Rh4BG307000

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0018421
Physical Location & Seq
Reverse (-)
47642165 .. 47661912
19748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG307000.1

Sequence Viewer

Length: 1047 bp
ATGGAAAAGGTCCTGACTGAATCTGAGAAGAAGCCGGCGGACTACTTAACCACCACCGAGTCAAAGTCAAAATCAGGAGAGTCTCAGTGGCGTCCAAGAAACCAAATCCACATTAGCGCCAATAATATGTGGGAAAGTTCTCTTAGGGCATTAAGGAAATCCGCAATCGGTGCCGTACTGCTGGGTTTATTAGTCGTGTACAACCCGAAATCGAATTCGGCATTGGCTGCCCCGGTCGGGCGGGTTGGGGGAAGCTGGTCTACCTGGTCACCATCGTACTCTATATATGTATATAGCCACTACAATATTAACCATGATAACCATGATGATCCGTATCCGGCTGGAGTTGCAGCTTGTTATTTTTTCTACTTCATTGTTGCGGCTTTTTTTGTGGTGGCTAGTTCTGGTTTTGATGATGTTTCAGTTTCGTGGTTGAAATTGAAATTGATCGGTTTGGCAGTTGTTCTAGCTACTTTGGCTTGGTGTGATGTACAAACCAATGTAGTCAAGCTTCAGGTTTGTTTGTCAGACACGGGCCGAGCACTCCAGACGGATCTTAATCGGATTGCTGAAAGTGTACATGGAGACACTTCTAGCTATAAGAGTTTGAGCTATGTCTTGAATGAGACAGTAATAGCTTTGTTTCGGCGCCATGGTTACTACATCTCAGGTTATTCATCAGTGGCTTCACAGACCGGAGGTCGAGCTGCAAAGATTTACCTGAATTACCTTTCTATTAACGAGAGGATTCATCAAAAAGAGACGCTTGTTAATGTAAACAACATGAAAGCAGGAAGCTCGCCGAGCCATAGTCCAGCTAATGAATCGCAGAATGAACATGTAGTGGTTACAATATTGGTAGCTGCTAAAGGTTGGGATCACATACCCGCCATCTATGACAGTCATAGCTTGGAAAAAGTGTTGCACATACTTCTTTCCATTGGTCATTCAAACCGCATAAGAGCAGTGGAAGTGTTGTGGACTCCTCAGTTCTTTTCGAGTGCGGCAAGTATCTGTAAATTACCCGCTTCCGAGGTGCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

348

Amino Acids

38.31

Weight (kDa)

