Rroxscaffold_5G00369910

nucleotide exchange factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009449
Physical Location & Seq
Reverse (-)
51636462 .. 51638082
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369910.1

Sequence Viewer

Length: 1185 bp
ATGGAGCTTCGGAGTTCATGGACGGTAGCGTTAACGTTGACGGCGGTGATGGTTACGGCCGTAACGGCAGACCGGAGTAATAACAAGTCGGGAGGTTTATACTGGTCAACGGCGGAAGAGGAGGCTCAGGCTCAGCTTCAGCCTAGGGTTGACGATTCCGCCGTGGACGCCGACTTCAACGCCGACGGAGGCTTCTCCTCGCTCGATTCGATGTTGCAGTGGGCGTTGGGTCACTCCGATCCCGATCAATTGAAAGAAGCGGCGAAAGAGGTGCAGGGATTGAACCCTTCCGACCTGAACAAGCGGCGGCTCGAGATAAAGCAGCTCATGGAGGAGCTCAAAACGCCGTCGGATGCTCAGCTGATGAAGGTGGCGATACTCGACCTGAAGAACTCGTCGTTGTCGCTGGAAGATCGGCACCGGGCTTTGGAGGAGCTGCTGGAGCTTGTTGAGCCGATTGACAATGCAAATGATTTGAACAAGCTTGGAGGACTTGCTGTAGTGATAGGAGAGCTTGATCATTCTGATTCAGAGACGAGGAAAATGGCTGCATGGATTATTGGGAAGGCTAGTCAGAACAATCCACTTGTTCAGAAGCAGGTTTTGGAACTTGGGGCACTGTCAAAGCTAATGAAGATGGTAAAATCGAGCTTTGTGGAAGAAGCCATTAAGGCAATTTATGCTGTTTCAGCCTTGCTGAGGAATAATATAGCTGGTCAGGAAATGTTCTATGAGGAATCTGGGCAAAAACTGCTTCAGGACATTGTGAGTGATTCGAGCATTGGTAACAGACTGCGCATCAGAGCTGTGGTTCTTCTGGGCGATCTTGCAGAGTTTCAATTAGGAAATACGGATAAGGACGAGCGGCCCTTTTTCAGTAATCGCATTTTCTTGAAGTCTGTTGTTGATCTAACATCAGCTGCTGATCTTCACCTACAGGAAAAGGCCCTCATTGCGATTAAGAGTCTTCTGCGACTCAAGACAACTGAAGCTCTGGTGTTTAAGGACTTTTGTGGGCTGGATGCTGCATTGGAGAGAATGAGGCAGCAGCTGCAGGACTTAATAGTAGAGGAATACCACAGAGATTATGCAATGGACGTAGAAAGCCTCCGCAGCGAAGTGCAGCTGATTTTCCAAAGAAAGCTGGAAAAGGTAGTAACTGGACTTGCTGGTTTTGGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Fes1

Protein Analysis

394

Amino Acids

43.78

Weight (kDa)

