Rroxscaffold_5G00371730

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0018421
Physical Location & Seq
Forward (+)
53279740 .. 53282138
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371730.1

Sequence Viewer

Length: 1101 bp
ATGGCAACAGCAGCTGCGTTGGTGATCCAAGCCAACTCATTCAAATTCAAATGGAACGAAGCCCACACAATCCGGTTGCACCCTCTTCGTCGTTCCAAAGACATTAGCTTGAATTCCAACAATCTTCGTTCTTGTTCTCCAATACGGAAGGACCTTAAACTTAAATGCTTCTTTTTAAACTCAGAGAAGAAGCCGGCGGACTACTTTACCACCACCGAGTCAAAGTCAAAATCAGGCGATTCTAAGCAGCGGCGCCCAAGAAACCAACTCCACGTTATCGCCAATAATACGTGGGAAAGTTCTCTAAGGGCATTGAGGAAATCCGCAATGGGTGCCGTGCTGCTGGCTTTATTAGTCATGTACAACCCGAAATCGAATTCGGCATTGGCTGCCCAGATCGGGCGGGTTGGGGGAAGCTGGTCTACCTGTTCCCCCCACTACATTATTAACCATGATGATCCGTATCCGGATGAAGTTACAGCTTGCATGTTATCTGTTCTGGCTATTTTGGCATGGAAGGATGTACGAACCAATGTAGTCAAGCTTCAGGTCCTTTTGTCAGACACGGGGCGAGCACTCCAAACGGATCTTAATCGGATTGCTGAAAGTGTAGATGGAGACACTTCTAGCTATAAGAGTTTGAGCTATATCTTGAATGAGACAGTTATAGCTTTGTTTCGGCGTCATGGTTACTACATCCCAGGTTATTCATCAGTGGCTTCACTGACCGGAGGTCGAGCTGCAAAGATTTACCTGAATTACCTTTCTATTAACGAGAGGATTCATTATAAAGAGACCCTTGTTAATGTAAACAACATTAAAGCACAAAGCTCGCCGAGCCATAGTTCAGCTAATGAATCGCAGAGTGAACATATAGTGGTTACAATATTGGTGGCTGCTAAAGGTCGGGATCACATACCCGCCATCTATGACAGTGATGGCTTGGAAAAAGTGTTGCACATACTTCTTTCCATTGGTCATTCAAACCGCATAAGAGTAAGAATGGAACTTCCTTTTGCAGTGGAAGCGTTGTGGACTCCTCACTTCTCTACGAGTGCGGCAAGTATCTGTAAATTACCTGCTCCCGAGGTGCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

366

Amino Acids

40.63

Weight (kDa)

