FvH4_4g23500

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018421
Physical Location & Seq
Forward (+)
25957666 .. 25960870
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23500.t1

Sequence Viewer

Length: 1233 bp
ATGGCAACAGCAGCTCCGTTGGTGATCATAGCCAACCCGTTCAAATCGAACCAAGCCCACACAATCCGGTTGCACCCTCTTCGTCTTTCTTCAGGCCATAGCTTGATCAATCAAAGCAATCTTCGTTCTTGTTCTCCAATACGGAAGGACCTTAAACTTCAGTGCTTCTTCTCTAAGTTTAAGAAGAGGCCGGACTACTTAACCATCACCAAGTCAGAGCCAAAGTCAGGCGAGTCTCAGTTGCAGCCAAGAAATCAACTCCATGTTATCGTCAACAATTTGTGGGAAAGTTCTCTTAGGGCATTAAGGAATTCCGCAGTCGGTGCTGTGCTACCGGGTTTGTCAGTTATCATGTACAACCCTAAATCGAATCCGGCATTGGCTACCCCCGTCGGGCGGGTTGGGGGAAGCTTGTGTAAGACCAGGATCGACTCCGAGTATCGTCCGATCGGGCGGGTTAACGGAAGCTTTAATGGGAACTACGGCAACCTCAACTTAGGTTATTTTCAGAATTATACCCGCATTACTGAAATAGATGATCCGTATGTGGGACGAGCTGCGGCCTTTTATGTTTTCTGCTTCTTTGTTGGGGTTTTTTTTGTGGTGGTTAATTCTGAAGCTGGTGATGATTTGGTTACGAAGTTGAAATTGATCGGTTTGGCGTTTGTTCTTGGTATATTGTATTCGCGTCAAGTACAAACCAATGTAGTCAAGCTTCAGGTTCATTTGTCAGACACGGGGCGAGAACTCCAGACGGATCTTAATCGGATTGCTGAAAGTCAGGCTGCTGAAAGTGTGAACGGATACACTTATGCCTACAAGCACCACTGGATTATGGCTATTAGCCGACGGAACCGTTTCGTCGAGACAGTAGTAGCTATAGGTCGGCCTATGATTAATGGTTACTACATCTCAGGTTACTCATCAGTGGATTCACTGATGGGAGCTCGAGAGGCAAAAACTCACCTGAATAACCATTCTATTTACGAGAGGAATCATCCTAAAGAGACGAGCCATAGTTCAGCGAATGAATCGCAGAGTGAAGATATAGTGGTTACAATATTGGTGGCTGCTAAAGGTTCTTATCACATACCCGCCATCAAGGACAAGCATAGCTTAAAACAAGTGTTGGACATACTTCATTCCATTGGAAATTCAGACCGCATAAGAGCAGTGGAAGTGCTGTGGACTCCTCAGTTCTCTTCAAGTGCGGCAAGTATTTTACCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

411

Amino Acids

45.49

Weight (kDa)

