Rh7AG249300

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008698
Physical Location & Seq
Reverse (-)
24722693 .. 24723637
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG249300.1

Sequence Viewer

Length: 945 bp
ATGGATAACTCCACAAATAGGCATACTGATCATTTGGGGGTGAACAAGATTGGGAAAAATATAAGGAAGAGTCCTTTGCACCAGCCCAATTTCGGTAATGCTGCTGCTAGTGCTGCTAATGCTGTGAATGCTGCTAGGCAACAGCCTCAGCCTCAGGTGTACAACATAAGCAAGAACGACTTTAGGAACATTGTTCAGCAGCTTACTGGTTCGCCATCACAAGATCCTTTGCCGAGGCCTCCCCAGGGTCCGCCCAAACCCCAAAGTATGCGGTTGCAGAAGATCCGACCTCCTCCATTGACACCTATCAACAGACCTGTAGTCCCACCTCCAGTCCCTGTACAGGCAGCCCCGCCGCCTGTTCCTTACAATAATGGCTTTGTTAGGCCTCCCCATTTTGGACAACCATCACCAACACTGATGCCACCGTTTCCACCTGGAGATTCAATGTGGCCGAATACAGCTGAGTCTCCAATCTCAGCATATATGCGGTACCTTCAAAGTTCAATGATGGATCCAACTCCAAGGGGAAACCAGCCTCAGCCTCAACCACAAGGTCCAGGTCAAATTCAAGGACAGCCACCATCAACTGGTTTACTTCCCCACCCATCCATGCCTGCGCAGCCGCCCCCAAGAATGAATGGCCCTGTACCACCTGCACCTAATCTCCCATCCCCGCAAATGGGTGGTCCTCCACTGTTACCCTCTCCAACTTCCCAGTTCCTATTGCCATCCCCAACCGGTTACAACTACTTGTTATCTCCACGGTCACCTTATCCATTGCTTTCTCCTGGGACACAGTTTCCTCCACTGACCCCCAATTTTCAGTTTTCACAATCAGGGATATTAGGTCCAGGGCCTCAAGCCCCACCTTCTCCAGGCTTTTTATATCCATTATCTCCTTCAGGGTTTTTTCCCATATCAAGTCCACGATGGAGGGGTTAA

Protein Analysis

314

Amino Acids

33.74

Weight (kDa)

10.8

Isoelectric Point (pI)

