MD13G1135300.v1.1

nucleotide exchange factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009449
Physical Location & Seq
Forward (+)
10428729 .. 10431084
2356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1135300.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGCGCGCGCGAGTTCATACATCTTGGTACTGATGGTGGTTGCGATGGCCGTGAGGGCGGAGCGAAATAGTAACAATACGGGAAGGGTATATTGGTCCAGTGCAGAAGACGAGGCGCAGGCTCAGACTCGGACTCAGCCTCGGGACGACGATACCGAGGTCGCTGACCTTGACGCCGACGGTGGCTTCTCTTCTCTGGAGGGAATGTTACAGTGGGCCATAGGCCACTCCGATCCAGTCCAATTGAAAGAGAAGGCGCGAGATGTCGAGCGATTGTCTCCCGCCGACCTAAACGACCGCCGGCTAGAAATAAAGCAGCTCATGGACGAGCTGAAGACGCCGTCGGACGCGCAGCTGATGCAGATTGCGATTGACGACTTGAGTAACTCGTCGCTGTCGTTGGAAGATCGGCACCGTGCTCTGGAGGAACTGCTGGTGCTGGTGGAGCCGATTGATAACGCAAATGATTTGGACAAGCTTGGGGGGATTGCTGCGGTTATAGGAGAGCTTAATCACGGTGACGCAGATACAAGAAAATTGGCTGCTTGGGTACTTGGGACGGCCAGTCAGAACAACCCAGTTGTTCAGAAGCAGGTTCTGGAGCACGGGGCATTGCCCAAGCTAGTAAAGATGGTGAAATCTAGCTTTGTAGAAGAAGCTACTAAGGCATTGTTTGCTGTTTCGGCGTTGATCCGAAACAATGTAGCTGGGCAAGAGTTGTTCTACGTGGAAGATGGGCATTTAGTCCTTCAGGACATTATGAGTGAAGCAAGCATTGATATCAGACTGCGAAGGAAAGCGGTGTTTCTTCTTGGTGACCTTGCTGAGTGTCAATTGGAAAACACGGATAAGGATGAGCTGCCCTTTTTCAGTAATCGGGTTTTCTTGAAGTCTGTGGTTGATCTAACATCATCTGCTGACCTTGATTTGCAGGAGAAGGCCCTCGTCGCACTTAAGAATCTTCTACAACTCAGGACAACCGAAGCTCTGGTTTTTAAGAACTTTTGTGGGTTGGATGGTGCCTTGGAGAGATTGAGACAGCGACTGCAGGGTTTAATGGTAGAGGAAGACCACAGAGATTATGCGGTGGATGTCGAAAGCATCCGCAGTGAAGTGCAGCAGATTTTCAATAGAAAGTTGGAAAAGGTAACGCGAGTTCCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Fes1

Protein Analysis

389

Amino Acids

43.26

Weight (kDa)

4.81

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fes1 PF08609 66 - 157 4.7e-18 Nucleotide exchange factor Fes1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 583
AccB1I GGYRCC 2 cut(s) 411, 1019
AccII CGCG 6 cut(s) 7, 9, 11, 259, 350, 1153
AciI CCGC 7 cut(s) 59, 282, 298, 494, 800, 1085, 1105
AclWI GGATC 2 cut(s) 227, 685
AcoI YGGCCR 2 cut(s) 48, 561
AcuI CTGAAG 2 cut(s) 353, 734
AcyI GRCGYC 2 cut(s) 174, 338
AfaI GTAC 2 cut(s) 30, 552
AfiI CCNNNNNNNGG 1 cut(s) 421
AflII CTTAAG 1 cut(s) 953
AgsI TTSAA 3 cut(s) 247, 889, 1129
AhdI GACNNNNNGTC 1 cut(s) 564
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
Alw21I GWGCWC 2 cut(s) 421, 606
Alw26I GTCTC 2 cut(s) 282, 1030
AlwI GGATC 2 cut(s) 227, 685
AlwNI CAGNNNCTG 2 cut(s) 598, 1045
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 5 cut(s) 48, 216, 223, 561, 939
