MD13G1134300.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014075
Physical Location & Seq
Reverse (-)
10287309 .. 10288094
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1134300.v1.1.491

Sequence Viewer

Length: 786 bp
ATGAAGTTGCGGTTCTTCGCACACCAAATGGTGCCGTGGTGCTTCCAATTAATGGGCAGCCACCACGTATCTCGCGCGCAGCTCCGTTCCAAAGACAAAGCCCGCCCGATCGGCACCATCAAGCTCATCAAATCGGACGGGCTTGTGTTGGTCTACCACCAACCAATCCACGTTTCGGAAGTCACGTCGGAGTTTCCGAAACACCAAGTGTGCCGGTCCGATTCTTTTTACATCGGGCAGAAGATCCCGGCACTGTCGGAGGATGACCAGCTCCAGCTCGGCCACAAGTACTTTCTCCTCCCCCAGCAGTTTTTCCAATCCGTGCTTTCGTTCGTCACCATTGCTTCCTTCAGCAACACCAAAGACTCGTCCAGAAACGCGTTTCTTAGAAAGGCCGCGAGCTGCGAGCCGTTCGATATACAGAAAACGCCTTCTGGGTGTTTGAGAATACGCGTTTCGGATGAGTTTATATCGCAGCTGCTGGAGGAAAAAAAGCTCAATGAGGAAGAGGACACGGCGGCGACGGAAGCCAAGAGCAGGGTCTGCACAACGGCTCAGCTGCAGAAGGACTACACGCAGCTTGTTGGGTCGCGGCTGTGGAAGCCGAGGCTCGAGCCCATCAGAGAGGTTCGGGAGAAGAGAAGAAAGCTTTCTTCTTTTGGGATGAGAAGATCTTGCAAGAAGAAATCTCAGCTGAATTCATCCAAAGCTTCTCAGAAGCAAAAGAGTAGTTCTGCTAATGGTTCTTCCAAAGCCAAGATCAAGATTAAATCATCAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

262

Amino Acids

29.83

Weight (kDa)

