RLG00000007166

Heparan-alpha-glucosaminide N-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003628
Physical Location & Seq
Reverse (-)
14180822 .. 14186017
5196 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007166

Sequence Viewer

Length: 1749 bp
ATGACATTTCGAATTCGAGCGGAGCTCCGCTTTGGAGCAGATGATGGTGACGGAGCCGACGATGGAATCGCCGTAAAGGTACCGGAGAGGCGAAAGCTTCTGGAGCAAGAGGTCGTCGCCGTTCGAGTAGCTGAAGCCCCGGGTCTTCTTGTTCCGGTTGCGAATCGGAGTCGTCGGGGAAGCATAATGGGAGTCCAATTGCCAATCAGAGTCGCCAGTTTCCCATGGATCAATTGCTTTATGCGCCGCAGCGAAGGAGAGTCGAGGACGCCTTCATCCATCTCTCTCTTCTCCTTCGCTCCTTCTGCCCCCTCCCTAACCAGGGAGATGGCGCTGTACGAGCCTATCAAAGGTCACGAAGTTGATGAGGAACACAGAATGGCGGATTCCGCGATCCTCAACAACAGTAGCAAGAACGTCGACGATGATGTTGAGATGGCTATCCGGGTCTCCAAACTGAGATCCGCCGCCCCTCCCAACGACGCCGTTTCCGTCGCCACCGCCTACAGTCACCCCGAGGCAACGTCATTGCCCAGTTCTAAGGTGCGCCACCAACAACACCATCAGCAACGGCTCGTTTCGCTCGACGTCTTTCGCGGCCTTACGGTTGTGCTGATGATACTGGTGGACGAAGTTGGTGGACTTGTGCCTGCAATCAACCATGCACCCTGGAATGGTTTGACACTTGCAGATTTAGTCATGCCATTCTTCCTCTTTATGGTTGGTGTTTCACTTTCCCTTGTCTACAAGAAATTATCATGCAGGGCCATTGCAACTAAAAAAGCATTAATAAGGGGGTTGAAGCTTCTAGCATTAGGCCTGTTTCTTCAAGGTGGATTTTTCCACGGCATCAAGGAACTAACTTTCGGGGTCGATATTGCAAAGATGAGATGGATGGGCATTCTCCAGAGAATTGCAATAGGATATTTAGTAGCTGCATTGTGTGAGATTTGGCTAAAGGGTGATGGTACTGTTAGTTCAGGATCAGCTCTGCTCAAGAAGTATAAACTTCAGTTGGTTATGGCACTGATGATGACTGTGACATATCTATCATTATTATACGGCTTGCATGTTCCTGATTGGGAGTACCAAATTTCTACCGGACCCTCCTCTGCACCAAAAACATTTTCGGTGAAATGTGGAGTAAGGGGTGACACAGGACCGGCATGTAATGCTGTTGGGATGATTGATCGCAAGTTATTGGGCCTTCAGCATTTATATAGAAGACCAATCTATTCAAGAACAGTGCAATGCAGTATTAACTCCCCGGATTATGGGCCATTACCTGCTGATGCTCCTTCTTGGTGCCAAGCCCCTTTTGATCCTGAAGGACTTTTAAGTTCACTGATGGCCATTGTTACCTGCTTGGTTGGTTTGCACTATGGTCACATCATTAACCATTTTAAAGATCACCGGAACAGAGTTCTGCACTGGACTATCTCATCCACGTCTCTCGTAGTCTTAGGCCTCGCCTTGGACTTATTTGGTATGCATATAAACAAGGCTCTCTACACATTCAGTTACATGTCTATGACAGCTGGTTCTGCCGGCATTATCTTTGCTGGGATTTACTTAATGGTTGATGTTTGTGGCTACCGGCGTCCAACTATTTTGTTGGAGTGGATGGGGAAGCACGCATTGGCAATTTTTATTCTTGTAGCTTGCAATCTCTTGCCAGTTATTCTCCATGGATTCTATTGGAGCAAGCCTCAGAATAACATTCTCACCTTAATTGGAATTGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624

Protein Analysis

583

Amino Acids

63.97

Weight (kDa)

9.21

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 191 - 315 4.5e-07 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 591
Acc36I ACCTGC 2 cut(s) 1294, 1370
Acc65I GGTACC 1 cut(s) 79
AccB1I GGYRCC 2 cut(s) 79, 1305
AccBSI CCGCTC 1 cut(s) 20
AccI GTMKAC 2 cut(s) 420, 744
AccII CGCG 2 cut(s) 392, 597
AciI CCGC 9 cut(s) 20, 28, 247, 383, 390, 465, 468, 501, 597
AclWI GGATC 5 cut(s) 236, 388, 456, 991, 1316
AcoI YGGCCR 1 cut(s) 1350
AcsI RAATTY 2 cut(s) 12, 1092
AcuI CTGAAG 4 cut(s) 153, 995, 1193, 1347