ApeKI GCWGC 6 cut(s) 316, 352, 491, 542, 859, 1117
AspLEI GCGC 6 cut(s) 7, 9, 11, 118, 259, 352
AspS9I GGNCC 3 cut(s) 96, 216, 940
AsuHPI GGTGA 3 cut(s) 530, 646, 827
AvaI CYCGRG 1 cut(s) 141
AvaII GGWCC 1 cut(s) 96
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BanI GGYRCC 2 cut(s) 411, 1019
BbsI GAAGAC 3 cut(s) 114, 341, 1074
Bbv12I GWGCWC 2 cut(s) 421, 606
BbvI GCAGC 6 cut(s) 328, 364, 478, 529, 846, 1129
BccI CCATC 5 cut(s) 28, 40, 625, 728, 1010
BceAI ACGGC 3 cut(s) 35, 325, 576
BcoDI GTCTC 2 cut(s) 282, 1030
BfaI CTAG 3 cut(s) 305, 623, 642
BfmI CTRYAG 1 cut(s) 1046
BfrI CTTAAG 1 cut(s) 953
BfuAI ACCTGC 1 cut(s) 583
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 6 cut(s) 317, 353, 492, 543, 860, 1118
BlsI GCNGC 6 cut(s) 318, 354, 493, 544, 861, 1119
Bme18I GGWCC 1 cut(s) 96
BmeRI GACNNNNNGTC 1 cut(s) 564
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 3 cut(s) 96, 216, 940
BmiI GGNNCC 3 cut(s) 413, 447, 1021
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 2 cut(s) 348, 1110
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 3 cut(s) 114, 341, 1074
BpmI CTGGAG 3 cut(s) 218, 443, 620
BpuEI CTTGAG 1 cut(s) 400
BsaAI YACGTR 1 cut(s) 727
BsaHI GRCGYC 2 cut(s) 174, 338
BsaJI CCNNGG 3 cut(s) 140, 156, 1023
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse118I RCCGGY 1 cut(s) 300
Bse1I ACTGG 4 cut(s) 99, 236, 564, 578
Bse3DI GCAATG 1 cut(s) 611
BseDI CCNNGG 3 cut(s) 140, 156, 1023
BseGI GGATG 4 cut(s) 859, 1021, 1096, 1101
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 1 cut(s) 611
BseMII CTCAG 4 cut(s) 137, 149, 816, 985
BseNI ACTGG 4 cut(s) 99, 236, 564, 578
BsePI GCGCGC 2 cut(s) 5, 7
BseXI GCAGC 6 cut(s) 328, 364, 478, 529, 846, 1129
BseYI CCCAGC 1 cut(s) 707
BsgI GTGCAG 2 cut(s) 123, 1136
Bsh1236I CGCG 6 cut(s) 7, 9, 11, 259, 350, 1153
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 5 cut(s) 50, 218, 225, 563, 941
BshNI GGYRCC 2 cut(s) 411, 1019
BsiEI CGRYCG 1 cut(s) 298
BsiHKAI GWGCWC 2 cut(s) 421, 606
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 1 cut(s) 301
BslFI GGGAC 2 cut(s) 158, 571
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 2 cut(s) 282, 1030
BsmFI GGGAC 2 cut(s) 158, 571
BsnI GGCC 5 cut(s) 50, 218, 225, 563, 941
BsoBI CYCGRG 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 421, 606
Bsp143I GATC 4 cut(s) 232, 406, 690, 901
BspACI CCGC 7 cut(s) 59, 282, 298, 494, 800, 1085, 1105
