pycom16g11450

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014075
Physical Location & Seq
Reverse (-)
7966826 .. 7967602
777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g11450.1

Sequence Viewer

Length: 777 bp
ATGACGTTGCGATTCTTCACTCTTCAAATGATGCCGTGGTGCTTCCAGTTAATGGGCAGCCACCGCATCTCTCGCGCGCAGCTCCGTCCCAACGACCAGGCCCGCCCGATCTGCACGATCAAGCTTGTCAAATCGGACGGGCTTGTGTTGATCTACCACCGGCCAATCCACGTTTCGGAAGTCATGACGGAGTTTCCGAAACACCAAGTGTGCCGGTCCGATTCGTTCTACATCGGGCAGAAGATCCCGGCACTGTCGGAGGACGACCAGCTCCAGCTCGGTCACAAGTACTTTCTCCTCCCCCAGCAGTTTTTCCAATCCGTGCTTTCGTTCGTCACCATTGCTTCCTTCAGCAGAAAAGACTCGTCGAGAAACGCGTTTGTTAGAAAAGCGGCGAGCTGCGAGCCGTTCGATATACAGAAAACGCCGTCTGGGTGTTTGAGAATACGCGTTTCGGACGAGTTTATATCGCAATTGCTGGAGGAAAAGAAGCTCGAGGAGGACGCGGCGGCGGTGGAGGCCAAGAGCGGGGTTTGTACGACGGCTCAGCTGCAGAAAGACTACACACAGCTTGTTGGGGCACGGCAGTGGAAGCCGAGGCTCGAGCCCATCAGAGAGGCTCGGGAGAAGAGCAGAAAGCTTTCTTCTTTTGGGCTGAGGAGATCTTCCAAGAAGAAATCTCAACCAAATTCATCGAAGGCTTCCCAGAAGCAAAAGAGTAGTTCTACTAATGGTTCTTCCAAAGCCAAGATCCAGATCAAATCGTCGAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

259

Amino Acids

29.42

Weight (kDa)

10.05

Isoelectric Point (pI)