89.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 49 - 74 3.4e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 664
Acc16I TGCGCA 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 664
Acc65I GGTACC 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 492
AccB7I CCANNNNNTGG 1 cut(s) 590
AciI CCGC 7 cut(s) 251, 271, 353, 356, 490, 626, 677
AclWI GGATC 4 cut(s) 218, 277, 509, 522
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 1 cut(s) 567
AcuI CTGAAG 1 cut(s) 888
AfaI GTAC 4 cut(s) 161, 342, 494, 651
AfiI CCNNNNNNNGG 9 cut(s) 18, 92, 245, 343, 398, 590, 682, 683, 878
AgeI ACCGGT 1 cut(s) 740
AgsI TTSAA 4 cut(s) 447, 500, 507, 572
AhdI GACNNNNNGTC 1 cut(s) 320
AjnI CCWGG 6 cut(s) 243, 436, 559, 790, 853, 877
AluBI AGCT 2 cut(s) 202, 464
AluI AGCT 2 cut(s) 202, 464
Alw26I GTCTC 1 cut(s) 474
AlwI GGATC 4 cut(s) 218, 277, 509, 522
AlwNI CAGNNNCTG 1 cut(s) 338
AoxI GGCC 5 cut(s) 236, 386, 452, 643, 857
ApeKI GCWGC 7 cut(s) 101, 104, 113, 131, 199, 347, 622
ApoI RAATTY 1 cut(s) 567
ArsI GACNNNNNNTTYG 2 cut(s) 673, 705
AsiGI ACCGGT 1 cut(s) 740
Asp718I GGTACC 1 cut(s) 492
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 6 cut(s) 248, 557, 644, 689, 851, 857
AsuHPI GGTGA 3 cut(s) 52, 402, 762
AvaII GGWCC 4 cut(s) 248, 557, 689, 851
AxyI CCTNAGG 1 cut(s) 153
BamHI GGATCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 492
BbvCI CCTCAGC 2 cut(s) 147, 540
BbvI GCAGC 7 cut(s) 88, 91, 100, 118, 211, 359, 634
BccI CCATC 8 cut(s) 223, 415, 505, 592, 616, 679, 739, 927
BciT130I CCWGG 6 cut(s) 245, 438, 561, 792, 855, 879
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 474
BfaI CTAG 2 cut(s) 108, 135
BfmI CTRYAG 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 664
BisI GCNGC 9 cut(s) 102, 105, 114, 132, 200, 348, 356, 623, 626
BlsI GCNGC 9 cut(s) 103, 106, 115, 133, 201, 349, 357, 624, 627
Bme1390I CCNGG 6 cut(s) 245, 438, 561, 792, 855, 879
Bme18I GGWCC 4 cut(s) 248, 557, 689, 851
BmeRI GACNNNNNGTC 1 cut(s) 320
BmgT120I GGNCC 6 cut(s) 248, 557, 644, 689, 851, 857
BmiI GGNNCC 3 cut(s) 249, 494, 516
BmrFI CCNGG 6 cut(s) 245, 438, 561, 792, 855, 879
BmrI ACTGGG 1 cut(s) 712
BmsI GCATC 1 cut(s) 411
BmuI ACTGGG 1 cut(s) 712
BpmI CTGGAG 3 cut(s) 315, 459, 861
Bpu10I CCTNAGC 2 cut(s) 147, 540
BpuEI CTTGAG 1 cut(s) 846
BsaJI CCNNGG 7 cut(s) 233, 243, 244, 524, 764, 791, 854
BsaWI WCCGGW 1 cut(s) 740
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 9 cut(s) 18, 92, 245, 343, 398, 590, 682, 683, 878
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 4 cut(s) 211, 332, 595, 718
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 1 cut(s) 779
BseBI CCWGG 6 cut(s) 245, 438, 561, 792, 855, 879
BseDI CCNNGG 7 cut(s) 233, 243, 244, 524, 764, 791, 854
BseGI GGATG 3 cut(s) 608, 671, 731
BseLI CCNNNNNNNGG 9 cut(s) 18, 92, 245, 343, 398, 590, 682, 683, 878
BseMI GCAATG 1 cut(s) 779
BseMII CTCAG 5 cut(s) 161, 167, 456, 492, 554
BseNI ACTGG 4 cut(s) 211, 332, 595, 718
BseRI GAGGAG 1 cut(s) 282
BseXI GCAGC 7 cut(s) 88, 91, 100, 118, 211, 359, 634
BsgI GTGCAG 1 cut(s) 642
BshFI GGCC 5 cut(s) 238, 388, 454, 645, 859
BshNI GGYRCC 1 cut(s) 492
BshTI ACCGGT 1 cut(s) 740
BsiSI CCGG 1 cut(s) 741
BslFI GGGAC 3 cut(s) 308, 320, 808
BslI CCNNNNNNNGG 9 cut(s) 18, 92, 245, 343, 398, 590, 682, 683, 878
BsmAI GTCTC 1 cut(s) 474
BsmFI GGGAC 3 cut(s) 308, 320, 808
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 5 cut(s) 238, 388, 454, 645, 859