BspANI GGCC 5 cut(s) 50, 218, 225, 563, 941
BspCNI CTCAG 4 cut(s) 136, 148, 817, 984
BspFNI CGCG 6 cut(s) 7, 9, 11, 259, 350, 1153
BspLI GGNNCC 3 cut(s) 413, 447, 1021
BspMAI CTGCAG 1 cut(s) 1050
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 2 cut(s) 227, 685
BspT107I GGYRCC 2 cut(s) 411, 1019
BspTI CTTAAG 1 cut(s) 953
BsrDI GCAATG 1 cut(s) 611
BsrFI RCCGGY 1 cut(s) 300
BsrI ACTGG 4 cut(s) 99, 236, 564, 578
BssAI RCCGGY 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 140, 156, 1023
BssHII GCGCGC 2 cut(s) 5, 7
BssMI GATC 4 cut(s) 232, 406, 690, 901
BssNI GRCGYC 2 cut(s) 174, 338
BssT1I CCWWGG 1 cut(s) 1023
Bst4CI ACNGT 4 cut(s) 182, 213, 416, 518
Bst6I CTCTTC 1 cut(s) 196
BstACI GRCGYC 2 cut(s) 174, 338
BstAFI CTTAAG 1 cut(s) 953
BstAPI GCANNNNNTGC 2 cut(s) 358, 674
BstBAI YACGTR 1 cut(s) 727
BstC8I GCNNGC 5 cut(s) 7, 9, 120, 302, 772
BstDEI CTNAG 5 cut(s) 123, 135, 663, 825, 971
BstEII GGTNACC 1 cut(s) 815
BstF5I GGATG 4 cut(s) 859, 1021, 1096, 1101
BstFNI CGCG 6 cut(s) 7, 9, 11, 259, 350, 1153
BstHHI GCGC 6 cut(s) 7, 9, 11, 118, 259, 352
BstKTI GATC 4 cut(s) 235, 409, 693, 904
BstMAI GTCTC 2 cut(s) 282, 1030
BstMBI GATC 4 cut(s) 232, 406, 690, 901
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 8 cut(s) 56, 337, 358, 445, 665, 674, 683, 947
BstPI GGTNACC 1 cut(s) 815
BstSFI CTRYAG 1 cut(s) 1046
BstUI CGCG 6 cut(s) 7, 9, 11, 259, 350, 1153
BstV1I GCAGC 6 cut(s) 328, 364, 478, 529, 846, 1129
BstV2I GAAGAC 3 cut(s) 114, 341, 1074
BsuRI GGCC 5 cut(s) 50, 218, 225, 563, 941
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 4 cut(s) 859, 1021, 1096, 1101
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 3 cut(s) 106, 218, 1114
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 5 cut(s) 7, 9, 120, 302, 772
CaiI CAGNNNCTG 2 cut(s) 598, 1045
CfoI GCGC 6 cut(s) 7, 9, 11, 118, 259, 352
Cfr10I RCCGGY 1 cut(s) 300
Cfr13I GGNCC 3 cut(s) 96, 216, 940
CseI GACGC 4 cut(s) 182, 346, 356, 530
Csp6I GTAC 2 cut(s) 29, 551
CviAII CATG 2 cut(s) 322, 1164
CviQI GTAC 2 cut(s) 29, 551
DdeI CTNAG 5 cut(s) 123, 135, 663, 825, 971
DpnI GATC 4 cut(s) 234, 408, 692, 903
DpnII GATC 4 cut(s) 232, 406, 690, 901
DriI GACNNNNNGTC 1 cut(s) 564
EaeI YGGCCR 2 cut(s) 48, 561
Eam1104I CTCTTC 1 cut(s) 196
Eam1105I GACNNNNNGTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 196
EciI GGCGGA 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 1023
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 96
