Rw4G025590

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018421
Physical Location & Seq
Forward (+)
51705111 .. 51707061
1951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025590.1

Sequence Viewer

Length: 843 bp
ATGGGTGCCGTGCTGCTGGCTTTATTAGTCATGTACAACCCGAAATCGAATTCGGCATTGGCTGCCCAGATCGGGCGGGTTGGGGGAAGCTGGTCTACCTGTTCCCCCCACTACATTATTGACCATGATGATCCGTATCCGGAGGATGTTACAGCTTGTTATGTTTTCTGCTTCATTCTTGCGGGTTTTTTTGTGGTGGTTACTTCTGCTTTTGGTGATGTTTCGTGGATGGAATTGAAATTGATCGGTGTGGCAGCTGTTCTGGCTATTTTGGCATGGAAAGATGTACGAACCAATGTAGTCAAGCTTCAGGTTCGTTTGTCAGACACGGGGCAAGCACTCCAAACGGATCTTAATCGGATTGCTGAAAGTGTAGATGGAGACACTTCTAGCTATAGGAGTTTGAGCTATATCTTGAATGAGACAGTTATAGCTTTGTTTCGGCGTCATGGTTACTACATCTCAGGTTATTCATCAGTGGCTTCACTGGCCGGAGGTCGAGCTGCAAAGATTTACCTGAATTACCTTTCTATTAACGAGAGGATTCATCATAAAGAGACGCTTGTTAATGTAAACAACATTAAAGCCCAAAGCTTGCCGAGCCATAGTTCAGCTAATGAATCGCAGAGTGAACATATAGTGGTTACAATATTGGTGGCTGCTAAAGGTCGGGATCACATACCCGCCATCAATGACAGTGATGGCTTGGAAAAAGTGTTGCACATACTTCTTTCCATTGGTCATTCAGACCGCATAAGAGCAGTGGAAGTGTTGTGGACTCATCACTTCTCTACGAGTGCGGCAAGTATCTGTAAATTACCTGCTTCCGAGGTGCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

280

Amino Acids

30.52

Weight (kDa)

