FvH4_4g21990

nucleotide exchange factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009449
Physical Location & Seq
Reverse (-)
24926704 .. 24929244
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21990.t1

Sequence Viewer

Length: 1176 bp
ATGGAGCTTCGGAGTTCATGGATGCTCATAGCGTTAACGGCGACGGCGATTATGCTTACGGCCGTTACGGCGGACCGTACTAACAACAAGTCGGGAGGTCTATACTGGTCAACGGCGGAGGAGGAGGCTCAGGCTCAGCTCCAGCCTAGGGTTGACGATTCCGCCGTGGACGCCGACTTCGACGCCGACGGAGGCTTCTCTTCGCTCGATTCGATGTTGCAGTGGGCCTTAGGTCACTCCGATCCCGATCAATTGAAAGAGGCGGCGAAAGAGGTGCAGGGATTGAGCCCTTCCGACCTGAACAAGAAGCGGCTGGAGATTAAGCAGCTCATGGAGGAGCTCAAGACGCCGTCGGATGCTCAGCTGATGAAGGTGGCCATACTCGACCTGAAGAACTCGTCGTTGTCGCTCGAAGATCGGCACCGGGCTCTGGAGGAGCTGCTGGAGCTTGTGGAGCCGATTGACAATGCCAACGATTTGAACAAGCTTGGAGGATTAGCTGTGGTGATTGGAGAGCTTGATCATTCTGATTCCGAGACGAGGAAAATGGCTGCATGGATTATTGGGAAGGCTAGTCAGAACAATCCACTTGTTCAGAAGCAGGTTTTGGAACTTGGGGCACTGTCTAAGCTAATGGAGATGGTAAAATCGAACTCTGTGGAAGAGGCCATTAAGGCAATTTATGCTGTTTCGGCCTTGCTAAGGAACAATATGGCCGGTCAGGAAATGTTCTATGAGGAAGCTGGGGAAAAGCTGCTTCAGGACATTGTGAGTGATTCGAGCATTGGTAACAGACTGCGGATAAGAGCGGTGGTTCTTCTGGGAGATCTTGCAGAGTTTCAGTTAGGAAATACAGAGAAGGATGAGCTGCCCTTTTTCAGTAATCGCATTTTTTTGAAGTCTGTGATTGATCTAACATCAGCTGTTGACCTTCACCTACAGGAAAAGGCCCTCATTGCCATTAAGAATCTACTGCGACTCAAGACAACTGAAGCTCTGGTGTTTAAGGACTTCTGTGGTTTGGATGTTGCATTGGAGAGAATGAGGCAGCAGCTGCAGGACCTAATGGTAGAGGAATACCACAAAGATTATGCAATGGATGTAGAGAGCCTCCGCAATGAAGTGCAGCTGATTTTCCAAAGAAAGCTAGAAAAGGTAACACGGATGACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Fes1

Protein Analysis

392

Amino Acids

43.69

Weight (kDa)