8.52

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 166 - 330 1.4e-34 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018421)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23500
rosa_laevigata RLG00000007024
rosa_multiflora Rmu_sc0002225.1_g000013
rosa_roxburghii Rroxscaffold_5G00371730
rosa_rugosa Rorug02G0248500
rosa_samantha Rh4AG299900 Rh4BG307000 Rh4CG317300 Rh4DG298900
rosa_wichuraiana Rw4G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 170, 648, 1036
AccI GTMKAC 1 cut(s) 260
AciI CCGC 8 cut(s) 38, 162, 241, 380, 888, 955, 1004, 1026
AclWI GGATC 3 cut(s) 323, 561, 885
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 497
AcyI GRCGYC 2 cut(s) 91, 649
AfaI GTAC 5 cut(s) 177, 200, 278, 492, 579
AfiI CCNNNNNNNGG 1 cut(s) 237
AflIII ACRYGT 1 cut(s) 838
AgsI TTSAA 4 cut(s) 436, 442, 622, 951
AhdI GACNNNNNGTC 1 cut(s) 699
AjnI CCWGG 1 cut(s) 263
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 4 cut(s) 87, 579, 620, 755
AlwI GGATC 3 cut(s) 323, 561, 885
AoxI GGCC 1 cut(s) 535
ApeKI GCWGC 4 cut(s) 227, 350, 707, 863
ApoI RAATTY 1 cut(s) 214
AspLEI GCGC 2 cut(s) 119, 651
AspS9I GGNCC 2 cut(s) 10, 535
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 261
AvaII GGWCC 1 cut(s) 10
BanI GGYRCC 3 cut(s) 170, 648, 1036
BarI GAAGNNNNNNTAC 4 cut(s) 244, 276, 495, 527
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 4 cut(s) 214, 362, 694, 850
BccI CCATC 2 cut(s) 280, 899
BceAI ACGGC 1 cut(s) 158
BcgI CGANNNNNNTGC 2 cut(s) 780, 814
BciT130I CCWGG 1 cut(s) 265
BciVI GTATCC 1 cut(s) 345
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 4 cut(s) 87, 579, 620, 755
BfaI CTAG 3 cut(s) 399, 467, 594
BfoI RGCGCY 2 cut(s) 120, 652
BfuI GTATCC 1 cut(s) 345
BisI GCNGC 6 cut(s) 228, 351, 381, 708, 864, 1005
BlsI GCNGC 6 cut(s) 229, 352, 382, 709, 865, 1006
Bme1390I CCNGG 2 cut(s) 233, 265
Bme18I GGWCC 1 cut(s) 10
BmeRI GACNNNNNGTC 1 cut(s) 699
BmgT120I GGNCC 2 cut(s) 10, 535
BmiI GGNNCC 3 cut(s) 172, 650, 1038
BmrFI CCNGG 2 cut(s) 233, 265
BpmI CTGGAG 2 cut(s) 363, 530
BpuMI CCSGG 1 cut(s) 233
BsaBI GATNNNNATC 2 cut(s) 333, 558
BsaHI GRCGYC 2 cut(s) 91, 649
BsaJI CCNNGG 3 cut(s) 231, 652, 1032
BsaWI WCCGGW 1 cut(s) 695
Bsc4I CCNNNNNNNGG 1 cut(s) 237
Bse118I RCCGGY 1 cut(s) 34
Bse8I GATNNNNATC 2 cut(s) 333, 558
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 3 cut(s) 231, 652, 1032
BseJI GATNNNNATC 2 cut(s) 333, 558
BseLI CCNNNNNNNGG 1 cut(s) 237
BseMII CTCAG 4 cut(s) 15, 98, 681, 1001
BseRI GAGGAG 1 cut(s) 975
BseXI GCAGC 4 cut(s) 214, 362, 694, 850
BseYI CCCAGC 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 1 cut(s) 537
BshNI GGYRCC 3 cut(s) 170, 648, 1036
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 544
BsiSI CCGG 4 cut(s) 35, 233, 338, 696
BslI CCNNNNNNNGG 1 cut(s) 237
BsmAI GTCTC 4 cut(s) 87, 579, 620, 755
BsmBI CGTCTC 1 cut(s) 755
BsnI GGCC 1 cut(s) 537
Bsp1286I GDGCHC 1 cut(s) 544
Bsp1407I TGTACA 3 cut(s) 198, 490, 577
Bsp143I GATC 4 cut(s) 328, 447, 553, 877
Bsp19I CCATGG 1 cut(s) 652
BspACI CCGC 8 cut(s) 38, 162, 241, 380, 888, 955, 1004, 1026
BspANI GGCC 1 cut(s) 537
BspCNI CTCAG 4 cut(s) 16, 97, 680, 1000
BspLI GGNNCC 3 cut(s) 172, 650, 1038
BspPI GGATC 3 cut(s) 323, 561, 885