4.93

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fes1 PF08609 68 - 159 1.2e-17 Nucleotide exchange factor Fes1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 417
AccBSI CCGCTC 1 cut(s) 865
AciI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 865, 1111
AclI AACGTT 1 cut(s) 35
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 4 cut(s) 122, 407, 740, 1008
AcyI GRCGYC 1 cut(s) 168
AfiI CCNNNNNNNGG 2 cut(s) 427, 699
AgsI TTSAA 6 cut(s) 178, 253, 283, 478, 839, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 2 cut(s) 923, 1051
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 3 cut(s) 57, 866, 945
AspA2I CCTAGG 1 cut(s) 143
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 2 cut(s) 867, 946
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 58, 923
AvaI CYCGRG 1 cut(s) 311
AvrII CCTAGG 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 417
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 959
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 698
BccI CCATC 2 cut(s) 43, 631
BceAI ACGGC 7 cut(s) 44, 57, 72, 81, 126, 146, 331
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 2 cut(s) 144, 570
BfmI CTRYAG 3 cut(s) 498, 935, 1052
BfuAI ACCTGC 1 cut(s) 589
BglI GCCNNNNNGGC 2 cut(s) 65, 671
BlnI CCTAGG 1 cut(s) 143
BlpI GCTNAGC 2 cut(s) 132, 357
Bme1390I CCNGG 1 cut(s) 422
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 867, 946
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 3 cut(s) 343, 807, 1012
BpiI GAAGAC 1 cut(s) 959
BpmI CTGGAG 1 cut(s) 461
Bpu10I CCTNAGC 2 cut(s) 126, 698
Bpu1102I GCTNAGC 2 cut(s) 132, 357
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 243
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 143, 162
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 699
Bse1I ACTGG 2 cut(s) 107, 1165
Bse3DI GCAATG 2 cut(s) 951, 1098
Bse8I GATNNNNATC 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 143, 162
BseGI GGATG 2 cut(s) 358, 1027
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 2 cut(s) 427, 699
BseMI GCAATG 2 cut(s) 951, 1098
BseMII CTCAG 4 cut(s) 140, 146, 371, 689
BseNI ACTGG 2 cut(s) 107, 1165
BseRI GAGGAG 4 cut(s) 134, 187, 347, 446
BseSI GKGCMC 1 cut(s) 619
BseX3I CGGCCG 1 cut(s) 57
BsgI GTGCAG 2 cut(s) 293, 1142
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 3 cut(s) 59, 868, 947
BshNI GGYRCC 1 cut(s) 417
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 2 cut(s) 73, 421
BslI CCNNNNNNNGG 2 cut(s) 427, 699
BsmAI GTCTC 1 cut(s) 527
BsmBI CGTCTC 1 cut(s) 527
BsnI GGCC 3 cut(s) 59, 868, 947
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 339, 619
Bsp143I GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
Bsp1720I GCTNAGC 2 cut(s) 132, 357
BspACI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 865, 1111
BspANI GGCC 3 cut(s) 59, 868, 947
BspCNI CTCAG 4 cut(s) 139, 145, 370, 690
BspLI GGNNCC 1 cut(s) 419
BspMAI CTGCAG 1 cut(s) 1056
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 417
BsrBI CCGCTC 1 cut(s) 865
BsrDI GCAATG 2 cut(s) 951, 1098
BsrI ACTGG 2 cut(s) 107, 1165
BssECI CCNNGG 2 cut(s) 143, 162
BssMI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 25, 621
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 3 cut(s) 680, 751, 1051
BstDEI CTNAG 4 cut(s) 126, 132, 357, 698
BstDSI CCRYGG 1 cut(s) 162
BstENI CCTNNNNNAGG 1 cut(s) 697
BstF5I GGATG 2 cut(s) 358, 1027
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 7 cut(s) 241, 247, 415, 520, 826, 910, 928
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
BstMCI CGRYCG 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 3 cut(s) 498, 935, 1052
BstSLI GKGCMC 1 cut(s) 619
BstV2I GAAGAC 1 cut(s) 959
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 3 cut(s) 59, 868, 947
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 358, 1027
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 224, 617
BveI ACCTGC 1 cut(s) 589
CaiI CAGNNNCTG 2 cut(s) 923, 1051
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 867, 946
CseI GACGC 1 cut(s) 176
CviAII CATG 3 cut(s) 18, 328, 552
DdeI CTNAG 4 cut(s) 126, 132, 357, 698
DpnI GATC 7 cut(s) 240, 246, 414, 519, 825, 909, 927
DpnII GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 2 cut(s) 128, 148
Ecl136II GAGCTC 1 cut(s) 337
