MD06G1101200.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008698
Physical Location & Seq
Forward (+)
23776096 .. 23779440
3345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1101200.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGATAACTCTGCAAACATGCATAATGATCATTTGGGTGTGAACAAAATGGGGAAAAATATAAGGAAGAGTCCCTTGCACCAACCCAAATTTGGTAATAATGACGCAAGGCAACAACCTCAGCCTCAGGTATACAACATAAGCAAGAATGATTTCAGGAACATAGTTCAGAAGCTTACTGGTTCTCCATCACAGGAACCTTTGCCTAGACCTCCCCAGAATCCGCCAAAGCCCCAAAGTATGCGGTTGCAGAGAATTCGACCTCCCCCGTTAACGCCTATCAACAATAGACCCTTAAACCCACCTCCAGCTCCTCTTCCTGCAGGCCAACCACTGGTTCCCTACAATAATAACTTTGTTAGGCCTCCTCAGGTTGGACATCCATCACCTACACCAATGCCGCCATTTCCACCTGGAGATTCAATGTGGCAAAATACAGCTGAGTCTCCAATCTCAGCATATATGCGGTACCTTCAAAGTTCTATGTTAGATCCAACTCCAAGGGGAAACCAACCTCAGCCTCAACCACAAGTTTCAGATCAGATGCAGTCTCAGCCACCATCAACCGGTTTACTTCCTAACCCATCCATATCTGCTCACCCACCCCCGAGAATGAACGGCCCTGGGCCACATGCAACTAATCTACAGCACCCACAGATGATGGGTCCTCCCCTCTTACCCTCACCAACTTCACAGTTTTTGTTGCCATCCCCAACGGGTTACATGAACTTGTTGTCACCACGGTCACCTTATCCATTGCTTTCACCTGGGATACAGTTTCCTCCACCATGGACCCCCAATTTTCAGTTTTCTCCCATGGGCCAATCAGGGCTATTAGGTCCAGGGCCTCAACCCCCACCTTCTCCCGGCATTTTGTTTCCAATATCTCCTTCGGGGTTTTTCCCCATTTCAAGTCCAAGATGGAGGGGTCATTAA

Protein Analysis

312

Amino Acids

34.08

Weight (kDa)

10.78

Isoelectric Point (pI)