AcyI GRCGYC 4 cut(s) 269, 483, 588, 1600
AfaI GTAC 4 cut(s) 81, 338, 970, 1088
AfiI CCNNNNNNNGG 7 cut(s) 321, 322, 350, 674, 718, 1274, 1474
AflIII ACRYGT 1 cut(s) 1524
AgsI TTSAA 3 cut(s) 802, 830, 1239
AjiI CACGTC 1 cut(s) 1449
AjnI CCWGG 2 cut(s) 320, 668
AjuI GAANNNNNNNTTGG 4 cut(s) 1111, 1143, 1622, 1654
AluBI AGCT 8 cut(s) 25, 97, 131, 805, 935, 989, 1538, 1661
AluI AGCT 8 cut(s) 25, 97, 131, 805, 935, 989, 1538, 1661
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 454, 1455
AlwI GGATC 5 cut(s) 236, 388, 456, 991, 1316
Ama87I CYCGRG 2 cut(s) 139, 515
AoxI GGCC 7 cut(s) 598, 765, 817, 1204, 1277, 1350, 1465
ApeKI GCWGC 2 cut(s) 249, 935
ApoI RAATTY 2 cut(s) 12, 1092
AseI ATTAAT 1 cut(s) 788
Asp718I GGTACC 1 cut(s) 79
AspLEI GCGC 3 cut(s) 246, 334, 549
AspS9I GGNCC 5 cut(s) 765, 1103, 1160, 1204, 1277
AsuC2I CCSGG 4 cut(s) 140, 141, 446, 1268
AsuHPI GGTGA 7 cut(s) 59, 503, 974, 1144, 1163, 1403, 1717
AsuII TTCGAA 1 cut(s) 10
AvaI CYCGRG 2 cut(s) 139, 515
AvaII GGWCC 2 cut(s) 1103, 1160
BalI TGGCCA 1 cut(s) 1352
BanI GGYRCC 2 cut(s) 79, 1305
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 2 cut(s) 137, 1231
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 2 cut(s) 261, 922
BceAI ACGGC 6 cut(s) 56, 104, 470, 587, 862, 1078
BcgI CGANNNNNNTGC 2 cut(s) 400, 434
BciT130I CCWGG 2 cut(s) 322, 670
BcnI CCSGG 4 cut(s) 140, 141, 446, 1268
BcoDI GTCTC 2 cut(s) 454, 1455
BfaI CTAG 1 cut(s) 809
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 335
BfuAI ACCTGC 2 cut(s) 1294, 1370
BisI GCNGC 5 cut(s) 247, 250, 468, 598, 936
BlsI GCNGC 5 cut(s) 248, 251, 469, 599, 937
Bme1390I CCNGG 6 cut(s) 140, 141, 322, 446, 670, 1268
Bme18I GGWCC 2 cut(s) 1103, 1160
BmeT110I CYCGRG 2 cut(s) 139, 515
BmgBI CACGTC 1 cut(s) 1449
BmgT120I GGNCC 5 cut(s) 765, 1103, 1160, 1204, 1277
BmiI GGNNCC 4 cut(s) 55, 81, 1105, 1307
BmrFI CCNGG 6 cut(s) 140, 141, 322, 446, 670, 1268
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 2 cut(s) 858, 1282
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 2 cut(s) 137, 1231
BplI GAGNNNNNCTC 4 cut(s) 9, 41, 1693, 1725
BpmI CTGGAG 2 cut(s) 122, 890
Bpu14I TTCGAA 1 cut(s) 10
BpuEI CTTGAG 1 cut(s) 980
BpuMI CCSGG 4 cut(s) 140, 141, 446, 1268
BsaBI GATNNNNATC 1 cut(s) 440
BsaHI GRCGYC 4 cut(s) 269, 483, 588, 1600
BsaI GGTCTC 1 cut(s) 454
BsaWI WCCGGW 4 cut(s) 82, 154, 1100, 1413
Bsc4I CCNNNNNNNGG 7 cut(s) 321, 322, 350, 674, 718, 1274, 1474
Bse118I RCCGGY 3 cut(s) 1162, 1547, 1596
Bse1I ACTGG 5 cut(s) 216, 534, 627, 1436, 1676
Bse3DI GCAATG 3 cut(s) 527, 768, 1256
Bse8I GATNNNNATC 1 cut(s) 440
BseBI CCWGG 2 cut(s) 322, 670
BseGI GGATG 5 cut(s) 275, 900, 1188, 1442, 1629
BseJI GATNNNNATC 1 cut(s) 440
