pycom06g09640

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008698
Physical Location & Seq
Forward (+)
14940212 .. 14941147
936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g09640.1

Sequence Viewer

Length: 936 bp
ATGGATAACTCTGCAAACATGCATAATGATCATTTGGGTGTGAACAAAATGGGGAAAAATATAAGGAAGAGTCCTTTGCACCAACCCAAATTTGGTAATAATGACGCAAGGCAACAACCTCAGCCTCAGGTATACAACATAAGCAAGAATGATTTCAGGAACATTGTTCAGCAGCTTACTGGTTCGCCATCACAGGAACCTTTGCCTAGACCTCCCCAGAATCCACCAAAGCCCCAAAGTATGCGGTTGCAGAGAATTCGACCTCCCCCATTAACGCCTATCAACAATAGACCCTTAAACTCACCTCCAGCTCCTCTTCCTGCAGGCCAACCACAGGTTCCCTACAATAATAACTTTGTTAGGCCTCCTCAGGTTGGACATCCATCACCGACACCAATGCAGCCATTTCCACCTGGAGATTCAATGTGGCAAAATACAGCTGAGTCTCCTATCTCAGCATATATGCGGTACCTTCAAAGTTCGATGTTAGATCTAACTCCAAGGGGAAACCAACCTCAGCCTCAACCACAAGTTTCAGGTCAGATGCAGTCTCAGCCACCATCAACCGGTTTACTTCCTAACCCATCCATATCTGCTCACCCACCCCCAAGAATGAACGGCCCTGGGCCACATGCAACTAATCTACAGCACCCACAGATGAATGGTCCTCCCCTCTTACCGTCACCAACTTCACAGTTTTTATTGCCATCCCCAACGGGTTACATGAACTTGTTGTCTCCACGGTCACCTTATCCACTGCTTTCACCTGGGATACAGTTTCCTCCACCATGGACCCCCAATTTTCAGTTTTCTCCCATGGGCCAATCAGGGCTATTAGGTCCAGGGCCTCAACCCCCACCTTCTCCCGGCATTTTGTTTCCATTATCTCCTTCGGGGTTTTTCCCCATTTCAAGTCCAAGATGGAGGGGTCATTAA

Protein Analysis

312

Amino Acids

34.06

Weight (kDa)

10.95

Isoelectric Point (pI)

