Rh4AG280600

nucleotide exchange factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009449
Physical Location & Seq
Reverse (-)
59589687 .. 59592323
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG280600.1

Sequence Viewer

Length: 987 bp
ATGGAGCTTCGGAGTTCATGGATGGTAGCGTTAACGTTGACGGCGGTGATGGTAACGGCCATAACGGCAGACCGGAGTAATAACAAGTCGGGAGGTTTATACTGGTCAACGGCGGAAGAGGAGGCTCAGGCTCAGCTTCAGCCTAGGGTTGACGATTCCGCCGTGGACGCCGACTTCGACGCCGACGGAGGCTTCTCCTCGCTCGATTCGATGTTGCAGTGGGCTTTGGGTCACTCCGATCCCGATCAATTGAAAGAAGCGGCGAAAGAGGTGCAGGGATTGAACCCTTCCGACCTGAACAAGCGGCGGCTCGAGATAAAGCAGCTCATGGAGGAGCTCAAAACGCCGTCGGATGCTCAGCTGATGAAGGTGGCGATACTGGACCTGAAGAACTCGTCGTTGTCGCTGGAAGATCGGCACCGGGCTTTGGAGGAGCTGCTGGAGCTTGTTGAGCCGATTGACAATGCAAATGATTTGAACAAGCTTGGAGGACTTGCTGTGGTGATAGGAGAGCTTGATCATTCTGATTCAGAGACGAGGAAAATGGCTGCATGGATTATTGGGAAGGCTAGTCAGAACAATCCACTTGTTCAGAAGCAGGTTTTGGAACTTGGGGCACTGTCAAAGCTAATGAAGATGGTAAAATCGAGCTTTGCGGAAGAAGCCATTAAGGCAATTTATGCTGTTTCAGCCTTGCTGAGGAATAATGTAGCTGGTCAGGAAATGTTCTATGAGGAAACTGGGCAAAAACTGCTTCAGGACATTGTGAGTGATTCGAGCATTGGTAACAGACTGCGCATAAGAGCTGTGGTTCTTCTGGGAGATCTTGCAGAGTTTCAATTAGGAAATACGGATAAGGACGAGCGGCCCTTTTTCAGTAATCGCATTTTCTTGAAGTCTGTTGTTGATCTAACATCAGCTGCTGATCTTCACCTACAGGAAAAGGTGAGAAAGGCTAGAATCTTCTTTTCAATATATCTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Fes1

Protein Analysis

328

Amino Acids

36.36

Weight (kDa)