BseLI CCNNNNNNNGG 7 cut(s) 321, 322, 350, 674, 718, 1274, 1474
BseMI GCAATG 3 cut(s) 527, 768, 1256
BseMII CTCAG 2 cut(s) 449, 1724
BseNI ACTGG 5 cut(s) 216, 534, 627, 1436, 1676
BseRI GAGGAG 1 cut(s) 1099
BseXI GCAGC 2 cut(s) 261, 922
BseYI CCCAGC 1 cut(s) 1562
BsgI GTGCAG 2 cut(s) 1098, 1412
Bsh1236I CGCG 2 cut(s) 392, 597
BshFI GGCC 7 cut(s) 600, 767, 819, 1206, 1279, 1352, 1467
BshNI GGYRCC 2 cut(s) 79, 1305
BsiHKAI GWGCWC 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 139, 515
BslI CCNNNNNNNGG 7 cut(s) 321, 322, 350, 674, 718, 1274, 1474
BsmAI GTCTC 2 cut(s) 454, 1455
BsmBI CGTCTC 1 cut(s) 1455
BsmI GAATGC 1 cut(s) 900
BsnI GGCC 7 cut(s) 600, 767, 819, 1206, 1279, 1352, 1467
Bso31I GGTCTC 1 cut(s) 454
BsoBI CYCGRG 2 cut(s) 139, 515
Bsp119I TTCGAA 1 cut(s) 10
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
Bsp19I CCATGG 2 cut(s) 224, 1687
BspACI CCGC 9 cut(s) 20, 28, 247, 383, 390, 465, 468, 501, 597
BspANI GGCC 7 cut(s) 600, 767, 819, 1206, 1279, 1352, 1467
BspCNI CTCAG 2 cut(s) 450, 1723
BspFNI CGCG 2 cut(s) 392, 597
BspLI GGNNCC 4 cut(s) 55, 81, 1105, 1307
BspMI ACCTGC 2 cut(s) 1294, 1370
BspPI GGATC 5 cut(s) 236, 388, 456, 991, 1316
BspT104I TTCGAA 1 cut(s) 10
BspT107I GGYRCC 2 cut(s) 79, 1305
BspTNI GGTCTC 1 cut(s) 454
BsrBI CCGCTC 1 cut(s) 20
BsrDI GCAATG 3 cut(s) 527, 768, 1256
BsrFI RCCGGY 3 cut(s) 1162, 1547, 1596
BsrI ACTGG 5 cut(s) 216, 534, 627, 1436, 1676
BssAI RCCGGY 3 cut(s) 1162, 1547, 1596
BssMI GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
BssNI GRCGYC 4 cut(s) 269, 483, 588, 1600
BssT1I CCWWGG 3 cut(s) 224, 1473, 1687
Bst2UI CCWGG 2 cut(s) 322, 670
Bst4CI ACNGT 6 cut(s) 407, 509, 607, 973, 1039, 1246
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 4 cut(s) 269, 483, 588, 1600
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstBI TTCGAA 1 cut(s) 10
BstC8I GCNNGC 6 cut(s) 651, 1067, 1549, 1635, 1663, 1706
BstDEI CTNAG 4 cut(s) 458, 540, 1462, 1710
BstDSI CCRYGG 3 cut(s) 224, 844, 1687
BstENI CCTNNNNNAGG 1 cut(s) 348
BstF5I GGATG 5 cut(s) 275, 900, 1188, 1442, 1629
BstFNI CGCG 2 cut(s) 392, 597
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 3 cut(s) 246, 334, 549
BstKTI GATC 7 cut(s) 231, 396, 464, 986, 1192, 1324, 1411
BstMAI GTCTC 2 cut(s) 454, 1455
BstMBI GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
BstMWI GCNNNNNNNGC 8 cut(s) 103, 243, 305, 340, 389, 580, 1172, 1544
BstNI CCWGG 2 cut(s) 322, 670
BstNSI RCATGY 3 cut(s) 1073, 1170, 1528
BstSCI CCNGG 6 cut(s) 138, 139, 320, 444, 668, 1266
BstSFI CTRYAG 1 cut(s) 505
BstUI CGCG 2 cut(s) 392, 597
BstV1I GCAGC 2 cut(s) 261, 922
BstV2I GAAGAC 2 cut(s) 137, 1231
BstX2I RGATCY 1 cut(s) 461
