FvH4_4g22060

Heparan-alpha-glucosaminide N-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003628
Physical Location & Seq
Forward (+)
25010186 .. 25014818
4633 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22060.t2

Sequence Viewer

Length: 1428 bp
ATGGCGCTGTACGAGCCTATCAAAGTTCACGAAGTTGATGAAGAACACGGAATGGCGGATTCCGTGATCATCAACAACACCAACAAGAATGTAAACGATGTTGAGATGGCTGTTCGGATCTGCAAATTCACATCCTCCGCCCCTCCGAACGACGCCGTTTCCGACGCCACCGCATACAGTCACCTGGAGGCAACCGCATTGCCTCTTTCCAAGGTGCGCCACCAGCAGCAGCAGCAACCCCAACAGCAACGGCTCGTTTCTCTCGACGTCTTTCGCGGACTTACGGTTGTGCTGATGATATTGGTGGACGAAGCTGGTGGACTTGTGCCTGCAATTAACCATGCACCCTGGAATGGTTTGACACTTGCAGATTTAGTCATGCCATTCTTCCTCTTTATGGTTGGTGTTTCACTTTCCCTTGTCTACAAGAAATTGTCATGCAGGGCCATTGCAACTAAGAAAGCGTTATTGAGGGGGTTGAAGCTTCTCGCATTAGGCCTGTTTCTTCAAGGTGGATTTTTCCATGGCATCAAGGAACTAACTTTTGGGGTTGATATTGCAAAGATGAGATGGATGGGCATTCTACAGAGAATTGCAATTGGATATTTAGTAGCTGCATTGTGCGAGATTTGGCTGAAGGGTGATGAAACTGTTACTTCAGGATCATCTCTGCTCAGGAAGTATAAACTTCAGTTGGTTATGGCAGTGATGATAACTGTGACATATCTATCGTTGTTATACGGCTTGCACGTTCCTGATTGGGAGTACCAAGTTTCTACTGGACCTTCCTCTGCACCAAAAACATTTTCCGTGAAATGTGGAGTAAGGGGTGACACAGGACCGGCATGTAATGCTGTTGGGATGATTGATCGCAAGTTATTGGGTCTTCAGCATTTATATAGAAGACCAATCTATTCAAGAACAGCGCAATGCAGTATAAACTCCCCAGATTATGGACGATTACCTGCTCATGCTCCGTCTTGGTGCCAAGCCCCTTTTGATCCTGAAGGAGTTTTAAGTTCATTAATGGCCATTGTTACCTGCTTGGTTGGTTTGCACTATGGTCACATCATTAACCATTTTAAAGATCACCGGAACAGGGTTCTACACTGGACTATCTCATCCACATCTCTTGTAGTCCTAGGCCTCGCCTTGGACTTATTTGGTATGCATATAAACAAGGCTCTCTACACATTCAGTTACATGTGTATGACAGCTGGTTCTGCCGGCATTATCTTTGCTGGAATTTACTTAATGGTTGATGTTTGTGGCTACCGGCGTCCAACTATTGTGCTGGAGTGGATGGGGAAGCATGCATTGGCAATTTTTGTTCTTATAGCTTGCAATCTCTTGCCAGTTATTCTCCATGGATTCTATTGGGGCAAGCCTCAGAATAACGTTCTCACCTTAATTGGAATTGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624

Protein Analysis

476

Amino Acids

52.35

Weight (kDa)

