FvH4_4g22140

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014075
Physical Location & Seq
Forward (+)
25080555 .. 25082421
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22140.t1

Sequence Viewer

Length: 909 bp
ATGGGTGTGAGGTTCTTCACTCTCCAAATGATCCCCATGTGTTTTCATGTCACTAATGTCTCCTCTCGCGTTCAGCTTCAGCCGGACGACGATGAGGAGGAGTACCAACCACCGCGGGCTAAGGTTGGTGCTAAGCGGGCGGTGGCTGGTAATATTAAGGTTGTTAAGTCCGACGGCCTTGTGAAGATTTACGACAAGCCGGTTGATGTATCAGAGCTTATGAGGCAGTTTCCTAAACACCTAGTGTGTGCTTCCGACTCGTTTTACATCGGCCAGAAAATCCCAGCTCTTTCGGAACACGATCAACTCCAGCTCGGCCAAAAGTACTTTCTGCTACCAAAGCACTTGTTCCAGTCCGTGCTCTCCTTCGTCACCATTGCCTCGTTCGCCTCTTCTGGACCGGAATCCGAACCCGAACAGAAGCCCTCGCCGGTTCCCTCCGCCAACTCCAAGAATGCCTTTCTAAGAAAGGCTGCGACTTGCGAGCCGTTTCATATACAGAAATCGGAAACCGGCTGCCTGAGAATACGTGTCTCCGACGAGTTTATATCGCAGTTGTTGGAGGAAGGCACGCTCGGGAACGAAGCTAAAGTCGAGGAAGAAGATGAACATAGTAAGAGTTTTGAGGCAAACAACAAAGTCTGCACCACGCCGCAGTTGAAGAAAGACTACTCACAACTGGTTGGGACAAGACAATGGAAGCCGAGGCTAGAGCCAATTAGAGAGACCCGGGAAACCAAGAGAAGATCAAGACTTTCTTCGTTTGGGATGAGAAGATCAAAGAAGAAATCTCAACCCAATTCATCATCAAAAGCAACAAAGAGTTCAAAGAGTCCAAAGACTGCTACTTCAGTAAAATCTGAGCACCATTCGAAAGCCAAGACTAAGATCAAATCATCGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

303

Amino Acids

33.98

Weight (kDa)

9.67

Isoelectric Point (pI)

