Rorug04G0223300

nucleotide exchange factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009449
Physical Location & Seq
Reverse (-)
38351565 .. 38352038
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0223300.1

Sequence Viewer

Length: 474 bp
ATGCCTCAGGTGGAGTTCAAAGAATGGATGCTCGAAAATGCTTTGCATCTAAAGAAGGAACTGTTTGCGAAGCTCTTGATGATTCTATGGGCCTTGTGGAAAAATAAAAACAATTTGTTGTGGAACAGTACAAAGCAATCAGCTGATGCTTTGGTTCTCAGCTCGTTGGCGTGGTTGGAGGATTTTACCAAGGCACAACAAGTAAAGAAGTCTTCCCACTCAAAACTGAGGAAAACCTGGAAACCGGCAGAGTCGGGAGTTTGGAAGTTAAATGTGGATGAGAGTTTCATTCCTAACACAACTCTCGGTGGTGTGGGAGGTGTTTTAAGAGACGGTGCAGGTCAGTTTCAAGTTGCCTTTGCAAAGCCGATACCAGATGTGGCCTATGACAGGCAAGTAGAGCTTCGAGCTGTAGATGAAGGTTTAAACTTGGTGGGCCACCTTACAATTGAGCACAGTAGTGATCGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Fes1

Protein Analysis

157

Amino Acids

17.77

Weight (kDa)

8.83

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 91 - 152 5.2e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 329
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 390
AgsI TTSAA 2 cut(s) 19, 350
AjnI CCWGG 1 cut(s) 236
AleI CACNNNNGTG 1 cut(s) 459
AluBI AGCT 5 cut(s) 73, 143, 162, 403, 410
AluI AGCT 5 cut(s) 73, 143, 162, 403, 410
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 1 cut(s) 324
AoxI GGCC 3 cut(s) 90, 381, 436
AspS9I GGNCC 2 cut(s) 90, 436
AxyI CCTNAGG 1 cut(s) 6
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 456
BciT130I CCWGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 411
BfuAI ACCTGC 1 cut(s) 329
Bme1390I CCNGG 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 90, 436
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 3 cut(s) 18, 55, 136
BpiI GAAGAC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse118I RCCGGY 1 cut(s) 244
Bse21I CCTNAGG 1 cut(s) 6
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 33, 283
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMII CTCAG 3 cut(s) 20, 172, 218
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 3 cut(s) 92, 383, 438
BsiHKAI GWGCWC 1 cut(s) 456
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 324
BsmBI CGTCTC 1 cut(s) 324
BsnI GGCC 3 cut(s) 92, 383, 438
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 1 cut(s) 463
BspANI GGCC 3 cut(s) 92, 383, 438
BspCNI CTCAG 3 cut(s) 19, 171, 219
BspMI ACCTGC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 244
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 4 cut(s) 63, 128, 335, 458
BstDEI CTNAG 3 cut(s) 6, 158, 227
BstENI CCTNNNNNAGG 1 cut(s) 388
BstF5I GGATG 2 cut(s) 33, 283
BstKTI GATC 1 cut(s) 466
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 1 cut(s) 463
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstSFI CTRYAG 1 cut(s) 411
BstV2I GAAGAC 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 6
BsuRI GGCC 3 cut(s) 92, 383, 438
BtsCI GGATG 2 cut(s) 33, 283
BveI ACCTGC 1 cut(s) 329
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 90, 436
Csp6I GTAC 1 cut(s) 129
CviJI RGCY 9 cut(s) 73, 92, 143, 162, 367, 383, 403, 410, 438
CviKI_1 RGCY 9 cut(s) 73, 92, 143, 162, 367, 383, 403, 410, 438
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 3 cut(s) 6, 158, 227
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
DraI TTTAAA 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 189
Eco81I CCTNAGG 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 324
FaiI YATR 2 cut(s) 88, 387
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FokI GGATG 2 cut(s) 40, 290
HaeIII GGCC 3 cut(s) 92, 383, 438
HapII CCGG 1 cut(s) 245
HinfI GANTC 2 cut(s) 82, 251
HpaII CCGG 1 cut(s) 245
Hpy188III TCNNGA 2 cut(s) 76, 255
HpyAV CCTTC 2 cut(s) 49, 413
HpyCH4III ACNGT 4 cut(s) 63, 128, 335, 458
HpyCH4V TGCA 3 cut(s) 46, 338, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 3 cut(s) 6, 158, 227
Kzo9I GATC 1 cut(s) 463
LpnPI CCDG 6 cut(s) 223, 250, 258, 324, 376, 387
LweI GCATC 3 cut(s) 18, 55, 136
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MboII GAAGA 1 cut(s) 204
MfeI CAATTG 1 cut(s) 447
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 2 cut(s) 112, 447
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 4 cut(s) 15, 172, 222, 311
MseI TTAA 3 cut(s) 269, 326, 425
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 238
MssI GTTTAAAC 1 cut(s) 426
MunI CAATTG 1 cut(s) 447
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeII GATC 1 cut(s) 463
OliI CACNNNNGTG 1 cut(s) 459
PfeI GAWTC 1 cut(s) 82
PleI GAGTC 1 cut(s) 259
PmeI GTTTAAAC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspPI GGNCC 2 cut(s) 90, 436
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
RseI CAYNNNNRTG 1 cut(s) 459
SaqAI TTAA 3 cut(s) 269, 326, 425
Sau3AI GATC 1 cut(s) 463
Sau96I GGNCC 2 cut(s) 90, 436
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 1 cut(s) 456
SfaNI GCATC 3 cut(s) 18, 55, 136
SfcI CTRYAG 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 459
Sse9I AATT 2 cut(s) 112, 447
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 4 cut(s) 63, 128, 335, 458
TaqI TCGA 3 cut(s) 33, 406, 466
TasI AATT 2 cut(s) 112, 447
TatI WGTACW 1 cut(s) 128
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 3 cut(s) 269, 326, 425
Tru9I TTAA 3 cut(s) 269, 326, 425
TspDTI ATGAA 2 cut(s) 277, 432
XagI CCTNNNNNAGG 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.