MD16G1130600.v1.1

Heparan-alpha-glucosaminide N-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003628
Physical Location & Seq
Reverse (-)
9987949 .. 9993921
5973 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1130600.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGGCGATGTACGAGCCTATTAAAGTGTACTCGGACGATGAAAAACGTGGTATGGCGGATCGTATCATGGGGAAGAACATTAATATCGGTGGTGGTGATGGTGTTGGTGGCGGTGATGGCGGTGATGGTCATATGGACGCCGATGTTGAGATGGCCGTCCAGCCGATATCAAAGCCTCCCACCGGCGTCGGGGGCGCTGCTTGTAAGAATATTAGTAGCGGAGAAGCAGCGGTGGCGTCATTGCCTTCGAAGTCGCGGCAACAGCAGCAACGCCTTGTTTCCCTTGACGTCTTTCGTGGACTCACGGTTGCGCTGATGATAATAGTGGACGACATGGGTGGAATCCTGCCTGCAATTAACCATTCACCTTGGAATGGTTTAACCCTTGCAGATTTAGTCATGCCATTCTTCCTGTTTATTGTTGGTCTTTCCCTTGCACTTGCCTACAAGAAACCGTCATGCAGAGCTGATGCTACGAAAAAAGCAGTTTTACGGACGCTGAAGCTTCTAGCATTAGGCCTTTTTCTTCAAGGCGGATTTTTCCATCGCGTCAAGGATCTTACCTTCGGGGTTGATATCACAAAGATCAGATGGATGGGCATTCTACAGAGAATTGCGATTGGATATATTGTAGCAGCGTTGTGCGAGATTTGGCTAAAGCGTGATAGTAATGTCAACTCAGGAAGATCGCTGCTCAGGAAATATCGATCTCAATTGGCTGTGGCATTGATAATCACCACGATATACCTGTCATCGTCATACGGATTATATGTTCCTGATTGGGAGTACCAGATTCCTGTCGATTCCTCCTCTGCACCGAAAACATTTTCAGTAAAATGTGGAGTACGGGGTGACACGGGATCGGCTTGCAATGCAGTTGGGATGATTGATCGTACGATATTGGGGATTGAGCATTTATATAAACGACCAGTCTACGCGCGAACGGAGCAATGCAGTGTTAACTCCCCTGATTATGGCCCGTTGCCTGCCGATGCTCCTTCTTGGTGCCAAGCCCCTTTTGATCCTGAAGGACTTCTGAGTTCAATGATGGCCATTGTTACCTGCTTGGTTGGTTTGCACTACGGGCACATCATTGTCCATTTTAAGGATCACCGGGAAAGAATTCTACACTGGTCTATCTCATCCTCGTCTCTTGTAGTCTTAGGCCTCACCCTCGACTTAATTGGCATGCATATTAACAAGGCTCTTTACACATTCAGTTATATGTGTATCACAGCTGGTGCTGCTGGAATTCTCTTCGCTGCAATTTACTTGATGGTCGATGTGTGTGGCTACAGGCGCCCGGCTATTGTGATGGAATGGATGGGAATGCATGCACTGATGATTTTCGTACTCATAGCCTGCAATATATTGCCAGTTGTCCTCTTTGGATTCTATTGGAGGAAGCCTGAGAACAACATTCTTACCTTGATTGGAATCGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624

Protein Analysis

483

Amino Acids

52.56

Weight (kDa)

8.74

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 91 - 215 4.5e-08 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 1070
AccB1I GGYRCC 2 cut(s) 1005, 1299
AccI GTMKAC 1 cut(s) 933
AccII CGCG 4 cut(s) 256, 549, 938, 940
AciI CCGC 7 cut(s) 56, 111, 120, 219, 230, 256, 534
AclWI GGATC 5 cut(s) 66, 564, 868, 1016, 1116
AcoI YGGCCR 2 cut(s) 153, 1050
AcsI RAATTY 2 cut(s) 1122, 1251
AcuI CTGAAG 2 cut(s) 521, 1047
AcyI GRCGYC 5 cut(s) 138, 186, 236, 288, 1300
AfaI GTAC 6 cut(s) 11, 29, 788, 846, 895, 1353
AfiI CCNNNNNNNGG 5 cut(s) 182, 189, 374, 974, 1105
AgsI TTSAA 2 cut(s) 530, 1044
AloI GAACNNNNNNTCC 2 cut(s) 756, 788
AluBI AGCT 3 cut(s) 467, 505, 1238
AluI AGCT 3 cut(s) 467, 505, 1238
Alw26I GTCTC 1 cut(s) 1155
AlwI GGATC 5 cut(s) 66, 564, 868, 1016, 1116
AoxI GGCC 5 cut(s) 153, 517, 976, 1050, 1165