9.0

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 84 - 208 9.6e-07 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
Acc36I ACCTGC 2 cut(s) 973, 1049
AccB1I GGYRCC 1 cut(s) 984
AccB7I CCANNNNNTGG 1 cut(s) 953
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 276
AciI CCGC 5 cut(s) 56, 138, 171, 195, 276
AclI AACGTT 1 cut(s) 1398
AclWI GGATC 3 cut(s) 125, 670, 995
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 2 cut(s) 125, 1245
AcuI CTGAAG 5 cut(s) 642, 656, 674, 872, 1026
AcyI GRCGYC 4 cut(s) 153, 165, 267, 1279
AfaI GTAC 2 cut(s) 11, 767
AfiI CCNNNNNNNGG 5 cut(s) 353, 397, 953, 1099, 1153
AflIII ACRYGT 1 cut(s) 1203
AgsI TTSAA 3 cut(s) 481, 509, 918
AjnI CCWGG 2 cut(s) 183, 347
AjuI GAANNNNNNNTTGG 6 cut(s) 528, 560, 790, 822, 1301, 1333
AluBI AGCT 5 cut(s) 314, 484, 614, 1217, 1340
AluI AGCT 5 cut(s) 314, 484, 614, 1217, 1340
AlwI GGATC 3 cut(s) 125, 670, 995
AoxI GGCC 4 cut(s) 444, 496, 1029, 1144
ApeKI GCWGC 4 cut(s) 226, 229, 232, 614
ApoI RAATTY 2 cut(s) 125, 1245
AseI ATTAAT 1 cut(s) 1025
AspA2I CCTAGG 1 cut(s) 1141
AspLEI GCGC 3 cut(s) 7, 219, 928
AspS9I GGNCC 3 cut(s) 444, 782, 839
AsuHPI GGTGA 5 cut(s) 173, 653, 842, 1082, 1396
AvaII GGWCC 2 cut(s) 782, 839
AvrII CCTAGG 1 cut(s) 1141
BalI TGGCCA 1 cut(s) 1031
BanI GGYRCC 1 cut(s) 984
BbsI GAAGAC 2 cut(s) 878, 910
BbvI GCAGC 4 cut(s) 238, 241, 244, 601
BccI CCATC 4 cut(s) 100, 564, 568, 1297
BceAI ACGGC 3 cut(s) 140, 266, 757
BciT130I CCWGG 2 cut(s) 185, 349
BclI TGATCA 1 cut(s) 66
BfaI CTAG 1 cut(s) 1142
BfmI CTRYAG 1 cut(s) 584
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 2 cut(s) 973, 1049
BisI GCNGC 4 cut(s) 227, 230, 233, 615
BlnI CCTAGG 1 cut(s) 1141
BlsI GCNGC 4 cut(s) 228, 231, 234, 616
Bme1390I CCNGG 2 cut(s) 185, 349
Bme18I GGWCC 2 cut(s) 782, 839
BmgT120I GGNCC 3 cut(s) 444, 782, 839
BmiI GGNNCC 1 cut(s) 986
BmrFI CCNGG 2 cut(s) 185, 349
BmsI GCATC 1 cut(s) 537
BpiI GAAGAC 2 cut(s) 878, 910
BpmI CTGGAG 2 cut(s) 206, 1316
Bpu10I CCTNAGC 1 cut(s) 674
BsaHI GRCGYC 4 cut(s) 153, 165, 267, 1279
BsaJI CCNNGG 6 cut(s) 210, 347, 523, 1141, 1152, 1366
BsaWI WCCGGW 1 cut(s) 1092
Bsc4I CCNNNNNNNGG 5 cut(s) 353, 397, 953, 1099, 1153
Bse118I RCCGGY 3 cut(s) 841, 1226, 1275
Bse1I ACTGG 3 cut(s) 784, 1115, 1355
Bse3DI GCAATG 3 cut(s) 197, 447, 935
BseBI CCWGG 2 cut(s) 185, 349
BseDI CCNNGG 6 cut(s) 210, 347, 523, 1141, 1152, 1366
BseGI GGATG 5 cut(s) 131, 579, 867, 1121, 1308
BseLI CCNNNNNNNGG 5 cut(s) 353, 397, 953, 1099, 1153
BseMI GCAATG 3 cut(s) 197, 447, 935
BseMII CTCAG 2 cut(s) 688, 1403
BseNI ACTGG 3 cut(s) 784, 1115, 1355
BseXI GCAGC 4 cut(s) 238, 241, 244, 601
BsgI GTGCAG 1 cut(s) 777
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 4 cut(s) 446, 498, 1031, 1146
BshNI GGYRCC 1 cut(s) 984
BsiSI CCGG 4 cut(s) 842, 1093, 1227, 1276