58.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 38 - 206 1.6e-35 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 52
AccBSI CCGCTC 1 cut(s) 528
AccII CGCG 5 cut(s) 75, 77, 377, 450, 506
AciI CCGC 7 cut(s) 64, 103, 392, 506, 509, 512, 528
AclWI GGATC 2 cut(s) 238, 745
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 1 cut(s) 334
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 2 cut(s) 290, 538
AfiI CCNNNNNNNGG 3 cut(s) 52, 175, 528
AflIII ACRYGT 2 cut(s) 375, 448
AgsI TTSAA 1 cut(s) 26
AjnI CCWGG 1 cut(s) 96
AjuI GAANNNNNNNTTGG 2 cut(s) 157, 189
AleI CACNNNNGTG 1 cut(s) 586
AluBI AGCT 9 cut(s) 82, 124, 271, 277, 399, 493, 550, 571, 640
AluI AGCT 9 cut(s) 82, 124, 271, 277, 399, 493, 550, 571, 640
AlwI GGATC 2 cut(s) 238, 745
Ama87I CYCGRG 3 cut(s) 494, 602, 621
AoxI GGCC 3 cut(s) 99, 161, 519
ApeKI GCWGC 4 cut(s) 57, 79, 399, 550
ApoI RAATTY 1 cut(s) 688
Asp700I GAANNNNTTC 1 cut(s) 640
AspLEI GCGC 2 cut(s) 77, 79
AspS9I GGNCC 2 cut(s) 100, 216
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 328
AvaI CYCGRG 3 cut(s) 494, 602, 621
AvaII GGWCC 1 cut(s) 216
BaeGI GKGCMC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 609
BbvCI CCTCAGC 1 cut(s) 656
BbvI GCAGC 4 cut(s) 69, 91, 386, 537
BccI CCATC 1 cut(s) 617
BceAI ACGGC 5 cut(s) 19, 391, 412, 558, 599
BciT130I CCWGG 1 cut(s) 98
BcnI CCSGG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 551
BglII AGATCT 1 cut(s) 662
BisI GCNGC 7 cut(s) 58, 80, 393, 400, 507, 510, 551
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 7 cut(s) 59, 81, 394, 401, 508, 511, 552
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 2 cut(s) 98, 248
Bme18I GGWCC 1 cut(s) 216
BmeT110I CYCGRG 3 cut(s) 494, 602, 621
BmgT120I GGNCC 2 cut(s) 100, 216
BmrFI CCNGG 2 cut(s) 98, 248
BmsI GCATC 2 cut(s) 21, 75
BpmI CTGGAG 2 cut(s) 257, 500
Bpu10I CCTNAGC 1 cut(s) 656
Bpu1102I GCTNAGC 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 755
BsaJI CCNNGG 2 cut(s) 35, 596
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 175, 528
Bse118I RCCGGY 2 cut(s) 159, 213
Bse1I ACTGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 339
Bse8I GATNNNNATC 1 cut(s) 755
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 35, 596
BseJI GATNNNNATC 1 cut(s) 755
BseLI CCNNNNNNNGG 3 cut(s) 52, 175, 528
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 2 cut(s) 560, 647
BseNI ACTGG 1 cut(s) 46
BsePI GCGCGC 1 cut(s) 75
BseRI GAGGAG 3 cut(s) 287, 512, 673
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 4 cut(s) 69, 91, 386, 537
BseYI CCCAGC 1 cut(s) 303
BsgI GTGCAG 1 cut(s) 97
Bsh1236I CGCG 5 cut(s) 75, 77, 377, 450, 506
BshFI GGCC 3 cut(s) 101, 163, 521
BsiHKCI CYCGRG 3 cut(s) 494, 602, 621
BsiSI CCGG 3 cut(s) 160, 214, 248
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 3 cut(s) 52, 175, 528
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 3 cut(s) 101, 163, 521
BsoBI CYCGRG 3 cut(s) 494, 602, 621
Bsp1286I GDGCHC 2 cut(s) 583, 609
Bsp143I GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 7 cut(s) 64, 103, 392, 506, 509, 512, 528
BspANI GGCC 3 cut(s) 101, 163, 521
BspCNI CTCAG 2 cut(s) 559, 648
BspFNI CGCG 5 cut(s) 75, 77, 377, 450, 506
BspHI TCATGA 1 cut(s) 183
BspMAI CTGCAG 1 cut(s) 555
BspPI GGATC 2 cut(s) 238, 745