ApeKI GCWGC 7 cut(s) 197, 227, 265, 635, 691, 1244, 1262
ApoI RAATTY 2 cut(s) 1122, 1251
AseI ATTAAT 1 cut(s) 81
Asp700I GAANNNNTTC 2 cut(s) 1032, 1122
AspLEI GCGC 4 cut(s) 197, 313, 940, 1302
AspS9I GGNCC 1 cut(s) 977
AsuC2I CCSGG 2 cut(s) 1115, 1304
AsuHPI GGTGA 8 cut(s) 107, 125, 134, 357, 727, 863, 1103, 1162
AsuII TTCGAA 1 cut(s) 248
BaeGI GKGCMC 1 cut(s) 1089
BalI TGGCCA 1 cut(s) 1052
BanI GGYRCC 2 cut(s) 1005, 1299
BbvI GCAGC 7 cut(s) 184, 239, 277, 647, 678, 1231, 1249
BceAI ACGGC 1 cut(s) 140
BcnI CCSGG 2 cut(s) 1115, 1304
BcoDI GTCTC 1 cut(s) 1155
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 2 cut(s) 605, 1294
BfoI RGCGCY 2 cut(s) 198, 1303
BfuAI ACCTGC 1 cut(s) 1070
BisI GCNGC 8 cut(s) 198, 228, 257, 266, 636, 692, 1245, 1263
BlsI GCNGC 8 cut(s) 199, 229, 258, 267, 637, 693, 1246, 1264
Bme1390I CCNGG 2 cut(s) 1115, 1304
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 2 cut(s) 1007, 1301
BmrFI CCNGG 2 cut(s) 1115, 1304
BmsI GCATC 2 cut(s) 460, 982
Bpu10I CCTNAGC 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 1115, 1304
Bsa29I ATCGAT 1 cut(s) 706
BsaBI GATNNNNATC 1 cut(s) 1436
BsaHI GRCGYC 5 cut(s) 138, 186, 236, 288, 1300
BsaJI CCNNGG 1 cut(s) 368
Bsc4I CCNNNNNNNGG 5 cut(s) 182, 189, 374, 974, 1105
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 929, 1136, 1376
Bse3DI GCAATG 3 cut(s) 239, 877, 956
Bse8I GATNNNNATC 1 cut(s) 1436
BseCI ATCGAT 1 cut(s) 706
BseDI CCNNGG 1 cut(s) 368
BseGI GGATG 4 cut(s) 600, 888, 1142, 1329
BseJI GATNNNNATC 1 cut(s) 1436
BseLI CCNNNNNNNGG 5 cut(s) 182, 189, 374, 974, 1105
BseMI GCAATG 3 cut(s) 239, 877, 956
BseMII CTCAG 4 cut(s) 693, 709, 1028, 1401
BseNI ACTGG 3 cut(s) 929, 1136, 1376
BseRI GAGGAG 1 cut(s) 799
BseSI GKGCMC 1 cut(s) 1089
BseXI GCAGC 7 cut(s) 184, 239, 277, 647, 678, 1231, 1249
BsgI GTGCAG 1 cut(s) 798
Bsh1236I CGCG 4 cut(s) 256, 549, 938, 940
BshFI GGCC 5 cut(s) 155, 519, 978, 1052, 1167
BshNI GGYRCC 2 cut(s) 1005, 1299
BshVI ATCGAT 1 cut(s) 706
BsiSI CCGG 3 cut(s) 183, 1114, 1304
BsiWI CGTACG 1 cut(s) 893
BslI CCNNNNNNNGG 5 cut(s) 182, 189, 374, 974, 1105
BsmAI GTCTC 1 cut(s) 1155
BsmBI CGTCTC 1 cut(s) 1155
BsmI GAATGC 2 cut(s) 600, 1335
BsnI GGCC 5 cut(s) 155, 519, 978, 1052, 1167
Bsp119I TTCGAA 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 1089
Bsp143I GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
BspACI CCGC 7 cut(s) 56, 111, 120, 219, 230, 256, 534
BspANI GGCC 5 cut(s) 155, 519, 978, 1052, 1167
BspCNI CTCAG 4 cut(s) 692, 708, 1029, 1402
BspDI ATCGAT 1 cut(s) 706
BspFNI CGCG 4 cut(s) 256, 549, 938, 940
BspLI GGNNCC 2 cut(s) 1007, 1301
BspMI ACCTGC 1 cut(s) 1070
BspPI GGATC 5 cut(s) 66, 564, 868, 1016, 1116