4.8

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fes1 PF08609 69 - 160 1e-17 Nucleotide exchange factor Fes1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 1 cut(s) 420
AccBSI CCGCTC 1 cut(s) 809
AciI CCGC 8 cut(s) 71, 116, 162, 263, 310, 799, 809, 1114
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 3 cut(s) 60, 375, 714
AcuI CTGAAG 3 cut(s) 410, 743, 1011
AcyI GRCGYC 3 cut(s) 171, 183, 347
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 4 cut(s) 148, 430, 540, 702
AgsI TTSAA 3 cut(s) 256, 481, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 590, 622
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 1 cut(s) 236
AlwNI CAGNNNCTG 1 cut(s) 1054
AoxI GGCC 7 cut(s) 60, 225, 375, 666, 693, 714, 948
ApeKI GCWGC 9 cut(s) 325, 439, 551, 754, 868, 1048, 1051, 1054, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspA2I CCTAGG 1 cut(s) 146
AspS9I GGNCC 4 cut(s) 73, 225, 949, 1060
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 517, 926
AvaII GGWCC 2 cut(s) 73, 1060
AvrII CCTAGG 1 cut(s) 146
AxyI CCTNAGG 1 cut(s) 229
BaeGI GKGCMC 1 cut(s) 622
BalI TGGCCA 1 cut(s) 377
BanI GGYRCC 1 cut(s) 420
BanII GRGCYC 3 cut(s) 290, 342, 430
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 9 cut(s) 337, 426, 538, 741, 855, 1041, 1060, 1063, 1138
BccI CCATC 1 cut(s) 634
BceAI ACGGC 8 cut(s) 47, 54, 60, 75, 84, 129, 149, 334
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 3 cut(s) 147, 573, 1148
BfmI CTRYAG 2 cut(s) 938, 1055
BfuAI ACCTGC 1 cut(s) 592
BglI GCCNNNNNGGC 2 cut(s) 68, 674
BglII AGATCT 1 cut(s) 826
BlnI CCTAGG 1 cut(s) 146
BlpI GCTNAGC 2 cut(s) 135, 360
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 2 cut(s) 73, 1060
BmgT120I GGNCC 4 cut(s) 73, 225, 949, 1060
BmiI GGNNCC 2 cut(s) 422, 456
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 2 cut(s) 12, 346
BpmI CTGGAG 4 cut(s) 125, 335, 452, 464
Bpu10I CCTNAGC 2 cut(s) 129, 701
Bpu1102I GCTNAGC 2 cut(s) 135, 360
BpuEI CTTGAG 2 cut(s) 326, 965
BpuMI CCSGG 1 cut(s) 425
BsaBI GATNNNNATC 1 cut(s) 246
BsaHI GRCGYC 3 cut(s) 171, 183, 347
BsaJI CCNNGG 2 cut(s) 146, 165
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 430, 540, 702
Bse118I RCCGGY 1 cut(s) 716
Bse1I ACTGG 1 cut(s) 110
Bse21I CCTNAGG 1 cut(s) 229
Bse3DI GCAATG 3 cut(s) 954, 1101, 1123
Bse8I GATNNNNATC 1 cut(s) 246
BseDI CCNNGG 2 cut(s) 146, 165
BseGI GGATG 6 cut(s) 27, 361, 868, 1030, 1105, 1170
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 4 cut(s) 148, 430, 540, 702
BseMI GCAATG 3 cut(s) 954, 1101, 1123
BseMII CTCAG 3 cut(s) 143, 149, 374
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 4 cut(s) 134, 137, 350, 449
BseSI GKGCMC 1 cut(s) 622
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 9 cut(s) 337, 426, 538, 741, 855, 1041, 1060, 1063, 1138
BseYI CCCAGC 1 cut(s) 743
BsgI GTGCAG 2 cut(s) 296, 1145
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 7 cut(s) 62, 227, 377, 668, 695, 716, 950
BshNI GGYRCC 1 cut(s) 420
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 2 cut(s) 424, 717
BslI CCNNNNNNNGG 4 cut(s) 148, 430, 540, 702
BsmAI GTCTC 1 cut(s) 530
BsmBI CGTCTC 1 cut(s) 530
BsnI GGCC 7 cut(s) 62, 227, 377, 668, 695, 716, 950
Bsp1286I GDGCHC 4 cut(s) 290, 342, 430, 622
Bsp143I GATC 6 cut(s) 241, 247, 415, 520, 826, 910
Bsp1720I GCTNAGC 2 cut(s) 135, 360
BspACI CCGC 8 cut(s) 71, 116, 162, 263, 310, 799, 809, 1114
BspANI GGCC 7 cut(s) 62, 227, 377, 668, 695, 716, 950
BspCNI CTCAG 3 cut(s) 142, 148, 373
BspLI GGNNCC 2 cut(s) 422, 456