BslI CCNNNNNNNGG 5 cut(s) 353, 397, 953, 1099, 1153
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 4 cut(s) 446, 498, 1031, 1146
Bsp143I GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
Bsp19I CCATGG 2 cut(s) 523, 1366
BspACI CCGC 5 cut(s) 56, 138, 171, 195, 276
BspANI GGCC 4 cut(s) 446, 498, 1031, 1146
BspCNI CTCAG 2 cut(s) 687, 1402
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 986
BspMI ACCTGC 2 cut(s) 973, 1049
BspPI GGATC 3 cut(s) 125, 670, 995
BspT107I GGYRCC 1 cut(s) 984
BsrDI GCAATG 3 cut(s) 197, 447, 935
BsrFI RCCGGY 3 cut(s) 841, 1226, 1275
BsrI ACTGG 3 cut(s) 784, 1115, 1355
BssAI RCCGGY 3 cut(s) 841, 1226, 1275
BssECI CCNNGG 6 cut(s) 210, 347, 523, 1141, 1152, 1366
BssMI GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
BssNI GRCGYC 4 cut(s) 153, 165, 267, 1279
BssT1I CCWWGG 5 cut(s) 210, 523, 1141, 1152, 1366
Bst2UI CCWGG 2 cut(s) 185, 349
Bst4CI ACNGT 4 cut(s) 179, 286, 652, 718
BstACI GRCGYC 4 cut(s) 153, 165, 267, 1279
BstAPI GCANNNNNTGC 1 cut(s) 851
BstC8I GCNNGC 6 cut(s) 330, 746, 1228, 1314, 1342, 1385
BstDEI CTNAG 3 cut(s) 456, 674, 1389
BstDSI CCRYGG 2 cut(s) 523, 1366
BstF5I GGATG 5 cut(s) 131, 579, 867, 1121, 1308
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 219, 928
BstKTI GATC 6 cut(s) 69, 120, 665, 871, 1003, 1090
BstMBI GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
BstMWI GCNNNNNNNGC 5 cut(s) 13, 223, 232, 851, 1223
BstNI CCWGG 2 cut(s) 185, 349
BstNSI RCATGY 3 cut(s) 849, 1207, 1316
BstSCI CCNGG 2 cut(s) 183, 347
BstSFI CTRYAG 1 cut(s) 584
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 4 cut(s) 238, 241, 244, 601
BstV2I GAAGAC 2 cut(s) 878, 910
BstX2I RGATCY 1 cut(s) 117
BstYI RGATCY 1 cut(s) 117
BsuRI GGCC 4 cut(s) 446, 498, 1031, 1146
BtgI CCRYGG 2 cut(s) 523, 1366
BtsCI GGATG 5 cut(s) 131, 579, 867, 1121, 1308
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 711, 1108
BveI ACCTGC 2 cut(s) 973, 1049
Cac8I GCNNGC 6 cut(s) 330, 746, 1228, 1314, 1342, 1385
CfoI GCGC 3 cut(s) 7, 219, 928
Cfr10I RCCGGY 3 cut(s) 841, 1226, 1275
Cfr13I GGNCC 3 cut(s) 444, 782, 839
CseI GACGC 3 cut(s) 161, 173, 1268
Csp6I GTAC 2 cut(s) 10, 766
CspCI CAANNNNNGTGG 2 cut(s) 1114, 1149
CviAII CATG 9 cut(s) 341, 379, 438, 524, 846, 971, 1204, 1313, 1367
CviQI GTAC 2 cut(s) 10, 766
DdeI CTNAG 3 cut(s) 456, 674, 1389
DpnI GATC 6 cut(s) 68, 119, 664, 870, 1002, 1089
DpnII GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
DraI TTTAAA 1 cut(s) 1084
EaeI YGGCCR 1 cut(s) 1029
EciI GGCGGA 2 cut(s) 71, 127
Eco130I CCWWGG 5 cut(s) 210, 523, 1141, 1152, 1366
Eco147I AGGCCT 2 cut(s) 498, 1146
Eco47I GGWCC 2 cut(s) 782, 839
Eco57I CTGAAG 5 cut(s) 642, 656, 674, 872, 1026
EcoRII CCWGG 2 cut(s) 183, 347
EcoT14I CCWWGG 5 cut(s) 210, 523, 1141, 1152, 1366
EcoT22I ATGCAT 2 cut(s) 1173, 1318