10.04

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 18 - 209 1.6e-33 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 31, 113
AccB7I CCANNNNNTGG 1 cut(s) 52
AccI GTMKAC 1 cut(s) 153
AccII CGCG 6 cut(s) 75, 77, 380, 398, 453, 592
AciI CCGC 5 cut(s) 10, 103, 396, 518, 592
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 334
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 3 cut(s) 52, 175, 537
AflIII ACRYGT 2 cut(s) 378, 451
AjiI CACGTC 1 cut(s) 186
AjuI GAANNNNNNNTTGG 2 cut(s) 157, 189
AlwI GGATC 1 cut(s) 238
AlwNI CAGNNNCTG 2 cut(s) 481, 543
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 2 cut(s) 280, 393
ApeKI GCWGC 7 cut(s) 57, 79, 402, 475, 478, 559, 577
ApoI RAATTY 1 cut(s) 697
AseI ATTAAT 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 649
AspLEI GCGC 2 cut(s) 77, 79
AspS9I GGNCC 1 cut(s) 216
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 611
AvaII GGWCC 1 cut(s) 216
BanI GGYRCC 2 cut(s) 31, 113
BanII GRGCYC 1 cut(s) 618
BbvI GCAGC 7 cut(s) 69, 91, 389, 465, 487, 546, 589
BccI CCATC 2 cut(s) 125, 626
BceAI ACGGC 4 cut(s) 19, 394, 531, 567
BcnI CCSGG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 560
BglI GCCNNNNNGGC 1 cut(s) 111
BglII AGATCT 1 cut(s) 671
BlpI GCTNAGC 1 cut(s) 555
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 216
BmeT110I CYCGRG 1 cut(s) 611
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 216
BmiI GGNNCC 2 cut(s) 33, 115
BmrFI CCNGG 1 cut(s) 248
BpmI CTGGAG 2 cut(s) 257, 503
Bpu1102I GCTNAGC 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 35, 605
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 175, 537
Bse118I RCCGGY 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 35, 605
BseGI GGATG 4 cut(s) 268, 466, 669, 701
BseLI CCNNNNNNNGG 3 cut(s) 52, 175, 537
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 3 cut(s) 569, 704, 728
BsePI GCGCGC 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 7 cut(s) 69, 91, 389, 465, 487, 546, 589
BseYI CCCAGC 1 cut(s) 303
BsgI GTGCAG 1 cut(s) 529
Bsh1236I CGCG 6 cut(s) 75, 77, 380, 398, 453, 592
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 282, 395
BshNI GGYRCC 2 cut(s) 31, 113
BsiEI CGRYCG 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 2 cut(s) 214, 248
BslI CCNNNNNNNGG 3 cut(s) 52, 175, 537
BsnI GGCC 2 cut(s) 282, 395
BsoBI CYCGRG 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 618
Bsp143I GATC 4 cut(s) 108, 243, 671, 759
Bsp1720I GCTNAGC 1 cut(s) 555
BspACI CCGC 5 cut(s) 10, 103, 396, 518, 592
BspANI GGCC 2 cut(s) 282, 395
BspCNI CTCAG 3 cut(s) 568, 703, 727
BspFNI CGCG 6 cut(s) 75, 77, 380, 398, 453, 592
BspLI GGNNCC 2 cut(s) 33, 115
BspMAI CTGCAG 1 cut(s) 564
BspPI GGATC 1 cut(s) 238
BspT107I GGYRCC 2 cut(s) 31, 113
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 213
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 2 cut(s) 35, 605
BssHII GCGCGC 1 cut(s) 75
BssMI GATC 4 cut(s) 108, 243, 671, 759
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 2 cut(s) 501, 632
BstAPI GCANNNNNTGC 1 cut(s) 543
BstBAI YACGTR 1 cut(s) 67
BstC8I GCNNGC 4 cut(s) 77, 103, 400, 407
BstDEI CTNAG 4 cut(s) 386, 555, 690, 714
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 4 cut(s) 268, 466, 669, 701
BstFNI CGCG 6 cut(s) 75, 77, 380, 398, 453, 592
BstHHI GCGC 2 cut(s) 77, 79
BstKTI GATC 4 cut(s) 111, 246, 674, 762
BstMBI GATC 4 cut(s) 108, 243, 671, 759
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 4 cut(s) 111, 527, 543, 601
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 560
BstUI CGCG 6 cut(s) 75, 77, 380, 398, 453, 592
BstV1I GCAGC 7 cut(s) 69, 91, 389, 465, 487, 546, 589
BstX2I RGATCY 2 cut(s) 243, 671
BstYI RGATCY 2 cut(s) 243, 671
BsuRI GGCC 2 cut(s) 282, 395
BtgI CCRYGG 1 cut(s) 35
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 4 cut(s) 268, 466, 669, 701
BtsIMutI CAGTG 1 cut(s) 251
Cac8I GCNNGC 4 cut(s) 77, 103, 400, 407
CaiI CAGNNNCTG 2 cut(s) 481, 543
CfoI GCGC 2 cut(s) 77, 79
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 216
CpoI CGGWCCG 1 cut(s) 216
Csp6I GTAC 1 cut(s) 289
CspI CGGWCCG 1 cut(s) 216
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 4 cut(s) 386, 555, 690, 714
DpnI GATC 4 cut(s) 110, 245, 673, 761