56.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 49 - 242 8.7e-37 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 69, 115
AciI CCGC 6 cut(s) 113, 115, 136, 140, 441, 653
AclWI GGATC 1 cut(s) 25
AcoI YGGCCR 2 cut(s) 271, 316
AcuI CTGAAG 2 cut(s) 62, 834
AdeI CACNNNGTG 1 cut(s) 244
AfaI GTAC 2 cut(s) 104, 326
AflIII ACRYGT 1 cut(s) 529
AgsI TTSAA 2 cut(s) 661, 828
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AluBI AGCT 5 cut(s) 76, 217, 287, 313, 587
AluI AGCT 5 cut(s) 76, 217, 287, 313, 587
Alw21I GWGCWC 2 cut(s) 363, 867
Alw26I GTCTC 3 cut(s) 64, 538, 719
AlwI GGATC 1 cut(s) 25
Ama87I CYCGRG 2 cut(s) 575, 729
AoxI GGCC 3 cut(s) 175, 271, 316
ApeKI GCWGC 2 cut(s) 473, 516
ArsI GACNNNNNNTTYG 2 cut(s) 576, 608
AspS9I GGNCC 1 cut(s) 398
AsuC2I CCSGG 2 cut(s) 730, 731
AsuHPI GGTGA 1 cut(s) 364
AsuII TTCGAA 1 cut(s) 872
AvaI CYCGRG 2 cut(s) 575, 729
AvaII GGWCC 1 cut(s) 398
BarI GAAGNNNNNNTAC 2 cut(s) 653, 685
Bbv12I GWGCWC 2 cut(s) 363, 867
BbvI GCAGC 2 cut(s) 460, 503
BceAI ACGGC 2 cut(s) 190, 472
BcnI CCSGG 2 cut(s) 730, 731
BcoDI GTCTC 3 cut(s) 64, 538, 719
BfaI CTAG 2 cut(s) 242, 710
BisI GCNGC 3 cut(s) 474, 517, 653
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 3 cut(s) 475, 518, 654
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 2 cut(s) 730, 731
Bme18I GGWCC 1 cut(s) 398
BmeT110I CYCGRG 2 cut(s) 575, 729
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 2 cut(s) 730, 731
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 120
Bpu1102I GCTNAGC 1 cut(s) 132
Bpu14I TTCGAA 1 cut(s) 872
BpuMI CCSGG 2 cut(s) 730, 731
BsaAI YACGTR 1 cut(s) 530
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 3 cut(s) 113, 704, 729
BsaWI WCCGGW 1 cut(s) 400
Bse118I RCCGGY 3 cut(s) 199, 430, 512
Bse1I ACTGG 2 cut(s) 352, 684
Bse3DI GCAATG 1 cut(s) 375
BseDI CCNNGG 3 cut(s) 113, 704, 729
BseGI GGATG 1 cut(s) 774
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 512, 852
BseNI ACTGG 2 cut(s) 352, 684
BseRI GAGGAG 3 cut(s) 52, 110, 113
BseXI GCAGC 2 cut(s) 460, 503
BseYI CCCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 628
Bsh1236I CGCG 2 cut(s) 69, 115
BshFI GGCC 3 cut(s) 177, 273, 318
BsiHKAI GWGCWC 2 cut(s) 363, 867
BsiHKCI CYCGRG 2 cut(s) 575, 729
BsiSI CCGG 6 cut(s) 83, 200, 401, 431, 513, 730
BslFI GGGAC 1 cut(s) 700
BsmAI GTCTC 3 cut(s) 64, 538, 719
BsmFI GGGAC 1 cut(s) 700
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 3 cut(s) 177, 273, 318
Bso31I GGTCTC 1 cut(s) 719
BsoBI CYCGRG 2 cut(s) 575, 729
Bsp119I TTCGAA 1 cut(s) 872
Bsp1286I GDGCHC 2 cut(s) 363, 867
Bsp143I GATC 5 cut(s) 30, 301, 746, 776, 888
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 6 cut(s) 113, 115, 136, 140, 441, 653
BspANI GGCC 3 cut(s) 177, 273, 318
BspCNI CTCAG 2 cut(s) 513, 853
BspFNI CGCG 2 cut(s) 69, 115
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 1 cut(s) 25
BspT104I TTCGAA 1 cut(s) 872
BspTNI GGTCTC 1 cut(s) 719
BsrDI GCAATG 1 cut(s) 375
BsrFI RCCGGY 3 cut(s) 199, 430, 512
BsrI ACTGG 2 cut(s) 352, 684
BssAI RCCGGY 3 cut(s) 199, 430, 512
BssECI CCNNGG 3 cut(s) 113, 704, 729
BssMI GATC 5 cut(s) 30, 301, 746, 776, 888
Bst6I CTCTTC 1 cut(s) 397
BstBAI YACGTR 1 cut(s) 530
BstBI TTCGAA 1 cut(s) 872
BstC8I GCNNGC 4 cut(s) 117, 138, 485, 572
BstDEI CTNAG 6 cut(s) 120, 132, 464, 521, 861, 885
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 774
BstFNI CGCG 2 cut(s) 69, 115
BstKTI GATC 5 cut(s) 33, 304, 749, 779, 891
BstMAI GTCTC 3 cut(s) 64, 538, 719
BstMBI GATC 5 cut(s) 30, 301, 746, 776, 888
BstMWI GCNNNNNNNGC 4 cut(s) 137, 223, 340, 386
BstSCI CCNGG 2 cut(s) 728, 729
BstUI CGCG 2 cut(s) 69, 115
BstV1I GCAGC 2 cut(s) 460, 503
BsuRI GGCC 3 cut(s) 177, 273, 318
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 1 cut(s) 774
Cac8I GCNNGC 4 cut(s) 117, 138, 485, 572
Cfr10I RCCGGY 3 cut(s) 199, 430, 512
Cfr13I GGNCC 1 cut(s) 398
Cfr42I CCGCGG 1 cut(s) 116
Cfr9I CCCGGG 1 cut(s) 729
