pycom13g11710

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014075
Physical Location & Seq
Reverse (-)
8078144 .. 8078929
786 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g11710.1

Sequence Viewer

Length: 786 bp
ATGAAGTTGCGGTTCTTCGCACACCAAATGGTGCCGTGGTGCTTCCAATTAATGGGCAGACACCACATATCTCGCGCGCGGCTCCGTTCCAAAGACCAAGCCCGCCCGATCGGCACCATCAAGCTCGTCAAATCGGACGGGCTTGTGTTGGTCTACCACCAACCAATCCACGTTTCGGAAGTCATGACGGAGTTTCCGAAACACCAAGTGTGCCGGTCCGATACGTTGTACATCGGGCAGAAGATCCCGGCACTGTCGGAGGACGACCAGCTCCAGCTCGGCCACAAGTACTTTCTCCTCCCCCAGCAGTTTTTCCAATCCGTGCTTTCGTTCGTCACCATTGCTTCCTTCAGCAACACCAAAGACTCGTCGAGAAACGCATTTCTTAGAAAGGCCGCGAGCTGCGAGCCGTTCGATATACAGAAAACGCCTTCTGGATGTTTGAGAATACGCGTTTCGGATGAGTTTATATCGCAGCTGCTGGAGGAAAAGAAGTTCAATGAGGAGGAGGACACGGCGACGACGAAGGCCAAGAGCAGGGTCTGCACGACGGCTCAGCTGCAGAAAGACTACACGCAACTTGTTGGGTCACGGCAGTGGAAGCCAAGGCTCGAACCCATCAGAGAGGTTCGGGAGAAGAGAAGAAAGCTTTCTTCTTTTGGGATGAGAAGATCTTGCAAGAAGAAATCTCAGGTGAATTCATCCAAGGCTTCTCAGAAGCAAAAGAGTAGTTCTGCTAATGGTTCTTCCAAAGCCAAGATCAAGATTAAATCATCAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

262

Amino Acids

30.01

Weight (kDa)

