MD14G1120900.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008698
Physical Location & Seq
Forward (+)
19545481 .. 19549350
3870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1120900.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGATAACTCTGCAAACATGCATAATGATCATTTGGGTGTGAACAAAATGGGGAAAAATATAAGGAAGAGTCCTTTGCACCAACCCAAATTTGGTAATAATGACGCCAGGCAACAACCTCAACCTCAGGTGTACAATATAAGCAAGAATGACTTCCGGAACATTGTTCAGAAGCTTACTGGTTCGCCATCGCAAGAACCTTTGCCTAGACCTCCCCAGAATCCGCCAAAGCCCCAAAGTATGCGATTGCAGAGAATTCGACCTCCCCCATTAGCACCTATCAACAACAGACCCTTAAACACACCTCCAGCTCCTCTTCCTGCAGGCCCACCACAGGTTCCCTACAATAATAACTTTGTTAGGCCTCCTCAGTTTGGACATCCATCACCTACACCAATGCCACCATTTCCACATGGAGATTCAATGTGGCAAAATACAGCTGAATCTCCTATCTCAGCATATATGCGGTACCTTCAGAGTTCAATGTTAGATCCAACTCCAAGGGGCAACCAACCTCAGCCTCAACCACAAGTTCCAGGTCAAATGCAGTCTCAGCCACCATCAACCAGTTTACTTCCTAACCCATCCGTATCTGCTCACCCACCCCCGAGAATGAATGCCCCTGGGGCACATGCGCCTAATCTACAGCACCCACAGATGAATGGTCCTCCCCTCTTACCGTCACCAACTTCACAGTTTTTATTGCCATCCCCAACGGGTTACATGAACTTGTTGTCTCCACGGTCACCTTATCCATTGCTTTCACCTGGGATGCAGTTTCCTCCACCATGGACCCCCAATTTTCAGTTCTCTCCAATGGGCCAATCAGGGCTGTTAGGTCCAGGGCCTCAACCCCCACCTTCTCCTGGCGTTTTGTTTCCATTATCTCCTTCAGGGTTTTTCCCCATTTCAAGTCCAAGATGGAGGGATCATCACTAG

Protein Analysis

313

Amino Acids

34.26

Weight (kDa)

10.78

Isoelectric Point (pI)

