Prupe.1G211200_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014075
Physical Location & Seq
Forward (+)
22470236 .. 22471553
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G211200.1

Sequence Viewer

Length: 801 bp
ATGAGGTTGCGATTTTTCACTCTCCAGATGATCCCATGGTGTTTCCAGTTGATGGGCAACCACCATCTCTCTCGCGTGCAGCTTCGTCACACCGATCAACAGCGCCAGGTTGTGGGTACCATCAAGCTCGTCAAATCCGACGGCCTGGTTTTGATCTACCACAGCCCAATCTACGTCTCCGAGCTCATGATGGAGTTTCCGAAACACCTTGTGTGCCGGTCCGATTCGTTCTATATCGGACAGAAGATCCCGGCTCTCTCTGAAGACGACCAGCTTCAGCTCGGCCACAAGTACTTTCTGCTACCTCAACACTTGTTCCAGTCGGTGCTTTCTTTCGTGACCATTGCTTCGTTCAATACCAAAGACTCGTCAAAAACCGCGTTTCTAAGGAAGGCTGCGAATTGCGAGCCGTTTGATATTCAGAAAACGCCATCTGGGTGCTTGAGGATACGCGTTTCCGACGAGTTTATATCCCAGATGTTGGAGGAAGGGAAGCTCAACGAGGAAGCGGCGAATAACAATGATGAGGCAAATGATTTTGCCAAAGCCAAGAGCAGGGTTTGCACGACGGCACAGTTGCAGAAAGACTACACGCAGCTTGTTGGGTCGCGGCAATGGAAGCCGAGGCTTGAGCCCATCAGAGAGACGAGGGAGAAGAGCAGAAGGCTGTCTTCGTTTGGGATGAGAAGATCTTCCAAGAAGAAATCTCAACCCAACTCATTAAAGGCGTCCCAGAACCAAAAGAGTTCTAGTACTGCTACTTCCAAAGCCAAGATCAAGATCAAACCATCAAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

267

Amino Acids

30.59

Weight (kDa)

