Rh4CG303500

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014075
Physical Location & Seq
Forward (+)
56373315 .. 56375886
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG303500.1

Sequence Viewer

Length: 948 bp
ATGGGTGTGAGGTTCTTCACTCTCCAAATGATCCCCATGTGTTTTCATGTCACGGGCAACCACCATGTCTCCTCTTGCGTCCAGCTACAACCGGACGACGATGAGGAGGAGTATCAACCACCGCGCGCCAAGGTAGGTGGTAAGCGTGCTAAGATAAGTGGTAAGCGCGCGGTGGTTGGTACCATCAAGGTCGTTAAGTCCGACGGCCTTGTGAAGATCTACAACAAGCCGATTGATGTATCAGAGCTTATGAGGCAATTTCCCAAGCACCTAGTGTGTGCTTCCGACTCATTTTACATAGGCCAGAAAATCCCAGCTCTTTCGGAACACGACCAGCTCCAGCTCGGCCGAAAGTACTTTCTGCTACCGAAGCACTTTTTCCAGACTGTGCTCTCCTTCGTCACCATTGCCTCGTTCGCCACCTCCGGACCCGAATCCGAACCCGAACAAAAGCCCTCGCCGGTTCCCTCCACCAATTCCAAGAATGCGTTTCTAAGAAAGGCCGCGAATTGCGAGCCTTTTCATATACAGAAATCGGAATCCGGTTGCCTGAGAATACGTGTCTCCGACGAGTTTATATCGCAATTGTTGGAGGAAGGCAAGCTCAGCGACGAAGCTAAAGTCGAAGAAGAAGATGAACATGGTAAGAGTTTTGAGCCAAACAACAGCAAAGTCTGTACCACGCCGCAGTTACAGAAAGACTACTCGCAACTGGTTGGATCAAGACAATGGAAGCCGAGGCTAGAGCCAATTAGAGAGACCCGGGAAACCAAGAGAAGATCAAGACTTTCTTCGTTTGGGATGAGAAGATCAAAGAAGAAATCTCAACCCAATTCATCAAAAGCAACACAGAGTTCAAAGGGTCCAAAGAGTCCAAAGATTGCTACTTTAGTAAAATCTGAGCACCATTCCAAAACCAAGATCAAGATCAAATCATCAAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

315

Amino Acids

35.54

Weight (kDa)