10.19

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 37 - 209 6.4e-35 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 31, 113
AccB7I CCANNNNNTGG 1 cut(s) 52
AccI GTMKAC 1 cut(s) 153
AccII CGCG 5 cut(s) 75, 77, 79, 398, 453
AciI CCGC 4 cut(s) 10, 79, 103, 396
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 334
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 2 cut(s) 230, 290
AfiI CCNNNNNNNGG 3 cut(s) 52, 175, 537
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 157, 189
AleI CACNNNNGTG 1 cut(s) 595
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
AluBI AGCT 7 cut(s) 124, 271, 277, 402, 478, 559, 649
AluI AGCT 7 cut(s) 124, 271, 277, 402, 478, 559, 649
AlwI GGATC 1 cut(s) 238
AlwNI CAGNNNCTG 2 cut(s) 481, 543
AoxI GGCC 3 cut(s) 280, 393, 528
ApeKI GCWGC 4 cut(s) 402, 475, 478, 559
ApoI RAATTY 1 cut(s) 697
AseI ATTAAT 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 649
AspLEI GCGC 2 cut(s) 77, 79
AspS9I GGNCC 1 cut(s) 216
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 328, 706
AvaII GGWCC 1 cut(s) 216
BanI GGYRCC 2 cut(s) 31, 113
BbvI GCAGC 4 cut(s) 389, 465, 487, 546
BccI CCATC 2 cut(s) 125, 626
BceAI ACGGC 5 cut(s) 19, 394, 531, 567, 608
BcnI CCSGG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 560
BglI GCCNNNNNGGC 1 cut(s) 111
BglII AGATCT 1 cut(s) 671
BisI GCNGC 6 cut(s) 80, 396, 403, 476, 479, 560
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 6 cut(s) 81, 397, 404, 477, 480, 561
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 216
BmiI GGNNCC 3 cut(s) 33, 83, 115
BmrFI CCNGG 1 cut(s) 248
BpmI CTGGAG 2 cut(s) 257, 503
Bpu1102I GCTNAGC 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 248
BsaJI CCNNGG 3 cut(s) 35, 605, 705
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 175, 537
Bse118I RCCGGY 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 339
BseDI CCNNGG 3 cut(s) 35, 605, 705
BseGI GGATG 4 cut(s) 443, 466, 669, 701
BseLI CCNNNNNNNGG 3 cut(s) 52, 175, 537
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 3 cut(s) 569, 704, 728
BsePI GCGCGC 1 cut(s) 75
BseRI GAGGAG 3 cut(s) 287, 518, 521
BseXI GCAGC 4 cut(s) 389, 465, 487, 546
BseYI CCCAGC 1 cut(s) 303
BsgI GTGCAG 1 cut(s) 529
Bsh1236I CGCG 5 cut(s) 75, 77, 79, 398, 453
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 282, 395, 530
BshNI GGYRCC 2 cut(s) 31, 113
BsiEI CGRYCG 1 cut(s) 111
BsiSI CCGG 2 cut(s) 214, 248
BslI CCNNNNNNNGG 3 cut(s) 52, 175, 537
BsnI GGCC 3 cut(s) 282, 395, 530
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 4 cut(s) 108, 243, 671, 759
Bsp1720I GCTNAGC 1 cut(s) 555
BspACI CCGC 4 cut(s) 10, 79, 103, 396
BspANI GGCC 3 cut(s) 282, 395, 530
BspCNI CTCAG 3 cut(s) 568, 703, 727
BspFNI CGCG 5 cut(s) 75, 77, 79, 398, 453
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 3 cut(s) 33, 83, 115
BspMAI CTGCAG 1 cut(s) 564
BspPI GGATC 1 cut(s) 238
BspT107I GGYRCC 2 cut(s) 31, 113
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 3 cut(s) 35, 605, 705
BssHII GCGCGC 1 cut(s) 75
BssMI GATC 4 cut(s) 108, 243, 671, 759
BssT1I CCWWGG 2 cut(s) 605, 705
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 632
BstAPI GCANNNNNTGC 1 cut(s) 543
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 4 cut(s) 77, 103, 400, 407
BstDEI CTNAG 4 cut(s) 386, 555, 690, 714
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 4 cut(s) 443, 466, 669, 701
BstFNI CGCG 5 cut(s) 75, 77, 79, 398, 453
BstHHI GCGC 2 cut(s) 77, 79
BstKTI GATC 4 cut(s) 111, 246, 674, 762
BstMBI GATC 4 cut(s) 108, 243, 671, 759
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 111, 543, 601
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 560
BstUI CGCG 5 cut(s) 75, 77, 79, 398, 453
BstV1I GCAGC 4 cut(s) 389, 465, 487, 546
BstX2I RGATCY 2 cut(s) 243, 671
BstYI RGATCY 2 cut(s) 243, 671
BsuRI GGCC 3 cut(s) 282, 395, 530
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 4 cut(s) 443, 466, 669, 701
BtsI GCAGTG 1 cut(s) 602
BtsIMutI CAGTG 2 cut(s) 251, 602
Cac8I GCNNGC 4 cut(s) 77, 103, 400, 407
CaiI CAGNNNCTG 2 cut(s) 481, 543
CciI TCATGA 1 cut(s) 183
CfoI GCGC 2 cut(s) 77, 79
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 216
CpoI CGGWCCG 1 cut(s) 216
Csp6I GTAC 2 cut(s) 229, 289
CspI CGGWCCG 1 cut(s) 216
CviAII CATG 1 cut(s) 184
CviQI GTAC 2 cut(s) 229, 289
DdeI CTNAG 4 cut(s) 386, 555, 690, 714
DpnI GATC 4 cut(s) 110, 245, 673, 761
DpnII GATC 4 cut(s) 108, 243, 671, 759
DraIII CACNNNGTG 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