9.61

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 234 - 400 5.5e-23 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018421)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23500
rosa_laevigata RLG00000007024
rosa_multiflora Rmu_sc0002225.1_g000013
rosa_roxburghii Rroxscaffold_5G00371730
rosa_rugosa Rorug02G0248500
rosa_samantha Rh4AG299900 Rh4BG307000 Rh4CG317300 Rh4DG298900
rosa_wichuraiana Rw4G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 688
AciI CCGC 8 cut(s) 315, 397, 454, 520, 560, 1095, 1162, 1211
AclWI GGATC 3 cut(s) 434, 533, 765
AcsI RAATTY 2 cut(s) 310, 1153
AcuI CTGAAG 4 cut(s) 75, 143, 636, 701
AfaI GTAC 2 cut(s) 356, 696
AfiI CCNNNNNNNGG 4 cut(s) 227, 393, 396, 548
AgsI TTSAA 3 cut(s) 43, 646, 1206
AjnI CCWGG 1 cut(s) 422
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
Alw21I GWGCWC 1 cut(s) 949
Alw26I GTCTC 3 cut(s) 240, 860, 1001
AlwI GGATC 3 cut(s) 434, 533, 765
Ama87I CYCGRG 1 cut(s) 948
AoxI GGCC 4 cut(s) 94, 188, 561, 887
ApeKI GCWGC 5 cut(s) 11, 244, 557, 785, 1070
ApoI RAATTY 2 cut(s) 310, 1153
AseI ATTAAT 1 cut(s) 897
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 1 cut(s) 148
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 4 cut(s) 34, 199, 635, 956
AvaI CYCGRG 1 cut(s) 948
AvaII GGWCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 949
BarI GAAGNNNNNNTAC 2 cut(s) 699, 731
Bbv12I GWGCWC 1 cut(s) 949
BbvI GCAGC 5 cut(s) 23, 256, 544, 772, 1057
BccI CCATC 3 cut(s) 212, 934, 1106
BceAI ACGGC 1 cut(s) 499
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 424
BciVI GTATCC 1 cut(s) 797
BclI TGATCA 2 cut(s) 24, 105
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 3 cut(s) 240, 860, 1001
BfmI CTRYAG 1 cut(s) 879
BfuI GTATCC 1 cut(s) 797
BisI GCNGC 7 cut(s) 12, 245, 558, 561, 786, 1071, 1212
BlsI GCNGC 7 cut(s) 13, 246, 559, 562, 787, 1072, 1213
Bme1390I CCNGG 2 cut(s) 336, 424
Bme18I GGWCC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 948
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 854
BmrFI CCNGG 2 cut(s) 336, 424
BpmI CTGGAG 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 336
BsaBI GATNNNNATC 1 cut(s) 762
BsaWI WCCGGW 1 cut(s) 66
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 393, 396, 548
Bse1I ACTGG 1 cut(s) 833
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 1 cut(s) 424
BseGI GGATG 1 cut(s) 997
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 4 cut(s) 227, 393, 396, 548
BseMII CTCAG 3 cut(s) 251, 927, 1208
BseNI ACTGG 1 cut(s) 833
BseRI GAGGAG 1 cut(s) 1182
BseXI GCAGC 5 cut(s) 23, 256, 544, 772, 1057
Bsh1236I CGCG 1 cut(s) 688
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 4 cut(s) 96, 190, 563, 889
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 949
BsiHKCI CYCGRG 1 cut(s) 948
BsiSI CCGG 4 cut(s) 67, 191, 335, 374
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 4 cut(s) 227, 393, 396, 548
BsmAI GTCTC 3 cut(s) 240, 860, 1001
BsmBI CGTCTC 1 cut(s) 1001
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 4 cut(s) 96, 190, 563, 889
