Rroxscaffold_5G00370040

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014075
Physical Location & Seq
Forward (+)
51761086 .. 51761988
903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370040.1

Sequence Viewer

Length: 903 bp
ATGGGTGTGAGGTTCTTCACTCTCCAAATGATCCCCATGTGTTTTCATATCACGGGCAACCATCATGTCTCCTCTTGCGTCCAGCTCCAACCGGACGACGATGAGGAGGAGTATCAACCACCGCGAGCCAAGGTAGGTGGTAAGCGTGCTAAGGTAGGTGGTAAGCGCGCGGTCGTTGGTACCATCAAGGTCGTTAAGTCCGACGGCCTTGTGAAGATATACAACAAGCCGATTGATGTATCAGAGCTTATGAGGCAATTTCCCAAGCACCTAGTGTGTGCTTCCGACTCATTTTACATCGGCCAGAAAATCCCAGCTCTTTCGGAACACGACCAGCTCCAGCTCGGCCGAAAGTACTTTCTGCTACCGAAGCACTTTTTCCAGTCTGTGCTCTCCTTCGTCACCATTGCCTCGTTCGCCACCTCCGGACCCGAATCCGAACCCGAAGAAAAGCCCTCGCCGGTTCCCTCCACCAATTCCAAGAATGCGTTTCTAAGAAAGGCCGCGAATTGCGACCCTTTTCATATACAGAAATCGGAATCCGGTTGCCTGAGAATACGTGTCTCGGACGAGTTTATATCGCAATTGTTGGAGGAAGGCAAGCTCAGCGACGAAGCTAAAGTCGAAGAAGAAGATGAACACGGTAAGAGTTTTGAGCCAAACAACAGCAAAGTCTGTACCACTCCGCAGTTACAGAAAGACTACTCGCAACTGGTTGGATCAAGACAATGGAAGCCGAGGCTCGAGCCAATTAGAGAGACCCGGGAAACCAAAGAGAAGATCAAGACTTTCTTCGTTTGGGATGAGAAGATCAAAGAAGAAATCTCAACCCAATTCATCAAAAGCAACACAGAGTTCAAAGAGTTCAAAGAGTCCAAAGATTGCTACTCTAGTAAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

300

Amino Acids

34.24

Weight (kDa)