BspT104I TTCGAA 1 cut(s) 248
BspT107I GGYRCC 2 cut(s) 1005, 1299
BsrDI GCAATG 3 cut(s) 239, 877, 956
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 929, 1136, 1376
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
BssNI GRCGYC 5 cut(s) 138, 186, 236, 288, 1300
BssT1I CCWWGG 1 cut(s) 368
Bst4CI ACNGT 2 cut(s) 307, 456
Bst6I CTCTTC 1 cut(s) 1262
BstACI GRCGYC 5 cut(s) 138, 186, 236, 288, 1300
BstBI TTCGAA 1 cut(s) 248
BstC8I GCNNGC 6 cut(s) 351, 868, 987, 1190, 1335, 1363
BstDEI CTNAG 5 cut(s) 679, 695, 1037, 1162, 1410
BstF5I GGATG 4 cut(s) 600, 888, 1142, 1329
BstFNI CGCG 4 cut(s) 256, 549, 938, 940
BstH2I RGCGCY 2 cut(s) 198, 1303
BstHHI GCGC 4 cut(s) 197, 313, 940, 1302
BstKTI GATC 9 cut(s) 61, 559, 588, 689, 710, 863, 892, 1024, 1111
BstMAI GTCTC 1 cut(s) 1155
BstMBI GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
BstNSI RCATGY 2 cut(s) 1192, 1337
BstSCI CCNGG 2 cut(s) 1113, 1302
BstSFI CTRYAG 2 cut(s) 605, 1294
BstSLI GKGCMC 1 cut(s) 1089
BstUI CGCG 4 cut(s) 256, 549, 938, 940
BstV1I GCAGC 7 cut(s) 184, 239, 277, 647, 678, 1231, 1249
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
Bsu15I ATCGAT 1 cut(s) 706
BsuRI GGCC 5 cut(s) 155, 519, 978, 1052, 1167
BsuTUI ATCGAT 1 cut(s) 706
BtgZI GCGATG 2 cut(s) 20, 530
BtsCI GGATG 4 cut(s) 600, 888, 1142, 1329
BtsI GCAGTG 1 cut(s) 961
BtsIMutI CAGTG 3 cut(s) 961, 1129, 1337
BveI ACCTGC 1 cut(s) 1070
Cac8I GCNNGC 6 cut(s) 351, 868, 987, 1190, 1335, 1363
CfoI GCGC 4 cut(s) 197, 313, 940, 1302
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 977
ClaI ATCGAT 1 cut(s) 706
CseI GACGC 5 cut(s) 146, 175, 225, 505, 538
Csp6I GTAC 6 cut(s) 10, 28, 787, 845, 894, 1352
CviAII CATG 6 cut(s) 67, 334, 400, 459, 1189, 1334
CviQI GTAC 6 cut(s) 10, 28, 787, 845, 894, 1352
DdeI CTNAG 5 cut(s) 679, 695, 1037, 1162, 1410
DinI GGCGCC 1 cut(s) 1301
DpnI GATC 9 cut(s) 60, 558, 587, 688, 709, 862, 891, 1023, 1110
DpnII GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
EaeI YGGCCR 2 cut(s) 153, 1050
Eam1104I CTCTTC 1 cut(s) 1262
EarI CTCTTC 1 cut(s) 1262
EciI GGCGGA 2 cut(s) 71, 549
Eco130I CCWWGG 1 cut(s) 368
Eco147I AGGCCT 2 cut(s) 519, 1167
Eco32I GATATC 2 cut(s) 168, 577
Eco57I CTGAAG 2 cut(s) 521, 1047
EcoRI GAATTC 2 cut(s) 1122, 1251
EcoRV GATATC 2 cut(s) 168, 577
EcoT14I CCWWGG 1 cut(s) 368
EcoT22I ATGCAT 2 cut(s) 1194, 1335
EgeI GGCGCC 1 cut(s) 1301
EheI GGCGCC 1 cut(s) 1301
ErhI CCWWGG 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 1155
FaeI CATG 6 cut(s) 70, 337, 403, 462, 1192, 1337
FatI CATG 6 cut(s) 66, 333, 399, 458, 1188, 1333
FauNDI CATATG 1 cut(s) 132
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 8 cut(s) 198, 228, 257, 266, 636, 692, 1245, 1263