BspMAI CTGCAG 1 cut(s) 1059
BspMI ACCTGC 1 cut(s) 592
BspPI GGATC 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 420
BsrBI CCGCTC 1 cut(s) 809
BsrDI GCAATG 3 cut(s) 954, 1101, 1123
BsrFI RCCGGY 1 cut(s) 716
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 2 cut(s) 146, 165
BssMI GATC 6 cut(s) 241, 247, 415, 520, 826, 910
BssNI GRCGYC 3 cut(s) 171, 183, 347
BssT1I CCWWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 77, 624
Bst6I CTCTTC 2 cut(s) 205, 657
BstACI GRCGYC 3 cut(s) 171, 183, 347
BstAPI GCANNNNNTGC 2 cut(s) 683, 1054
BstDEI CTNAG 6 cut(s) 129, 135, 229, 360, 627, 701
BstDSI CCRYGG 1 cut(s) 165
BstENI CCTNNNNNAGG 1 cut(s) 700
BstF5I GGATG 6 cut(s) 27, 361, 868, 1030, 1105, 1170
BstKTI GATC 6 cut(s) 244, 250, 418, 523, 829, 913
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 6 cut(s) 241, 247, 415, 520, 826, 910
BstMCI CGRYCG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 423
BstSFI CTRYAG 2 cut(s) 938, 1055
BstSLI GKGCMC 1 cut(s) 622
BstV1I GCAGC 9 cut(s) 337, 426, 538, 741, 855, 1041, 1060, 1063, 1138
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BstZI CGGCCG 1 cut(s) 60
Bsu36I CCTNAGG 1 cut(s) 229
BsuRI GGCC 7 cut(s) 62, 227, 377, 668, 695, 716, 950
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 6 cut(s) 27, 361, 868, 1030, 1105, 1170
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 227, 620
BveI ACCTGC 1 cut(s) 592
CaiI CAGNNNCTG 1 cut(s) 1054
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 4 cut(s) 73, 225, 949, 1060
CpoI CGGWCCG 1 cut(s) 73
CseI GACGC 3 cut(s) 179, 191, 355
Csp6I GTAC 1 cut(s) 78
CspI CGGWCCG 1 cut(s) 73
CviAII CATG 4 cut(s) 18, 331, 555, 1173
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 6 cut(s) 129, 135, 229, 360, 627, 701
DpnI GATC 6 cut(s) 243, 249, 417, 522, 828, 912
DpnII GATC 6 cut(s) 241, 247, 415, 520, 826, 910
EaeI YGGCCR 3 cut(s) 60, 375, 714
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 2 cut(s) 205, 657
EarI CTCTTC 2 cut(s) 205, 657
EciI GGCGGA 3 cut(s) 86, 131, 151
Ecl136II GAGCTC 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 3 cut(s) 290, 342, 430
Eco47I GGWCC 2 cut(s) 73, 1060
Eco52I CGGCCG 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 3 cut(s) 410, 743, 1011
Eco81I CCTNAGG 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 340
EcoNI CCTNNNNNAGG 1 cut(s) 700
EcoO109I RGGNCCY 2 cut(s) 949, 1060
EcoT14I CCWWGG 1 cut(s) 146
EcoT38I GRGCYC 3 cut(s) 290, 342, 430
ErhI CCWWGG 1 cut(s) 146
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 4 cut(s) 21, 334, 558, 1176
FatI CATG 4 cut(s) 17, 330, 554, 1172
FbaI TGATCA 1 cut(s) 520
FokI GGATG 5 cut(s) 34, 368, 875, 1037, 1112
FriOI GRGCYC 3 cut(s) 290, 342, 430
FspBI CTAG 3 cut(s) 147, 573, 1148
GsaI CCCAGC 1 cut(s) 747
GsuI CTGGAG 4 cut(s) 125, 335, 452, 464
HaeIII GGCC 7 cut(s) 62, 227, 377, 668, 695, 716, 950
HapII CCGG 2 cut(s) 424, 717
HgaI GACGC 3 cut(s) 179, 191, 355
Hin1I GRCGYC 3 cut(s) 171, 183, 347
Hin1II CATG 4 cut(s) 21, 334, 558, 1176
HincII GTYRAC 4 cut(s) 36, 111, 154, 928
HindII GTYRAC 4 cut(s) 36, 111, 154, 928
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 6 cut(s) 158, 209, 530, 776, 967, 978
HpaI GTTAAC 1 cut(s) 36
HpaII CCGG 2 cut(s) 424, 717
HphI GGTGA 2 cut(s) 517, 926
Hpy166II GTNNAC 5 cut(s) 36, 111, 154, 169, 928
Hpy188I TCNGA 8 cut(s) 12, 241, 295, 355, 529, 535, 579, 597
Hpy188III TCNNGA 7 cut(s) 93, 245, 343, 431, 722, 761, 982
Hpy8I GTNNAC 5 cut(s) 36, 111, 154, 169, 928
Hpy99I CGWCG 5 cut(s) 46, 185, 191, 355, 403