9.74

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 60 - 253 1.8e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 5 cut(s) 124, 126, 168, 170, 506
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 4 cut(s) 122, 170, 504, 686
AclWI GGATC 2 cut(s) 25, 727
AcoI YGGCCR 1 cut(s) 346
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 3 cut(s) 181, 356, 679
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 1 cut(s) 858
AluBI AGCT 7 cut(s) 85, 247, 317, 337, 343, 604, 617
AluI AGCT 7 cut(s) 85, 247, 317, 337, 343, 604, 617
Alw21I GWGCWC 2 cut(s) 393, 906
Alw26I GTCTC 3 cut(s) 73, 568, 752
AlwI GGATC 2 cut(s) 25, 727
Ama87I CYCGRG 1 cut(s) 762
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 4 cut(s) 205, 301, 346, 501
ArsI GACNNNNNNTTYG 2 cut(s) 606, 638
Asp718I GGTACC 1 cut(s) 179
AspLEI GCGC 4 cut(s) 126, 128, 168, 170
AspS9I GGNCC 2 cut(s) 428, 863
AsuC2I CCSGG 2 cut(s) 763, 764
AsuHPI GGTGA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 2 cut(s) 428, 863
BanI GGYRCC 1 cut(s) 179
Bbv12I GWGCWC 2 cut(s) 393, 906
BccI CCATC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 220
BcnI CCSGG 2 cut(s) 763, 764
BcoDI GTCTC 3 cut(s) 73, 568, 752
BfaI CTAG 2 cut(s) 272, 743
BglII AGATCT 1 cut(s) 216
BisI GCNGC 2 cut(s) 504, 686
BlpI GCTNAGC 1 cut(s) 605
BlsI GCNGC 2 cut(s) 505, 687
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 763, 764
Bme18I GGWCC 2 cut(s) 428, 863
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 2 cut(s) 428, 863
BmiI GGNNCC 4 cut(s) 181, 430, 465, 864
BmrFI CCNGG 2 cut(s) 763, 764
BpmI CTGGAG 1 cut(s) 323
Bpu1102I GCTNAGC 1 cut(s) 605
BpuMI CCSGG 2 cut(s) 763, 764
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 926
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 3 cut(s) 129, 737, 762
BsaWI WCCGGW 3 cut(s) 91, 425, 542
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 405
Bse8I GATNNNNATC 1 cut(s) 926
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 3 cut(s) 129, 737, 762
BseGI GGATG 1 cut(s) 807
BseJI GATNNNNATC 1 cut(s) 926
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 3 cut(s) 542, 619, 891
BseNI ACTGG 1 cut(s) 717
BsePI GCGCGC 2 cut(s) 124, 166
BseRI GAGGAG 3 cut(s) 61, 119, 122
BseX3I CGGCCG 1 cut(s) 346
BseYI CCCAGC 1 cut(s) 313
Bsh1236I CGCG 5 cut(s) 124, 126, 168, 170, 506
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 4 cut(s) 207, 303, 348, 503
BshNI GGYRCC 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 393, 906
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 5 cut(s) 92, 426, 461, 543, 763
BsmAI GTCTC 3 cut(s) 73, 568, 752
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 4 cut(s) 207, 303, 348, 503
Bso31I GGTCTC 1 cut(s) 752
BsoBI CYCGRG 1 cut(s) 762
Bsp1286I GDGCHC 2 cut(s) 393, 906
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
Bsp1720I GCTNAGC 1 cut(s) 605
BspACI CCGC 4 cut(s) 122, 170, 504, 686
BspANI GGCC 4 cut(s) 207, 303, 348, 503
BspCNI CTCAG 3 cut(s) 543, 618, 892
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 5 cut(s) 124, 126, 168, 170, 506
BspLI GGNNCC 4 cut(s) 181, 430, 465, 864
BspPI GGATC 2 cut(s) 25, 727
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 717
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 3 cut(s) 129, 737, 762
BssHII GCGCGC 2 cut(s) 124, 166
BssMI GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 5 cut(s) 126, 147, 168, 515, 602
BstDEI CTNAG 5 cut(s) 150, 494, 551, 605, 900
BstF5I GGATG 1 cut(s) 807
BstFNI CGCG 5 cut(s) 124, 126, 168, 170, 506
BstHHI GCGC 4 cut(s) 126, 128, 168, 170
BstKTI GATC 7 cut(s) 33, 219, 722, 782, 812, 924, 930
BstMAI GTCTC 3 cut(s) 73, 568, 752
BstMBI GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
BstSCI CCNGG 2 cut(s) 761, 762
BstUI CGCG 5 cut(s) 124, 126, 168, 170, 506
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BstZI CGGCCG 1 cut(s) 346
BsuRI GGCC 4 cut(s) 207, 303, 348, 503
BtsCI GGATG 1 cut(s) 807
Cac8I GCNNGC 5 cut(s) 126, 147, 168, 515, 602
CfoI GCGC 4 cut(s) 126, 128, 168, 170
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 428, 863
Cfr9I CCCGGG 1 cut(s) 762
CseI GACGC 1 cut(s) 67
Csp6I GTAC 3 cut(s) 180, 355, 678
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 4 cut(s) 37, 47, 65, 641
CviQI GTAC 3 cut(s) 180, 355, 678