4.92

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fes1 PF08609 68 - 159 8.9e-18 Nucleotide exchange factor Fes1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 417
AccBSI CCGCTC 1 cut(s) 865
AciI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 656, 865
AclI AACGTT 1 cut(s) 35
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 3 cut(s) 122, 407, 740
AcyI GRCGYC 2 cut(s) 168, 180
AfiI CCNNNNNNNGG 2 cut(s) 427, 699
AgsI TTSAA 6 cut(s) 253, 283, 478, 839, 895, 972
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 923
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 2 cut(s) 57, 866
ApeKI GCWGC 4 cut(s) 322, 436, 548, 920
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspA2I CCTAGG 1 cut(s) 143
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 2 cut(s) 382, 867
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 4 cut(s) 58, 514, 923, 958
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 382
AvrII CCTAGG 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 417
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 698
BbvI GCAGC 4 cut(s) 334, 423, 535, 907
BccI CCATC 3 cut(s) 16, 43, 631
BceAI ACGGC 6 cut(s) 57, 72, 81, 126, 146, 331
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 3 cut(s) 144, 570, 957
BfmI CTRYAG 1 cut(s) 935
BfuAI ACCTGC 1 cut(s) 589
BglI GCCNNNNNGGC 2 cut(s) 65, 671
BglII AGATCT 1 cut(s) 823
BisI GCNGC 8 cut(s) 261, 305, 308, 323, 437, 549, 866, 921
BlnI CCTAGG 1 cut(s) 143
BlpI GCTNAGC 2 cut(s) 132, 357
BlsI GCNGC 8 cut(s) 262, 306, 309, 324, 438, 550, 867, 922
Bme1390I CCNGG 1 cut(s) 422
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 382, 867
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 1 cut(s) 422
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 1 cut(s) 343
BmuI ACTGGG 1 cut(s) 750
BpmI CTGGAG 1 cut(s) 461
Bpu10I CCTNAGC 2 cut(s) 126, 698
Bpu1102I GCTNAGC 2 cut(s) 132, 357
BpuMI CCSGG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 243
BsaHI GRCGYC 2 cut(s) 168, 180
BsaJI CCNNGG 2 cut(s) 143, 162
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 699
Bse1I ACTGG 3 cut(s) 107, 384, 745
Bse8I GATNNNNATC 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 143, 162
BseGI GGATG 2 cut(s) 27, 358
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 2 cut(s) 427, 699
BseMII CTCAG 4 cut(s) 140, 146, 371, 689
BseNI ACTGG 3 cut(s) 107, 384, 745
BseRI GAGGAG 4 cut(s) 134, 187, 347, 446
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 4 cut(s) 334, 423, 535, 907
BsgI GTGCAG 1 cut(s) 293
BshFI GGCC 2 cut(s) 59, 868
BshNI GGYRCC 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 2 cut(s) 73, 421
BslI CCNNNNNNNGG 2 cut(s) 427, 699
BsmAI GTCTC 1 cut(s) 527
BsmBI CGTCTC 1 cut(s) 527
BsnI GGCC 2 cut(s) 59, 868
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 339, 619
Bsp143I GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
Bsp1720I GCTNAGC 2 cut(s) 132, 357
BspACI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 656, 865
BspANI GGCC 2 cut(s) 59, 868
BspCNI CTCAG 4 cut(s) 139, 145, 370, 690
BspLI GGNNCC 1 cut(s) 419
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 417
BsrBI CCGCTC 1 cut(s) 865
BsrI ACTGG 3 cut(s) 107, 384, 745
BssECI CCNNGG 2 cut(s) 143, 162
BssMI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
BssNI GRCGYC 2 cut(s) 168, 180
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 621
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 2 cut(s) 168, 180
BstAPI GCANNNNNTGC 2 cut(s) 680, 751
BstDEI CTNAG 4 cut(s) 126, 132, 357, 698
BstDSI CCRYGG 1 cut(s) 162
BstENI CCTNNNNNAGG 1 cut(s) 697
BstF5I GGATG 2 cut(s) 27, 358
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 7 cut(s) 241, 247, 415, 520, 826, 910, 928
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 1 cut(s) 935
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 4 cut(s) 334, 423, 535, 907
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BsuRI GGCC 2 cut(s) 59, 868
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 27, 358
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 224, 617
BveI ACCTGC 1 cut(s) 589
CaiI CAGNNNCTG 1 cut(s) 923
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 382, 867
CseI GACGC 2 cut(s) 176, 188
CviAII CATG 3 cut(s) 18, 328, 552
DdeI CTNAG 4 cut(s) 126, 132, 357, 698
DpnI GATC 7 cut(s) 240, 246, 414, 519, 825, 909, 927
DpnII GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 2 cut(s) 128, 148
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 3 cut(s) 122, 407, 740