87.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 40 - 65 4.4e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 468
AccB7I CCANNNNNTGG 1 cut(s) 334
AccI GTMKAC 1 cut(s) 132
AciI CCGC 4 cut(s) 224, 244, 401, 466
AclWI GGATC 1 cut(s) 485
AcsI RAATTY 2 cut(s) 89, 255
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
AgeI ACCGGT 1 cut(s) 566
AgsI TTSAA 3 cut(s) 423, 476, 912
AjnI CCWGG 4 cut(s) 412, 622, 766, 841
AjuI GAANNNNNNNTTGG 4 cut(s) 321, 353, 874, 906
AluBI AGCT 3 cut(s) 175, 311, 440
AluI AGCT 3 cut(s) 175, 311, 440
Alw26I GTCTC 2 cut(s) 450, 555
AlwI GGATC 1 cut(s) 485
Ama87I CYCGRG 1 cut(s) 607
AoxI GGCC 6 cut(s) 325, 362, 619, 626, 820, 845
ApoI RAATTY 2 cut(s) 89, 255
AsiGI ACCGGT 1 cut(s) 566
Asp700I GAANNNNTTC 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 468
AspS9I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 6 cut(s) 378, 590, 675, 729, 738, 756
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 3 cut(s) 665, 792, 839
AxyI CCTNAGG 2 cut(s) 126, 369
BanI GGYRCC 1 cut(s) 468
BbvCI CCTCAGC 2 cut(s) 120, 516
BccI CCATC 7 cut(s) 196, 391, 568, 592, 655, 715, 915
BceAI ACGGC 1 cut(s) 634
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 4 cut(s) 414, 624, 768, 843
BciVI GTATCC 1 cut(s) 765
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 2 cut(s) 450, 555
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 2 cut(s) 321, 644
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
Bme1390I CCNGG 5 cut(s) 414, 624, 768, 843, 867
Bme18I GGWCC 3 cut(s) 665, 792, 839
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
BmiI GGNNCC 5 cut(s) 198, 339, 470, 666, 794
BmrFI CCNGG 5 cut(s) 414, 624, 768, 843, 867
BmsI GCATC 1 cut(s) 534
BpmI CTGGAG 2 cut(s) 291, 435
Bpu10I CCTNAGC 2 cut(s) 120, 516
BpuMI CCSGG 1 cut(s) 867
BsaJI CCNNGG 8 cut(s) 500, 622, 623, 740, 767, 788, 816, 842
BsaWI WCCGGW 1 cut(s) 566
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bsc4I CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 2 cut(s) 184, 339
Bse21I CCTNAGG 2 cut(s) 126, 369
Bse3DI GCAATG 1 cut(s) 755
BseBI CCWGG 4 cut(s) 414, 624, 768, 843
BseDI CCNNGG 8 cut(s) 500, 622, 623, 740, 767, 788, 816, 842
BseGI GGATG 3 cut(s) 379, 584, 707
BseLI CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
BseMI GCAATG 1 cut(s) 755
BseMII CTCAG 7 cut(s) 134, 140, 383, 432, 468, 530, 566
BseNI ACTGG 2 cut(s) 184, 339
BseRI GAGGAG 2 cut(s) 303, 357
BshFI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
BshNI GGYRCC 1 cut(s) 468
BshTI ACCGGT 1 cut(s) 566
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 2 cut(s) 567, 867
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
BsmAI GTCTC 2 cut(s) 450, 555
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
BsoBI CYCGRG 1 cut(s) 607
Bsp143I GATC 3 cut(s) 28, 490, 538
Bsp19I CCATGG 2 cut(s) 788, 816
BspACI CCGC 4 cut(s) 224, 244, 401, 466
BspANI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
BspCNI CTCAG 7 cut(s) 133, 139, 382, 433, 467, 529, 565
BspLI GGNNCC 5 cut(s) 198, 339, 470, 666, 794
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 755
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 2 cut(s) 184, 339
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 8 cut(s) 500, 622, 623, 740, 767, 788, 816, 842
BssMI GATC 3 cut(s) 28, 490, 538
BssNAI GTATAC 1 cut(s) 133
BssT1I CCWWGG 3 cut(s) 500, 788, 816
Bst1107I GTATAC 1 cut(s) 133
Bst2UI CCWGG 4 cut(s) 414, 624, 768, 843
Bst4CI ACNGT 3 cut(s) 696, 744, 777
Bst6I CTCTTC 2 cut(s) 62, 321
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 7 cut(s) 120, 126, 369, 441, 454, 516, 552
BstDSI CCRYGG 3 cut(s) 740, 788, 816
BstEII GGTNACC 1 cut(s) 744
BstF5I GGATG 3 cut(s) 379, 584, 707
BstKTI GATC 3 cut(s) 31, 493, 541
BstMAI GTCTC 2 cut(s) 450, 555
BstMBI GATC 3 cut(s) 28, 490, 538
BstMWI GCNNNNNNNGC 1 cut(s) 553
BstNI CCWGG 4 cut(s) 414, 624, 768, 843
BstNSI RCATGY 2 cut(s) 22, 635
BstPI GGTNACC 1 cut(s) 744
BstSCI CCNGG 5 cut(s) 412, 622, 766, 841, 865
BstSFI CTRYAG 2 cut(s) 321, 644
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BstZ17I GTATAC 1 cut(s) 133
Bsu36I CCTNAGG 2 cut(s) 126, 369
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
BtgI CCRYGG 3 cut(s) 740, 788, 816
BtsCI GGATG 3 cut(s) 379, 584, 707
BtsIMutI CAGTG 1 cut(s) 332
Cac8I GCNNGC 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 566
Cfr13I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
CseI GACGC 1 cut(s) 113
Csp6I GTAC 1 cut(s) 469
CspAI ACCGGT 1 cut(s) 566
CviAII CATG 5 cut(s) 19, 632, 724, 789, 817
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 7 cut(s) 120, 126, 369, 441, 454, 516, 552
DpnI GATC 3 cut(s) 30, 492, 540
