, 865
ApoI RAATTY 1 cut(s) 567
ArsI GACNNNNNNTTYG 2 cut(s) 673, 705
Asp718I GGTACC 1 cut(s) 492
AspLEI GCGC 2 cut(s) 113, 622
AspS9I GGNCC 7 cut(s) 251, 557, 644, 689, 851, 857, 866
AsuHPI GGTGA 3 cut(s) 52, 762, 780
AvaII GGWCC 4 cut(s) 251, 557, 689, 851
AxyI CCTNAGG 1 cut(s) 156
BalI TGGCCA 1 cut(s) 454
BanI GGYRCC 1 cut(s) 492
BbvCI CCTCAGC 2 cut(s) 150, 540
BccI CCATC 9 cut(s) 43, 226, 337, 418, 592, 616, 679, 739, 927
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 5 cut(s) 441, 561, 792, 855, 879
BciVI GTATCC 1 cut(s) 900
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 474
BfaI CTAG 2 cut(s) 138, 512
BfmI CTRYAG 2 cut(s) 278, 321
BfoI RGCGCY 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 664
BfuI GTATCC 1 cut(s) 900
Bme1390I CCNGG 5 cut(s) 441, 561, 792, 855, 879
Bme18I GGWCC 4 cut(s) 251, 557, 689, 851
BmeRI GACNNNNNGTC 1 cut(s) 323
BmgT120I GGNCC 7 cut(s) 251, 557, 644, 689, 851, 857, 866
BmiI GGNNCC 3 cut(s) 214, 252, 494
BmrFI CCNGG 5 cut(s) 441, 561, 792, 855, 879
BmrI ACTGGG 1 cut(s) 712
BmsI GCATC 3 cut(s) 88, 414, 553
BmuI ACTGGG 1 cut(s) 712
BpmI CTGGAG 2 cut(s) 462, 861
Bpu10I CCTNAGC 2 cut(s) 150, 540
BpuEI CTTGAG 1 cut(s) 846
BsaJI CCNNGG 5 cut(s) 236, 524, 764, 791, 854
BsaWI WCCGGW 1 cut(s) 905
Bsc4I CCNNNNNNNGG 7 cut(s) 18, 92, 248, 346, 401, 590, 878
Bse1I ACTGG 5 cut(s) 214, 362, 595, 718, 741
Bse21I CCTNAGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 779
BseAI TCCGGA 1 cut(s) 905
BseBI CCWGG 5 cut(s) 441, 561, 792, 855, 879
BseDI CCNNGG 5 cut(s) 236, 524, 764, 791, 854
BseGI GGATG 3 cut(s) 608, 671, 731
BseLI CCNNNNNNNGG 7 cut(s) 18, 92, 248, 346, 401, 590, 878
BseMI GCAATG 1 cut(s) 779
BseMII CTCAG 5 cut(s) 164, 170, 456, 492, 554
BseNI ACTGG 5 cut(s) 214, 362, 595, 718, 741
BseRI GAGGAG 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 931
BshFI GGCC 7 cut(s) 241, 292, 391, 454, 645, 859, 867
BshNI GGYRCC 1 cut(s) 492
BsiSI CCGG 1 cut(s) 906
BslFI GGGAC 2 cut(s) 311, 808
BslI CCNNNNNNNGG 7 cut(s) 18, 92, 248, 346, 401, 590, 878
BsmAI GTCTC 1 cut(s) 474
BsmFI GGGAC 2 cut(s) 311, 808
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 7 cut(s) 241, 292, 391, 454, 645, 859, 867
Bsp13I TCCGGA 1 cut(s) 905
Bsp1407I TGTACA 2 cut(s) 162, 343
Bsp143I GATC 3 cut(s) 28, 226, 514
BspACI CCGC 8 cut(s) 245, 254, 274, 462, 490, 626, 677, 929
BspANI GGCC 7 cut(s) 241, 292, 391, 454, 645, 859, 867
BspCNI CTCAG 5 cut(s) 163, 169, 457, 491, 553
BspEI TCCGGA 1 cut(s) 905
BspFNI CGCG 1 cut(s) 931
BspLI GGNNCC 3 cut(s) 214, 252, 494
BspMAI CTGCAG 1 cut(s) 282
BspMI ACCTGC 1 cut(s) 664
BspPI GGATC 2 cut(s) 221, 509
BspT107I GGYRCC 1 cut(s) 492
BsrDI GCAATG 1 cut(s) 779
BsrGI TGTACA 2 cut(s) 162, 343
BsrI ACTGG 5 cut(s) 214, 362, 595, 718, 741
BssECI CCNNGG 5 cut(s) 236, 524, 764, 791, 854
BssMI GATC 3 cut(s) 28, 226, 514
BssT1I CCWWGG 2 cut(s) 236, 524
Bst2UI CCWGG 5 cut(s) 441, 561, 792, 855, 879