DpnII GATC 4 cut(s) 108, 243, 671, 759
DraIII CACNNNGTG 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 2 cut(s) 501, 632
EarI CTCTTC 2 cut(s) 501, 632
Eco24I GRGCYC 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 697
EcoT38I GRGCYC 1 cut(s) 618
FaiI YATR 2 cut(s) 419, 470
FauI CCCGC 1 cut(s) 110
FblI GTMKAC 1 cut(s) 153
FokI GGATG 4 cut(s) 275, 473, 676, 688
FriOI GRGCYC 1 cut(s) 618
GlaI GCGC 2 cut(s) 76, 78
GsaI CCCAGC 1 cut(s) 307
GsuI CTGGAG 2 cut(s) 257, 503
HaeIII GGCC 2 cut(s) 282, 395
HapII CCGG 2 cut(s) 214, 248
HhaI GCGC 2 cut(s) 77, 79
Hin6I GCGC 2 cut(s) 75, 77
HinP1I GCGC 2 cut(s) 75, 77
HindIII AAGCTT 2 cut(s) 647, 708
HinfI GANTC 2 cut(s) 221, 365
HpaII CCGG 2 cut(s) 214, 248
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 9 cut(s) 136, 178, 190, 198, 220, 259, 460, 623, 717
Hpy188III TCNNGA 4 cut(s) 372, 632, 763, 777
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 2 cut(s) 190, 526
HpyAV CCTTC 3 cut(s) 358, 441, 559
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 3 cut(s) 66, 171, 185
HpyCH4V TGCA 3 cut(s) 546, 562, 678
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 527, 543, 601
HpyF3I CTNAG 4 cut(s) 386, 555, 690, 714
HpySE526I ACGT 3 cut(s) 66, 171, 185
HspAI GCGC 2 cut(s) 75, 77
Kzo9I GATC 4 cut(s) 108, 243, 671, 759
LmnI GCTCC 2 cut(s) 87, 276
LpnPI CCDG 9 cut(s) 227, 261, 281, 287, 317, 385, 420, 467, 523
Lsp1109I GCAGC 7 cut(s) 69, 91, 389, 465, 487, 546, 589
MaeII ACGT 3 cut(s) 66, 171, 185
MaeIII GTNAC 2 cut(s) 181, 334
MalI GATC 4 cut(s) 110, 245, 673, 761
MboI GATC 4 cut(s) 108, 243, 671, 759
MboII GAAGA 9 cut(s) 7, 253, 518, 645, 649, 654, 681, 694, 738
MflI RGATCY 2 cut(s) 243, 671
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 2 cut(s) 47, 697
MluI ACGCGT 2 cut(s) 378, 451
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 168, 237
MnlI CCTC 7 cut(s) 253, 308, 478, 496, 502, 600, 619
MroXI GAANNNNTTC 1 cut(s) 649
MseI TTAA 2 cut(s) 50, 768
MspA1I CMGCKG 3 cut(s) 478, 559, 694
MspI CCGG 2 cut(s) 214, 248
MspR9I CCNGG 1 cut(s) 248
MvnI CGCG 6 cut(s) 75, 77, 380, 398, 453, 592
MwoI GCNNNNNNNGC 4 cut(s) 111, 527, 543, 601
NciI CCSGG 1 cut(s) 248
NdeII GATC 4 cut(s) 108, 243, 671, 759
NlaIV GGNNCC 2 cut(s) 33, 115
NmeAIII GCCGAG 2 cut(s) 258, 630
NmuCI GTSAC 2 cut(s) 181, 334
PaeR7I CTCGAG 1 cut(s) 611
PauI GCGCGC 1 cut(s) 75
PcsI WCGNNNNNNNCGW 2 cut(s) 182, 194
PdmI GAANNNNTTC 1 cut(s) 649
PfeI GAWTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 52
Ple19I CGATCG 1 cut(s) 111
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
Ppu21I YACGTR 1 cut(s) 67
PshBI ATTAAT 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 33, 115
PspPI GGNCC 1 cut(s) 216
PspXI VCTCGAGB 1 cut(s) 611
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 2 cut(s) 481, 543
PsuI RGATCY 2 cut(s) 243, 671
PteI GCGCGC 1 cut(s) 75
PvuI CGATCG 1 cut(s) 111
PvuII CAGCTG 3 cut(s) 478, 559, 694
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
Rsr2I CGGWCCG 1 cut(s) 216
RsrII CGGWCCG 1 cut(s) 216
SaqAI TTAA 2 cut(s) 50, 768
Sau3AI GATC 4 cut(s) 108, 243, 671, 759
Sau96I GGNCC 1 cut(s) 216
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 1 cut(s) 618
SfcI CTRYAG 1 cut(s) 560
Sfr274I CTCGAG 1 cut(s) 611
SinI GGWCC 1 cut(s) 216
SlaI CTCGAG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 2 cut(s) 47, 697
SsiI CCGC 5 cut(s) 10, 103, 396, 518, 592
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 3 cut(s) 69, 174, 188
TaqI TCGA 2 cut(s) 414, 612
TasI AATT 2 cut(s) 47, 697
TatI WGTACW 1 cut(s) 288
TauI GCSGC 3 cut(s) 398, 521, 595
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 50, 768
Tru9I TTAA 2 cut(s) 50, 768
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 2 cut(s) 181, 334
TseI GCWGC 7 cut(s) 57, 79, 402, 475, 478, 559, 577
Tsp45I GTSAC 2 cut(s) 181, 334
TspDTI ATGAA 2 cut(s) 17, 690
TspGWI ACGGA 3 cut(s) 74, 310, 539
TspRI CASTG 1 cut(s) 258
Van91I CCANNNNNTGG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 216
VspI ATTAAT 1 cut(s) 50
XapI RAATTY 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 611
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 649
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.