DpnII GATC 3 cut(s) 28, 490, 538
Eam1104I CTCTTC 2 cut(s) 62, 321
EarI CTCTTC 2 cut(s) 62, 321
EciI GGCGGA 1 cut(s) 213
Eco130I CCWWGG 3 cut(s) 500, 788, 816
Eco147I AGGCCT 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 665, 792, 839
Eco81I CCTNAGG 2 cut(s) 126, 369
Eco88I CYCGRG 1 cut(s) 607
Eco91I GGTNACC 1 cut(s) 744
EcoO109I RGGNCCY 2 cut(s) 665, 845
EcoO65I GGTNACC 1 cut(s) 744
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 4 cut(s) 412, 622, 766, 841
EcoT14I CCWWGG 3 cut(s) 500, 788, 816
EcoT22I ATGCAT 1 cut(s) 24
ErhI CCWWGG 3 cut(s) 500, 788, 816
FaeI CATG 5 cut(s) 22, 635, 727, 792, 820
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 1 cut(s) 57
FatI CATG 5 cut(s) 18, 631, 723, 788, 816
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 3 cut(s) 366, 571, 694
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 1 cut(s) 207
GluI GCNGC 1 cut(s) 401
GsuI CTGGAG 2 cut(s) 291, 435
HaeIII GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
HapII CCGG 2 cut(s) 567, 867
HgaI GACGC 1 cut(s) 113
Hin1II CATG 5 cut(s) 22, 635, 727, 792, 820
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 4 cut(s) 70, 220, 419, 443
HpaI GTTAAC 1 cut(s) 273
HpaII CCGG 2 cut(s) 567, 867
HphI GGTGA 6 cut(s) 378, 590, 675, 729, 738, 756
Hpy166II GTNNAC 4 cut(s) 43, 133, 273, 572
Hpy188I TCNGA 3 cut(s) 171, 538, 543
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 4 cut(s) 43, 133, 273, 572
HpyAV CCTTC 3 cut(s) 482, 870, 900
HpyCH4III ACNGT 3 cut(s) 696, 744, 777
HpyCH4V TGCA 7 cut(s) 14, 22, 79, 250, 323, 547, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 553
HpyF3I CTNAG 7 cut(s) 120, 126, 369, 441, 454, 516, 552
Hsp92II CATG 5 cut(s) 22, 635, 727, 792, 820
KpnI GGTACC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 28
KspAI GTTAAC 1 cut(s) 273
Kzo9I GATC 3 cut(s) 28, 490, 538
LmnI GCTCC 1 cut(s) 316
LweI GCATC 1 cut(s) 534
MaeI CTAG 1 cut(s) 207
MaeIII GTNAC 3 cut(s) 719, 735, 744
MalI GATC 3 cut(s) 30, 492, 540
MboI GATC 3 cut(s) 28, 490, 538
MboII GAAGA 2 cut(s) 79, 308
MflI RGATCY 1 cut(s) 490
MluCI AATT 3 cut(s) 89, 255, 799
MlyI GAGTC 2 cut(s) 79, 452
MmeI TCCRAC 2 cut(s) 355, 518
Mph1103I ATGCAT 1 cut(s) 24
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 3 cut(s) 272, 296, 934
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 2 cut(s) 567, 867
MspR9I CCNGG 5 cut(s) 414, 624, 768, 843, 867
MvaI CCWGG 4 cut(s) 414, 624, 768, 843
MwoI GCNNNNNNNGC 1 cut(s) 553
NciI CCSGG 1 cut(s) 867
NcoI CCATGG 2 cut(s) 788, 816
NdeII GATC 3 cut(s) 28, 490, 538
NlaIII CATG 5 cut(s) 22, 635, 727, 792, 820
NlaIV GGNNCC 5 cut(s) 198, 339, 470, 666, 794
NmuCI GTSAC 2 cut(s) 735, 744
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 2 cut(s) 22, 635
PasI CCCWGGG 1 cut(s) 623
PceI AGGCCT 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 220, 419
PflMI CCANNNNNTGG 1 cut(s) 334
PinAI ACCGGT 1 cut(s) 566
PkrI GCNGC 1 cut(s) 402
PleI GAGTC 2 cut(s) 78, 451
PpsI GAGTC 2 cut(s) 78, 451
PpuMI RGGWCCY 1 cut(s) 665
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 4 cut(s) 412, 622, 766, 841
PspEI GGTNACC 1 cut(s) 744
PspGI CCWGG 4 cut(s) 412, 622, 766, 841
PspN4I GGNNCC 5 cut(s) 198, 339, 470, 666, 794
PspPI GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
PspPPI RGGWCCY 1 cut(s) 665
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 3 cut(s) 272, 296, 934
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 3 cut(s) 28, 490, 538
Sau96I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
SbfI CCTGCAGG 1 cut(s) 325
SchI GAGTC 2 cut(s) 79, 452
ScrFI CCNGG 5 cut(s) 414, 624, 768, 843, 867
SdaI CCTGCAGG 1 cut(s) 325
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 2 cut(s) 321, 644
SinI GGWCC 3 cut(s) 665, 792, 839
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse8387I CCTGCAGG 1 cut(s) 325
Sse9I AATT 3 cut(s) 89, 255, 799
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 4 cut(s) 224, 244, 401, 466
SspMI CTAG 1 cut(s) 207
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 5 cut(s) 412, 622, 766, 841, 865
StyI CCWWGG 3 cut(s) 500, 788, 816
TaaI ACNGT 3 cut(s) 696, 744, 777
TaqI TCGA 1 cut(s) 259
TasI AATT 3 cut(s) 89, 255, 799
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 2 cut(s) 220, 419
Tru1I TTAA 3 cut(s) 272, 296, 934
Tru9I TTAA 3 cut(s) 272, 296, 934
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 2 cut(s) 735, 744
Tsp45I GTSAC 2 cut(s) 735, 744
TspDTI ATGAA 2 cut(s) 629, 740
TspRI CASTG 1 cut(s) 339
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 3 cut(s) 665, 792, 839
XapI RAATTY 2 cut(s) 89, 255
XceI RCATGY 2 cut(s) 22, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.