RLG00000007156

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014075
Physical Location & Seq
Reverse (-)
14099695 .. 14101346
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007156

Sequence Viewer

Length: 933 bp
ATGGGTGTGAGGTTCTTCACTCTCCAAATGATCCCCATGTGTTTTCATGTCACGGGCAACCACCATGTCTCCTCTTGCGTCCAGCTCCAACCGGACGACGACGATGAGGAGTATCAACCACCGCGCGCCAGGGTAGGTGGTAAGCGTGCTAAGGTAGGTGGTAAGCGCGCTGTGGTTGGTACTATCAAGGTCGTTAAGTCCGACGGCCTTGTGAAGATCTACAACAAGCCGATTGATGTATCAGAGCTTATGAGGCAATTTCCCAAGCACCTAGTGTGTGCTTCCGACTCATTTTACATCGGCCAGAAAATCCCAGCTCTTTCGGAACACGACCAGCTCCAGCTCGGCCAAAAGTACTTTCTGCTACCGAAGCACTTTTTCCAGACTGTGCTATCCTTCGTCACCATTGCCTCGTTCGCCACCTCCGGACCCGAATCTGAACCCGAACAAAAGCCCTCGCCGGTTCCCTCCACCAATTCCAAGAATGCGTTTCTAAGAAAGGCCGCGAATTGCGAGCCTTTTCATATACAGAAATCGGAATCCGGTTGCCTGAGAATACGTGTCTCCGACGAGTTTATATCGCAATTGTTGGAGGAAGGCAAGCTCAGCGACGAAGCTAAAGTCGAAGAAGAAGATGAACACGGTAAGAGTTTTGAGCCAAACAACAGCAAAGTCTGTACCACGCCGCAGTTACAGAAAGACTACTCGCAACTGGTTGGATCAAGACAGTGGAAGCCGAGGCTCGAGCCAATTAGAGAGACCCGGGAAACCAAGAGAAGATCAAGACTTTCTTCGTTTGGGATGAGAAGATCAAAGAAGAAATCTCAGCCCAATTCATCAAAAGCAACACAGAGTTCAAAGAGTCCAAAGAGTCCAAAGTCTGGAGCCCCGTGGGATTTCCGCGGCGGCTATAATTATAGAAATGGATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

311

Amino Acids

35.02

Weight (kDa)

9.54

Isoelectric Point (pI)