6.44

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 98 - 260 1.2e-33 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018421)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23500
rosa_laevigata RLG00000007024
rosa_multiflora Rmu_sc0002225.1_g000013
rosa_roxburghii Rroxscaffold_5G00371730
rosa_rugosa Rorug02G0248500
rosa_samantha Rh4AG299900 Rh4BG307000 Rh4CG317300 Rh4DG298900
rosa_wichuraiana Rw4G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 829
AccB1I GGYRCC 2 cut(s) 5, 832
AccI GTMKAC 1 cut(s) 95
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 5 cut(s) 76, 182, 684, 751, 800
AclWI GGATC 3 cut(s) 125, 357, 681
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 293
AcyI GRCGYC 1 cut(s) 445
AfaI GTAC 2 cut(s) 35, 288
AfiI CCNNNNNNNGG 1 cut(s) 72
AgsI TTSAA 2 cut(s) 238, 418
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
Alw26I GTCTC 3 cut(s) 375, 416, 551
AlwI GGATC 3 cut(s) 125, 357, 681
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 5 cut(s) 13, 62, 254, 503, 659
ApoI RAATTY 1 cut(s) 49
AsuHPI GGTGA 1 cut(s) 227
BanI GGYRCC 2 cut(s) 5, 832
BarI GAAGNNNNNNTAC 4 cut(s) 79, 111, 291, 323
BbvI GCAGC 4 cut(s) 49, 266, 490, 646
BccI CCATC 4 cut(s) 223, 371, 695, 695
BciVI GTATCC 1 cut(s) 147
BcoDI GTCTC 3 cut(s) 375, 416, 551
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 829
BfuI GTATCC 1 cut(s) 147
BisI GCNGC 6 cut(s) 14, 63, 255, 504, 660, 801
BlsI GCNGC 6 cut(s) 15, 64, 256, 505, 661, 802
BmiI GGNNCC 2 cut(s) 7, 834
BsaBI GATNNNNATC 2 cut(s) 135, 354
BsaHI GRCGYC 1 cut(s) 445
BsaJI CCNNGG 1 cut(s) 828
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 492
Bse8I GATNNNNATC 2 cut(s) 135, 354
BseAI TCCGGA 1 cut(s) 139
BseDI CCNNGG 1 cut(s) 828
BseGI GGATG 2 cut(s) 151, 234
BseJI GATNNNNATC 2 cut(s) 135, 354
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 492
BseXI GCAGC 4 cut(s) 49, 266, 490, 646
BshFI GGCC 1 cut(s) 491
BshNI GGYRCC 2 cut(s) 5, 832
BsiSI CCGG 2 cut(s) 140, 492
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 3 cut(s) 375, 416, 551
BsmBI CGTCTC 1 cut(s) 551
BsnI GGCC 1 cut(s) 491
Bsp13I TCCGGA 1 cut(s) 139
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 5 cut(s) 69, 130, 243, 349, 673
BspACI CCGC 5 cut(s) 76, 182, 684, 751, 800
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 1 cut(s) 476
BspEI TCCGGA 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 7, 834
BspMI ACCTGC 1 cut(s) 829
BspPI GGATC 3 cut(s) 125, 357, 681
BspT107I GGYRCC 2 cut(s) 5, 832
BsrGI TGTACA 1 cut(s) 33
BsrI ACTGG 1 cut(s) 492
BssECI CCNNGG 1 cut(s) 828
BssMI GATC 5 cut(s) 69, 130, 243, 349, 673
BssNI GRCGYC 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 427, 698
BstACI GRCGYC 1 cut(s) 445
BstAPI GCANNNNNTGC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 18, 336, 596
BstDEI CTNAG 1 cut(s) 463
BstF5I GGATG 2 cut(s) 151, 234
BstKTI GATC 5 cut(s) 72, 133, 246, 352, 676
BstMAI GTCTC 3 cut(s) 375, 416, 551
BstMBI GATC 5 cut(s) 69, 130, 243, 349, 673
BstMWI GCNNNNNNNGC 5 cut(s) 62, 263, 272, 488, 600
BstSFI CTRYAG 1 cut(s) 394
BstV1I GCAGC 4 cut(s) 49, 266, 490, 646
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuI GTATCC 1 cut(s) 147
BsuRI GGCC 1 cut(s) 491
BtsCI GGATG 2 cut(s) 151, 234
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 4 cut(s) 483, 485, 703, 768
BveI ACCTGC 1 cut(s) 829