Bst4CI ACNGT 2 cut(s) 768, 801
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 2 cut(s) 162, 343
BstC8I GCNNGC 2 cut(s) 109, 618
BstDEI CTNAG 5 cut(s) 150, 156, 465, 478, 540
BstDSI CCRYGG 1 cut(s) 764
BstEII GGTNACC 1 cut(s) 768
BstENI CCTNNNNNAGG 1 cut(s) 876
BstF5I GGATG 3 cut(s) 608, 671, 731
BstFNI CGCG 1 cut(s) 931
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 2 cut(s) 113, 622
BstKTI GATC 3 cut(s) 31, 229, 517
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 3 cut(s) 28, 226, 514
BstMWI GCNNNNNNNGC 6 cut(s) 113, 119, 128, 151, 253, 622
BstNI CCWGG 5 cut(s) 441, 561, 792, 855, 879
BstNSI RCATGY 1 cut(s) 757
BstPI GGTNACC 1 cut(s) 768
BstSCI CCNGG 5 cut(s) 439, 559, 790, 853, 877
BstSFI CTRYAG 2 cut(s) 278, 321
BstUI CGCG 1 cut(s) 931
BstX2I RGATCY 2 cut(s) 226, 514
BstYI RGATCY 2 cut(s) 226, 514
Bsu36I CCTNAGG 1 cut(s) 156
BsuI GTATCC 1 cut(s) 900
BsuRI GGCC 7 cut(s) 241, 292, 391, 454, 645, 859, 867
BtgI CCRYGG 1 cut(s) 764
BtsCI GGATG 3 cut(s) 608, 671, 731
BtsIMutI CAGTG 2 cut(s) 419, 809
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 2 cut(s) 109, 618
CfoI GCGC 2 cut(s) 113, 622
Cfr13I GGNCC 7 cut(s) 251, 557, 644, 689, 851, 857, 866
Csp6I GTAC 4 cut(s) 102, 163, 344, 493
CviAII CATG 2 cut(s) 613, 754
CviQI GTAC 4 cut(s) 102, 163, 344, 493
DdeI CTNAG 5 cut(s) 150, 156, 465, 478, 540
DpnI GATC 3 cut(s) 30, 228, 516
DpnII GATC 3 cut(s) 28, 226, 514
DriI GACNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 236, 524
Eco147I AGGCCT 2 cut(s) 241, 391
Eco47I GGWCC 4 cut(s) 251, 557, 689, 851
Eco81I CCTNAGG 1 cut(s) 156
Eco91I GGTNACC 1 cut(s) 768
EcoNI CCTNNNNNAGG 1 cut(s) 876
EcoO109I RGGNCCY 1 cut(s) 857
EcoO65I GGTNACC 1 cut(s) 768
EcoRII CCWGG 5 cut(s) 439, 559, 790, 853, 877
EcoT14I CCWWGG 2 cut(s) 236, 524
ErhI CCWWGG 2 cut(s) 236, 524
FaeI CATG 2 cut(s) 616, 757
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 2 cut(s) 311, 808
FatI CATG 2 cut(s) 612, 753
FauI CCCGC 1 cut(s) 684
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 1 cut(s) 595
FokI GGATG 3 cut(s) 595, 658, 718
FspBI CTAG 2 cut(s) 138, 512
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 2 cut(s) 112, 621
GsuI CTGGAG 2 cut(s) 462, 861
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 7 cut(s) 241, 292, 391, 454, 645, 859, 867
HapII CCGG 1 cut(s) 906
HhaI GCGC 2 cut(s) 113, 622
Hin1II CATG 2 cut(s) 616, 757
Hin6I GCGC 2 cut(s) 111, 620
HinP1I GCGC 2 cut(s) 111, 620
HinfI GANTC 4 cut(s) 70, 286, 467, 909
HpaII CCGG 1 cut(s) 906
HphI GGTGA 3 cut(s) 52, 762, 780
Hpy166II GTNNAC 2 cut(s) 163, 596
Hpy188I TCNGA 1 cut(s) 404
Hpy188III TCNNGA 1 cut(s) 906
Hpy8I GTNNAC 2 cut(s) 163, 596
HpyAV CCTTC 3 cut(s) 506, 882, 912
HpyCH4III ACNGT 2 cut(s) 768, 801
HpyCH4V TGCA 2 cut(s) 79, 280
HpyF10VI GCNNNNNNNGC 6 cut(s) 113, 119, 128, 151, 253, 622
HpyF3I CTNAG 5 cut(s) 150, 156, 465, 478, 540