8.06

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 60 - 253 1e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 4 cut(s) 124, 168, 170, 506
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 4 cut(s) 122, 170, 504, 686
AclWI GGATC 2 cut(s) 25, 727
AcoI YGGCCR 2 cut(s) 301, 346
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 3 cut(s) 181, 356, 679
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 2 cut(s) 859, 868
AluBI AGCT 7 cut(s) 85, 247, 317, 337, 343, 604, 617
AluI AGCT 7 cut(s) 85, 247, 317, 337, 343, 604, 617
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 3 cut(s) 73, 568, 752
AlwI GGATC 2 cut(s) 25, 727
Ama87I CYCGRG 2 cut(s) 743, 762
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 4 cut(s) 205, 301, 346, 501
ArsI GACNNNNNNTTYG 2 cut(s) 606, 638
Asp718I GGTACC 1 cut(s) 179
AspLEI GCGC 2 cut(s) 168, 170
AspS9I GGNCC 1 cut(s) 428
AsuC2I CCSGG 2 cut(s) 763, 764
AsuHPI GGTGA 1 cut(s) 394
AvaI CYCGRG 2 cut(s) 743, 762
AvaII GGWCC 1 cut(s) 428
BanI GGYRCC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 393
BccI CCATC 2 cut(s) 69, 191
BceAI ACGGC 1 cut(s) 220
BcnI CCSGG 2 cut(s) 763, 764
BcoDI GTCTC 3 cut(s) 73, 568, 752
BfaI CTAG 2 cut(s) 272, 891
BisI GCNGC 1 cut(s) 504
BlpI GCTNAGC 1 cut(s) 605
BlsI GCNGC 1 cut(s) 505
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 763, 764
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 2 cut(s) 743, 762
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 3 cut(s) 181, 430, 465
BmrFI CCNGG 2 cut(s) 763, 764
BpmI CTGGAG 1 cut(s) 323
Bpu10I CCTNAGC 1 cut(s) 150
Bpu1102I GCTNAGC 1 cut(s) 605
BpuMI CCSGG 2 cut(s) 763, 764
BsaAI YACGTR 1 cut(s) 560
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 3 cut(s) 129, 737, 762
BsaWI WCCGGW 3 cut(s) 91, 425, 542
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 382, 717
Bse3DI GCAATG 1 cut(s) 405
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 3 cut(s) 129, 737, 762
BseGI GGATG 1 cut(s) 808
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 2 cut(s) 542, 619
BseNI ACTGG 2 cut(s) 382, 717
BsePI GCGCGC 1 cut(s) 166
BseRI GAGGAG 3 cut(s) 61, 119, 122
BseX3I CGGCCG 1 cut(s) 346
BseYI CCCAGC 1 cut(s) 313
Bsh1236I CGCG 4 cut(s) 124, 168, 170, 506
Bsh1285I CGRYCG 2 cut(s) 174, 349
BshFI GGCC 4 cut(s) 207, 303, 348, 503
BshNI GGYRCC 1 cut(s) 179
BsiEI CGRYCG 2 cut(s) 174, 349
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 2 cut(s) 743, 762
BsiSI CCGG 5 cut(s) 92, 426, 461, 543, 763
BsmAI GTCTC 3 cut(s) 73, 568, 752
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 4 cut(s) 207, 303, 348, 503
Bso31I GGTCTC 1 cut(s) 752
BsoBI CYCGRG 2 cut(s) 743, 762
Bsp1286I GDGCHC 1 cut(s) 393
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 4 cut(s) 30, 719, 780, 810
Bsp1720I GCTNAGC 1 cut(s) 605
BspACI CCGC 4 cut(s) 122, 170, 504, 686
BspANI GGCC 4 cut(s) 207, 303, 348, 503
BspCNI CTCAG 2 cut(s) 543, 618
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 4 cut(s) 124, 168, 170, 506
BspLI GGNNCC 3 cut(s) 181, 430, 465
BspPI GGATC 2 cut(s) 25, 727
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 2 cut(s) 382, 717
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 3 cut(s) 129, 737, 762
BssHII GCGCGC 1 cut(s) 166
BssMI GATC 4 cut(s) 30, 719, 780, 810
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 644
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 126, 147, 168, 602
BstDEI CTNAG 4 cut(s) 150, 494, 551, 605
BstF5I GGATG 1 cut(s) 808
BstFNI CGCG 4 cut(s) 124, 168, 170, 506
BstHHI GCGC 2 cut(s) 168, 170
BstKTI GATC 4 cut(s) 33, 722, 783, 813
BstMAI GTCTC 3 cut(s) 73, 568, 752
BstMBI GATC 4 cut(s) 30, 719, 780, 810
BstMCI CGRYCG 2 cut(s) 174, 349
BstMWI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
BstSCI CCNGG 2 cut(s) 761, 762
BstUI CGCG 4 cut(s) 124, 168, 170, 506
BstZI CGGCCG 1 cut(s) 346
BsuRI GGCC 4 cut(s) 207, 303, 348, 503
BtsCI GGATG 1 cut(s) 808
Cac8I GCNNGC 4 cut(s) 126, 147, 168, 602
CfoI GCGC 2 cut(s) 168, 170
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 428
Cfr9I CCCGGG 1 cut(s) 762
CseI GACGC 1 cut(s) 67
Csp6I GTAC 3 cut(s) 180, 355, 678
