Rw4G024490

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014075
Physical Location & Seq
Forward (+)
50341157 .. 50342103
947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024490.1

Sequence Viewer

Length: 891 bp
ATGGGTGTGAGGTTCTTCACTCTCCAAATGATCCCCATGTGTTTTCATGTCACGGGCAACCACCATGTCTCCTCTTGCGTCCAGCTACAACCGGACGACGATGAGGAGGAGTATCAACCACCGCGCGCCAAGGTACGTGGTAAGCGTGCTAAGATAAGTGGTAAGCGCGCGGTGGTTGGTACCATCAAGGTCGTTAAGTCCGACGGCCTTGTGAAGATCTACAACAAGCCGATTGATGTATCAGAGCTTATGAGGCAATTTCCCAAGCACCTAGTGTGTGCTTCCGACTCATTTTACATCGGCCAGAAAATCCCACACTTTTTCCAGACTGTGCTCTCCTTCGTCACCATTGCCTCGTTCGCCACCTCCGGACCCGAATCCGAACCCGAACAAAAGCCCTCGCCGGTTCCCTCCACCAATTCCAAGAATGCGTTTCTAAGAAAGGCCGCGAATTGCGAGCCTTTTCATATACAGAAATCGGAATCCGGTTGCCTGAGAATACGTGTCTCCGACGAGTTTATATCGCAATTGTTGGAGGAAGGCAAGCTCAGCGACGAAGCTAAAGTCGAAGAAGAAGATGAACATGGTAAGAGTTTTGAGCCAAACAACAGCAAAGTCTGTACCACGCCGCAGTTACAGAAAGACTACTCGCAACTGGTTGGATCAAGACAATGGAAGCCGAGGCTCGAGCCAATTAGAGAGACCCGGGAAACCAAGAGAAGATCAAGACTTTCTTCGTTTGGGATGAGAAGATCAAAGAAGAAATCTCAACCCAATTCATCAAAAGCAACACAGAGTTCAAAGGGTCCAAAGAGTCCAAAGATTGCTACTTTAGTAAAATCTGAGCACCATTCCAAAACCAAGATCAAGATCAAATCATCAAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

296

Amino Acids

33.4

Weight (kDa)