Eco57I CTGAAG 2 cut(s) 353, 734
Eco88I CYCGRG 1 cut(s) 141
Eco91I GGTNACC 1 cut(s) 815
EcoO109I RGGNCCY 1 cut(s) 940
EcoO65I GGTNACC 1 cut(s) 815
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 1023
ErhI CCWWGG 1 cut(s) 1023
FaeI CATG 2 cut(s) 325, 1167
FaiI YATR 8 cut(s) 19, 91, 221, 323, 500, 761, 1083, 1165
FaqI GGGAC 2 cut(s) 158, 571
FatI CATG 2 cut(s) 321, 1163
FauI CCCGC 1 cut(s) 289
Fnu4HI GCNGC 6 cut(s) 317, 353, 492, 543, 860, 1118
FokI GGATG 4 cut(s) 866, 1028, 1088, 1103
Fsp4HI GCNGC 6 cut(s) 317, 353, 492, 543, 860, 1118
FspBI CTAG 3 cut(s) 305, 623, 642
GlaI GCGC 6 cut(s) 6, 8, 10, 117, 258, 351
GluI GCNGC 6 cut(s) 317, 353, 492, 543, 860, 1118
GsaI CCCAGC 1 cut(s) 711
GsuI CTGGAG 3 cut(s) 218, 443, 620
HaeIII GGCC 5 cut(s) 50, 218, 225, 563, 941
HapII CCGG 1 cut(s) 301
HgaI GACGC 4 cut(s) 182, 346, 356, 530
HhaI GCGC 6 cut(s) 7, 9, 11, 118, 259, 352
Hin1I GRCGYC 2 cut(s) 174, 338
Hin1II CATG 2 cut(s) 325, 1167
Hin6I GCGC 6 cut(s) 5, 7, 9, 116, 257, 350
HinP1I GCGC 6 cut(s) 5, 7, 9, 116, 257, 350
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 3 cut(s) 127, 133, 958
HpaII CCGG 1 cut(s) 301
HphI GGTGA 3 cut(s) 530, 646, 827
Hpy188I TCNGA 8 cut(s) 126, 132, 232, 346, 570, 588, 695, 785
Hpy188III TCNNGA 7 cut(s) 143, 197, 422, 599, 752, 886, 973
Hpy99I CGWCG 5 cut(s) 152, 182, 346, 394, 950
HpyAV CCTTC 5 cut(s) 78, 247, 758, 786, 931
HpyCH4III ACNGT 4 cut(s) 182, 213, 416, 518
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 5 cut(s) 104, 361, 931, 1048, 1117
HpyF10VI GCNNNNNNNGC 8 cut(s) 56, 337, 358, 445, 665, 674, 683, 947
HpyF3I CTNAG 5 cut(s) 123, 135, 663, 825, 971
HpySE526I ACGT 1 cut(s) 726
Hsp92I GRCGYC 2 cut(s) 174, 338
Hsp92II CATG 2 cut(s) 325, 1167
HspAI GCGC 6 cut(s) 5, 7, 9, 116, 257, 350
KroI GCCGGC 1 cut(s) 300
KroNI GCCGGC 1 cut(s) 302
Kzo9I GATC 4 cut(s) 232, 406, 690, 901
LmnI GCTCC 3 cut(s) 61, 445, 601
Lsp1109I GCAGC 6 cut(s) 328, 364, 478, 529, 846, 1129
LweI GCATC 2 cut(s) 348, 1110
MaeI CTAG 3 cut(s) 305, 623, 642
MaeII ACGT 1 cut(s) 726
MaeIII GTNAC 6 cut(s) 71, 207, 383, 518, 815, 1147
MalI GATC 4 cut(s) 234, 408, 692, 903
MauBI CGCGCGCG 1 cut(s) 7
MboI GATC 4 cut(s) 232, 406, 690, 901
MboII GAAGA 9 cut(s) 119, 183, 346, 416, 665, 743, 800, 953, 1079
MfeI CAATTG 2 cut(s) 242, 833
MhlI GDGCHC 2 cut(s) 421, 606
MluCI AATT 3 cut(s) 242, 536, 833
MlyI GAGTC 2 cut(s) 121, 127