88.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 40 - 65 4.5e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 468
AccIII TCCGGA 1 cut(s) 156
AciI CCGC 2 cut(s) 224, 466
AclWI GGATC 2 cut(s) 485, 936
AcsI RAATTY 2 cut(s) 89, 255
AcuI CTGAAG 2 cut(s) 458, 876
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 2 cut(s) 134, 470
AfiI CCNNNNNNNGG 4 cut(s) 92, 334, 374, 866
AgsI TTSAA 3 cut(s) 423, 483, 912
AjnI CCWGG 6 cut(s) 107, 535, 622, 766, 841, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 791, 823
AluBI AGCT 3 cut(s) 175, 311, 440
AluI AGCT 3 cut(s) 175, 311, 440
Alw26I GTCTC 2 cut(s) 555, 741
AlwI GGATC 2 cut(s) 485, 936
Ama87I CYCGRG 1 cut(s) 607
Aor13HI TCCGGA 1 cut(s) 156
AoxI GGCC 4 cut(s) 325, 362, 820, 845
ApoI RAATTY 2 cut(s) 89, 255
Asp700I GAANNNNTTC 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 468
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 6 cut(s) 326, 665, 792, 820, 839, 845
AsuHPI GGTGA 5 cut(s) 378, 590, 675, 738, 756
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 3 cut(s) 665, 792, 839
AxyI CCTNAGG 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 468
BbvCI CCTCAGC 1 cut(s) 516
BccI CCATC 6 cut(s) 196, 391, 568, 592, 715, 915
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 6 cut(s) 109, 537, 624, 768, 843, 867
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 2 cut(s) 555, 741
BfaI CTAG 2 cut(s) 207, 937
BfmI CTRYAG 2 cut(s) 321, 644
BglI GCCNNNNNGGC 1 cut(s) 626
Bme1390I CCNGG 6 cut(s) 109, 537, 624, 768, 843, 867
Bme18I GGWCC 3 cut(s) 665, 792, 839
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 6 cut(s) 326, 665, 792, 820, 839, 845
BmiI GGNNCC 3 cut(s) 339, 470, 794
BmrFI CCNGG 6 cut(s) 109, 537, 624, 768, 843, 867
BmsI GCATC 1 cut(s) 762
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 7 cut(s) 500, 622, 623, 740, 767, 788, 842
BsaWI WCCGGW 1 cut(s) 156
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 334, 374, 866
Bse1I ACTGG 2 cut(s) 184, 567
Bse21I CCTNAGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 755
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 6 cut(s) 109, 537, 624, 768, 843, 867
BseDI CCNNGG 7 cut(s) 500, 622, 623, 740, 767, 788, 842
BseGI GGATG 4 cut(s) 379, 584, 707, 777
BseLI CCNNNNNNNGG 4 cut(s) 92, 334, 374, 866
BseMI GCAATG 1 cut(s) 755
BseMII CTCAG 5 cut(s) 140, 383, 468, 530, 566
BseNI ACTGG 2 cut(s) 184, 567
BseRI GAGGAG 2 cut(s) 303, 357
BseSI GKGCMC 1 cut(s) 631
BshFI GGCC 4 cut(s) 327, 364, 822, 847
BshNI GGYRCC 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 1 cut(s) 157
BslI CCNNNNNNNGG 4 cut(s) 92, 334, 374, 866
BsmAI GTCTC 2 cut(s) 555, 741
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 4 cut(s) 327, 364, 822, 847
BsoBI CYCGRG 1 cut(s) 607
Bsp1286I GDGCHC 1 cut(s) 631
Bsp13I TCCGGA 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 3 cut(s) 28, 490, 928
Bsp19I CCATGG 1 cut(s) 788
BspACI CCGC 2 cut(s) 224, 466
BspANI GGCC 4 cut(s) 327, 364, 822, 847
BspCNI CTCAG 5 cut(s) 139, 382, 467, 529, 565
BspEI TCCGGA 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 339, 470, 794
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 2 cut(s) 485, 936
BspT107I GGYRCC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 755
BsrGI TGTACA 1 cut(s) 132
BsrI ACTGG 2 cut(s) 184, 567
BssECI CCNNGG 7 cut(s) 500, 622, 623, 740, 767, 788, 842
BssMI GATC 3 cut(s) 28, 490, 928
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 2 cut(s) 500, 788
Bst2UI CCWGG 6 cut(s) 109, 537, 624, 768, 843, 867
Bst4CI ACNGT 3 cut(s) 681, 696, 744
Bst6I CTCTTC 2 cut(s) 62, 321
BstACI GRCGYC 1 cut(s) 105
BstAUI TGTACA 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 5 cut(s) 126, 369, 454, 516, 552
BstDSI CCRYGG 2 cut(s) 740, 788
BstEII GGTNACC 1 cut(s) 744
BstF5I GGATG 4 cut(s) 379, 584, 707, 777
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 3 cut(s) 31, 493, 931
BstMAI GTCTC 2 cut(s) 555, 741
BstMBI GATC 3 cut(s) 28, 490, 928
BstMWI GCNNNNNNNGC 2 cut(s) 553, 626
BstNI CCWGG 6 cut(s) 109, 537, 624, 768, 843, 867
BstNSI RCATGY 2 cut(s) 22, 635
BstPI GGTNACC 1 cut(s) 744
BstSCI CCNGG 6 cut(s) 107, 535, 622, 766, 841, 865
BstSFI CTRYAG 2 cut(s) 321, 644
BstSLI GKGCMC 1 cut(s) 631
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
Bsu36I CCTNAGG 1 cut(s) 126
BsuRI GGCC 4 cut(s) 327, 364, 822, 847
BtgI CCRYGG 2 cut(s) 740, 788
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 4 cut(s) 379, 584, 707, 777
Cac8I GCNNGC 1 cut(s) 325
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 6 cut(s) 326, 665, 792, 820, 839, 845
CseI GACGC 1 cut(s) 113
Csp6I GTAC 2 cut(s) 133, 469
CviAII CATG 5 cut(s) 19, 413, 632, 724, 789