54.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 60 - 253 1.1e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 881
AccII CGCG 5 cut(s) 124, 126, 168, 506, 903
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 6 cut(s) 122, 504, 686, 901, 903, 906
AclWI GGATC 2 cut(s) 25, 727
AcoI YGGCCR 2 cut(s) 301, 346
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 3 cut(s) 181, 356, 679
AfiI CCNNNNNNNGG 1 cut(s) 881
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 1 cut(s) 858
AjnI CCWGG 1 cut(s) 128
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 7 cut(s) 85, 247, 317, 337, 343, 604, 617
AluI AGCT 7 cut(s) 85, 247, 317, 337, 343, 604, 617
Alw26I GTCTC 3 cut(s) 73, 568, 752
AlwI GGATC 2 cut(s) 25, 727
Ama87I CYCGRG 2 cut(s) 743, 762
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 4 cut(s) 205, 301, 346, 501
ArsI GACNNNNNNTTYG 2 cut(s) 606, 638
AspLEI GCGC 4 cut(s) 126, 128, 168, 170
AspS9I GGNCC 1 cut(s) 428
AsuC2I CCSGG 2 cut(s) 763, 764
AsuHPI GGTGA 1 cut(s) 394
AvaI CYCGRG 2 cut(s) 743, 762
AvaII GGWCC 1 cut(s) 428
BanII GRGCYC 1 cut(s) 889
BccI CCATC 1 cut(s) 921
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 130
BcnI CCSGG 2 cut(s) 763, 764
BcoDI GTCTC 3 cut(s) 73, 568, 752
BfaI CTAG 1 cut(s) 272
BglII AGATCT 1 cut(s) 216
BisI GCNGC 4 cut(s) 504, 686, 904, 907
BlpI GCTNAGC 1 cut(s) 605
BlsI GCNGC 4 cut(s) 505, 687, 905, 908
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 3 cut(s) 130, 763, 764
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 2 cut(s) 743, 762
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 3 cut(s) 430, 465, 886
BmrFI CCNGG 3 cut(s) 130, 763, 764
BpmI CTGGAG 2 cut(s) 323, 903
Bpu10I CCTNAGC 1 cut(s) 150
Bpu1102I GCTNAGC 1 cut(s) 605
BpuMI CCSGG 2 cut(s) 763, 764
BsaAI YACGTR 1 cut(s) 560
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 5 cut(s) 129, 737, 762, 890, 901
BsaWI WCCGGW 3 cut(s) 91, 425, 542
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 1 cut(s) 881
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 405
BseAI TCCGGA 1 cut(s) 425
BseBI CCWGG 1 cut(s) 130
BseDI CCNNGG 5 cut(s) 129, 737, 762, 890, 901
BseGI GGATG 2 cut(s) 807, 932
BseLI CCNNNNNNNGG 1 cut(s) 881
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 3 cut(s) 542, 619, 839
BseNI ACTGG 1 cut(s) 717
BsePI GCGCGC 2 cut(s) 124, 166
BseRI GAGGAG 2 cut(s) 61, 122
BseYI CCCAGC 1 cut(s) 313
Bsh1236I CGCG 5 cut(s) 124, 126, 168, 506, 903
BshFI GGCC 4 cut(s) 207, 303, 348, 503
BsiHKCI CYCGRG 2 cut(s) 743, 762
BsiSI CCGG 5 cut(s) 92, 426, 461, 543, 763
BslI CCNNNNNNNGG 1 cut(s) 881
BsmAI GTCTC 3 cut(s) 73, 568, 752
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 4 cut(s) 207, 303, 348, 503
Bso31I GGTCTC 1 cut(s) 752
BsoBI CYCGRG 2 cut(s) 743, 762
Bsp1286I GDGCHC 1 cut(s) 889
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 5 cut(s) 30, 216, 719, 779, 809
Bsp1720I GCTNAGC 1 cut(s) 605
BspACI CCGC 6 cut(s) 122, 504, 686, 901, 903, 906
BspANI GGCC 4 cut(s) 207, 303, 348, 503
BspCNI CTCAG 3 cut(s) 543, 618, 838