BstXI CCANNNNNNTGG 1 cut(s) 328
BstYI RGATCY 1 cut(s) 461
BsuRI GGCC 7 cut(s) 600, 767, 819, 1206, 1279, 1352, 1467
BtgI CCRYGG 3 cut(s) 224, 844, 1687
BtrI CACGTC 1 cut(s) 1449
BtsCI GGATG 5 cut(s) 275, 900, 1188, 1442, 1629
BtsIMutI CAGTG 4 cut(s) 1025, 1251, 1343, 1429
BveI ACCTGC 2 cut(s) 1294, 1370
Cac8I GCNNGC 6 cut(s) 651, 1067, 1549, 1635, 1663, 1706
CfoI GCGC 3 cut(s) 246, 334, 549
Cfr10I RCCGGY 3 cut(s) 1162, 1547, 1596
Cfr13I GGNCC 5 cut(s) 765, 1103, 1160, 1204, 1277
Cfr9I CCCGGG 1 cut(s) 139
CseI GACGC 3 cut(s) 277, 491, 1589
Csp6I GTAC 4 cut(s) 80, 337, 969, 1087
CviAII CATG 8 cut(s) 225, 662, 700, 759, 1070, 1167, 1525, 1688
CviQI GTAC 4 cut(s) 80, 337, 969, 1087
DdeI CTNAG 4 cut(s) 458, 540, 1462, 1710
DpnI GATC 7 cut(s) 230, 395, 463, 985, 1191, 1323, 1410
DpnII GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
DraI TTTAAA 1 cut(s) 1405
EaeI YGGCCR 1 cut(s) 1350
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 2 cut(s) 398, 454
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 3 cut(s) 224, 1473, 1687
Eco147I AGGCCT 2 cut(s) 819, 1467
Eco24I GRGCYC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 454
Eco47I GGWCC 2 cut(s) 1103, 1160
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 4 cut(s) 153, 995, 1193, 1347
Eco88I CYCGRG 2 cut(s) 139, 515
EcoICRI GAGCTC 1 cut(s) 25
EcoNI CCTNNNNNAGG 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 320, 668
EcoT14I CCWWGG 3 cut(s) 224, 1473, 1687
EcoT22I ATGCAT 1 cut(s) 1494
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 3 cut(s) 224, 1473, 1687
Esp3I CGTCTC 1 cut(s) 1455
FaeI CATG 8 cut(s) 228, 665, 703, 762, 1073, 1170, 1528, 1691
FatI CATG 8 cut(s) 224, 661, 699, 758, 1069, 1166, 1524, 1687
FblI GTMKAC 2 cut(s) 420, 744
Fnu4HI GCNGC 5 cut(s) 247, 250, 468, 598, 936
FokI GGATG 5 cut(s) 262, 907, 1195, 1429, 1636
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 5 cut(s) 247, 250, 468, 598, 936
FspBI CTAG 1 cut(s) 809
GlaI GCGC 3 cut(s) 245, 333, 548
GluI GCNGC 5 cut(s) 247, 250, 468, 598, 936
GsaI CCCAGC 1 cut(s) 1566
GsuI CTGGAG 2 cut(s) 122, 890
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 7 cut(s) 600, 767, 819, 1206, 1279, 1352, 1467
HgaI GACGC 3 cut(s) 277, 491, 1589
HhaI GCGC 3 cut(s) 246, 334, 549
Hin1I GRCGYC 4 cut(s) 269, 483, 588, 1600
Hin1II CATG 8 cut(s) 228, 665, 703, 762, 1073, 1170, 1528, 1691
Hin6I GCGC 3 cut(s) 244, 332, 547
HinP1I GCGC 3 cut(s) 244, 332, 547
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 2 cut(s) 95, 803
HinfI GANTC 8 cut(s) 66, 163, 169, 192, 210, 260, 386, 1692
HphI GGTGA 7 cut(s) 59, 503, 974, 1144, 1163, 1403, 1717
Hpy166II GTNNAC 5 cut(s) 421, 628, 641, 745, 1343