BspQI GCTCTTC 1 cut(s) 623
BsrBI CCGCTC 1 cut(s) 528
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 2 cut(s) 159, 213
BsrI ACTGG 1 cut(s) 46
BssAI RCCGGY 2 cut(s) 159, 213
BssECI CCNNGG 2 cut(s) 35, 596
BssHII GCGCGC 1 cut(s) 75
BssMI GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 2 cut(s) 27, 623
BstC8I GCNNGC 4 cut(s) 77, 103, 397, 404
BstDEI CTNAG 2 cut(s) 546, 656
BstDSI CCRYGG 1 cut(s) 35
BstFNI CGCG 5 cut(s) 75, 77, 377, 450, 506
BstHHI GCGC 2 cut(s) 77, 79
BstKTI GATC 7 cut(s) 111, 120, 153, 246, 665, 753, 759
BstMBI GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
BstMWI GCNNNNNNNGC 5 cut(s) 63, 72, 111, 518, 592
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 96, 246
BstSFI CTRYAG 1 cut(s) 551
BstSLI GKGCMC 1 cut(s) 583
BstUI CGCG 5 cut(s) 75, 77, 377, 450, 506
BstV1I GCAGC 4 cut(s) 69, 91, 386, 537
BstX2I RGATCY 3 cut(s) 243, 662, 750
BstYI RGATCY 3 cut(s) 243, 662, 750
BsuRI GGCC 3 cut(s) 101, 163, 521
BtgI CCRYGG 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 593
BtsIMutI CAGTG 2 cut(s) 251, 593
Cac8I GCNNGC 4 cut(s) 77, 103, 397, 404
CciI TCATGA 1 cut(s) 183
CfoI GCGC 2 cut(s) 77, 79
Cfr10I RCCGGY 2 cut(s) 159, 213
Cfr13I GGNCC 2 cut(s) 100, 216
CpoI CGGWCCG 1 cut(s) 216
CseI GACGC 1 cut(s) 512
Csp6I GTAC 2 cut(s) 289, 537
CspI CGGWCCG 1 cut(s) 216
CviAII CATG 1 cut(s) 184
CviQI GTAC 2 cut(s) 289, 537
DdeI CTNAG 2 cut(s) 546, 656
DpnI GATC 7 cut(s) 110, 119, 152, 245, 664, 752, 758
DpnII GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
DraIII CACNNNGTG 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 2 cut(s) 27, 623
EarI CTCTTC 2 cut(s) 27, 623
Eco24I GRGCYC 1 cut(s) 609
Eco47I GGWCC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 334
Eco88I CYCGRG 3 cut(s) 494, 602, 621
EcoRII CCWGG 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 609
FaeI CATG 1 cut(s) 187
FaiI YATR 3 cut(s) 185, 416, 467
FaqI GGGAC 1 cut(s) 72
FatI CATG 1 cut(s) 183
FauI CCCGC 2 cut(s) 110, 521
Fnu4HI GCNGC 7 cut(s) 58, 80, 393, 400, 507, 510, 551
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 7 cut(s) 58, 80, 393, 400, 507, 510, 551
GlaI GCGC 2 cut(s) 76, 78
GluI GCNGC 7 cut(s) 58, 80, 393, 400, 507, 510, 551
GsaI CCCAGC 1 cut(s) 307
GsuI CTGGAG 2 cut(s) 257, 500
HaeIII GGCC 3 cut(s) 101, 163, 521
HapII CCGG 3 cut(s) 160, 214, 248
HgaI GACGC 1 cut(s) 512
HhaI GCGC 2 cut(s) 77, 79
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 2 cut(s) 75, 77
HinP1I GCGC 2 cut(s) 75, 77
HindIII AAGCTT 2 cut(s) 122, 638
HinfI GANTC 3 cut(s) 12, 221, 362
HpaII CCGG 3 cut(s) 160, 214, 248
HphI GGTGA 1 cut(s) 328
Hpy188I TCNGA 7 cut(s) 136, 178, 198, 220, 259, 457, 614
Hpy188III TCNNGA 5 cut(s) 184, 369, 623, 754, 768
Hpy99I CGWCG 3 cut(s) 370, 544, 769
HpyAV CCTTC 2 cut(s) 358, 691
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 2 cut(s) 5, 171
HpyCH4V TGCA 2 cut(s) 114, 553
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 72, 111, 518, 592
HpyF3I CTNAG 2 cut(s) 546, 656
HpySE526I ACGT 2 cut(s) 5, 171
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 2 cut(s) 75, 77
Kzo9I GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
LguI GCTCTTC 1 cut(s) 623
LmnI GCTCC 2 cut(s) 87, 276
Lsp1109I GCAGC 4 cut(s) 69, 91, 386, 537
LweI GCATC 2 cut(s) 21, 75