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 5 cut(s) 124, 126, 168, 506, 903
BspLI GGNNCC 3 cut(s) 430, 465, 886
BspPI GGATC 2 cut(s) 25, 727
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 717
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 5 cut(s) 129, 737, 762, 890, 901
BssHII GCGCGC 2 cut(s) 124, 166
BssMI GATC 5 cut(s) 30, 216, 719, 779, 809
Bst2UI CCWGG 1 cut(s) 130
Bst4CI ACNGT 3 cut(s) 388, 644, 729
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 5 cut(s) 126, 147, 168, 515, 602
BstDEI CTNAG 5 cut(s) 150, 494, 551, 605, 825
BstDSI CCRYGG 2 cut(s) 890, 901
BstF5I GGATG 2 cut(s) 807, 932
BstFNI CGCG 5 cut(s) 124, 126, 168, 506, 903
BstHHI GCGC 4 cut(s) 126, 128, 168, 170
BstKTI GATC 5 cut(s) 33, 219, 722, 782, 812
BstMAI GTCTC 3 cut(s) 73, 568, 752
BstMBI GATC 5 cut(s) 30, 216, 719, 779, 809
BstMWI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
BstNI CCWGG 1 cut(s) 130
BstSCI CCNGG 3 cut(s) 128, 761, 762
BstUI CGCG 5 cut(s) 124, 126, 168, 506, 903
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 4 cut(s) 207, 303, 348, 503
BtgI CCRYGG 2 cut(s) 890, 901
BtsCI GGATG 2 cut(s) 807, 932
BtsIMutI CAGTG 1 cut(s) 734
Cac8I GCNNGC 5 cut(s) 126, 147, 168, 515, 602
CfoI GCGC 4 cut(s) 126, 128, 168, 170
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 428
Cfr42I CCGCGG 1 cut(s) 904
Cfr9I CCCGGG 1 cut(s) 762
CseI GACGC 1 cut(s) 67
Csp6I GTAC 3 cut(s) 180, 355, 678
CviAII CATG 3 cut(s) 37, 47, 65
CviQI GTAC 3 cut(s) 180, 355, 678
DdeI CTNAG 5 cut(s) 150, 494, 551, 605, 825
DpnI GATC 5 cut(s) 32, 218, 721, 781, 811
DpnII GATC 5 cut(s) 30, 216, 719, 779, 809
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 2 cut(s) 301, 346
Eco24I GRGCYC 1 cut(s) 889
Eco31I GGTCTC 1 cut(s) 752
Eco47I GGWCC 1 cut(s) 428
Eco88I CYCGRG 2 cut(s) 743, 762
EcoRII CCWGG 1 cut(s) 128
EcoT38I GRGCYC 1 cut(s) 889
FaeI CATG 3 cut(s) 40, 50, 68
FaiI YATR 9 cut(s) 38, 48, 66, 251, 525, 527, 578, 912, 918
FalI AAGNNNNNCTT 2 cut(s) 775, 807
FatI CATG 3 cut(s) 36, 46, 64
Fnu4HI GCNGC 4 cut(s) 504, 686, 904, 907
FokI GGATG 1 cut(s) 814
FriOI GRGCYC 1 cut(s) 889
Fsp4HI GCNGC 4 cut(s) 504, 686, 904, 907
FspBI CTAG 1 cut(s) 272
GlaI GCGC 4 cut(s) 125, 127, 167, 169
GluI GCNGC 4 cut(s) 504, 686, 904, 907
GsaI CCCAGC 1 cut(s) 317
GsuI CTGGAG 2 cut(s) 323, 903
HaeIII GGCC 4 cut(s) 207, 303, 348, 503
HapII CCGG 5 cut(s) 92, 426, 461, 543, 763
HgaI GACGC 1 cut(s) 67
HhaI GCGC 4 cut(s) 126, 128, 168, 170
Hin1II CATG 3 cut(s) 40, 50, 68
Hin6I GCGC 4 cut(s) 124, 126, 166, 168
HinP1I GCGC 4 cut(s) 124, 126, 166, 168
HinfI GANTC 5 cut(s) 287, 434, 539, 862, 871
HpaII CCGG 5 cut(s) 92, 426, 461, 543, 763
HphI GGTGA 1 cut(s) 394
Hpy188I TCNGA 7 cut(s) 202, 244, 286, 325, 439, 538, 568
Hpy188III TCNNGA 5 cut(s) 382, 426, 723, 783, 882
Hpy99I CGWCG 5 cut(s) 101, 104, 206, 572, 614
HpyAV CCTTC 2 cut(s) 406, 590
HpyCH4III ACNGT 3 cut(s) 388, 644, 729
HpyCH4IV ACGT 1 cut(s) 559
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
HpyF3I CTNAG 5 cut(s) 150, 494, 551, 605, 825
HpySE526I ACGT 1 cut(s) 559
Hsp92II CATG 3 cut(s) 40, 50, 68
HspAI GCGC 4 cut(s) 124, 126, 166, 168