9.78

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 60 - 106 1.8e-11 PADRE domain
PADRE PF14009 107 - 234 5e-07 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 5 cut(s) 124, 126, 168, 170, 449
AccIII TCCGGA 1 cut(s) 368
AciI CCGC 4 cut(s) 122, 170, 447, 629
AclWI GGATC 2 cut(s) 25, 670
AcoI YGGCCR 1 cut(s) 301
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 3 cut(s) 135, 181, 622
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 1 cut(s) 801
AluBI AGCT 4 cut(s) 85, 247, 547, 560
AluI AGCT 4 cut(s) 85, 247, 547, 560
Alw21I GWGCWC 2 cut(s) 336, 849
Alw26I GTCTC 3 cut(s) 73, 511, 695
AlwI GGATC 2 cut(s) 25, 670
Ama87I CYCGRG 2 cut(s) 686, 705
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 3 cut(s) 205, 301, 444
ArsI GACNNNNNNTTYG 2 cut(s) 549, 581
Asp718I GGTACC 1 cut(s) 179
AspLEI GCGC 4 cut(s) 126, 128, 168, 170
AspS9I GGNCC 2 cut(s) 371, 806
AsuC2I CCSGG 2 cut(s) 706, 707
AsuHPI GGTGA 1 cut(s) 337
AvaI CYCGRG 2 cut(s) 686, 705
AvaII GGWCC 2 cut(s) 371, 806
BanI GGYRCC 1 cut(s) 179
Bbv12I GWGCWC 2 cut(s) 336, 849
BccI CCATC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 220
BcnI CCSGG 2 cut(s) 706, 707
BcoDI GTCTC 3 cut(s) 73, 511, 695
BfaI CTAG 1 cut(s) 272
BglII AGATCT 1 cut(s) 216
BisI GCNGC 2 cut(s) 447, 629
BlpI GCTNAGC 1 cut(s) 548
BlsI GCNGC 2 cut(s) 448, 630
Bme1390I CCNGG 2 cut(s) 706, 707
Bme18I GGWCC 2 cut(s) 371, 806
BmeT110I CYCGRG 2 cut(s) 686, 705
BmgT120I GGNCC 2 cut(s) 371, 806
BmiI GGNNCC 4 cut(s) 181, 373, 408, 807
BmrFI CCNGG 2 cut(s) 706, 707
Bpu1102I GCTNAGC 1 cut(s) 548
BpuMI CCSGG 2 cut(s) 706, 707
BsaAI YACGTR 2 cut(s) 137, 503
BsaBI GATNNNNATC 1 cut(s) 869
BsaI GGTCTC 1 cut(s) 695
BsaJI CCNNGG 3 cut(s) 129, 680, 705
BsaWI WCCGGW 3 cut(s) 91, 368, 485
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 660
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 869
BseAI TCCGGA 1 cut(s) 368
BseDI CCNNGG 3 cut(s) 129, 680, 705
BseGI GGATG 1 cut(s) 750
BseJI GATNNNNATC 1 cut(s) 869
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 3 cut(s) 485, 562, 834
BseNI ACTGG 1 cut(s) 660
BsePI GCGCGC 2 cut(s) 124, 166
BseRI GAGGAG 3 cut(s) 61, 119, 122
Bsh1236I CGCG 5 cut(s) 124, 126, 168, 170, 449
BshFI GGCC 3 cut(s) 207, 303, 446
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 2 cut(s) 336, 849
BsiHKCI CYCGRG 2 cut(s) 686, 705
BsiSI CCGG 5 cut(s) 92, 369, 404, 486, 706
BsmAI GTCTC 3 cut(s) 73, 511, 695
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 3 cut(s) 207, 303, 446
Bso31I GGTCTC 1 cut(s) 695
BsoBI CYCGRG 2 cut(s) 686, 705
Bsp1286I GDGCHC 2 cut(s) 336, 849
Bsp13I TCCGGA 1 cut(s) 368
Bsp143I GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
Bsp1720I GCTNAGC 1 cut(s) 548
BspACI CCGC 4 cut(s) 122, 170, 447, 629
BspANI GGCC 3 cut(s) 207, 303, 446
BspCNI CTCAG 3 cut(s) 486, 561, 835
BspEI TCCGGA 1 cut(s) 368
BspFNI CGCG 5 cut(s) 124, 126, 168, 170, 449
BspLI GGNNCC 4 cut(s) 181, 373, 408, 807
BspPI GGATC 2 cut(s) 25, 670
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 348
BsrFI RCCGGY 1 cut(s) 403
BsrI ACTGG 1 cut(s) 660
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 3 cut(s) 129, 680, 705
BssHII GCGCGC 2 cut(s) 124, 166
BssMI GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 331
BstBAI YACGTR 2 cut(s) 137, 503
BstC8I GCNNGC 5 cut(s) 126, 147, 168, 458, 545
BstDEI CTNAG 5 cut(s) 150, 437, 494, 548, 843
BstF5I GGATG 1 cut(s) 750
BstFNI CGCG 5 cut(s) 124, 126, 168, 170, 449
BstHHI GCGC 4 cut(s) 126, 128, 168, 170
BstKTI GATC 7 cut(s) 33, 219, 665, 725, 755, 867, 873
BstMAI GTCTC 3 cut(s) 73, 511, 695
BstMBI GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
BstMWI GCNNNNNNNGC 3 cut(s) 253, 359, 549
BstSCI CCNGG 2 cut(s) 704, 705
BstUI CGCG 5 cut(s) 124, 126, 168, 170, 449
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 3 cut(s) 207, 303, 446
BtsCI GGATG 1 cut(s) 750
Cac8I GCNNGC 5 cut(s) 126, 147, 168, 458, 545
CfoI GCGC 4 cut(s) 126, 128, 168, 170
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 371, 806
Cfr9I CCCGGG 1 cut(s) 705
CseI GACGC 1 cut(s) 67
Csp6I GTAC 3 cut(s) 134, 180, 621
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 4 cut(s) 37, 47, 65, 584