Hpy188I TCNGA 3 cut(s) 168, 209, 1713
Hpy188III TCNNGA 8 cut(s) 101, 356, 907, 981, 997, 1076, 1239, 1325
Hpy8I GTNNAC 5 cut(s) 421, 628, 641, 745, 1343
Hpy99I CGWCG 8 cut(s) 62, 119, 177, 422, 425, 485, 497, 590
HpyAV CCTTC 7 cut(s) 248, 282, 304, 312, 1217, 1308, 1322
HpyCH4III ACNGT 6 cut(s) 407, 509, 607, 973, 1039, 1246
HpyCH4IV ACGT 4 cut(s) 417, 524, 588, 1448
HpyF10VI GCNNNNNNNGC 8 cut(s) 103, 243, 305, 340, 389, 580, 1172, 1544
HpyF3I CTNAG 4 cut(s) 458, 540, 1462, 1710
HpySE526I ACGT 4 cut(s) 417, 524, 588, 1448
Hsp92I GRCGYC 4 cut(s) 269, 483, 588, 1600
Hsp92II CATG 8 cut(s) 228, 665, 703, 762, 1073, 1170, 1528, 1691
HspAI GCGC 3 cut(s) 244, 332, 547
KpnI GGTACC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 1547
KroNI GCCGGC 1 cut(s) 1549
Kzo9I GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
LmnI GCTCC 8 cut(s) 22, 30, 35, 53, 103, 304, 1300, 1701
Lsp1109I GCAGC 2 cut(s) 261, 922
LweI GCATC 2 cut(s) 858, 1282
MaeI CTAG 1 cut(s) 809
MaeII ACGT 4 cut(s) 417, 524, 588, 1448
MaeIII GTNAC 8 cut(s) 47, 353, 509, 1039, 1151, 1357, 1385, 1520
MalI GATC 7 cut(s) 230, 395, 463, 985, 1191, 1323, 1410
MbiI CCGCTC 1 cut(s) 20
MboI GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
MboII GAAGA 5 cut(s) 137, 280, 700, 818, 1236
MfeI CAATTG 2 cut(s) 197, 232
MflI RGATCY 1 cut(s) 461
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 1 cut(s) 1352
MluCI AATT 9 cut(s) 12, 197, 232, 752, 912, 1092, 1644, 1731, 1737
MluNI TGGCCA 1 cut(s) 1352
MlyI GAGTC 4 cut(s) 178, 201, 219, 269
MmeI TCCRAC 2 cut(s) 1596, 1628
Mox20I TGGCCA 1 cut(s) 1352
Mph1103I ATGCAT 1 cut(s) 1494
MroNI GCCGGC 1 cut(s) 1547
MscI TGGCCA 1 cut(s) 1352
MseI TTAA 7 cut(s) 788, 1260, 1337, 1395, 1404, 1574, 1730
MslI CAYNNNNRTG 1 cut(s) 1529
Msp20I TGGCCA 1 cut(s) 1352
MspA1I CMGCKG 1 cut(s) 1538
MspR9I CCNGG 6 cut(s) 140, 141, 322, 446, 670, 1268
MunI CAATTG 2 cut(s) 197, 232
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 2 cut(s) 322, 670
MvnI CGCG 2 cut(s) 392, 597
MwoI GCNNNNNNNGC 8 cut(s) 103, 243, 305, 340, 389, 580, 1172, 1544
NaeI GCCGGC 1 cut(s) 1549
NciI CCSGG 4 cut(s) 140, 141, 446, 1268
NcoI CCATGG 2 cut(s) 224, 1687
NdeII GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
NgoMIV GCCGGC 1 cut(s) 1547
NlaIII CATG 8 cut(s) 228, 665, 703, 762, 1073, 1170, 1528, 1691
NlaIV GGNNCC 4 cut(s) 55, 81, 1105, 1307
NmuCI GTSAC 6 cut(s) 47, 353, 509, 1039, 1151, 1385
NsiI ATGCAT 1 cut(s) 1494
NspI RCATGY 3 cut(s) 1073, 1170, 1528
NspV TTCGAA 1 cut(s) 10
PceI AGGCCT 2 cut(s) 819, 1467
PciI ACATGT 1 cut(s) 1524
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 489, 582
PctI GAATGC 1 cut(s) 900
PdiI GCCGGC 1 cut(s) 1549