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 2 cut(s) 37, 65
CviQI GTAC 3 cut(s) 180, 355, 678
DdeI CTNAG 4 cut(s) 150, 494, 551, 605
DpnI GATC 4 cut(s) 32, 721, 782, 812
DpnII GATC 4 cut(s) 30, 719, 780, 810
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 2 cut(s) 301, 346
EagI CGGCCG 1 cut(s) 346
EclXI CGGCCG 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 752
Eco47I GGWCC 1 cut(s) 428
Eco52I CGGCCG 1 cut(s) 346
Eco88I CYCGRG 2 cut(s) 743, 762
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 2 cut(s) 40, 68
FaiI YATR 8 cut(s) 38, 48, 66, 220, 251, 525, 527, 578
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FatI CATG 2 cut(s) 36, 64
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 1 cut(s) 815
Fsp4HI GCNGC 1 cut(s) 504
FspBI CTAG 2 cut(s) 272, 891
GlaI GCGC 2 cut(s) 167, 169
GluI GCNGC 1 cut(s) 504
GsaI CCCAGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 323
HaeIII GGCC 4 cut(s) 207, 303, 348, 503
HapII CCGG 5 cut(s) 92, 426, 461, 543, 763
HgaI GACGC 1 cut(s) 67
HhaI GCGC 2 cut(s) 168, 170
Hin1II CATG 2 cut(s) 40, 68
Hin6I GCGC 2 cut(s) 166, 168
HinP1I GCGC 2 cut(s) 166, 168
HinfI GANTC 4 cut(s) 287, 434, 539, 872
HpaII CCGG 5 cut(s) 92, 426, 461, 543, 763
HphI GGTGA 1 cut(s) 394
Hpy188I TCNGA 8 cut(s) 202, 244, 286, 325, 439, 538, 568, 902
Hpy188III TCNNGA 3 cut(s) 426, 723, 784
Hpy99I CGWCG 3 cut(s) 101, 206, 614
HpyAV CCTTC 2 cut(s) 406, 590
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4IV ACGT 1 cut(s) 559
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
HpyF3I CTNAG 4 cut(s) 150, 494, 551, 605
HpySE526I ACGT 1 cut(s) 559
Hsp92II CATG 2 cut(s) 40, 68
HspAI GCGC 2 cut(s) 166, 168
Kpn2I TCCGGA 1 cut(s) 425
KpnI GGTACC 1 cut(s) 183
Kzo9I GATC 4 cut(s) 30, 719, 780, 810
LmnI GCTCC 2 cut(s) 90, 342
MaeI CTAG 2 cut(s) 272, 891
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 2 cut(s) 400, 690
MalI GATC 4 cut(s) 32, 721, 782, 812
MboI GATC 4 cut(s) 30, 719, 780, 810
MfeI CAATTG 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 393
MluCI AATT 6 cut(s) 257, 475, 508, 584, 750, 833
MlyI GAGTC 2 cut(s) 281, 881
MmeI TCCRAC 5 cut(s) 112, 225, 309, 570, 697
MroI TCCGGA 1 cut(s) 425
MseI TTAA 1 cut(s) 195
MspI CCGG 5 cut(s) 92, 426, 461, 543, 763
MspR9I CCNGG 2 cut(s) 763, 764
MunI CAATTG 1 cut(s) 584
Mva1269I GAATGC 1 cut(s) 490
MvnI CGCG 4 cut(s) 124, 168, 170, 506
MwoI GCNNNNNNNGC 4 cut(s) 253, 370, 416, 606
NciI CCSGG 2 cut(s) 763, 764
NdeII GATC 4 cut(s) 30, 719, 780, 810
NlaIII CATG 2 cut(s) 40, 68
NlaIV GGNNCC 3 cut(s) 181, 430, 465
NmeAIII GCCGAG 2 cut(s) 324, 762
NmuCI GTSAC 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 743
PauI GCGCGC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 434, 539
PkrI GCNGC 1 cut(s) 505
PleI GAGTC 2 cut(s) 281, 880
PpsI GAGTC 2 cut(s) 281, 880
Ppu21I YACGTR 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 181, 430, 465
PspPI GGNCC 1 cut(s) 428
PspXI VCTCGAGB 1 cut(s) 743
PteI GCGCGC 1 cut(s) 166
RsaI GTAC 3 cut(s) 181, 356, 679
RsaNI GTAC 3 cut(s) 180, 355, 678
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 4 cut(s) 30, 719, 780, 810
Sau96I GGNCC 1 cut(s) 428
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 2 cut(s) 281, 881
ScrFI CCNGG 2 cut(s) 763, 764
SduI GDGCHC 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 743
SinI GGWCC 1 cut(s) 428
SlaI CTCGAG 1 cut(s) 743
SmaI CCCGGG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 6 cut(s) 257, 475, 508, 584, 750, 833
SsiI CCGC 4 cut(s) 122, 170, 504, 686
SspMI CTAG 2 cut(s) 272, 891
StyD4I CCNGG 2 cut(s) 761, 762
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 644
TaiI ACGT 1 cut(s) 562
TaqI TCGA 2 cut(s) 624, 744
TasI AATT 6 cut(s) 257, 475, 508, 584, 750, 833
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 2 cut(s) 434, 539
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseFI GTSAC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 4 cut(s) 35, 512, 651, 826
TspMI CCCGGG 1 cut(s) 762
VpaK11BI GGWCC 1 cut(s) 428
XhoI CTCGAG 1 cut(s) 743
XmaI CCCGGG 1 cut(s) 762
XspI CTAG 2 cut(s) 272, 891
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.