CviQI GTAC 3 cut(s) 134, 180, 621
DdeI CTNAG 5 cut(s) 150, 437, 494, 548, 843
DpnI GATC 7 cut(s) 32, 218, 664, 724, 754, 866, 872
DpnII GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 695
Eco47I GGWCC 2 cut(s) 371, 806
Eco88I CYCGRG 2 cut(s) 686, 705
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 4 cut(s) 40, 50, 68, 587
FaiI YATR 8 cut(s) 38, 48, 66, 251, 468, 470, 521, 585
FalI AAGNNNNNCTT 2 cut(s) 718, 750
FatI CATG 4 cut(s) 36, 46, 64, 583
Fnu4HI GCNGC 2 cut(s) 447, 629
FokI GGATG 1 cut(s) 757
Fsp4HI GCNGC 2 cut(s) 447, 629
FspBI CTAG 1 cut(s) 272
GlaI GCGC 4 cut(s) 125, 127, 167, 169
GluI GCNGC 2 cut(s) 447, 629
HaeIII GGCC 3 cut(s) 207, 303, 446
HapII CCGG 5 cut(s) 92, 369, 404, 486, 706
HgaI GACGC 1 cut(s) 67
HhaI GCGC 4 cut(s) 126, 128, 168, 170
Hin1II CATG 4 cut(s) 40, 50, 68, 587
Hin6I GCGC 4 cut(s) 124, 126, 166, 168
HinP1I GCGC 4 cut(s) 124, 126, 166, 168
HinfI GANTC 4 cut(s) 287, 377, 482, 814
HpaII CCGG 5 cut(s) 92, 369, 404, 486, 706
HphI GGTGA 1 cut(s) 337
Hpy188I TCNGA 7 cut(s) 202, 244, 286, 382, 481, 511, 844
Hpy188III TCNNGA 5 cut(s) 325, 369, 666, 726, 868
Hpy99I CGWCG 4 cut(s) 101, 206, 515, 557
HpyAV CCTTC 2 cut(s) 349, 533
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 2 cut(s) 136, 502
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 359, 549
HpyF3I CTNAG 5 cut(s) 150, 437, 494, 548, 843
HpySE526I ACGT 2 cut(s) 136, 502
Hsp92II CATG 4 cut(s) 40, 50, 68, 587
HspAI GCGC 4 cut(s) 124, 126, 166, 168
Kpn2I TCCGGA 1 cut(s) 368
KpnI GGTACC 1 cut(s) 183
Kzo9I GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
MaeI CTAG 1 cut(s) 272
MaeII ACGT 2 cut(s) 136, 502
MaeIII GTNAC 3 cut(s) 49, 343, 633
MalI GATC 7 cut(s) 32, 218, 664, 724, 754, 866, 872
MboI GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
MboII GAAGA 9 cut(s) 7, 226, 581, 584, 587, 726, 732, 762, 772
MfeI CAATTG 1 cut(s) 527
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 336, 849
MluCI AATT 6 cut(s) 257, 418, 451, 527, 693, 775
MlyI GAGTC 2 cut(s) 281, 823
MmeI TCCRAC 5 cut(s) 225, 309, 513, 534, 640
MroI TCCGGA 1 cut(s) 368
MseI TTAA 1 cut(s) 195
MspI CCGG 5 cut(s) 92, 369, 404, 486, 706
MspR9I CCNGG 2 cut(s) 706, 707
MunI CAATTG 1 cut(s) 527
Mva1269I GAATGC 1 cut(s) 433
MvnI CGCG 5 cut(s) 124, 126, 168, 170, 449
MwoI GCNNNNNNNGC 3 cut(s) 253, 359, 549
NciI CCSGG 2 cut(s) 706, 707
NdeII GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
NlaIII CATG 4 cut(s) 40, 50, 68, 587
NlaIV GGNNCC 4 cut(s) 181, 373, 408, 807
NmeAIII GCCGAG 1 cut(s) 705
NmuCI GTSAC 2 cut(s) 49, 343
PaeR7I CTCGAG 1 cut(s) 686
PauI GCGCGC 2 cut(s) 124, 166
PcsI WCGNNNNNNNCGW 2 cut(s) 142, 198
PctI GAATGC 1 cut(s) 433
PfeI GAWTC 2 cut(s) 377, 482
PkrI GCNGC 2 cut(s) 448, 630
PleI GAGTC 2 cut(s) 281, 822
PpsI GAGTC 2 cut(s) 281, 822
Ppu21I YACGTR 2 cut(s) 137, 503
PspN4I GGNNCC 4 cut(s) 181, 373, 408, 807
PspPI GGNCC 2 cut(s) 371, 806
PspXI VCTCGAGB 1 cut(s) 686
PsuI RGATCY 1 cut(s) 216
PteI GCGCGC 2 cut(s) 124, 166
RsaI GTAC 3 cut(s) 135, 181, 622
RsaNI GTAC 3 cut(s) 134, 180, 621
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 2 cut(s) 447, 629
Sau3AI GATC 7 cut(s) 30, 216, 662, 722, 752, 864, 870
Sau96I GGNCC 2 cut(s) 371, 806
SchI GAGTC 2 cut(s) 281, 823
ScrFI CCNGG 2 cut(s) 706, 707
SduI GDGCHC 2 cut(s) 336, 849
Sfr274I CTCGAG 1 cut(s) 686
SinI GGWCC 2 cut(s) 371, 806
SlaI CTCGAG 1 cut(s) 686
SmaI CCCGGG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 6 cut(s) 257, 418, 451, 527, 693, 775
SsiI CCGC 4 cut(s) 122, 170, 447, 629
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 704, 705
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 2 cut(s) 139, 505
TaqI TCGA 2 cut(s) 567, 687
TasI AATT 6 cut(s) 257, 418, 451, 527, 693, 775
TauI GCSGC 2 cut(s) 449, 631
TfiI GAWTC 2 cut(s) 377, 482
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseFI GTSAC 2 cut(s) 49, 343
Tsp45I GTSAC 2 cut(s) 49, 343
TspDTI ATGAA 4 cut(s) 35, 455, 594, 768
TspMI CCCGGG 1 cut(s) 705
VpaK11BI GGWCC 2 cut(s) 371, 806
XhoI CTCGAG 1 cut(s) 686
XmaI CCCGGG 1 cut(s) 705
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.