87.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 40 - 65 3.7e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 468
AccI GTMKAC 1 cut(s) 132
AciI CCGC 2 cut(s) 244, 466
AcsI RAATTY 2 cut(s) 89, 255
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
AgeI ACCGGT 1 cut(s) 566
AgsI TTSAA 3 cut(s) 423, 476, 912
AjnI CCWGG 4 cut(s) 412, 622, 766, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 3 cut(s) 175, 311, 440
AluI AGCT 3 cut(s) 175, 311, 440
Alw26I GTCTC 3 cut(s) 450, 555, 741
AoxI GGCC 6 cut(s) 325, 362, 619, 626, 820, 845
ApeKI GCWGC 2 cut(s) 172, 400
ApoI RAATTY 2 cut(s) 89, 255
AsiGI ACCGGT 1 cut(s) 566
Asp700I GAANNNNTTC 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 468
AspS9I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 6 cut(s) 294, 378, 590, 675, 738, 756
AvaII GGWCC 3 cut(s) 665, 792, 839
AxyI CCTNAGG 2 cut(s) 126, 369
BanI GGYRCC 1 cut(s) 468
BbvCI CCTCAGC 2 cut(s) 120, 516
BbvI GCAGC 2 cut(s) 184, 412
BccI CCATC 6 cut(s) 196, 391, 568, 592, 715, 915
BceAI ACGGC 1 cut(s) 634
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 379, 413
BciT130I CCWGG 4 cut(s) 414, 624, 768, 843
BciVI GTATCC 1 cut(s) 765
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 3 cut(s) 450, 555, 741
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 2 cut(s) 321, 644
BfuI GTATCC 1 cut(s) 765
BglII AGATCT 1 cut(s) 490
BisI GCNGC 2 cut(s) 173, 401
BlsI GCNGC 2 cut(s) 174, 402
Bme1390I CCNGG 5 cut(s) 414, 624, 768, 843, 867
Bme18I GGWCC 3 cut(s) 665, 792, 839
BmgT120I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
BmiI GGNNCC 4 cut(s) 198, 339, 470, 794
BmrFI CCNGG 5 cut(s) 414, 624, 768, 843, 867
BmsI GCATC 1 cut(s) 534
BpmI CTGGAG 2 cut(s) 291, 435
Bpu10I CCTNAGC 2 cut(s) 120, 516
BpuMI CCSGG 1 cut(s) 867
BsaJI CCNNGG 8 cut(s) 500, 622, 623, 740, 767, 788, 816, 842
BsaWI WCCGGW 1 cut(s) 566
Bsc4I CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 1 cut(s) 184
Bse21I CCTNAGG 2 cut(s) 126, 369
BseBI CCWGG 4 cut(s) 414, 624, 768, 843
BseDI CCNNGG 8 cut(s) 500, 622, 623, 740, 767, 788, 816, 842
BseGI GGATG 3 cut(s) 379, 584, 707
BseLI CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
BseMII CTCAG 7 cut(s) 134, 140, 383, 432, 468, 530, 566
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 2 cut(s) 303, 357
BseXI GCAGC 2 cut(s) 184, 412
BshFI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
BshNI GGYRCC 1 cut(s) 468
BshTI ACCGGT 1 cut(s) 566
BsiSI CCGG 2 cut(s) 567, 867
BslI CCNNNNNNNGG 5 cut(s) 92, 334, 374, 566, 866
BsmAI GTCTC 3 cut(s) 450, 555, 741
BsnI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
Bsp143I GATC 2 cut(s) 28, 490
Bsp19I CCATGG 2 cut(s) 788, 816
BspACI CCGC 2 cut(s) 244, 466
BspANI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
BspCNI CTCAG 7 cut(s) 133, 139, 382, 433, 467, 529, 565
BspLI GGNNCC 4 cut(s) 198, 339, 470, 794
BspMAI CTGCAG 1 cut(s) 325
BspT107I GGYRCC 1 cut(s) 468
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 8 cut(s) 500, 622, 623, 740, 767, 788, 816, 842
BssMI GATC 2 cut(s) 28, 490
BssNAI GTATAC 1 cut(s) 133
BssT1I CCWWGG 3 cut(s) 500, 788, 816
Bst1107I GTATAC 1 cut(s) 133
Bst2UI CCWGG 4 cut(s) 414, 624, 768, 843
Bst4CI ACNGT 4 cut(s) 681, 696, 744, 777
Bst6I CTCTTC 2 cut(s) 62, 321
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 7 cut(s) 120, 126, 369, 441, 454, 516, 552
BstDSI CCRYGG 3 cut(s) 740, 788, 816
BstEII GGTNACC 1 cut(s) 744
BstF5I GGATG 3 cut(s) 379, 584, 707
BstKTI GATC 2 cut(s) 31, 493
BstMAI GTCTC 3 cut(s) 450, 555, 741
BstMBI GATC 2 cut(s) 28, 490
BstMWI GCNNNNNNNGC 1 cut(s) 553
BstNI CCWGG 4 cut(s) 414, 624, 768, 843
BstNSI RCATGY 2 cut(s) 22, 635
BstPI GGTNACC 1 cut(s) 744
BstSCI CCNGG 5 cut(s) 412, 622, 766, 841, 865
BstSFI CTRYAG 2 cut(s) 321, 644
BstV1I GCAGC 2 cut(s) 184, 412
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BstZ17I GTATAC 1 cut(s) 133
Bsu36I CCTNAGG 2 cut(s) 126, 369
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
BtgI CCRYGG 3 cut(s) 740, 788, 816
BtsCI GGATG 3 cut(s) 379, 584, 707
BtsI GCAGTG 1 cut(s) 755
BtsIMutI CAGTG 1 cut(s) 755
Cac8I GCNNGC 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 566
Cfr13I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
CseI GACGC 1 cut(s) 113
Csp6I GTAC 1 cut(s) 469
CspAI ACCGGT 1 cut(s) 566