Eco130I CCWWGG 2 cut(s) 605, 705
Eco47I GGWCC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 334
EcoRI GAATTC 1 cut(s) 697
EcoT14I CCWWGG 2 cut(s) 605, 705
ErhI CCWWGG 2 cut(s) 605, 705
FaeI CATG 1 cut(s) 187
FaiI YATR 4 cut(s) 68, 185, 419, 470
FatI CATG 1 cut(s) 183
FauI CCCGC 1 cut(s) 110
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 6 cut(s) 80, 396, 403, 476, 479, 560
FokI GGATG 4 cut(s) 450, 473, 676, 688
Fsp4HI GCNGC 6 cut(s) 80, 396, 403, 476, 479, 560
GlaI GCGC 2 cut(s) 76, 78
GluI GCNGC 6 cut(s) 80, 396, 403, 476, 479, 560
GsaI CCCAGC 1 cut(s) 307
GsuI CTGGAG 2 cut(s) 257, 503
HaeIII GGCC 3 cut(s) 282, 395, 530
HapII CCGG 2 cut(s) 214, 248
HhaI GCGC 2 cut(s) 77, 79
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 2 cut(s) 75, 77
HinP1I GCGC 2 cut(s) 75, 77
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 1 cut(s) 365
HpaII CCGG 2 cut(s) 214, 248
HphI GGTGA 2 cut(s) 328, 706
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 8 cut(s) 136, 178, 198, 220, 259, 460, 623, 717
Hpy188III TCNNGA 6 cut(s) 184, 372, 435, 632, 763, 777
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 4 cut(s) 373, 523, 526, 553
HpyAV CCTTC 3 cut(s) 358, 441, 520
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 2 cut(s) 171, 224
HpyCH4V TGCA 3 cut(s) 546, 562, 678
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 543, 601
HpyF3I CTNAG 4 cut(s) 386, 555, 690, 714
HpySE526I ACGT 2 cut(s) 171, 224
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 2 cut(s) 75, 77
Kzo9I GATC 4 cut(s) 108, 243, 671, 759
LmnI GCTCC 2 cut(s) 87, 276
LpnPI CCDG 9 cut(s) 227, 261, 281, 287, 317, 420, 467, 523, 677
Lsp1109I GCAGC 4 cut(s) 389, 465, 487, 546
MaeII ACGT 2 cut(s) 171, 224
MaeIII GTNAC 2 cut(s) 334, 588
MalI GATC 4 cut(s) 110, 245, 673, 761
MauBI CGCGCGCG 1 cut(s) 75
MboI GATC 4 cut(s) 108, 243, 671, 759
MboII GAAGA 8 cut(s) 7, 253, 645, 649, 654, 681, 694, 738
MflI RGATCY 2 cut(s) 243, 671
MluCI AATT 2 cut(s) 47, 697
MluI ACGCGT 1 cut(s) 451
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 7 cut(s) 253, 308, 478, 496, 499, 502, 619
MroXI GAANNNNTTC 1 cut(s) 649
MseI TTAA 2 cut(s) 50, 768
MslI CAYNNNNRTG 1 cut(s) 595
MspA1I CMGCKG 2 cut(s) 478, 559
MspI CCGG 2 cut(s) 214, 248
MspR9I CCNGG 1 cut(s) 248
MvnI CGCG 5 cut(s) 75, 77, 79, 398, 453
MwoI GCNNNNNNNGC 3 cut(s) 111, 543, 601
NciI CCSGG 1 cut(s) 248
NdeII GATC 4 cut(s) 108, 243, 671, 759
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 3 cut(s) 33, 83, 115
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 2 cut(s) 334, 588
OliI CACNNNNGTG 1 cut(s) 595
PagI TCATGA 1 cut(s) 183
PauI GCGCGC 1 cut(s) 75
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 521
PdmI GAANNNNTTC 1 cut(s) 649
PflMI CCANNNNNTGG 1 cut(s) 52
PkrI GCNGC 6 cut(s) 81, 397, 404, 477, 480, 561
Ple19I CGATCG 1 cut(s) 111
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
PshBI ATTAAT 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 303
PspN4I GGNNCC 3 cut(s) 33, 83, 115
PspPI GGNCC 1 cut(s) 216
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 2 cut(s) 481, 543
PsuI RGATCY 2 cut(s) 243, 671
PteI GCGCGC 1 cut(s) 75
PvuI CGATCG 1 cut(s) 111
PvuII CAGCTG 2 cut(s) 478, 559
RsaI GTAC 2 cut(s) 230, 290
RsaNI GTAC 2 cut(s) 229, 289
RseI CAYNNNNRTG 1 cut(s) 595
Rsr2I CGGWCCG 1 cut(s) 216
RsrII CGGWCCG 1 cut(s) 216
SaqAI TTAA 2 cut(s) 50, 768
SatI GCNGC 6 cut(s) 80, 396, 403, 476, 479, 560
Sau3AI GATC 4 cut(s) 108, 243, 671, 759
Sau96I GGNCC 1 cut(s) 216
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 248
SfcI CTRYAG 1 cut(s) 560
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 595
Sse9I AATT 2 cut(s) 47, 697
SsiI CCGC 4 cut(s) 10, 79, 103, 396
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 2 cut(s) 605, 705
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 2 cut(s) 174, 227
TaqI TCGA 3 cut(s) 371, 414, 612
TasI AATT 2 cut(s) 47, 697
TatI WGTACW 2 cut(s) 228, 288
TauI GCSGC 2 cut(s) 82, 398
Tru1I TTAA 2 cut(s) 50, 768
Tru9I TTAA 2 cut(s) 50, 768
TscAI CASTG 2 cut(s) 258, 602
TseFI GTSAC 2 cut(s) 334, 588
TseI GCWGC 4 cut(s) 402, 475, 478, 559
Tsp45I GTSAC 2 cut(s) 334, 588
TspDTI ATGAA 2 cut(s) 17, 690
TspGWI ACGGA 3 cut(s) 74, 203, 310
TspRI CASTG 2 cut(s) 258, 602
Van91I CCANNNNNTGG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 216
VspI ATTAAT 1 cut(s) 50
XapI RAATTY 1 cut(s) 697
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 649
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.