BspT107I GGYRCC 3 cut(s) 170, 648, 1036
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 3 cut(s) 198, 490, 577
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 231, 652, 1032
BssMI GATC 4 cut(s) 328, 447, 553, 877
BssNI GRCGYC 2 cut(s) 91, 649
BssT1I CCWWGG 1 cut(s) 652
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 2 cut(s) 631, 902
BstACI GRCGYC 2 cut(s) 91, 649
BstAPI GCANNNNNTGC 2 cut(s) 170, 227
BstAUI TGTACA 3 cut(s) 198, 490, 577
BstC8I GCNNGC 2 cut(s) 36, 800
BstDEI CTNAG 5 cut(s) 24, 84, 143, 667, 987
BstDSI CCRYGG 1 cut(s) 652
BstEII GGTNACC 1 cut(s) 267
BstH2I RGCGCY 2 cut(s) 120, 652
BstHHI GCGC 2 cut(s) 119, 651
BstKTI GATC 4 cut(s) 331, 450, 556, 880
BstMAI GTCTC 4 cut(s) 87, 579, 620, 755
BstMBI GATC 4 cut(s) 328, 447, 553, 877
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 5 cut(s) 170, 227, 347, 476, 804
BstNI CCWGG 1 cut(s) 265
BstNSI RCATGY 1 cut(s) 842
BstPI GGTNACC 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 231, 263
BstV1I GCAGC 4 cut(s) 214, 362, 694, 850
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 1 cut(s) 537
BtgI CCRYGG 1 cut(s) 652
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 3 cut(s) 92, 687, 972
Cac8I GCNNGC 2 cut(s) 36, 800
CfoI GCGC 2 cut(s) 119, 651
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 10, 535
CseI GACGC 2 cut(s) 80, 772
CsiI ACCWGGT 1 cut(s) 263
Csp6I GTAC 5 cut(s) 176, 199, 277, 491, 578
CviAII CATG 6 cut(s) 314, 323, 581, 653, 784, 839
CviQI GTAC 5 cut(s) 176, 199, 277, 491, 578
DdeI CTNAG 5 cut(s) 24, 84, 143, 667, 987
DinI GGCGCC 1 cut(s) 650
DpnI GATC 4 cut(s) 330, 449, 555, 879
DpnII GATC 4 cut(s) 328, 447, 553, 877
DriI GACNNNNNGTC 1 cut(s) 699
Eam1105I GACNNNNNGTC 1 cut(s) 699
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 652
EgeI GGCGCC 1 cut(s) 650
EheI GGCGCC 1 cut(s) 650
ErhI CCWWGG 1 cut(s) 652
Esp3I CGTCTC 1 cut(s) 755
FaeI CATG 6 cut(s) 317, 326, 584, 656, 787, 842
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FatI CATG 6 cut(s) 313, 322, 580, 652, 783, 838
FauI CCCGC 3 cut(s) 234, 895, 1033
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 6 cut(s) 228, 351, 381, 708, 864, 1005
Fsp4HI GCNGC 6 cut(s) 228, 351, 381, 708, 864, 1005
FspBI CTAG 3 cut(s) 399, 467, 594
GlaI GCGC 2 cut(s) 118, 650
GluI GCNGC 6 cut(s) 228, 351, 381, 708, 864, 1005
GsaI CCCAGC 1 cut(s) 185
GsuI CTGGAG 2 cut(s) 363, 530
HaeII RGCGCY 2 cut(s) 120, 652
HaeIII GGCC 1 cut(s) 537
HapII CCGG 4 cut(s) 35, 233, 338, 696
HgaI GACGC 2 cut(s) 80, 772
HhaI GCGC 2 cut(s) 119, 651
Hin1I GRCGYC 2 cut(s) 91, 649
Hin1II CATG 6 cut(s) 317, 326, 584, 656, 787, 842
Hin6I GCGC 2 cut(s) 117, 649
HinP1I GCGC 2 cut(s) 117, 649
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 6 cut(s) 20, 59, 80, 748, 824, 982
HpaII CCGG 4 cut(s) 35, 233, 338, 696
HphI GGTGA 1 cut(s) 261
Hpy166II GTNNAC 5 cut(s) 199, 261, 578, 778, 981
Hpy188I TCNGA 4 cut(s) 25, 529, 564, 1033
Hpy188III TCNNGA 4 cut(s) 13, 75, 547, 619
Hpy8I GTNNAC 5 cut(s) 199, 261, 578, 778, 981
HpyCH4III ACNGT 2 cut(s) 631, 902
HpyCH4V TGCA 3 cut(s) 350, 710, 925
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 227, 347, 476, 804
HpyF3I CTNAG 5 cut(s) 24, 84, 143, 667, 987
Hsp92I GRCGYC 2 cut(s) 91, 649