ErhI CCWWGG 5 cut(s) 210, 523, 1141, 1152, 1366
FaeI CATG 9 cut(s) 344, 382, 441, 527, 849, 974, 1207, 1316, 1370
FatI CATG 9 cut(s) 340, 378, 437, 523, 845, 970, 1203, 1312, 1366
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 4 cut(s) 227, 230, 233, 615
FokI GGATG 5 cut(s) 118, 586, 874, 1108, 1315
Fsp4HI GCNGC 4 cut(s) 227, 230, 233, 615
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 3 cut(s) 6, 218, 927
GluI GCNGC 4 cut(s) 227, 230, 233, 615
GsuI CTGGAG 2 cut(s) 206, 1316
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 446, 498, 1031, 1146
HapII CCGG 4 cut(s) 842, 1093, 1227, 1276
HgaI GACGC 3 cut(s) 161, 173, 1268
HhaI GCGC 3 cut(s) 7, 219, 928
Hin1I GRCGYC 4 cut(s) 153, 165, 267, 1279
Hin1II CATG 9 cut(s) 344, 382, 441, 527, 849, 974, 1207, 1316, 1370
Hin6I GCGC 3 cut(s) 5, 217, 926
HinP1I GCGC 3 cut(s) 5, 217, 926
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 59, 1371
HpaII CCGG 4 cut(s) 842, 1093, 1227, 1276
HphI GGTGA 5 cut(s) 173, 653, 842, 1082, 1396
Hpy166II GTNNAC 5 cut(s) 28, 94, 307, 320, 424
Hpy188I TCNGA 4 cut(s) 117, 147, 163, 1392
Hpy188III TCNNGA 7 cut(s) 29, 263, 660, 676, 755, 918, 1004
Hpy8I GTNNAC 5 cut(s) 28, 94, 307, 320, 424
Hpy99I CGWCG 3 cut(s) 155, 167, 269
HpyAV CCTTC 3 cut(s) 631, 795, 1001
HpyCH4III ACNGT 4 cut(s) 179, 286, 652, 718
HpyCH4IV ACGT 3 cut(s) 267, 750, 1398
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 223, 232, 851, 1223
HpyF3I CTNAG 3 cut(s) 456, 674, 1389
HpySE526I ACGT 3 cut(s) 267, 750, 1398
Hsp92I GRCGYC 4 cut(s) 153, 165, 267, 1279
Hsp92II CATG 9 cut(s) 344, 382, 441, 527, 849, 974, 1207, 1316, 1370
HspAI GCGC 3 cut(s) 5, 217, 926
KroI GCCGGC 1 cut(s) 1226
KroNI GCCGGC 1 cut(s) 1228
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
LmnI GCTCC 1 cut(s) 979
Lsp1109I GCAGC 4 cut(s) 238, 241, 244, 601
LweI GCATC 1 cut(s) 537
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 3 cut(s) 267, 750, 1398
MaeIII GTNAC 7 cut(s) 179, 652, 718, 830, 1036, 1064, 1199
MalI GATC 6 cut(s) 68, 119, 664, 870, 1002, 1089
MboI GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
MboII GAAGA 5 cut(s) 53, 379, 497, 878, 915
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 1 cut(s) 117
MlsI TGGCCA 1 cut(s) 1031
MluCI AATT 9 cut(s) 125, 333, 431, 591, 597, 1245, 1323, 1410, 1416
MluNI TGGCCA 1 cut(s) 1031
MmeI TCCRAC 2 cut(s) 186, 1307
MnlI CCTC 9 cut(s) 145, 153, 181, 213, 401, 465, 799, 1157, 1398
Mox20I TGGCCA 1 cut(s) 1031
Mph1103I ATGCAT 2 cut(s) 1173, 1318
MroNI GCCGGC 1 cut(s) 1226
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 7 cut(s) 336, 1016, 1025, 1074, 1083, 1253, 1409
MslI CAYNNNNRTG 1 cut(s) 1208
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 1 cut(s) 1217
MspI CCGG 4 cut(s) 842, 1093, 1227, 1276
MspR9I CCNGG 2 cut(s) 185, 349