Hsp92II CATG 2 cut(s) 616, 757
HspAI GCGC 2 cut(s) 111, 620
Kpn2I TCCGGA 1 cut(s) 905
KpnI GGTACC 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 28, 226, 514
LmnI GCTCC 1 cut(s) 664
LweI GCATC 3 cut(s) 88, 414, 553
MaeI CTAG 2 cut(s) 138, 512
MaeIII GTNAC 2 cut(s) 743, 768
MalI GATC 3 cut(s) 30, 228, 516
MboI GATC 3 cut(s) 28, 226, 514
MboII GAAGA 2 cut(s) 79, 295
MflI RGATCY 2 cut(s) 226, 514
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 4 cut(s) 7, 88, 567, 820
MluNI TGGCCA 1 cut(s) 454
MlyI GAGTC 2 cut(s) 79, 476
MmeI TCCRAC 2 cut(s) 440, 542
Mox20I TGGCCA 1 cut(s) 454
MroI TCCGGA 1 cut(s) 905
MscI TGGCCA 1 cut(s) 454
Msp20I TGGCCA 1 cut(s) 454
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 5 cut(s) 441, 561, 792, 855, 879
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 5 cut(s) 441, 561, 792, 855, 879
MvnI CGCG 1 cut(s) 931
MwoI GCNNNNNNNGC 6 cut(s) 113, 119, 128, 151, 253, 622
NdeII GATC 3 cut(s) 28, 226, 514
NlaIII CATG 2 cut(s) 616, 757
NlaIV GGNNCC 3 cut(s) 214, 252, 494
NmuCI GTSAC 1 cut(s) 768
NsbI TGCGCA 1 cut(s) 621
NspI RCATGY 1 cut(s) 757
PaqCI CACCTGC 1 cut(s) 664
PceI AGGCCT 2 cut(s) 241, 391
PciI ACATGT 1 cut(s) 753
PctI GAATGC 1 cut(s) 133
PfeI GAWTC 2 cut(s) 286, 909
PflMI CCANNNNNTGG 1 cut(s) 590
PfoI TCCNGGA 2 cut(s) 439, 877
PleI GAGTC 2 cut(s) 78, 475
PpsI GAGTC 2 cut(s) 78, 475
PscI ACATGT 1 cut(s) 753
Psp6I CCWGG 5 cut(s) 439, 559, 790, 853, 877
PspEI GGTNACC 1 cut(s) 768
PspGI CCWGG 5 cut(s) 439, 559, 790, 853, 877
PspN4I GGNNCC 3 cut(s) 214, 252, 494
PspPI GGNCC 7 cut(s) 251, 557, 644, 689, 851, 857, 866
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 2 cut(s) 226, 514
RsaI GTAC 4 cut(s) 103, 164, 345, 494
RsaNI GTAC 4 cut(s) 102, 163, 344, 493
Sau3AI GATC 3 cut(s) 28, 226, 514
Sau96I GGNCC 7 cut(s) 251, 557, 644, 689, 851, 857, 866
SchI GAGTC 2 cut(s) 79, 476
ScrFI CCNGG 5 cut(s) 441, 561, 792, 855, 879
SfaNI GCATC 3 cut(s) 88, 414, 553
SfcI CTRYAG 2 cut(s) 278, 321
SinI GGWCC 4 cut(s) 251, 557, 689, 851
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 4 cut(s) 7, 88, 567, 820
SseBI AGGCCT 2 cut(s) 241, 391
SsiI CCGC 8 cut(s) 245, 254, 274, 462, 490, 626, 677, 929
SspMI CTAG 2 cut(s) 138, 512
StuI AGGCCT 2 cut(s) 241, 391
StyD4I CCNGG 5 cut(s) 439, 559, 790, 853, 877
StyI CCWWGG 2 cut(s) 236, 524
TaaI ACNGT 2 cut(s) 768, 801
TasI AATT 4 cut(s) 7, 88, 567, 820
TatI WGTACW 2 cut(s) 162, 343
TauI GCSGC 2 cut(s) 277, 628
TfiI GAWTC 2 cut(s) 286, 909
TscAI CASTG 2 cut(s) 426, 816
TseFI GTSAC 1 cut(s) 768
Tsp45I GTSAC 1 cut(s) 768
TspDTI ATGAA 2 cut(s) 653, 698
TspRI CASTG 2 cut(s) 426, 816
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 4 cut(s) 251, 557, 689, 851
XagI CCTNNNNNAGG 1 cut(s) 876
XapI RAATTY 1 cut(s) 567
XceI RCATGY 1 cut(s) 757
XmiI GTMKAC 1 cut(s) 595
XspI CTAG 2 cut(s) 138, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.