BsoBI CYCGRG 1 cut(s) 948
Bsp1286I GDGCHC 1 cut(s) 949
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
BspACI CCGC 8 cut(s) 315, 397, 454, 520, 560, 1095, 1162, 1211
BspANI GGCC 4 cut(s) 96, 190, 563, 889
BspCNI CTCAG 3 cut(s) 250, 926, 1207
BspFNI CGCG 1 cut(s) 688
BspLI GGNNCC 1 cut(s) 854
BspPI GGATC 3 cut(s) 434, 533, 765
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 1 cut(s) 833
BssMI GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 2 cut(s) 857, 871
Bst6I CTCTTC 3 cut(s) 84, 179, 1207
BstAPI GCANNNNNTGC 1 cut(s) 323
BstAUI TGTACA 1 cut(s) 354
BstDEI CTNAG 6 cut(s) 174, 237, 296, 496, 913, 1194
BstF5I GGATG 1 cut(s) 997
BstFNI CGCG 1 cut(s) 688
BstKTI GATC 7 cut(s) 27, 108, 429, 450, 541, 654, 760
BstMAI GTCTC 3 cut(s) 240, 860, 1001
BstMBI GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 3 cut(s) 11, 323, 953
BstNI CCWGG 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 334, 422
BstSFI CTRYAG 1 cut(s) 879
BstUI CGCG 1 cut(s) 688
BstV1I GCAGC 5 cut(s) 23, 256, 544, 772, 1057
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 4 cut(s) 96, 190, 563, 889
BtsCI GGATG 1 cut(s) 997
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 5 cut(s) 167, 826, 933, 935, 1179
Cfr13I GGNCC 1 cut(s) 148
CseI GACGC 1 cut(s) 677
Csp6I GTAC 2 cut(s) 355, 695
CspCI CAANNNNNGTGG 2 cut(s) 1047, 1082
CviAII CATG 2 cut(s) 263, 352
CviQI GTAC 2 cut(s) 355, 695
DdeI CTNAG 6 cut(s) 174, 237, 296, 496, 913, 1194
DpnI GATC 7 cut(s) 26, 107, 428, 449, 540, 653, 759
DpnII GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
Eam1104I CTCTTC 3 cut(s) 84, 179, 1207
EarI CTCTTC 3 cut(s) 84, 179, 1207
Ecl136II GAGCTC 1 cut(s) 947
Eco24I GRGCYC 1 cut(s) 949
Eco47I GGWCC 1 cut(s) 148
Eco53kI GAGCTC 1 cut(s) 947
Eco57I CTGAAG 4 cut(s) 75, 143, 636, 701
Eco88I CYCGRG 1 cut(s) 948
EcoICRI GAGCTC 1 cut(s) 947
EcoO109I RGGNCCY 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 949
Esp3I CGTCTC 1 cut(s) 1001
FaeI CATG 2 cut(s) 266, 355
FalI AAGNNNNNCTT 2 cut(s) 1100, 1132
FaqI GGGAC 1 cut(s) 564
FatI CATG 2 cut(s) 262, 351
FauI CCCGC 4 cut(s) 390, 447, 527, 1102
FbaI TGATCA 2 cut(s) 24, 105
Fnu4HI GCNGC 7 cut(s) 12, 245, 558, 561, 786, 1071, 1212
FokI GGATG 1 cut(s) 984
FriOI GRGCYC 1 cut(s) 949
Fsp4HI GCNGC 7 cut(s) 12, 245, 558, 561, 786, 1071, 1212
GluI GCNGC 7 cut(s) 12, 245, 558, 561, 786, 1071, 1212
GsuI CTGGAG 1 cut(s) 734
HaeIII GGCC 4 cut(s) 96, 190, 563, 889
HapII CCGG 4 cut(s) 67, 191, 335, 374
HgaI GACGC 1 cut(s) 677
Hin1II CATG 2 cut(s) 266, 355
HincII GTYRAC 2 cut(s) 274, 460
HindII GTYRAC 2 cut(s) 274, 460
HindIII AAGCTT 3 cut(s) 409, 466, 713
HinfI GANTC 7 cut(s) 233, 370, 431, 932, 994, 1031, 1189
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 4 cut(s) 67, 191, 335, 374
HphI GGTGA 4 cut(s) 34, 199, 635, 956
Hpy166II GTNNAC 4 cut(s) 274, 460, 799, 1188
Hpy188I TCNGA 8 cut(s) 217, 436, 447, 510, 616, 733, 768, 1159
Hpy188III TCNNGA 3 cut(s) 751, 865, 950
Hpy8I GTNNAC 4 cut(s) 274, 460, 799, 1188
Hpy99I CGWCG 3 cut(s) 395, 852, 866