Csp6I GTAC 2 cut(s) 103, 325
CviAII CATG 2 cut(s) 37, 47
CviQI GTAC 2 cut(s) 103, 325
DdeI CTNAG 6 cut(s) 120, 132, 464, 521, 861, 885
DpnI GATC 5 cut(s) 32, 303, 748, 778, 890
DpnII GATC 5 cut(s) 30, 301, 746, 776, 888
DraIII CACNNNGTG 1 cut(s) 244
EaeI YGGCCR 2 cut(s) 271, 316
Eam1104I CTCTTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 397
EciI GGCGGA 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 719
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 2 cut(s) 62, 834
Eco88I CYCGRG 2 cut(s) 575, 729
FaeI CATG 2 cut(s) 40, 50
FaiI YATR 7 cut(s) 38, 48, 221, 495, 497, 548, 612
FalI AAGNNNNNCTT 4 cut(s) 443, 475, 742, 774
FaqI GGGAC 1 cut(s) 700
FatI CATG 2 cut(s) 36, 46
FauI CCCGC 2 cut(s) 108, 129
Fnu4HI GCNGC 3 cut(s) 474, 517, 653
FokI GGATG 1 cut(s) 781
Fsp4HI GCNGC 3 cut(s) 474, 517, 653
FspBI CTAG 2 cut(s) 242, 710
GluI GCNGC 3 cut(s) 474, 517, 653
GsaI CCCAGC 1 cut(s) 287
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 3 cut(s) 177, 273, 318
HapII CCGG 6 cut(s) 83, 200, 401, 431, 513, 730
Hin1II CATG 2 cut(s) 40, 50
HinfI GANTC 3 cut(s) 257, 404, 832
HpaII CCGG 6 cut(s) 83, 200, 401, 431, 513, 730
HphI GGTGA 1 cut(s) 364
Hpy188I TCNGA 8 cut(s) 172, 214, 256, 295, 409, 508, 538, 862
Hpy188III TCNNGA 3 cut(s) 396, 577, 750
Hpy99I CGWCG 3 cut(s) 92, 176, 542
HpyAV CCTTC 2 cut(s) 376, 560
HpyCH4IV ACGT 1 cut(s) 529
HpyCH4V TGCA 1 cut(s) 645
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 223, 340, 386
HpyF3I CTNAG 6 cut(s) 120, 132, 464, 521, 861, 885
HpySE526I ACGT 1 cut(s) 529
Hsp92II CATG 2 cut(s) 40, 50
KspI CCGCGG 1 cut(s) 116
Kzo9I GATC 5 cut(s) 30, 301, 746, 776, 888
Lsp1109I GCAGC 2 cut(s) 460, 503
MaeI CTAG 2 cut(s) 242, 710
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 2 cut(s) 49, 370
MalI GATC 5 cut(s) 32, 303, 748, 778, 890
MboI GATC 5 cut(s) 30, 301, 746, 776, 888
MhlI GDGCHC 2 cut(s) 363, 867
MluCI AATT 2 cut(s) 717, 799
MlyI GAGTC 2 cut(s) 251, 841
MmeI TCCRAC 4 cut(s) 195, 279, 540, 561
MseI TTAA 2 cut(s) 156, 165
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 6 cut(s) 83, 200, 401, 431, 513, 730
MspR9I CCNGG 2 cut(s) 730, 731
Mva1269I GAATGC 1 cut(s) 460
MvnI CGCG 2 cut(s) 69, 115
MwoI GCNNNNNNNGC 4 cut(s) 137, 223, 340, 386
NciI CCSGG 2 cut(s) 730, 731
NdeII GATC 5 cut(s) 30, 301, 746, 776, 888
NlaIII CATG 2 cut(s) 40, 50
NlaIV GGNNCC 1 cut(s) 435
NmeAIII GCCGAG 2 cut(s) 294, 729
NmuCI GTSAC 2 cut(s) 49, 370
NspV TTCGAA 1 cut(s) 872
PctI GAATGC 1 cut(s) 460
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 3 cut(s) 475, 518, 654
PleI GAGTC 2 cut(s) 251, 840
PpsI GAGTC 2 cut(s) 251, 840
Ppu21I YACGTR 1 cut(s) 530
PspFI CCCAGC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 1 cut(s) 398
RsaI GTAC 2 cut(s) 104, 326
RsaNI GTAC 2 cut(s) 103, 325
SacII CCGCGG 1 cut(s) 116
SaqAI TTAA 2 cut(s) 156, 165
SatI GCNGC 3 cut(s) 474, 517, 653
Sau3AI GATC 5 cut(s) 30, 301, 746, 776, 888
Sau96I GGNCC 1 cut(s) 398
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 2 cut(s) 251, 841
ScrFI CCNGG 2 cut(s) 730, 731
SduI GDGCHC 2 cut(s) 363, 867
Sfr303I CCGCGG 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 872
SgrBI CCGCGG 1 cut(s) 116
SinI GGWCC 1 cut(s) 398
SmaI CCCGGG 1 cut(s) 731
Sse9I AATT 2 cut(s) 717, 799
SsiI CCGC 6 cut(s) 113, 115, 136, 140, 441, 653
SspI AATATT 1 cut(s) 154
SspMI CTAG 2 cut(s) 242, 710
StyD4I CCNGG 2 cut(s) 728, 729
TaiI ACGT 1 cut(s) 532
TaqI TCGA 3 cut(s) 594, 872, 899
TasI AATT 2 cut(s) 717, 799
TatI WGTACW 1 cut(s) 324
TauI GCSGC 1 cut(s) 655
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 2 cut(s) 156, 165
Tru9I TTAA 2 cut(s) 156, 165
TseFI GTSAC 2 cut(s) 49, 370
TseI GCWGC 2 cut(s) 473, 516
Tsp45I GTSAC 2 cut(s) 49, 370
TspDTI ATGAA 4 cut(s) 35, 482, 621, 792
TspGWI ACGGA 1 cut(s) 346
TspMI CCCGGG 1 cut(s) 729
VpaK11BI GGWCC 1 cut(s) 398
XmaI CCCGGG 1 cut(s) 729
XspI CTAG 2 cut(s) 242, 710
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.