MaeII ACGT 2 cut(s) 5, 171
MaeIII GTNAC 2 cut(s) 281, 334
MalI GATC 7 cut(s) 110, 119, 152, 245, 664, 752, 758
MbiI CCGCTC 1 cut(s) 528
MboI GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
MboII GAAGA 8 cut(s) 7, 14, 253, 636, 640, 657, 685, 729
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 3 cut(s) 243, 662, 750
MhlI GDGCHC 2 cut(s) 583, 609
MluCI AATT 2 cut(s) 473, 688
MluI ACGCGT 2 cut(s) 375, 448
MlyI GAGTC 1 cut(s) 356
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 9 cut(s) 253, 308, 475, 490, 493, 511, 591, 610, 651
MroXI GAANNNNTTC 1 cut(s) 640
MseI TTAA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 586
MspA1I CMGCKG 1 cut(s) 550
MspI CCGG 3 cut(s) 160, 214, 248
MspR9I CCNGG 2 cut(s) 98, 248
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 5 cut(s) 75, 77, 377, 450, 506
MwoI GCNNNNNNNGC 5 cut(s) 63, 72, 111, 518, 592
NciI CCSGG 1 cut(s) 248
NdeII GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
NlaIII CATG 1 cut(s) 187
NmeAIII GCCGAG 1 cut(s) 621
NmuCI GTSAC 2 cut(s) 281, 334
OliI CACNNNNGTG 1 cut(s) 586
PaeR7I CTCGAG 2 cut(s) 494, 602
PagI TCATGA 1 cut(s) 183
PauI GCGCGC 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 623
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 374
PdmI GAANNNNTTC 1 cut(s) 640
PfeI GAWTC 2 cut(s) 12, 221
PflMI CCANNNNNTGG 1 cut(s) 52
PkrI GCNGC 7 cut(s) 59, 81, 394, 401, 508, 511, 552
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 96
PspFI CCCAGC 1 cut(s) 303
PspGI CCWGG 1 cut(s) 96
PspPI GGNCC 2 cut(s) 100, 216
PspXI VCTCGAGB 2 cut(s) 494, 602
PstI CTGCAG 1 cut(s) 555
PsuI RGATCY 3 cut(s) 243, 662, 750
PteI GCGCGC 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 550
RsaI GTAC 2 cut(s) 290, 538
RsaNI GTAC 2 cut(s) 289, 537
RseI CAYNNNNRTG 1 cut(s) 586
Rsr2I CGGWCCG 1 cut(s) 216
RsrII CGGWCCG 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 623
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 7 cut(s) 58, 80, 393, 400, 507, 510, 551
Sau3AI GATC 7 cut(s) 108, 117, 150, 243, 662, 750, 756
Sau96I GGNCC 2 cut(s) 100, 216
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 1 cut(s) 356
ScrFI CCNGG 2 cut(s) 98, 248
SduI GDGCHC 2 cut(s) 583, 609
SfaNI GCATC 2 cut(s) 21, 75
SfcI CTRYAG 1 cut(s) 551
Sfr274I CTCGAG 2 cut(s) 494, 602
SinI GGWCC 1 cut(s) 216
SlaI CTCGAG 2 cut(s) 494, 602
SmiMI CAYNNNNRTG 1 cut(s) 586
SmlI CTYRAG 2 cut(s) 494, 602
SmoI CTYRAG 2 cut(s) 494, 602
Sse9I AATT 2 cut(s) 473, 688
SsiI CCGC 7 cut(s) 64, 103, 392, 506, 509, 512, 528
StyD4I CCNGG 2 cut(s) 96, 246
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 2 cut(s) 8, 174
TaqI TCGA 6 cut(s) 368, 411, 495, 603, 695, 767
TaqII GACCGA 1 cut(s) 269
TasI AATT 2 cut(s) 473, 688
TatI WGTACW 1 cut(s) 288
TauI GCSGC 3 cut(s) 395, 509, 512
TfiI GAWTC 2 cut(s) 12, 221
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 2 cut(s) 258, 593
TseFI GTSAC 2 cut(s) 281, 334
TseI GCWGC 4 cut(s) 57, 79, 399, 550
Tsp45I GTSAC 2 cut(s) 281, 334
TspDTI ATGAA 1 cut(s) 681
TspGWI ACGGA 3 cut(s) 74, 203, 310
TspRI CASTG 2 cut(s) 258, 593
Van91I CCANNNNNTGG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 216
XapI RAATTY 1 cut(s) 688
XhoI CTCGAG 2 cut(s) 494, 602
XmnI GAANNNNTTC 1 cut(s) 640
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.