Bsp1407I TGTACA 2 cut(s) 159, 340
Bsp143I GATC 4 cut(s) 28, 223, 282, 514
BspACI CCGC 7 cut(s) 251, 271, 353, 356, 490, 626, 677
BspANI GGCC 5 cut(s) 238, 388, 454, 645, 859
BspCNI CTCAG 5 cut(s) 160, 166, 457, 491, 553
BspLI GGNNCC 3 cut(s) 249, 494, 516
BspMI ACCTGC 1 cut(s) 664
BspPI GGATC 4 cut(s) 218, 277, 509, 522
BspT107I GGYRCC 1 cut(s) 492
BsrDI GCAATG 1 cut(s) 779
BsrFI RCCGGY 1 cut(s) 740
BsrGI TGTACA 2 cut(s) 159, 340
BsrI ACTGG 4 cut(s) 211, 332, 595, 718
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 7 cut(s) 233, 243, 244, 524, 764, 791, 854
BssMI GATC 4 cut(s) 28, 223, 282, 514
BssT1I CCWWGG 1 cut(s) 524
Bst2UI CCWGG 6 cut(s) 245, 438, 561, 792, 855, 879
Bst4CI ACNGT 4 cut(s) 429, 699, 768, 801
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 2 cut(s) 159, 340
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 5 cut(s) 147, 153, 465, 478, 540
BstDSI CCRYGG 1 cut(s) 764
BstEII GGTNACC 1 cut(s) 768
BstENI CCTNNNNNAGG 1 cut(s) 876
BstF5I GGATG 3 cut(s) 608, 671, 731
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 4 cut(s) 31, 226, 285, 517
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 4 cut(s) 28, 223, 282, 514
BstMWI GCNNNNNNNGC 5 cut(s) 110, 113, 119, 128, 622
BstNI CCWGG 6 cut(s) 245, 438, 561, 792, 855, 879
BstPI GGTNACC 1 cut(s) 768
BstSCI CCNGG 6 cut(s) 243, 436, 559, 790, 853, 877
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 7 cut(s) 88, 91, 100, 118, 211, 359, 634
BstX2I RGATCY 3 cut(s) 223, 282, 514
BstYI RGATCY 3 cut(s) 223, 282, 514
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 5 cut(s) 238, 388, 454, 645, 859
BtgI CCRYGG 1 cut(s) 764
BtsCI GGATG 3 cut(s) 608, 671, 731
BtsIMutI CAGTG 3 cut(s) 416, 695, 809
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 1 cut(s) 618
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 6 cut(s) 248, 557, 644, 689, 851, 857
Csp6I GTAC 4 cut(s) 160, 341, 493, 650
CspAI ACCGGT 1 cut(s) 740
CviAII CATG 1 cut(s) 613
CviQI GTAC 4 cut(s) 160, 341, 493, 650
DdeI CTNAG 5 cut(s) 147, 153, 465, 478, 540
DpnI GATC 4 cut(s) 30, 225, 284, 516
DpnII GATC 4 cut(s) 28, 223, 282, 514
DriI GACNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 524
Eco147I AGGCCT 2 cut(s) 238, 388
Eco47I GGWCC 4 cut(s) 248, 557, 689, 851
Eco57I CTGAAG 1 cut(s) 888
Eco81I CCTNAGG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 768
EcoNI CCTNNNNNAGG 1 cut(s) 876
EcoO109I RGGNCCY 1 cut(s) 857
EcoO65I GGTNACC 1 cut(s) 768
EcoRII CCWGG 6 cut(s) 243, 436, 559, 790, 853, 877
EcoT14I CCWWGG 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 524
FaeI CATG 1 cut(s) 616
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FaqI GGGAC 3 cut(s) 308, 320, 808
FatI CATG 1 cut(s) 612
FauI CCCGC 2 cut(s) 360, 684
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 9 cut(s) 102, 105, 114, 132, 200, 348, 356, 623, 626
FokI GGATG 3 cut(s) 595, 658, 718
Fsp4HI GCNGC 9 cut(s) 102, 105, 114, 132, 200, 348, 356, 623, 626
FspBI CTAG 2 cut(s) 108, 135
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 1 cut(s) 621
GluI GCNGC 9 cut(s) 102, 105, 114, 132, 200, 348, 356, 623, 626
GsuI CTGGAG 3 cut(s) 315, 459, 861
HaeIII GGCC 5 cut(s) 238, 388, 454, 645, 859
HapII CCGG 1 cut(s) 741
HhaI GCGC 1 cut(s) 622
Hin1II CATG 1 cut(s) 616
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HinfI GANTC 3 cut(s) 70, 443, 467
HpaII CCGG 1 cut(s) 741
HphI GGTGA 3 cut(s) 52, 402, 762