MunI CAATTG 1 cut(s) 597
Mva1269I GAATGC 1 cut(s) 579
MvaI CCWGG 2 cut(s) 185, 349
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 5 cut(s) 13, 223, 232, 851, 1223
NaeI GCCGGC 1 cut(s) 1228
NcoI CCATGG 2 cut(s) 523, 1366
NdeII GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
NgoMIV GCCGGC 1 cut(s) 1226
NlaIII CATG 9 cut(s) 344, 382, 441, 527, 849, 974, 1207, 1316, 1370
NlaIV GGNNCC 1 cut(s) 986
NmuCI GTSAC 4 cut(s) 179, 718, 830, 1064
NsiI ATGCAT 2 cut(s) 1173, 1318
NspI RCATGY 3 cut(s) 849, 1207, 1316
PaeI GCATGC 1 cut(s) 1316
PceI AGGCCT 2 cut(s) 498, 1146
PciI ACATGT 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 3 cut(s) 159, 261, 747
PctI GAATGC 1 cut(s) 579
PdiI GCCGGC 1 cut(s) 1228
PfeI GAWTC 2 cut(s) 59, 1371
PflMI CCANNNNNTGG 1 cut(s) 953
PkrI GCNGC 4 cut(s) 228, 231, 234, 616
PscI ACATGT 1 cut(s) 1203
PshBI ATTAAT 1 cut(s) 1025
Psp1406I AACGTT 1 cut(s) 1398
Psp6I CCWGG 2 cut(s) 183, 347
PspGI CCWGG 2 cut(s) 183, 347
PspN4I GGNNCC 1 cut(s) 986
PspPI GGNCC 3 cut(s) 444, 782, 839
PsuI RGATCY 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 1217
RsaI GTAC 2 cut(s) 11, 767
RsaNI GTAC 2 cut(s) 10, 766
RseI CAYNNNNRTG 1 cut(s) 1208
SaqAI TTAA 7 cut(s) 336, 1016, 1025, 1074, 1083, 1253, 1409
SatI GCNGC 4 cut(s) 227, 230, 233, 615
Sau3AI GATC 6 cut(s) 66, 117, 662, 868, 1000, 1087
Sau96I GGNCC 3 cut(s) 444, 782, 839
ScrFI CCNGG 2 cut(s) 185, 349
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 1 cut(s) 584
SinI GGWCC 2 cut(s) 782, 839
SmiMI CAYNNNNRTG 1 cut(s) 1208
SphI GCATGC 1 cut(s) 1316
Sse9I AATT 9 cut(s) 125, 333, 431, 591, 597, 1245, 1323, 1410, 1416
SseBI AGGCCT 2 cut(s) 498, 1146
SsiI CCGC 5 cut(s) 56, 138, 171, 195, 276
SspMI CTAG 1 cut(s) 1142
StuI AGGCCT 2 cut(s) 498, 1146
StyD4I CCNGG 2 cut(s) 183, 347
StyI CCWWGG 5 cut(s) 210, 523, 1141, 1152, 1366
TaaI ACNGT 4 cut(s) 179, 286, 652, 718
TaiI ACGT 3 cut(s) 270, 753, 1401
TaqI TCGA 1 cut(s) 264
TasI AATT 9 cut(s) 125, 333, 431, 591, 597, 1245, 1323, 1410, 1416
TfiI GAWTC 2 cut(s) 59, 1371
Tru1I TTAA 7 cut(s) 336, 1016, 1025, 1074, 1083, 1253, 1409
Tru9I TTAA 7 cut(s) 336, 1016, 1025, 1074, 1083, 1253, 1409
TscAI CASTG 2 cut(s) 711, 1115
TseFI GTSAC 4 cut(s) 179, 718, 830, 1064
TseI GCWGC 4 cut(s) 226, 229, 232, 614
Tsp45I GTSAC 4 cut(s) 179, 718, 830, 1064
TspDTI ATGAA 3 cut(s) 54, 660, 1011
TspGWI ACGGA 4 cut(s) 52, 63, 799, 966
TspRI CASTG 2 cut(s) 711, 1115
Van91I CCANNNNNTGG 1 cut(s) 953
VpaK11BI GGWCC 2 cut(s) 782, 839
VspI ATTAAT 1 cut(s) 1025
XapI RAATTY 2 cut(s) 125, 1245
XceI RCATGY 3 cut(s) 849, 1207, 1316
XcmI CCANNNNNNNNNTGG 1 cut(s) 776
XmaJI CCTAGG 1 cut(s) 1141
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 1 cut(s) 1142
ZraI GACGTC 1 cut(s) 268
Zsp2I ATGCAT 2 cut(s) 1173, 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.