HpyAV CCTTC 5 cut(s) 300, 364, 562, 853, 941
HpyCH4III ACNGT 2 cut(s) 77, 624
HpyCH4V TGCA 9 cut(s) 220, 277, 554, 833, 1031, 1057, 1094, 1126, 1172
HpyF3I CTNAG 6 cut(s) 129, 135, 229, 360, 627, 701
Hsp92I GRCGYC 3 cut(s) 171, 183, 347
Hsp92II CATG 4 cut(s) 21, 334, 558, 1176
Ksp22I TGATCA 1 cut(s) 520
KspAI GTTAAC 1 cut(s) 36
Kzo9I GATC 6 cut(s) 241, 247, 415, 520, 826, 910
LmnI GCTCC 6 cut(s) 4, 144, 337, 436, 445, 454
Lsp1109I GCAGC 9 cut(s) 337, 426, 538, 741, 855, 1041, 1060, 1063, 1138
LweI GCATC 2 cut(s) 12, 346
MaeI CTAG 3 cut(s) 147, 573, 1148
MaeIII GTNAC 4 cut(s) 64, 233, 788, 1156
MalI GATC 6 cut(s) 243, 249, 417, 522, 828, 912
MbiI CCGCTC 1 cut(s) 809
MboI GATC 6 cut(s) 241, 247, 415, 520, 826, 910
MboII GAAGA 5 cut(s) 192, 403, 425, 674, 809
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 4 cut(s) 290, 342, 430, 622
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 2 cut(s) 251, 678
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 1 cut(s) 972
MmeI TCCRAC 2 cut(s) 318, 333
Mox20I TGGCCA 1 cut(s) 377
MscI TGGCCA 1 cut(s) 377
MseI TTAA 5 cut(s) 35, 321, 672, 963, 1005
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 4 cut(s) 364, 923, 1054, 1129
MspI CCGG 2 cut(s) 424, 717
MspR9I CCNGG 1 cut(s) 425
MunI CAATTG 1 cut(s) 251
NciI CCSGG 1 cut(s) 425
NdeII GATC 6 cut(s) 241, 247, 415, 520, 826, 910
NlaIII CATG 4 cut(s) 21, 334, 558, 1176
NlaIV GGNNCC 2 cut(s) 422, 456
NmuCI GTSAC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 4 cut(s) 44, 162, 177, 209
PfeI GAWTC 5 cut(s) 158, 209, 530, 776, 967
PflFI GACNNNGTC 1 cut(s) 349
PleI GAGTC 1 cut(s) 972
PpsI GAGTC 1 cut(s) 972
PpuMI RGGWCCY 1 cut(s) 1060
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 1060
PspFI CCCAGC 1 cut(s) 743
PspN4I GGNNCC 2 cut(s) 422, 456
PspPI GGNCC 4 cut(s) 73, 225, 949, 1060
PspPPI RGGWCCY 1 cut(s) 1060
PstI CTGCAG 1 cut(s) 1059
PstNI CAGNNNCTG 1 cut(s) 1054
PsuI RGATCY 1 cut(s) 826
PsyI GACNNNGTC 1 cut(s) 349
PvuII CAGCTG 4 cut(s) 364, 923, 1054, 1129
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
Rsr2I CGGWCCG 1 cut(s) 73
RsrII CGGWCCG 1 cut(s) 73
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 5 cut(s) 35, 321, 672, 963, 1005
Sau3AI GATC 6 cut(s) 241, 247, 415, 520, 826, 910
Sau96I GGNCC 4 cut(s) 73, 225, 949, 1060
SchI GAGTC 1 cut(s) 972
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 4 cut(s) 290, 342, 430, 622
SfaNI GCATC 2 cut(s) 12, 346
SfcI CTRYAG 2 cut(s) 938, 1055
SinI GGWCC 2 cut(s) 73, 1060
SmlI CTYRAG 2 cut(s) 341, 980
SmoI CTYRAG 2 cut(s) 341, 980
Sse9I AATT 2 cut(s) 251, 678
SsiI CCGC 8 cut(s) 71, 116, 162, 263, 310, 799, 809, 1114
SspMI CTAG 3 cut(s) 147, 573, 1148
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 77, 624
TaqI TCGA 7 cut(s) 180, 207, 212, 384, 411, 650, 779
TasI AATT 2 cut(s) 251, 678
TauI GCSGC 2 cut(s) 266, 313
TfiI GAWTC 5 cut(s) 158, 209, 530, 776, 967
Tru1I TTAA 5 cut(s) 35, 321, 672, 963, 1005
Tru9I TTAA 5 cut(s) 35, 321, 672, 963, 1005
TscAI CASTG 2 cut(s) 227, 627
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 9 cut(s) 325, 439, 551, 754, 868, 1048, 1051, 1054, 1126
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 3 cut(s) 6, 383, 1134
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 2 cut(s) 227, 627
Tth111I GACNNNGTC 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 73, 1060
XagI CCTNNNNNAGG 1 cut(s) 700
XmaJI CCTAGG 1 cut(s) 146
XspI CTAG 3 cut(s) 147, 573, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.