FokI GGATG 4 cut(s) 607, 895, 1129, 1336
Fsp4HI GCNGC 8 cut(s) 198, 228, 257, 266, 636, 692, 1245, 1263
FspBI CTAG 1 cut(s) 509
GlaI GCGC 4 cut(s) 196, 312, 939, 1301
GluI GCNGC 8 cut(s) 198, 228, 257, 266, 636, 692, 1245, 1263
HaeII RGCGCY 2 cut(s) 198, 1303
HaeIII GGCC 5 cut(s) 155, 519, 978, 1052, 1167
HapII CCGG 3 cut(s) 183, 1114, 1304
HgaI GACGC 5 cut(s) 146, 175, 225, 505, 538
HhaI GCGC 4 cut(s) 197, 313, 940, 1302
Hin1I GRCGYC 5 cut(s) 138, 186, 236, 288, 1300
Hin1II CATG 6 cut(s) 70, 337, 403, 462, 1192, 1337
Hin6I GCGC 4 cut(s) 195, 311, 938, 1300
HinP1I GCGC 4 cut(s) 195, 311, 938, 1300
HincII GTYRAC 2 cut(s) 676, 961
HindII GTYRAC 2 cut(s) 676, 961
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 6 cut(s) 300, 342, 793, 803, 1392, 1437
HpaI GTTAAC 1 cut(s) 961
HpaII CCGG 3 cut(s) 183, 1114, 1304
HphI GGTGA 8 cut(s) 107, 125, 134, 357, 727, 863, 1103, 1162
Hpy166II GTNNAC 6 cut(s) 28, 299, 328, 676, 934, 961
Hpy188I TCNGA 4 cut(s) 34, 590, 1038, 1442
Hpy188III TCNNGA 4 cut(s) 681, 697, 776, 1025
Hpy8I GTNNAC 6 cut(s) 28, 299, 328, 676, 934, 961
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 4 cut(s) 255, 574, 1008, 1022
HpyCH4III ACNGT 2 cut(s) 307, 456
HpyCH4IV ACGT 2 cut(s) 46, 288
HpyF3I CTNAG 5 cut(s) 679, 695, 1037, 1162, 1410
HpySE526I ACGT 2 cut(s) 46, 288
Hsp92I GRCGYC 5 cut(s) 138, 186, 236, 288, 1300
Hsp92II CATG 6 cut(s) 70, 337, 403, 462, 1192, 1337
HspAI GCGC 4 cut(s) 195, 311, 938, 1300
KasI GGCGCC 1 cut(s) 1299
KspAI GTTAAC 1 cut(s) 961
Kzo9I GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
LmnI GCTCC 2 cut(s) 946, 1000
Lsp1109I GCAGC 7 cut(s) 184, 239, 277, 647, 678, 1231, 1249
LweI GCATC 2 cut(s) 460, 982
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 46, 288
MaeIII GTNAC 2 cut(s) 851, 1057
MalI GATC 9 cut(s) 60, 558, 587, 688, 709, 862, 891, 1023, 1110
MboI GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
MboII GAAGA 5 cut(s) 85, 400, 518, 696, 1249
MfeI CAATTG 1 cut(s) 713
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 1 cut(s) 1089
MlsI TGGCCA 1 cut(s) 1052
MluCI AATT 7 cut(s) 354, 612, 713, 1122, 1182, 1251, 1266
MluNI TGGCCA 1 cut(s) 1052
Mly113I GGCGCC 1 cut(s) 1300
MlyI GAGTC 1 cut(s) 294
MnlI CCTC 8 cut(s) 186, 817, 820, 1156, 1178, 1184, 1394, 1395
Mox20I TGGCCA 1 cut(s) 1052
Mph1103I ATGCAT 2 cut(s) 1194, 1335
MroXI GAANNNNTTC 2 cut(s) 1032, 1122
MscI TGGCCA 1 cut(s) 1052
MseI TTAA 8 cut(s) 21, 81, 357, 380, 960, 1104, 1181, 1197
Msp20I TGGCCA 1 cut(s) 1052
MspA1I CMGCKG 2 cut(s) 230, 1238
MspI CCGG 3 cut(s) 183, 1114, 1304
MspR9I CCNGG 2 cut(s) 1115, 1304
MunI CAATTG 1 cut(s) 713
Mva1269I GAATGC 2 cut(s) 600, 1335