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 4 cut(s) 122, 407, 740, 1008
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 946
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 143
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 3 cut(s) 21, 331, 555
FaiI YATR 8 cut(s) 19, 100, 329, 553, 681, 710, 732, 1089
FatI CATG 3 cut(s) 17, 327, 551
FbaI TGATCA 1 cut(s) 517
FokI GGATG 2 cut(s) 365, 1034
FriOI GRGCYC 1 cut(s) 339
FspBI CTAG 2 cut(s) 144, 570
FspI TGCGCA 1 cut(s) 797
GlaI GCGC 1 cut(s) 797
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 3 cut(s) 59, 868, 947
HapII CCGG 2 cut(s) 73, 421
HgaI GACGC 1 cut(s) 176
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 3 cut(s) 21, 331, 555
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 4 cut(s) 33, 39, 108, 151
HindII GTYRAC 4 cut(s) 33, 39, 108, 151
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 7 cut(s) 155, 206, 527, 737, 773, 964, 975
HpaI GTTAAC 1 cut(s) 33
HpaII CCGG 2 cut(s) 73, 421
HphI GGTGA 2 cut(s) 58, 923
Hpy166II GTNNAC 5 cut(s) 33, 39, 108, 151, 166
Hpy188I TCNGA 9 cut(s) 12, 238, 292, 352, 526, 532, 576, 594, 803
Hpy188III TCNNGA 7 cut(s) 90, 242, 313, 719, 758, 892, 979
Hpy8I GTNNAC 5 cut(s) 33, 39, 108, 151, 166
Hpy99I CGWCG 3 cut(s) 188, 352, 400
HpyAV CCTTC 3 cut(s) 297, 361, 559
HpyCH4III ACNGT 2 cut(s) 25, 621
HpyCH4IV ACGT 2 cut(s) 35, 1098
HpyCH4V TGCA 9 cut(s) 217, 274, 467, 551, 830, 1028, 1054, 1091, 1123
HpyF3I CTNAG 4 cut(s) 126, 132, 357, 698
HpySE526I ACGT 2 cut(s) 35, 1098
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 3 cut(s) 21, 331, 555
HspAI GCGC 1 cut(s) 796
Ksp22I TGATCA 1 cut(s) 517
KspAI GTTAAC 1 cut(s) 33
Kzo9I GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
LmnI GCTCC 4 cut(s) 4, 334, 433, 442
LweI GCATC 3 cut(s) 343, 807, 1012
MaeI CTAG 2 cut(s) 144, 570
MaeII ACGT 2 cut(s) 35, 1098
MaeIII GTNAC 5 cut(s) 52, 61, 230, 785, 1156
MalI GATC 7 cut(s) 240, 246, 414, 519, 825, 909, 927
MbiI CCGCTC 1 cut(s) 865
MboI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
MboII GAAGA 8 cut(s) 128, 400, 422, 646, 671, 806, 920, 959
MfeI CAATTG 1 cut(s) 248
MhlI GDGCHC 2 cut(s) 339, 619
MluCI AATT 3 cut(s) 248, 675, 839
MlyI GAGTC 2 cut(s) 969, 973
MmeI TCCRAC 2 cut(s) 315, 330
MseI TTAA 5 cut(s) 32, 669, 960, 1002, 1061
MspA1I CMGCKG 4 cut(s) 361, 920, 1051, 1126
MspI CCGG 2 cut(s) 73, 421
MspR9I CCNGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 248
NciI CCSGG 1 cut(s) 422
NdeII GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
NlaIII CATG 3 cut(s) 21, 331, 555
NlaIV GGNNCC 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 230
NsbI TGCGCA 1 cut(s) 797
PaeR7I CTCGAG 1 cut(s) 311
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 206
PfeI GAWTC 5 cut(s) 155, 206, 527, 737, 773
PleI GAGTC 2 cut(s) 969, 972
PpsI GAGTC 2 cut(s) 969, 972
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 2 cut(s) 867, 946
PstI CTGCAG 1 cut(s) 1056
PstNI CAGNNNCTG 2 cut(s) 923, 1051
PvuII CAGCTG 4 cut(s) 361, 920, 1051, 1126
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 5 cut(s) 32, 669, 960, 1002, 1061
Sau3AI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
Sau96I GGNCC 2 cut(s) 867, 946
SchI GAGTC 2 cut(s) 969, 973
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 2 cut(s) 339, 619
SfaNI GCATC 3 cut(s) 343, 807, 1012
SfcI CTRYAG 3 cut(s) 498, 935, 1052
Sfr274I CTCGAG 1 cut(s) 311
SlaI CTCGAG 1 cut(s) 311
SmlI CTYRAG 2 cut(s) 311, 977
SmoI CTYRAG 2 cut(s) 311, 977
Sse9I AATT 3 cut(s) 248, 675, 839
SsiI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 865, 1111
SspMI CTAG 2 cut(s) 144, 570
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 2 cut(s) 25, 621
TaiI ACGT 2 cut(s) 38, 1101
TaqI TCGA 6 cut(s) 204, 209, 312, 381, 647, 776
TasI AATT 3 cut(s) 248, 675, 839
TauI GCSGC 4 cut(s) 263, 307, 310, 868
TfiI GAWTC 5 cut(s) 155, 206, 527, 737, 773
Tru1I TTAA 5 cut(s) 32, 669, 960, 1002, 1061
Tru9I TTAA 5 cut(s) 32, 669, 960, 1002, 1061
TscAI CASTG 2 cut(s) 224, 624
TseFI GTSAC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 6, 380, 647
TspGWI ACGGA 2 cut(s) 201, 866
TspRI CASTG 2 cut(s) 224, 624
XagI CCTNNNNNAGG 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 311
XmaJI CCTAGG 1 cut(s) 143
XspI CTAG 2 cut(s) 144, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.