Hsp92II CATG 6 cut(s) 317, 326, 584, 656, 787, 842
HspAI GCGC 2 cut(s) 117, 649
KasI GGCGCC 1 cut(s) 648
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Kzo9I GATC 4 cut(s) 328, 447, 553, 877
Lsp1109I GCAGC 4 cut(s) 214, 362, 694, 850
MabI ACCWGGT 1 cut(s) 263
MaeI CTAG 3 cut(s) 399, 467, 594
MaeIII GTNAC 3 cut(s) 267, 656, 847
MalI GATC 4 cut(s) 330, 449, 555, 879
MboI GATC 4 cut(s) 328, 447, 553, 877
MboII GAAGA 1 cut(s) 40
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 5 cut(s) 214, 437, 443, 724, 1019
Mly113I GGCGCC 1 cut(s) 649
MlyI GAGTC 3 cut(s) 68, 89, 976
MnlI CCTC 4 cut(s) 692, 738, 996, 1027
MroNI GCCGGC 1 cut(s) 34
MseI TTAA 6 cut(s) 47, 152, 309, 558, 738, 771
MspI CCGG 4 cut(s) 35, 233, 338, 696
MspR9I CCNGG 2 cut(s) 233, 265
MvaI CCWGG 1 cut(s) 265
MwoI GCNNNNNNNGC 5 cut(s) 170, 227, 347, 476, 804
NaeI GCCGGC 1 cut(s) 36
NarI GGCGCC 1 cut(s) 649
NciI CCSGG 1 cut(s) 233
NcoI CCATGG 1 cut(s) 652
NdeII GATC 4 cut(s) 328, 447, 553, 877
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 6 cut(s) 317, 326, 584, 656, 787, 842
NlaIV GGNNCC 3 cut(s) 172, 650, 1038
NmeAIII GCCGAG 2 cut(s) 563, 828
NmuCI GTSAC 1 cut(s) 267
NspI RCATGY 1 cut(s) 842
PciI ACATGT 1 cut(s) 838
PdiI GCCGGC 1 cut(s) 36
PfeI GAWTC 3 cut(s) 20, 748, 824
PkrI GCNGC 6 cut(s) 229, 352, 382, 709, 865, 1006
PleI GAGTC 3 cut(s) 67, 88, 976
PluTI GGCGCC 1 cut(s) 652
PpsI GAGTC 3 cut(s) 67, 88, 976
PpuMI RGGWCCY 1 cut(s) 10
PscI ACATGT 1 cut(s) 838
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 263
PspEI GGTNACC 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 181
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 172, 650, 1038
PspPI GGNCC 2 cut(s) 10, 535
PspPPI RGGWCCY 1 cut(s) 10
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 5 cut(s) 177, 200, 278, 492, 579
RsaNI GTAC 5 cut(s) 176, 199, 277, 491, 578
SaqAI TTAA 6 cut(s) 47, 152, 309, 558, 738, 771
SatI GCNGC 6 cut(s) 228, 351, 381, 708, 864, 1005
Sau3AI GATC 4 cut(s) 328, 447, 553, 877
Sau96I GGNCC 2 cut(s) 10, 535
SchI GAGTC 3 cut(s) 68, 89, 976
ScrFI CCNGG 2 cut(s) 233, 265
SduI GDGCHC 1 cut(s) 544
SexAI ACCWGGT 1 cut(s) 263
SfoI GGCGCC 1 cut(s) 650
SinI GGWCC 1 cut(s) 10
Sse9I AATT 5 cut(s) 214, 437, 443, 724, 1019
SsiI CCGC 8 cut(s) 38, 162, 241, 380, 888, 955, 1004, 1026
SspDI GGCGCC 1 cut(s) 648
SspI AATATT 2 cut(s) 307, 855
SspMI CTAG 3 cut(s) 399, 467, 594
StyD4I CCNGG 2 cut(s) 231, 263
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 2 cut(s) 631, 902
TaqI TCGA 3 cut(s) 212, 703, 998
TasI AATT 5 cut(s) 214, 437, 443, 724, 1019
TatI WGTACW 3 cut(s) 198, 490, 577
TauI GCSGC 2 cut(s) 383, 1007
TfiI GAWTC 3 cut(s) 20, 748, 824
Tru1I TTAA 6 cut(s) 47, 152, 309, 558, 738, 771
Tru9I TTAA 6 cut(s) 47, 152, 309, 558, 738, 771
TscAI CASTG 3 cut(s) 92, 687, 972
TseFI GTSAC 1 cut(s) 267
TseI GCWGC 4 cut(s) 227, 350, 707, 863
Tsp45I GTSAC 1 cut(s) 267
TspDTI ATGAA 6 cut(s) 361, 666, 740, 800, 837, 849
TspGWI ACGGA 2 cut(s) 321, 566
TspRI CASTG 3 cut(s) 92, 687, 972
VpaK11BI GGWCC 1 cut(s) 10
XapI RAATTY 1 cut(s) 214
XceI RCATGY 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 260
XspI CTAG 3 cut(s) 399, 467, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.