PfeI GAWTC 4 cut(s) 66, 163, 386, 1692
PkrI GCNGC 5 cut(s) 248, 251, 469, 599, 937
PleI GAGTC 4 cut(s) 177, 200, 218, 268
PpsI GAGTC 4 cut(s) 177, 200, 218, 268
PscI ACATGT 1 cut(s) 1524
PshBI ATTAAT 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 320, 668
PspFI CCCAGC 1 cut(s) 1562
PspGI CCWGG 2 cut(s) 320, 668
PspN4I GGNNCC 4 cut(s) 55, 81, 1105, 1307
PspPI GGNCC 5 cut(s) 765, 1103, 1160, 1204, 1277
PsrI GAACNNNNNNTAC 2 cut(s) 961, 993
PsuI RGATCY 1 cut(s) 461
PvuII CAGCTG 1 cut(s) 1538
RsaI GTAC 4 cut(s) 81, 338, 970, 1088
RsaNI GTAC 4 cut(s) 80, 337, 969, 1087
RseI CAYNNNNRTG 1 cut(s) 1529
SacI GAGCTC 1 cut(s) 27
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 7 cut(s) 788, 1260, 1337, 1395, 1404, 1574, 1730
SatI GCNGC 5 cut(s) 247, 250, 468, 598, 936
Sau3AI GATC 7 cut(s) 228, 393, 461, 983, 1189, 1321, 1408
Sau96I GGNCC 5 cut(s) 765, 1103, 1160, 1204, 1277
SchI GAGTC 4 cut(s) 178, 201, 219, 269
ScrFI CCNGG 6 cut(s) 140, 141, 322, 446, 670, 1268
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 2 cut(s) 858, 1282
SfcI CTRYAG 1 cut(s) 505
SfuI TTCGAA 1 cut(s) 10
SgrDI CGTCGACG 1 cut(s) 419
SinI GGWCC 2 cut(s) 1103, 1160
SmaI CCCGGG 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 1529
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
Sse9I AATT 9 cut(s) 12, 197, 232, 752, 912, 1092, 1644, 1731, 1737
SseBI AGGCCT 2 cut(s) 819, 1467
SsiI CCGC 9 cut(s) 20, 28, 247, 383, 390, 465, 468, 501, 597
SspMI CTAG 1 cut(s) 809
SstI GAGCTC 1 cut(s) 27
StuI AGGCCT 2 cut(s) 819, 1467
StyD4I CCNGG 6 cut(s) 138, 139, 320, 444, 668, 1266
StyI CCWWGG 3 cut(s) 224, 1473, 1687
TaaI ACNGT 6 cut(s) 407, 509, 607, 973, 1039, 1246
TaiI ACGT 4 cut(s) 420, 527, 591, 1451
TaqI TCGA 7 cut(s) 10, 16, 124, 263, 420, 585, 873
TasI AATT 9 cut(s) 12, 197, 232, 752, 912, 1092, 1644, 1731, 1737
TauI GCSGC 3 cut(s) 249, 470, 600
TfiI GAWTC 4 cut(s) 66, 163, 386, 1692
Tru1I TTAA 7 cut(s) 788, 1260, 1337, 1395, 1404, 1574, 1730
Tru9I TTAA 7 cut(s) 788, 1260, 1337, 1395, 1404, 1574, 1730
TscAI CASTG 4 cut(s) 1032, 1251, 1350, 1436
TseFI GTSAC 6 cut(s) 47, 353, 509, 1039, 1151, 1385
TseI GCWGC 2 cut(s) 249, 935
Tsp45I GTSAC 6 cut(s) 47, 353, 509, 1039, 1151, 1385
TspDTI ATGAA 1 cut(s) 264
TspGWI ACGGA 2 cut(s) 66, 481
TspMI CCCGGG 1 cut(s) 139
TspRI CASTG 4 cut(s) 1032, 1251, 1350, 1436
VpaK11BI GGWCC 2 cut(s) 1103, 1160
VspI ATTAAT 1 cut(s) 788
XagI CCTNNNNNAGG 1 cut(s) 348
XapI RAATTY 2 cut(s) 12, 1092
XceI RCATGY 3 cut(s) 1073, 1170, 1528
XmaI CCCGGG 1 cut(s) 139
XmiI GTMKAC 2 cut(s) 420, 744
XspI CTAG 1 cut(s) 809
ZraI GACGTC 1 cut(s) 589
Zsp2I ATGCAT 1 cut(s) 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.