MmeI TCCRAC 4 cut(s) 324, 381, 993, 1119
MnlI CCTC 8 cut(s) 48, 106, 150, 151, 193, 418, 953, 1057
MroNI GCCGGC 1 cut(s) 300
MseI TTAA 4 cut(s) 510, 954, 996, 1055
MspA1I CMGCKG 1 cut(s) 355
MspCI CTTAAG 1 cut(s) 953
MspI CCGG 1 cut(s) 301
MunI CAATTG 2 cut(s) 242, 833
MvnI CGCG 6 cut(s) 7, 9, 11, 259, 350, 1153
MwoI GCNNNNNNNGC 8 cut(s) 56, 337, 358, 445, 665, 674, 683, 947
NaeI GCCGGC 1 cut(s) 302
NdeII GATC 4 cut(s) 232, 406, 690, 901
NgoMIV GCCGGC 1 cut(s) 300
NlaIII CATG 2 cut(s) 325, 1167
NlaIV GGNNCC 3 cut(s) 413, 447, 1021
NmuCI GTSAC 2 cut(s) 518, 815
PauI GCGCGC 2 cut(s) 5, 7
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 395
PdiI GCCGGC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 958
PflFI GACNNNGTC 1 cut(s) 340
PkrI GCNGC 6 cut(s) 318, 354, 493, 544, 861, 1119
PleI GAGTC 2 cut(s) 121, 127
PpsI GAGTC 2 cut(s) 121, 127
Ppu21I YACGTR 1 cut(s) 727
PspEI GGTNACC 1 cut(s) 815
PspFI CCCAGC 1 cut(s) 707
PspN4I GGNNCC 3 cut(s) 413, 447, 1021
PspPI GGNCC 3 cut(s) 96, 216, 940
PsrI GAACNNNNNNTAC 1 cut(s) 1140
PstI CTGCAG 1 cut(s) 1050
PstNI CAGNNNCTG 2 cut(s) 598, 1045
PsyI GACNNNGTC 1 cut(s) 340
PteI GCGCGC 2 cut(s) 5, 7
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 2 cut(s) 30, 552
RsaNI GTAC 2 cut(s) 29, 551
SaqAI TTAA 4 cut(s) 510, 954, 996, 1055
SatI GCNGC 6 cut(s) 317, 353, 492, 543, 860, 1118
Sau3AI GATC 4 cut(s) 232, 406, 690, 901
Sau96I GGNCC 3 cut(s) 96, 216, 940
SchI GAGTC 2 cut(s) 121, 127
SduI GDGCHC 2 cut(s) 421, 606
SfaNI GCATC 2 cut(s) 348, 1110
SfcI CTRYAG 1 cut(s) 1046
SinI GGWCC 1 cut(s) 96
SmlI CTYRAG 2 cut(s) 379, 953
SmoI CTYRAG 2 cut(s) 379, 953
Sse9I AATT 3 cut(s) 242, 536, 833
SsiI CCGC 7 cut(s) 59, 282, 298, 494, 800, 1085, 1105
SspMI CTAG 3 cut(s) 305, 623, 642
StyI CCWWGG 1 cut(s) 1023
TaaI ACNGT 4 cut(s) 182, 213, 416, 518
TaiI ACGT 1 cut(s) 729
TaqI TCGA 2 cut(s) 267, 1095
TasI AATT 3 cut(s) 242, 536, 833
TfiI GAWTC 1 cut(s) 958
Tru1I TTAA 4 cut(s) 510, 954, 996, 1055
Tru9I TTAA 4 cut(s) 510, 954, 996, 1055
TscAI CASTG 3 cut(s) 106, 218, 1114
TseFI GTSAC 2 cut(s) 518, 815
TseI GCWGC 6 cut(s) 316, 352, 491, 542, 859, 1117
Tsp45I GTSAC 2 cut(s) 518, 815
TspDTI ATGAA 1 cut(s) 6
TspGWI ACGGA 1 cut(s) 860
TspRI CASTG 3 cut(s) 106, 218, 1114
Tth111I GACNNNGTC 1 cut(s) 340
Vha464I CTTAAG 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 96
XspI CTAG 3 cut(s) 305, 623, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.