Cac8I GCNNGC 3 cut(s) 18, 336, 596
CseI GACGC 2 cut(s) 434, 568
Csp6I GTAC 2 cut(s) 34, 287
CspCI CAANNNNNGTGG 2 cut(s) 636, 671
CviAII CATG 4 cut(s) 31, 125, 276, 449
CviQI GTAC 2 cut(s) 34, 287
DdeI CTNAG 1 cut(s) 463
DpnI GATC 5 cut(s) 71, 132, 245, 351, 675
DpnII GATC 5 cut(s) 69, 130, 243, 349, 673
EaeI YGGCCR 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 49
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 4 cut(s) 34, 128, 279, 452
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FatI CATG 4 cut(s) 30, 124, 275, 448
FauI CCCGC 3 cut(s) 69, 175, 691
FblI GTMKAC 1 cut(s) 95
Fnu4HI GCNGC 6 cut(s) 14, 63, 255, 504, 660, 801
FokI GGATG 2 cut(s) 158, 241
Fsp4HI GCNGC 6 cut(s) 14, 63, 255, 504, 660, 801
FspBI CTAG 1 cut(s) 390
GluI GCNGC 6 cut(s) 14, 63, 255, 504, 660, 801
HaeIII GGCC 1 cut(s) 491
HapII CCGG 2 cut(s) 140, 492
HgaI GACGC 2 cut(s) 434, 568
Hin1I GRCGYC 1 cut(s) 445
Hin1II CATG 4 cut(s) 34, 128, 279, 452
HindIII AAGCTT 2 cut(s) 305, 592
HinfI GANTC 3 cut(s) 544, 620, 778
HpaII CCGG 2 cut(s) 140, 492
HphI GGTGA 1 cut(s) 227
Hpy166II GTNNAC 4 cut(s) 96, 574, 632, 777
Hpy188I TCNGA 4 cut(s) 325, 360, 748, 829
Hpy188III TCNNGA 3 cut(s) 140, 415, 671
Hpy8I GTNNAC 4 cut(s) 96, 574, 632, 777
HpyCH4III ACNGT 2 cut(s) 427, 698
HpyCH4V TGCA 2 cut(s) 506, 721
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 263, 272, 488, 600
HpyF3I CTNAG 1 cut(s) 463
Hsp92I GRCGYC 1 cut(s) 445
Hsp92II CATG 4 cut(s) 34, 128, 279, 452
Kpn2I TCCGGA 1 cut(s) 139
Kzo9I GATC 5 cut(s) 69, 130, 243, 349, 673
Lsp1109I GCAGC 4 cut(s) 49, 266, 490, 646
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 4 cut(s) 148, 199, 452, 643
MalI GATC 5 cut(s) 71, 132, 245, 351, 675
MboI GATC 5 cut(s) 69, 130, 243, 349, 673
MflI RGATCY 1 cut(s) 349
MluCI AATT 5 cut(s) 49, 233, 239, 520, 815
MlyI GAGTC 1 cut(s) 772
MnlI CCTC 4 cut(s) 136, 488, 534, 823
MroI TCCGGA 1 cut(s) 139
MseI TTAA 4 cut(s) 354, 534, 567, 582
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 2 cut(s) 140, 492
MwoI GCNNNNNNNGC 5 cut(s) 62, 263, 272, 488, 600
NdeII GATC 5 cut(s) 69, 130, 243, 349, 673
NlaIII CATG 4 cut(s) 34, 128, 279, 452
NlaIV GGNNCC 2 cut(s) 7, 834
NmeAIII GCCGAG 1 cut(s) 624
PfeI GAWTC 2 cut(s) 544, 620
PkrI GCNGC 6 cut(s) 15, 64, 256, 505, 661, 802
PleI GAGTC 1 cut(s) 772
PpsI GAGTC 1 cut(s) 772
PspN4I GGNNCC 2 cut(s) 7, 834
PsuI RGATCY 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 35, 288
RsaNI GTAC 2 cut(s) 34, 287
SaqAI TTAA 4 cut(s) 354, 534, 567, 582
SatI GCNGC 6 cut(s) 14, 63, 255, 504, 660, 801
Sau3AI GATC 5 cut(s) 69, 130, 243, 349, 673
SchI GAGTC 1 cut(s) 772
SfcI CTRYAG 1 cut(s) 394
Sse9I AATT 5 cut(s) 49, 233, 239, 520, 815
SsiI CCGC 5 cut(s) 76, 182, 684, 751, 800
SspI AATATT 1 cut(s) 651
SspMI CTAG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 427, 698
TaqI TCGA 2 cut(s) 47, 499
TasI AATT 5 cut(s) 49, 233, 239, 520, 815
TatI WGTACW 1 cut(s) 33
TauI GCSGC 1 cut(s) 803
TfiI GAWTC 2 cut(s) 544, 620
Tru1I TTAA 4 cut(s) 354, 534, 567, 582
Tru9I TTAA 4 cut(s) 354, 534, 567, 582
TscAI CASTG 4 cut(s) 483, 492, 703, 768
TseI GCWGC 5 cut(s) 13, 62, 254, 503, 659
TspDTI ATGAA 4 cut(s) 163, 462, 536, 633
TspGWI ACGGA 2 cut(s) 123, 362
TspRI CASTG 4 cut(s) 483, 492, 703, 768
XapI RAATTY 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 95
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.