DdeI CTNAG 5 cut(s) 150, 494, 551, 605, 900
DpnI GATC 7 cut(s) 32, 218, 721, 781, 811, 923, 929
DpnII GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 346
EagI CGGCCG 1 cut(s) 346
EclXI CGGCCG 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 752
Eco47I GGWCC 2 cut(s) 428, 863
Eco52I CGGCCG 1 cut(s) 346
Eco88I CYCGRG 1 cut(s) 762
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 4 cut(s) 40, 50, 68, 644
FaiI YATR 9 cut(s) 38, 48, 66, 251, 299, 525, 527, 578, 642
FalI AAGNNNNNCTT 2 cut(s) 775, 807
FatI CATG 4 cut(s) 36, 46, 64, 640
Fnu4HI GCNGC 2 cut(s) 504, 686
FokI GGATG 1 cut(s) 814
Fsp4HI GCNGC 2 cut(s) 504, 686
FspBI CTAG 2 cut(s) 272, 743
GlaI GCGC 4 cut(s) 125, 127, 167, 169
GluI GCNGC 2 cut(s) 504, 686
GsaI CCCAGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 323
HaeIII GGCC 4 cut(s) 207, 303, 348, 503
HapII CCGG 5 cut(s) 92, 426, 461, 543, 763
HgaI GACGC 1 cut(s) 67
HhaI GCGC 4 cut(s) 126, 128, 168, 170
Hin1II CATG 4 cut(s) 40, 50, 68, 644
Hin6I GCGC 4 cut(s) 124, 126, 166, 168
HinP1I GCGC 4 cut(s) 124, 126, 166, 168
HinfI GANTC 4 cut(s) 287, 434, 539, 871
HpaII CCGG 5 cut(s) 92, 426, 461, 543, 763
HphI GGTGA 1 cut(s) 394
Hpy188I TCNGA 8 cut(s) 202, 244, 286, 325, 439, 538, 568, 901
Hpy188III TCNNGA 5 cut(s) 382, 426, 723, 783, 925
Hpy99I CGWCG 4 cut(s) 101, 206, 572, 614
HpyAV CCTTC 2 cut(s) 406, 590
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 1 cut(s) 559
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
HpyF3I CTNAG 5 cut(s) 150, 494, 551, 605, 900
HpySE526I ACGT 1 cut(s) 559
Hsp92II CATG 4 cut(s) 40, 50, 68, 644
HspAI GCGC 4 cut(s) 124, 126, 166, 168
Kpn2I TCCGGA 1 cut(s) 425
KpnI GGTACC 1 cut(s) 183
Kzo9I GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
LmnI GCTCC 1 cut(s) 342
MaeI CTAG 2 cut(s) 272, 743
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 3 cut(s) 49, 400, 690
MalI GATC 7 cut(s) 32, 218, 721, 781, 811, 923, 929
MboI GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
MboII GAAGA 9 cut(s) 7, 226, 638, 641, 644, 783, 789, 819, 829
MfeI CAATTG 1 cut(s) 584
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 393, 906
MluCI AATT 6 cut(s) 257, 475, 508, 584, 750, 832
MlyI GAGTC 2 cut(s) 281, 880
MmeI TCCRAC 5 cut(s) 225, 309, 570, 591, 697
MroI TCCGGA 1 cut(s) 425
MseI TTAA 1 cut(s) 195
MspI CCGG 5 cut(s) 92, 426, 461, 543, 763
MspR9I CCNGG 2 cut(s) 763, 764
MunI CAATTG 1 cut(s) 584
Mva1269I GAATGC 1 cut(s) 490
MvnI CGCG 5 cut(s) 124, 126, 168, 170, 506
MwoI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
NciI CCSGG 2 cut(s) 763, 764
NdeII GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
NlaIII CATG 4 cut(s) 40, 50, 68, 644
NlaIV GGNNCC 4 cut(s) 181, 430, 465, 864
NmeAIII GCCGAG 2 cut(s) 324, 762
NmuCI GTSAC 2 cut(s) 49, 400
PauI GCGCGC 2 cut(s) 124, 166
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 434, 539
PkrI GCNGC 2 cut(s) 505, 687
PleI GAGTC 2 cut(s) 281, 879
PpsI GAGTC 2 cut(s) 281, 879
Ppu21I YACGTR 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 313
PspN4I GGNNCC 4 cut(s) 181, 430, 465, 864
PspPI GGNCC 2 cut(s) 428, 863
PsuI RGATCY 1 cut(s) 216
PteI GCGCGC 2 cut(s) 124, 166
RsaI GTAC 3 cut(s) 181, 356, 679
RsaNI GTAC 3 cut(s) 180, 355, 678
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 2 cut(s) 504, 686
Sau3AI GATC 7 cut(s) 30, 216, 719, 779, 809, 921, 927
Sau96I GGNCC 2 cut(s) 428, 863
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 2 cut(s) 281, 880
ScrFI CCNGG 2 cut(s) 763, 764
SduI GDGCHC 2 cut(s) 393, 906
SinI GGWCC 2 cut(s) 428, 863
SmaI CCCGGG 1 cut(s) 764
Sse9I AATT 6 cut(s) 257, 475, 508, 584, 750, 832
SsiI CCGC 4 cut(s) 122, 170, 504, 686
SspMI CTAG 2 cut(s) 272, 743
StyD4I CCNGG 2 cut(s) 761, 762
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 1 cut(s) 562
TaqI TCGA 1 cut(s) 624
TasI AATT 6 cut(s) 257, 475, 508, 584, 750, 832
TatI WGTACW 1 cut(s) 354
TauI GCSGC 2 cut(s) 506, 688
TfiI GAWTC 2 cut(s) 434, 539
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseFI GTSAC 2 cut(s) 49, 400
Tsp45I GTSAC 2 cut(s) 49, 400
TspDTI ATGAA 4 cut(s) 35, 512, 651, 825
TspMI CCCGGG 1 cut(s) 762
VpaK11BI GGWCC 2 cut(s) 428, 863
XmaI CCCGGG 1 cut(s) 762
XspI CTAG 2 cut(s) 272, 743
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.