9.96

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28190
fragaria_vesca FvH4_4g22140
malus_domestica MD13G1134300.v1.1 MD16G1144900.v1.1
prunus_persica Prupe.1G211200_v2.0.a1
pyrus_communis pycom13g11710 pycom16g11450
rosa_chinensis RchiOBHm_Chr4g0428381
rosa_laevigata RLG00000007156
rosa_multiflora Rmu_sc0004698.1_g000010
rosa_roxburghii Rroxscaffold_5G00370040
rosa_rugosa Rorug04G0225200
rosa_samantha Rh4AG282200 Rh4BG287800 Rh4CG303500 Rh4DG284900
rosa_wichuraiana Rw4G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 116
AccB7I CCANNNNNTGG 3 cut(s) 52, 112, 481
AccII CGCG 4 cut(s) 75, 380, 453, 610
AciI CCGC 3 cut(s) 378, 509, 610
AclWI GGATC 2 cut(s) 25, 241
AcoI YGGCCR 1 cut(s) 283
AcuI CTGAAG 2 cut(s) 260, 282
AcyI GRCGYC 1 cut(s) 728
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 3 cut(s) 118, 293, 754
AfiI CCNNNNNNNGG 4 cut(s) 52, 112, 481, 555
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 1 cut(s) 355
AjnI CCWGG 2 cut(s) 105, 144
AluBI AGCT 7 cut(s) 82, 127, 184, 274, 280, 496, 598
AluI AGCT 7 cut(s) 82, 127, 184, 274, 280, 496, 598
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 2 cut(s) 181, 638
AlwI GGATC 2 cut(s) 25, 241
AoxI GGCC 2 cut(s) 142, 283
ApeKI GCWGC 3 cut(s) 79, 395, 595
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
Asp700I GAANNNNTTC 1 cut(s) 691
Asp718I GGTACC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 219
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 2 cut(s) 186, 636
BarI GAAGNNNNNNTAC 2 cut(s) 745, 777
BbsI GAAGAC 2 cut(s) 270, 663
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 3 cut(s) 91, 382, 607
BccI CCATC 7 cut(s) 46, 72, 128, 184, 439, 644, 796
BceAI ACGGC 3 cut(s) 157, 394, 585
BciT130I CCWGG 2 cut(s) 107, 146
BciVI GTATCC 1 cut(s) 441
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 181, 638
BfaI CTAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 106
BfuI GTATCC 1 cut(s) 441
BglII AGATCT 1 cut(s) 689
BisI GCNGC 5 cut(s) 80, 396, 510, 596, 611
BlsI GCNGC 5 cut(s) 81, 397, 511, 597, 612
BmcAI AGTACT 2 cut(s) 293, 754
Bme1390I CCNGG 3 cut(s) 107, 146, 251
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 3 cut(s) 107, 146, 251
BpiI GAAGAC 2 cut(s) 270, 663
BpmI CTGGAG 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 463, 650
BpuMI CCSGG 1 cut(s) 251
BsaBI GATNNNNATC 1 cut(s) 779
BsaHI GRCGYC 1 cut(s) 728
BsaJI CCNNGG 2 cut(s) 35, 623
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 112, 481, 555
Bse118I RCCGGY 1 cut(s) 216
Bse1I ACTGG 2 cut(s) 46, 319
Bse3DI GCAATG 2 cut(s) 342, 620
Bse8I GATNNNNATC 1 cut(s) 779
BseBI CCWGG 2 cut(s) 107, 146
BseDI CCNNGG 2 cut(s) 35, 623
BseGI GGATG 1 cut(s) 687
BseJI GATNNNNATC 1 cut(s) 779
BseLI CCNNNNNNNGG 4 cut(s) 52, 112, 481, 555
BseMI GCAATG 2 cut(s) 342, 620
BseNI ACTGG 2 cut(s) 46, 319
BseXI GCAGC 3 cut(s) 91, 382, 607
BsgI GTGCAG 1 cut(s) 98
Bsh1236I CGCG 4 cut(s) 75, 380, 453, 610
BshFI GGCC 2 cut(s) 144, 285
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 2 cut(s) 217, 251
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 4 cut(s) 52, 112, 481, 555
BsmAI GTCTC 2 cut(s) 181, 638
BsmBI CGTCTC 2 cut(s) 181, 638
BsmFI GGGAC 1 cut(s) 715
BsnI GGCC 2 cut(s) 144, 285
Bsp1286I GDGCHC 2 cut(s) 186, 636
Bsp143I GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
Bsp19I CCATGG 1 cut(s) 35
BspACI CCGC 3 cut(s) 378, 509, 610
BspANI GGCC 2 cut(s) 144, 285
BspFNI CGCG 4 cut(s) 75, 380, 453, 610
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 2 cut(s) 25, 241
BspQI GCTCTTC 1 cut(s) 650
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 2 cut(s) 342, 620
BsrFI RCCGGY 1 cut(s) 216
BsrI ACTGG 2 cut(s) 46, 319
BssAI RCCGGY 1 cut(s) 216
BssECI CCNNGG 2 cut(s) 35, 623
BssMI GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
BssNI GRCGYC 1 cut(s) 728
BssT1I CCWWGG 1 cut(s) 35
Bst2UI CCWGG 2 cut(s) 107, 146
Bst4CI ACNGT 1 cut(s) 576
Bst6I CTCTTC 1 cut(s) 650
BstACI GRCGYC 1 cut(s) 728
BstAPI GCANNNNNTGC 1 cut(s) 561
BstC8I GCNNGC 2 cut(s) 77, 407
BstDEI CTNAG 1 cut(s) 386
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 1 cut(s) 687
BstFNI CGCG 4 cut(s) 75, 380, 453, 610
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 7 cut(s) 33, 97, 156, 249, 692, 777, 783
BstMAI GTCTC 2 cut(s) 181, 638
BstMBI GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
BstMWI GCNNNNNNNGC 2 cut(s) 561, 619
BstNI CCWGG 2 cut(s) 107, 146
BstSCI CCNGG 3 cut(s) 105, 144, 249
BstUI CGCG 4 cut(s) 75, 380, 453, 610
BstV1I GCAGC 3 cut(s) 91, 382, 607
BstV2I GAAGAC 2 cut(s) 270, 663
BstX2I RGATCY 2 cut(s) 246, 689
BstYI RGATCY 2 cut(s) 246, 689
BsuI GTATCC 1 cut(s) 441
BsuRI GGCC 2 cut(s) 144, 285
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 1 cut(s) 687
Cac8I GCNNGC 2 cut(s) 77, 407