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 2 cut(s) 857, 871
HpyCH4V TGCA 2 cut(s) 73, 244
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 323, 953
HpyF3I CTNAG 6 cut(s) 174, 237, 296, 496, 913, 1194
Hsp92II CATG 2 cut(s) 266, 355
Ksp22I TGATCA 2 cut(s) 24, 105
KspAI GTTAAC 1 cut(s) 460
Kzo9I GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
LmnI GCTCC 2 cut(s) 19, 944
Lsp1109I GCAGC 5 cut(s) 23, 256, 544, 772, 1057
MaeIII GTNAC 4 cut(s) 634, 902, 917, 1054
MalI GATC 7 cut(s) 26, 107, 428, 449, 540, 653, 759
MboI GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
MboII GAAGA 7 cut(s) 71, 81, 113, 160, 196, 1055, 1194
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 1 cut(s) 949
MluCI AATT 6 cut(s) 277, 310, 511, 610, 647, 1153
MlyI GAGTC 3 cut(s) 242, 425, 1183
MmeI TCCRAC 1 cut(s) 1110
MnlI CCTC 6 cut(s) 87, 180, 500, 946, 984, 1203
MroXI GAANNNNTTC 1 cut(s) 857
MspI CCGG 4 cut(s) 67, 191, 335, 374
MspR9I CCNGG 2 cut(s) 336, 424
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 688
MwoI GCNNNNNNNGC 3 cut(s) 11, 323, 953
NciI CCSGG 1 cut(s) 336
NdeII GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
NlaIII CATG 2 cut(s) 266, 355
NlaIV GGNNCC 1 cut(s) 854
PaeR7I CTCGAG 1 cut(s) 948
PdmI GAANNNNTTC 1 cut(s) 857
PfeI GAWTC 4 cut(s) 370, 932, 994, 1031
PkrI GCNGC 7 cut(s) 13, 246, 559, 562, 787, 1072, 1213
Ple19I CGATCG 1 cut(s) 450
PleI GAGTC 3 cut(s) 241, 425, 1183
PpsI GAGTC 3 cut(s) 241, 425, 1183
PpuMI RGGWCCY 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 897
Psp124BI GAGCTC 1 cut(s) 949
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 1 cut(s) 854
PspPI GGNCC 1 cut(s) 148
PspPPI RGGWCCY 1 cut(s) 148
PsuI RGATCY 1 cut(s) 757
PvuI CGATCG 1 cut(s) 450
RsaI GTAC 2 cut(s) 356, 696
RsaNI GTAC 2 cut(s) 355, 695
SacI GAGCTC 1 cut(s) 949
SatI GCNGC 7 cut(s) 12, 245, 558, 561, 786, 1071, 1212
Sau3AI GATC 7 cut(s) 24, 105, 426, 447, 538, 651, 757
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 3 cut(s) 242, 425, 1183
ScrFI CCNGG 2 cut(s) 336, 424
SduI GDGCHC 1 cut(s) 949
SfcI CTRYAG 1 cut(s) 879
Sfr274I CTCGAG 1 cut(s) 948
SinI GGWCC 1 cut(s) 148
SlaI CTCGAG 1 cut(s) 948
SmlI CTYRAG 1 cut(s) 948
SmoI CTYRAG 1 cut(s) 948
Sse9I AATT 6 cut(s) 277, 310, 511, 610, 647, 1153
SsiI CCGC 8 cut(s) 315, 397, 454, 520, 560, 1095, 1162, 1211
SspI AATATT 1 cut(s) 1062
SstI GAGCTC 1 cut(s) 949
StyD4I CCNGG 2 cut(s) 334, 422
TaaI ACNGT 2 cut(s) 857, 871
TaqI TCGA 5 cut(s) 47, 368, 429, 864, 949
TasI AATT 6 cut(s) 277, 310, 511, 610, 647, 1153
TatI WGTACW 2 cut(s) 354, 694
TauI GCSGC 2 cut(s) 563, 1214
TfiI GAWTC 4 cut(s) 370, 932, 994, 1031
TscAI CASTG 5 cut(s) 167, 833, 933, 942, 1179
TseI GCWGC 5 cut(s) 11, 244, 557, 785, 1070
TspDTI ATGAA 3 cut(s) 713, 1044, 1130
TspGWI ACGGA 7 cut(s) 6, 157, 477, 531, 770, 816, 865
TspRI CASTG 5 cut(s) 167, 833, 933, 942, 1179
VpaK11BI GGWCC 1 cut(s) 148
VspI ATTAAT 1 cut(s) 897
XapI RAATTY 2 cut(s) 310, 1153
XhoI CTCGAG 1 cut(s) 948
XmnI GAANNNNTTC 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.