Kpn2I TCCGGA 1 cut(s) 425
KspI CCGCGG 1 cut(s) 904
Kzo9I GATC 5 cut(s) 30, 216, 719, 779, 809
LmnI GCTCC 3 cut(s) 90, 342, 884
MaeI CTAG 1 cut(s) 272
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 3 cut(s) 49, 400, 690
MalI GATC 5 cut(s) 32, 218, 721, 781, 811
MboI GATC 5 cut(s) 30, 216, 719, 779, 809
MboII GAAGA 9 cut(s) 7, 226, 638, 641, 644, 783, 789, 819, 829
MfeI CAATTG 1 cut(s) 584
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 889
MluCI AATT 7 cut(s) 257, 475, 508, 584, 750, 832, 913
MlyI GAGTC 3 cut(s) 281, 871, 880
MmeI TCCRAC 6 cut(s) 112, 225, 309, 570, 591, 697
MroI TCCGGA 1 cut(s) 425
MseI TTAA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 903
MspI CCGG 5 cut(s) 92, 426, 461, 543, 763
MspR9I CCNGG 3 cut(s) 130, 763, 764
MunI CAATTG 1 cut(s) 584
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 1 cut(s) 130
MvnI CGCG 5 cut(s) 124, 126, 168, 506, 903
MwoI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
NciI CCSGG 2 cut(s) 763, 764
NdeII GATC 5 cut(s) 30, 216, 719, 779, 809
NlaIII CATG 3 cut(s) 40, 50, 68
NlaIV GGNNCC 3 cut(s) 430, 465, 886
NmeAIII GCCGAG 2 cut(s) 324, 762
NmuCI GTSAC 2 cut(s) 49, 400
PaeR7I CTCGAG 1 cut(s) 743
PauI GCGCGC 2 cut(s) 124, 166
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 434, 539
PflMI CCANNNNNTGG 1 cut(s) 881
PkrI GCNGC 4 cut(s) 505, 687, 905, 908
PleI GAGTC 3 cut(s) 281, 870, 879
PpsI GAGTC 3 cut(s) 281, 870, 879
Ppu21I YACGTR 1 cut(s) 560
Psp6I CCWGG 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 1 cut(s) 128
PspN4I GGNNCC 3 cut(s) 430, 465, 886
PspPI GGNCC 1 cut(s) 428
PspXI VCTCGAGB 1 cut(s) 743
PsuI RGATCY 1 cut(s) 216
PteI GCGCGC 2 cut(s) 124, 166
RsaI GTAC 3 cut(s) 181, 356, 679
RsaNI GTAC 3 cut(s) 180, 355, 678
SacII CCGCGG 1 cut(s) 904
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 4 cut(s) 504, 686, 904, 907
Sau3AI GATC 5 cut(s) 30, 216, 719, 779, 809
Sau96I GGNCC 1 cut(s) 428
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 3 cut(s) 281, 871, 880
ScrFI CCNGG 3 cut(s) 130, 763, 764
SduI GDGCHC 1 cut(s) 889
Sfr274I CTCGAG 1 cut(s) 743
Sfr303I CCGCGG 1 cut(s) 904
SgrBI CCGCGG 1 cut(s) 904
SinI GGWCC 1 cut(s) 428
SlaI CTCGAG 1 cut(s) 743
SmaI CCCGGG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 7 cut(s) 257, 475, 508, 584, 750, 832, 913
SsiI CCGC 6 cut(s) 122, 504, 686, 901, 903, 906
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 128, 761, 762
TaaI ACNGT 3 cut(s) 388, 644, 729
TaiI ACGT 1 cut(s) 562
TaqI TCGA 2 cut(s) 624, 744
TasI AATT 7 cut(s) 257, 475, 508, 584, 750, 832, 913
TatI WGTACW 1 cut(s) 354
TauI GCSGC 4 cut(s) 506, 688, 906, 909
TfiI GAWTC 2 cut(s) 434, 539
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 1 cut(s) 734
TseFI GTSAC 2 cut(s) 49, 400
Tsp45I GTSAC 2 cut(s) 49, 400
TspDTI ATGAA 4 cut(s) 35, 512, 651, 825
TspMI CCCGGG 1 cut(s) 762
TspRI CASTG 1 cut(s) 734
Van91I CCANNNNNTGG 1 cut(s) 881
VpaK11BI GGWCC 1 cut(s) 428
XhoI CTCGAG 1 cut(s) 743
XmaI CCCGGG 1 cut(s) 762
XspI CTAG 1 cut(s) 272
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.