CviAII CATG 5 cut(s) 19, 632, 724, 789, 817
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 7 cut(s) 120, 126, 369, 441, 454, 516, 552
DpnI GATC 2 cut(s) 30, 492
DpnII GATC 2 cut(s) 28, 490
Eam1104I CTCTTC 2 cut(s) 62, 321
EarI CTCTTC 2 cut(s) 62, 321
Eco130I CCWWGG 3 cut(s) 500, 788, 816
Eco147I AGGCCT 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 665, 792, 839
Eco81I CCTNAGG 2 cut(s) 126, 369
Eco91I GGTNACC 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 845
EcoO65I GGTNACC 1 cut(s) 744
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 4 cut(s) 412, 622, 766, 841
EcoT14I CCWWGG 3 cut(s) 500, 788, 816
EcoT22I ATGCAT 1 cut(s) 24
ErhI CCWWGG 3 cut(s) 500, 788, 816
FaeI CATG 5 cut(s) 22, 635, 727, 792, 820
FatI CATG 5 cut(s) 18, 631, 723, 788, 816
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 173, 401
FokI GGATG 3 cut(s) 366, 571, 694
Fsp4HI GCNGC 2 cut(s) 173, 401
FspBI CTAG 1 cut(s) 207
GluI GCNGC 2 cut(s) 173, 401
GsuI CTGGAG 2 cut(s) 291, 435
HaeIII GGCC 6 cut(s) 327, 364, 621, 628, 822, 847
HapII CCGG 2 cut(s) 567, 867
HgaI GACGC 1 cut(s) 113
Hin1II CATG 5 cut(s) 22, 635, 727, 792, 820
HinfI GANTC 4 cut(s) 70, 220, 419, 443
HpaII CCGG 2 cut(s) 567, 867
HphI GGTGA 6 cut(s) 294, 378, 590, 675, 738, 756
Hpy166II GTNNAC 3 cut(s) 43, 133, 572
Hpy188I TCNGA 1 cut(s) 543
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 3 cut(s) 43, 133, 572
HpyAV CCTTC 3 cut(s) 482, 870, 900
HpyCH4III ACNGT 4 cut(s) 681, 696, 744, 777
HpyCH4V TGCA 8 cut(s) 14, 22, 79, 250, 323, 400, 547, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 553
HpyF3I CTNAG 7 cut(s) 120, 126, 369, 441, 454, 516, 552
Hsp92II CATG 5 cut(s) 22, 635, 727, 792, 820
KpnI GGTACC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 2 cut(s) 28, 490
LmnI GCTCC 1 cut(s) 316
Lsp1109I GCAGC 2 cut(s) 184, 412
LweI GCATC 1 cut(s) 534
MaeI CTAG 1 cut(s) 207
MaeIII GTNAC 3 cut(s) 681, 719, 744
MalI GATC 2 cut(s) 30, 492
MboI GATC 2 cut(s) 28, 490
MboII GAAGA 2 cut(s) 79, 308
MflI RGATCY 1 cut(s) 490
MluCI AATT 3 cut(s) 89, 255, 799
MlyI GAGTC 2 cut(s) 79, 452
MmeI TCCRAC 1 cut(s) 355
Mph1103I ATGCAT 1 cut(s) 24
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 3 cut(s) 272, 296, 934
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 2 cut(s) 567, 867
MspR9I CCNGG 5 cut(s) 414, 624, 768, 843, 867
MvaI CCWGG 4 cut(s) 414, 624, 768, 843
MwoI GCNNNNNNNGC 1 cut(s) 553
NciI CCSGG 1 cut(s) 867
NcoI CCATGG 2 cut(s) 788, 816
NdeII GATC 2 cut(s) 28, 490
NlaIII CATG 5 cut(s) 22, 635, 727, 792, 820
NlaIV GGNNCC 4 cut(s) 198, 339, 470, 794
NmuCI GTSAC 2 cut(s) 681, 744
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 2 cut(s) 22, 635
PasI CCCWGGG 1 cut(s) 623
PceI AGGCCT 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 220, 419
PinAI ACCGGT 1 cut(s) 566
PkrI GCNGC 2 cut(s) 174, 402
PleI GAGTC 2 cut(s) 78, 451
PpsI GAGTC 2 cut(s) 78, 451
Psp6I CCWGG 4 cut(s) 412, 622, 766, 841
PspEI GGTNACC 1 cut(s) 744
PspGI CCWGG 4 cut(s) 412, 622, 766, 841
PspN4I GGNNCC 4 cut(s) 198, 339, 470, 794
PspPI GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 3 cut(s) 272, 296, 934
SatI GCNGC 2 cut(s) 173, 401
Sau3AI GATC 2 cut(s) 28, 490
Sau96I GGNCC 7 cut(s) 620, 626, 665, 792, 820, 839, 845
SbfI CCTGCAGG 1 cut(s) 325
SchI GAGTC 2 cut(s) 79, 452
ScrFI CCNGG 5 cut(s) 414, 624, 768, 843, 867
SdaI CCTGCAGG 1 cut(s) 325
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 2 cut(s) 321, 644
SinI GGWCC 3 cut(s) 665, 792, 839
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse8387I CCTGCAGG 1 cut(s) 325
Sse9I AATT 3 cut(s) 89, 255, 799
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 2 cut(s) 244, 466
SspMI CTAG 1 cut(s) 207
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 5 cut(s) 412, 622, 766, 841, 865
StyI CCWWGG 3 cut(s) 500, 788, 816
TaaI ACNGT 4 cut(s) 681, 696, 744, 777
TaqI TCGA 2 cut(s) 259, 482
TasI AATT 3 cut(s) 89, 255, 799
TfiI GAWTC 2 cut(s) 220, 419
Tru1I TTAA 3 cut(s) 272, 296, 934
Tru9I TTAA 3 cut(s) 272, 296, 934
TscAI CASTG 1 cut(s) 762
TseFI GTSAC 2 cut(s) 681, 744
TseI GCWGC 2 cut(s) 172, 400
Tsp45I GTSAC 2 cut(s) 681, 744
TspDTI ATGAA 3 cut(s) 629, 674, 740
TspRI CASTG 1 cut(s) 762
VpaK11BI GGWCC 3 cut(s) 665, 792, 839
XapI RAATTY 2 cut(s) 89, 255
XceI RCATGY 2 cut(s) 22, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.