MvnI CGCG 4 cut(s) 256, 549, 938, 940
NarI GGCGCC 1 cut(s) 1300
NciI CCSGG 2 cut(s) 1115, 1304
NdeI CATATG 1 cut(s) 132
NdeII GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
NlaIII CATG 6 cut(s) 70, 337, 403, 462, 1192, 1337
NlaIV GGNNCC 2 cut(s) 1007, 1301
NmuCI GTSAC 1 cut(s) 851
NsiI ATGCAT 2 cut(s) 1194, 1335
NspI RCATGY 2 cut(s) 1192, 1337
NspV TTCGAA 1 cut(s) 248
PaeI GCATGC 2 cut(s) 1192, 1337
PceI AGGCCT 2 cut(s) 519, 1167
PctI GAATGC 2 cut(s) 600, 1335
PdmI GAANNNNTTC 2 cut(s) 1032, 1122
PfeI GAWTC 5 cut(s) 342, 793, 803, 1392, 1437
Pfl23II CGTACG 1 cut(s) 893
PkrI GCNGC 8 cut(s) 199, 229, 258, 267, 637, 693, 1246, 1264
PleI GAGTC 1 cut(s) 294
PluTI GGCGCC 1 cut(s) 1303
PpsI GAGTC 1 cut(s) 294
PshBI ATTAAT 1 cut(s) 81
PspLI CGTACG 1 cut(s) 893
PspN4I GGNNCC 2 cut(s) 1007, 1301
PspPI GGNCC 1 cut(s) 977
PsuI RGATCY 1 cut(s) 556
PvuII CAGCTG 1 cut(s) 1238
RsaI GTAC 6 cut(s) 11, 29, 788, 846, 895, 1353
RsaNI GTAC 6 cut(s) 10, 28, 787, 845, 894, 1352
SaqAI TTAA 8 cut(s) 21, 81, 357, 380, 960, 1104, 1181, 1197
SatI GCNGC 8 cut(s) 198, 228, 257, 266, 636, 692, 1245, 1263
Sau3AI GATC 9 cut(s) 58, 556, 585, 686, 707, 860, 889, 1021, 1108
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 2 cut(s) 1115, 1304
SduI GDGCHC 1 cut(s) 1089
SfaNI GCATC 2 cut(s) 460, 982
SfcI CTRYAG 2 cut(s) 605, 1294
SfoI GGCGCC 1 cut(s) 1301
SfuI TTCGAA 1 cut(s) 248
SgrAI CRCCGGYG 1 cut(s) 182
SphI GCATGC 2 cut(s) 1192, 1337
Sse9I AATT 7 cut(s) 354, 612, 713, 1122, 1182, 1251, 1266
SseBI AGGCCT 2 cut(s) 519, 1167
SsiI CCGC 7 cut(s) 56, 111, 120, 219, 230, 256, 534
SspDI GGCGCC 1 cut(s) 1299
SspI AATATT 1 cut(s) 211
SspMI CTAG 1 cut(s) 509
StuI AGGCCT 2 cut(s) 519, 1167
StyD4I CCNGG 2 cut(s) 1113, 1302
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 2 cut(s) 307, 456
TaiI ACGT 2 cut(s) 49, 291
TaqI TCGA 5 cut(s) 248, 706, 801, 1176, 1281
TasI AATT 7 cut(s) 354, 612, 713, 1122, 1182, 1251, 1266
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 5 cut(s) 342, 793, 803, 1392, 1437
Tru1I TTAA 8 cut(s) 21, 81, 357, 380, 960, 1104, 1181, 1197
Tru9I TTAA 8 cut(s) 21, 81, 357, 380, 960, 1104, 1181, 1197
TscAI CASTG 3 cut(s) 961, 1136, 1344
TseFI GTSAC 1 cut(s) 851
TseI GCWGC 7 cut(s) 197, 227, 265, 635, 691, 1244, 1262
Tsp45I GTSAC 1 cut(s) 851
TspDTI ATGAA 1 cut(s) 54
TspGWI ACGGA 3 cut(s) 508, 777, 959
TspRI CASTG 3 cut(s) 961, 1136, 1344
VspI ATTAAT 1 cut(s) 81
XapI RAATTY 2 cut(s) 1122, 1251
XceI RCATGY 2 cut(s) 1192, 1337
XmiI GTMKAC 1 cut(s) 933
XmnI GAANNNNTTC 2 cut(s) 1032, 1122
XspI CTAG 1 cut(s) 509
ZraI GACGTC 1 cut(s) 289
Zsp2I ATGCAT 2 cut(s) 1194, 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.