Hpy166II GTNNAC 4 cut(s) 43, 160, 596, 929
Hpy188I TCNGA 1 cut(s) 287
Hpy8I GTNNAC 4 cut(s) 43, 160, 596, 929
HpyAV CCTTC 3 cut(s) 506, 882, 912
HpyCH4III ACNGT 4 cut(s) 429, 699, 768, 801
HpyCH4V TGCA 3 cut(s) 79, 277, 659
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 113, 119, 128, 622
HpyF3I CTNAG 5 cut(s) 147, 153, 465, 478, 540
Hsp92II CATG 1 cut(s) 616
HspAI GCGC 1 cut(s) 620
KpnI GGTACC 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 4 cut(s) 28, 223, 282, 514
Lsp1109I GCAGC 7 cut(s) 88, 91, 100, 118, 211, 359, 634
LweI GCATC 1 cut(s) 411
MaeI CTAG 2 cut(s) 108, 135
MaeIII GTNAC 3 cut(s) 699, 743, 768
MalI GATC 4 cut(s) 30, 225, 284, 516
MboI GATC 4 cut(s) 28, 223, 282, 514
MboII GAAGA 2 cut(s) 79, 292
MflI RGATCY 3 cut(s) 223, 282, 514
MluCI AATT 3 cut(s) 88, 567, 820
MlyI GAGTC 2 cut(s) 79, 476
MmeI TCCRAC 3 cut(s) 310, 542, 734
MseI TTAA 1 cut(s) 943
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 1 cut(s) 741
MspR9I CCNGG 6 cut(s) 245, 438, 561, 792, 855, 879
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 6 cut(s) 245, 438, 561, 792, 855, 879
MwoI GCNNNNNNNGC 5 cut(s) 110, 113, 119, 128, 622
NdeII GATC 4 cut(s) 28, 223, 282, 514
NlaIII CATG 1 cut(s) 616
NlaIV GGNNCC 3 cut(s) 249, 494, 516
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 768
NsbI TGCGCA 1 cut(s) 621
PaqCI CACCTGC 1 cut(s) 664
PasI CCCWGGG 1 cut(s) 244
PceI AGGCCT 2 cut(s) 238, 388
PctI GAATGC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 443
PflMI CCANNNNNTGG 1 cut(s) 590
PinAI ACCGGT 1 cut(s) 740
PkrI GCNGC 9 cut(s) 103, 106, 115, 133, 201, 349, 357, 624, 627
PleI GAGTC 2 cut(s) 78, 475
PpsI GAGTC 2 cut(s) 78, 475
Psp6I CCWGG 6 cut(s) 243, 436, 559, 790, 853, 877
PspEI GGTNACC 1 cut(s) 768
PspGI CCWGG 6 cut(s) 243, 436, 559, 790, 853, 877
PspN4I GGNNCC 3 cut(s) 249, 494, 516
PspPI GGNCC 6 cut(s) 248, 557, 644, 689, 851, 857
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 3 cut(s) 223, 282, 514
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 4 cut(s) 161, 342, 494, 651
RsaNI GTAC 4 cut(s) 160, 341, 493, 650
SaqAI TTAA 1 cut(s) 943
SatI GCNGC 9 cut(s) 102, 105, 114, 132, 200, 348, 356, 623, 626
Sau3AI GATC 4 cut(s) 28, 223, 282, 514
Sau96I GGNCC 6 cut(s) 248, 557, 644, 689, 851, 857
SchI GAGTC 2 cut(s) 79, 476
ScrFI CCNGG 6 cut(s) 245, 438, 561, 792, 855, 879
SfaNI GCATC 1 cut(s) 411
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 4 cut(s) 248, 557, 689, 851
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 3 cut(s) 88, 567, 820
SseBI AGGCCT 2 cut(s) 238, 388
SsiI CCGC 7 cut(s) 251, 271, 353, 356, 490, 626, 677
SspMI CTAG 2 cut(s) 108, 135
StuI AGGCCT 2 cut(s) 238, 388
StyD4I CCNGG 6 cut(s) 243, 436, 559, 790, 853, 877
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 4 cut(s) 429, 699, 768, 801
TasI AATT 3 cut(s) 88, 567, 820
TatI WGTACW 2 cut(s) 159, 340
TauI GCSGC 2 cut(s) 358, 628
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 1 cut(s) 943
Tru9I TTAA 1 cut(s) 943
TscAI CASTG 3 cut(s) 423, 702, 816
TseFI GTSAC 1 cut(s) 768
TseI GCWGC 7 cut(s) 101, 104, 113, 131, 199, 347, 622
Tsp45I GTSAC 1 cut(s) 768
TspDTI ATGAA 1 cut(s) 653
TspRI CASTG 3 cut(s) 423, 702, 816
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 4 cut(s) 248, 557, 689, 851
XagI CCTNNNNNAGG 1 cut(s) 876
XapI RAATTY 1 cut(s) 567
XspI CTAG 2 cut(s) 108, 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.