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 216
Cfr13I GGNCC 1 cut(s) 219
CpoI CGGWCCG 1 cut(s) 219
CseI GACGC 1 cut(s) 717
Csp6I GTAC 3 cut(s) 117, 292, 753
CspI CGGWCCG 1 cut(s) 219
CviAII CATG 2 cut(s) 36, 187
CviQI GTAC 3 cut(s) 117, 292, 753
DdeI CTNAG 1 cut(s) 386
DpnI GATC 7 cut(s) 32, 96, 155, 248, 691, 776, 782
DpnII GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
DraIII CACNNNGTG 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 35
Eco24I GRGCYC 2 cut(s) 186, 636
Eco47I GGWCC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 2 cut(s) 260, 282
EcoICRI GAGCTC 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 105, 144
EcoT14I CCWWGG 1 cut(s) 35
EcoT38I GRGCYC 2 cut(s) 186, 636
ErhI CCWWGG 1 cut(s) 35
Esp3I CGTCTC 2 cut(s) 181, 638
FaeI CATG 2 cut(s) 39, 190
FaiI YATR 4 cut(s) 37, 188, 234, 470
FalI AAGNNNNNCTT 2 cut(s) 655, 687
FaqI GGGAC 1 cut(s) 715
FatI CATG 2 cut(s) 35, 186
Fnu4HI GCNGC 5 cut(s) 80, 396, 510, 596, 611
FokI GGATG 1 cut(s) 694
FriOI GRGCYC 2 cut(s) 186, 636
Fsp4HI GCNGC 5 cut(s) 80, 396, 510, 596, 611
FspBI CTAG 1 cut(s) 750
GlaI GCGC 1 cut(s) 104
GluI GCNGC 5 cut(s) 80, 396, 510, 596, 611
GsuI CTGGAG 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 2 cut(s) 144, 285
HapII CCGG 2 cut(s) 217, 251
HgaI GACGC 1 cut(s) 717
HhaI GCGC 1 cut(s) 105
Hin1I GRCGYC 1 cut(s) 728
Hin1II CATG 2 cut(s) 39, 190
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 2 cut(s) 224, 365
HpaII CCGG 2 cut(s) 217, 251
Hpy188I TCNGA 9 cut(s) 139, 181, 201, 223, 239, 262, 423, 460, 641
Hpy188III TCNNGA 4 cut(s) 25, 187, 337, 778
Hpy99I CGWCG 3 cut(s) 143, 464, 571
HpyAV CCTTC 3 cut(s) 385, 482, 657
HpyCH4III ACNGT 1 cut(s) 576
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 3 cut(s) 79, 564, 580
HpyF10VI GCNNNNNNNGC 2 cut(s) 561, 619
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 728
Hsp92II CATG 2 cut(s) 39, 190
HspAI GCGC 1 cut(s) 103
KpnI GGTACC 1 cut(s) 120
Kzo9I GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
LguI GCTCTTC 1 cut(s) 650
Lsp1109I GCAGC 3 cut(s) 91, 382, 607
MaeI CTAG 1 cut(s) 750
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 86, 337
MalI GATC 7 cut(s) 32, 96, 155, 248, 691, 776, 782
MboI GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
MboII GAAGA 7 cut(s) 256, 275, 663, 667, 684, 699, 712
MflI RGATCY 2 cut(s) 246, 689
MhlI GDGCHC 2 cut(s) 186, 636
MluCI AATT 1 cut(s) 400
MluI ACGCGT 1 cut(s) 451
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 3 cut(s) 162, 462, 483
MnlI CCTC 7 cut(s) 315, 438, 478, 496, 520, 618, 642
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 1 cut(s) 722
MslI CAYNNNNRTG 1 cut(s) 436
MspI CCGG 2 cut(s) 217, 251
MspR9I CCNGG 3 cut(s) 107, 146, 251
MvaI CCWGG 2 cut(s) 107, 146
MvnI CGCG 4 cut(s) 75, 380, 453, 610
MwoI GCNNNNNNNGC 2 cut(s) 561, 619
NciI CCSGG 1 cut(s) 251
NcoI CCATGG 1 cut(s) 35
NdeII GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
NlaIII CATG 2 cut(s) 39, 190
NlaIV GGNNCC 1 cut(s) 118
NmeAIII GCCGAG 2 cut(s) 261, 648
NmuCI GTSAC 2 cut(s) 86, 337
PagI TCATGA 1 cut(s) 186
PciSI GCTCTTC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 1 cut(s) 224
PflMI CCANNNNNTGG 3 cut(s) 52, 112, 481
PkrI GCNGC 5 cut(s) 81, 397, 511, 597, 612
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 2 cut(s) 105, 144
PspGI CCWGG 2 cut(s) 105, 144
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 219
PsuI RGATCY 2 cut(s) 246, 689
RsaI GTAC 3 cut(s) 118, 293, 754
RsaNI GTAC 3 cut(s) 117, 292, 753
RseI CAYNNNNRTG 1 cut(s) 436
Rsr2I CGGWCCG 1 cut(s) 219
RsrII CGGWCCG 1 cut(s) 219
SacI GAGCTC 1 cut(s) 186
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 1 cut(s) 722
SatI GCNGC 5 cut(s) 80, 396, 510, 596, 611
Sau3AI GATC 7 cut(s) 30, 94, 153, 246, 689, 774, 780
Sau96I GGNCC 1 cut(s) 219
ScaI AGTACT 2 cut(s) 293, 754
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 3 cut(s) 107, 146, 251
SduI GDGCHC 2 cut(s) 186, 636
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 436
SmlI CTYRAG 2 cut(s) 442, 629
SmoI CTYRAG 2 cut(s) 442, 629
Sse9I AATT 1 cut(s) 400
SsiI CCGC 3 cut(s) 378, 509, 610
SspMI CTAG 1 cut(s) 750
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 3 cut(s) 105, 144, 249
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 576
TaiI ACGT 1 cut(s) 177
TasI AATT 1 cut(s) 400
TatI WGTACW 2 cut(s) 291, 752
TauI GCSGC 2 cut(s) 512, 613
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 722
Tru9I TTAA 1 cut(s) 722
TseFI GTSAC 2 cut(s) 86, 337
TseI GCWGC 3 cut(s) 79, 395, 595
Tsp45I GTSAC 2 cut(s) 86, 337
Van91I CCANNNNNTGG 3 cut(s) 52, 112, 481
VpaK11BI GGWCC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 1 cut(s) 750
ZrmI AGTACT 2 cut(s) 293, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.