CviQI GTAC 2 cut(s) 133, 469
DdeI CTNAG 5 cut(s) 126, 369, 454, 516, 552
DpnI GATC 3 cut(s) 30, 492, 930
DpnII GATC 3 cut(s) 28, 490, 928
Eam1104I CTCTTC 2 cut(s) 62, 321
EarI CTCTTC 2 cut(s) 62, 321
EciI GGCGGA 1 cut(s) 213
Eco130I CCWWGG 2 cut(s) 500, 788
Eco147I AGGCCT 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 665, 792, 839
Eco57I CTGAAG 2 cut(s) 458, 876
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 607
Eco91I GGTNACC 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 845
EcoO65I GGTNACC 1 cut(s) 744
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 6 cut(s) 107, 535, 622, 766, 841, 865
EcoT14I CCWWGG 2 cut(s) 500, 788
EcoT22I ATGCAT 1 cut(s) 24
ErhI CCWWGG 2 cut(s) 500, 788
FaeI CATG 5 cut(s) 22, 416, 635, 727, 792
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 5 cut(s) 18, 412, 631, 723, 788
FbaI TGATCA 1 cut(s) 28
FokI GGATG 4 cut(s) 366, 571, 694, 784
FspBI CTAG 2 cut(s) 207, 937
GlaI GCGC 1 cut(s) 636
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 4 cut(s) 327, 364, 822, 847
HapII CCGG 1 cut(s) 157
HgaI GACGC 1 cut(s) 113
HhaI GCGC 1 cut(s) 637
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 5 cut(s) 22, 416, 635, 727, 792
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 4 cut(s) 70, 220, 419, 443
HpaII CCGG 1 cut(s) 157
HphI GGTGA 5 cut(s) 378, 590, 675, 738, 756
Hpy166II GTNNAC 3 cut(s) 43, 133, 572
Hpy188I TCNGA 2 cut(s) 171, 477
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 3 cut(s) 43, 133, 572
HpyAV CCTTC 3 cut(s) 482, 870, 900
HpyCH4III ACNGT 3 cut(s) 681, 696, 744
HpyCH4V TGCA 7 cut(s) 14, 22, 79, 250, 323, 547, 775
HpyF10VI GCNNNNNNNGC 2 cut(s) 553, 626
HpyF3I CTNAG 5 cut(s) 126, 369, 454, 516, 552
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 5 cut(s) 22, 416, 635, 727, 792
HspAI GCGC 1 cut(s) 635
Kpn2I TCCGGA 1 cut(s) 156
KpnI GGTACC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 28, 490, 928
LmnI GCTCC 1 cut(s) 316
LweI GCATC 1 cut(s) 762
MaeI CTAG 2 cut(s) 207, 937
MaeIII GTNAC 3 cut(s) 681, 719, 744
MalI GATC 3 cut(s) 30, 492, 930
MboI GATC 3 cut(s) 28, 490, 928
MboII GAAGA 2 cut(s) 79, 308
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 3 cut(s) 89, 255, 799
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 518
Mph1103I ATGCAT 1 cut(s) 24
MroI TCCGGA 1 cut(s) 156
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 1 cut(s) 296
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 6 cut(s) 109, 537, 624, 768, 843, 867
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 6 cut(s) 109, 537, 624, 768, 843, 867
MwoI GCNNNNNNNGC 2 cut(s) 553, 626
NcoI CCATGG 1 cut(s) 788
NdeII GATC 3 cut(s) 28, 490, 928
NlaIII CATG 5 cut(s) 22, 416, 635, 727, 792
NlaIV GGNNCC 3 cut(s) 339, 470, 794
NmuCI GTSAC 2 cut(s) 681, 744
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 2 cut(s) 22, 635
PasI CCCWGGG 1 cut(s) 623
PceI AGGCCT 1 cut(s) 364
PctI GAATGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 3 cut(s) 220, 419, 443
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
Psp6I CCWGG 6 cut(s) 107, 535, 622, 766, 841, 865
PspEI GGTNACC 1 cut(s) 744
PspGI CCWGG 6 cut(s) 107, 535, 622, 766, 841, 865
PspN4I GGNNCC 3 cut(s) 339, 470, 794
PspPI GGNCC 6 cut(s) 326, 665, 792, 820, 839, 845
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 2 cut(s) 134, 470
RsaNI GTAC 2 cut(s) 133, 469
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 1 cut(s) 296
Sau3AI GATC 3 cut(s) 28, 490, 928
Sau96I GGNCC 6 cut(s) 326, 665, 792, 820, 839, 845
SbfI CCTGCAGG 1 cut(s) 325
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 6 cut(s) 109, 537, 624, 768, 843, 867
SdaI CCTGCAGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 1 cut(s) 762
SfcI CTRYAG 2 cut(s) 321, 644
SinI GGWCC 3 cut(s) 665, 792, 839
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse8387I CCTGCAGG 1 cut(s) 325
Sse9I AATT 3 cut(s) 89, 255, 799
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 2 cut(s) 224, 466
SspMI CTAG 2 cut(s) 207, 937
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 6 cut(s) 107, 535, 622, 766, 841, 865
StyI CCWWGG 2 cut(s) 500, 788
TaaI ACNGT 3 cut(s) 681, 696, 744
TaqI TCGA 1 cut(s) 259
TasI AATT 3 cut(s) 89, 255, 799
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 3 cut(s) 220, 419, 443
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TseFI GTSAC 2 cut(s) 681, 744
Tsp45I GTSAC 2 cut(s) 681, 744
TspDTI ATGAA 3 cut(s) 629, 674, 740
TspGWI ACGGA 1 cut(s) 577
VpaK11BI GGWCC 3 cut(s) 665, 792, 839
XapI RAATTY 2 cut(s) 89, 255
XceI RCATGY 2 cut(s) 22, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 2 cut(s) 207, 937
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.