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 697
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 143
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 3 cut(s) 21, 331, 555
FaiI YATR 9 cut(s) 19, 62, 100, 329, 553, 681, 732, 800, 976
FatI CATG 3 cut(s) 17, 327, 551
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 8 cut(s) 261, 305, 308, 323, 437, 549, 866, 921
FokI GGATG 2 cut(s) 34, 365
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 8 cut(s) 261, 305, 308, 323, 437, 549, 866, 921
FspBI CTAG 3 cut(s) 144, 570, 957
FspI TGCGCA 1 cut(s) 797
GlaI GCGC 1 cut(s) 797
GluI GCNGC 8 cut(s) 261, 305, 308, 323, 437, 549, 866, 921
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 2 cut(s) 59, 868
HapII CCGG 2 cut(s) 73, 421
HgaI GACGC 2 cut(s) 176, 188
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 2 cut(s) 168, 180
Hin1II CATG 3 cut(s) 21, 331, 555
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 4 cut(s) 33, 39, 108, 151
HindII GTYRAC 4 cut(s) 33, 39, 108, 151
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 5 cut(s) 155, 206, 527, 773, 960
HpaI GTTAAC 1 cut(s) 33
HpaII CCGG 2 cut(s) 73, 421
HphI GGTGA 4 cut(s) 58, 514, 923, 958
Hpy166II GTNNAC 5 cut(s) 33, 39, 108, 151, 166
Hpy188I TCNGA 8 cut(s) 12, 238, 292, 352, 526, 532, 576, 594
Hpy188III TCNNGA 6 cut(s) 90, 242, 313, 719, 758, 892
Hpy8I GTNNAC 5 cut(s) 33, 39, 108, 151, 166
Hpy99I CGWCG 4 cut(s) 182, 188, 352, 400
HpyAV CCTTC 3 cut(s) 297, 361, 559
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 5 cut(s) 217, 274, 467, 551, 830
HpyF3I CTNAG 4 cut(s) 126, 132, 357, 698
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 2 cut(s) 168, 180
Hsp92II CATG 3 cut(s) 21, 331, 555
HspAI GCGC 1 cut(s) 796
Ksp22I TGATCA 1 cut(s) 517
KspAI GTTAAC 1 cut(s) 33
Kzo9I GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
LmnI GCTCC 4 cut(s) 4, 334, 433, 442
Lsp1109I GCAGC 4 cut(s) 334, 423, 535, 907
LweI GCATC 1 cut(s) 343
MaeI CTAG 3 cut(s) 144, 570, 957
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 3 cut(s) 52, 230, 785
MalI GATC 7 cut(s) 240, 246, 414, 519, 825, 909, 927
MbiI CCGCTC 1 cut(s) 865
MboI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
MboII GAAGA 8 cut(s) 128, 400, 422, 646, 671, 806, 920, 955
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 2 cut(s) 339, 619
MluCI AATT 3 cut(s) 248, 675, 839
MmeI TCCRAC 2 cut(s) 315, 330
MseI TTAA 3 cut(s) 32, 669, 985
MspA1I CMGCKG 2 cut(s) 361, 920
MspI CCGG 2 cut(s) 73, 421
MspR9I CCNGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 248
NciI CCSGG 1 cut(s) 422
NdeII GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
NlaIII CATG 3 cut(s) 21, 331, 555
NlaIV GGNNCC 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 230
NsbI TGCGCA 1 cut(s) 797
PaeR7I CTCGAG 1 cut(s) 311
PcsI WCGNNNNNNNCGW 3 cut(s) 159, 174, 206
PfeI GAWTC 5 cut(s) 155, 206, 527, 773, 960
PkrI GCNGC 8 cut(s) 262, 306, 309, 324, 438, 550, 867, 922
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 2 cut(s) 382, 867
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 1 cut(s) 823
PvuII CAGCTG 2 cut(s) 361, 920
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 3 cut(s) 32, 669, 985
SatI GCNGC 8 cut(s) 261, 305, 308, 323, 437, 549, 866, 921
Sau3AI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
Sau96I GGNCC 2 cut(s) 382, 867
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 2 cut(s) 339, 619
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 935
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 1 cut(s) 382
SlaI CTCGAG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 3 cut(s) 248, 675, 839
SsiI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 656, 865
SspMI CTAG 3 cut(s) 144, 570, 957
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 1 cut(s) 621
TaiI ACGT 1 cut(s) 38
TaqI TCGA 6 cut(s) 177, 204, 209, 312, 647, 776
TasI AATT 3 cut(s) 248, 675, 839
TauI GCSGC 4 cut(s) 263, 307, 310, 868
TfiI GAWTC 5 cut(s) 155, 206, 527, 773, 960
Tru1I TTAA 3 cut(s) 32, 669, 985
Tru9I TTAA 3 cut(s) 32, 669, 985
TscAI CASTG 2 cut(s) 224, 624
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 4 cut(s) 322, 436, 548, 920
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 6, 380, 647
TspGWI ACGGA 2 cut(s) 201, 866
TspRI CASTG 2 cut(s) 224, 624
VpaK11BI GGWCC 1 cut(s) 382
XagI CCTNNNNNAGG 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 311
XmaJI CCTAGG 1 cut(s) 143
XspI CTAG 3 cut(s) 144, 570, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.