9.54

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 177 - 348 9.3e-31 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018421)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23500
rosa_laevigata RLG00000007024
rosa_multiflora Rmu_sc0002225.1_g000013
rosa_roxburghii Rroxscaffold_5G00371730
rosa_rugosa Rorug02G0248500
rosa_samantha Rh4AG299900 Rh4BG307000 Rh4CG317300 Rh4DG298900
rosa_wichuraiana Rw4G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
Acc36I ACCTGC 1 cut(s) 1087
AccB1I GGYRCC 3 cut(s) 252, 332, 1090
AccI GTMKAC 1 cut(s) 422
AccIII TCCGGA 1 cut(s) 466
AciI CCGC 7 cut(s) 197, 250, 324, 403, 921, 988, 1058
AclWI GGATC 4 cut(s) 19, 452, 594, 918
AcsI RAATTY 3 cut(s) 44, 112, 376
AcuI CTGAAG 1 cut(s) 530
AcyI GRCGYC 2 cut(s) 253, 682
AfaI GTAC 2 cut(s) 362, 525
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 5 cut(s) 43, 49, 112, 655, 984
AhdI GACNNNNNGTC 1 cut(s) 732
AjnI CCWGG 1 cut(s) 700
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 3 cut(s) 612, 653, 788
AlwI GGATC 4 cut(s) 19, 452, 594, 918
AlwNI CAGNNNCTG 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 1085
Aor13HI TCCGGA 1 cut(s) 466
ApeKI GCWGC 7 cut(s) 11, 14, 247, 340, 389, 740, 896
ApoI RAATTY 3 cut(s) 44, 112, 376
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 151, 550
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 1085
AvaII GGWCC 2 cut(s) 151, 550
BanI GGYRCC 3 cut(s) 252, 332, 1090
BarI GAAGNNNNNNTAC 4 cut(s) 406, 438, 528, 560
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 6 cut(s) 23, 259, 327, 376, 727, 883
BccI CCATC 3 cut(s) 608, 932, 932
BceAI ACGGC 1 cut(s) 320
BcgI CGANNNNNNTGC 4 cut(s) 68, 102, 813, 847
BciT130I CCWGG 1 cut(s) 702
BciVI GTATCC 1 cut(s) 474
BcoDI GTCTC 3 cut(s) 612, 653, 788
BfaI CTAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 256
BfuAI ACCTGC 1 cut(s) 1087
BfuI GTATCC 1 cut(s) 474
BisI GCNGC 9 cut(s) 12, 15, 248, 251, 341, 390, 741, 897, 1059
BlsI GCNGC 9 cut(s) 13, 16, 249, 252, 342, 391, 742, 898, 1060
Bme1390I CCNGG 1 cut(s) 702
Bme18I GGWCC 2 cut(s) 151, 550
BmeRI GACNNNNNGTC 1 cut(s) 732
BmeT110I CYCGRG 1 cut(s) 1085
BmgT120I GGNCC 2 cut(s) 151, 550
BmiI GGNNCC 3 cut(s) 254, 334, 1092
BmrFI CCNGG 1 cut(s) 702
BsaAI YACGTR 1 cut(s) 291
BsaBI GATNNNNATC 2 cut(s) 462, 591
BsaHI GRCGYC 2 cut(s) 253, 682
BsaI GGTCTC 1 cut(s) 788
BsaJI CCNNGG 2 cut(s) 700, 1086
BsaWI WCCGGW 3 cut(s) 72, 466, 728
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse118I RCCGGY 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 333
Bse8I GATNNNNATC 2 cut(s) 462, 591
BseAI TCCGGA 1 cut(s) 466
BseBI CCWGG 1 cut(s) 702
BseDI CCNNGG 2 cut(s) 700, 1086
BseGI GGATG 3 cut(s) 475, 526, 696
BseJI GATNNNNATC 2 cut(s) 462, 591
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 1029
BseXI GCAGC 6 cut(s) 23, 259, 327, 376, 727, 883
BshNI GGYRCC 3 cut(s) 252, 332, 1090
BsiHKAI GWGCWC 1 cut(s) 577
BsiHKCI CYCGRG 1 cut(s) 1085
BsiSI CCGG 4 cut(s) 73, 194, 467, 729
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 3 cut(s) 612, 653, 788
Bso31I GGTCTC 1 cut(s) 788
BsoBI CYCGRG 1 cut(s) 1085
Bsp1286I GDGCHC 1 cut(s) 577
Bsp13I TCCGGA 1 cut(s) 466
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 5 cut(s) 24, 396, 457, 586, 910
BspACI CCGC 7 cut(s) 197, 250, 324, 403, 921, 988, 1058
BspCNI CTCAG 1 cut(s) 194
BspEI TCCGGA 1 cut(s) 466
BspLI GGNNCC 3 cut(s) 254, 334, 1092
BspMI ACCTGC 1 cut(s) 1087
BspPI GGATC 4 cut(s) 19, 452, 594, 918
BspT107I GGYRCC 3 cut(s) 252, 332, 1090
BspTNI GGTCTC 1 cut(s) 788
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 193
BsrGI TGTACA 1 cut(s) 360
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 700, 1086
BssMI GATC 5 cut(s) 24, 396, 457, 586, 910
BssNI GRCGYC 2 cut(s) 253, 682
Bst2UI CCWGG 1 cut(s) 702
Bst4CI ACNGT 2 cut(s) 664, 935
Bst6I CTCTTC 1 cut(s) 90
BstACI GRCGYC 2 cut(s) 253, 682
BstAPI GCANNNNNTGC 2 cut(s) 332, 389
BstAUI TGTACA 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 291
BstC8I GCNNGC 5 cut(s) 195, 345, 484, 573, 833
BstDEI CTNAG 3 cut(s) 181, 243, 305
BstF5I GGATG 3 cut(s) 475, 526, 696
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 5 cut(s) 27, 399, 460, 589, 913
BstMAI GTCTC 3 cut(s) 612, 653, 788
BstMBI GATC 5 cut(s) 24, 396, 457, 586, 910
BstMWI GCNNNNNNNGC 6 cut(s) 11, 332, 389, 509, 837, 1025
BstNI CCWGG 1 cut(s) 702
BstNSI RCATGY 1 cut(s) 490
BstSCI CCNGG 1 cut(s) 700
BstV1I GCAGC 6 cut(s) 23, 259, 327, 376, 727, 883
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuI GTATCC 1 cut(s) 474
BtsCI GGATG 3 cut(s) 475, 526, 696
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 4 cut(s) 720, 722, 940, 1026
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 5 cut(s) 195, 345, 484, 573, 833
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 1 cut(s) 255
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 151, 550
CseI GACGC 1 cut(s) 671
Csp6I GTAC 2 cut(s) 361, 524
CspCI CAANNNNNGTGG 2 cut(s) 873, 908
CviAII CATG 5 cut(s) 358, 452, 487, 513, 686
CviQI GTAC 2 cut(s) 361, 524
DdeI CTNAG 3 cut(s) 181, 243, 305
DinI GGCGCC 1 cut(s) 254
DpnI GATC 5 cut(s) 26, 398, 459, 588, 912
DpnII GATC 5 cut(s) 24, 396, 457, 586, 910
DraI TTTAAA 1 cut(s) 177
DriI GACNNNNNGTC 1 cut(s) 732
Eam1104I CTCTTC 1 cut(s) 90
Eam1105I GACNNNNNGTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 90
EciI GGCGGA 1 cut(s) 212
Eco31I GGTCTC 1 cut(s) 788
Eco47I GGWCC 2 cut(s) 151, 550
Eco57I CTGAAG 1 cut(s) 530
Eco88I CYCGRG 1 cut(s) 1085
EcoO109I RGGNCCY 2 cut(s) 151, 550
EcoRI GAATTC 2 cut(s) 112, 376
EcoRII CCWGG 1 cut(s) 700
EgeI GGCGCC 1 cut(s) 254
EheI GGCGCC 1 cut(s) 254
FaeI CATG 5 cut(s) 361, 455, 490, 516, 689
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FatI CATG 5 cut(s) 357, 451, 486, 512, 685
FauI CCCGC 2 cut(s) 396, 928
FblI GTMKAC 1 cut(s) 422
Fnu4HI GCNGC 9 cut(s) 12, 15, 248, 251, 341, 390, 741, 897, 1059
FokI GGATG 3 cut(s) 482, 533, 683
Fsp4HI GCNGC 9 cut(s) 12, 15, 248, 251, 341, 390, 741, 897, 1059
FspBI CTAG 1 cut(s) 627
GlaI GCGC 1 cut(s) 254
GluI GCNGC 9 cut(s) 12, 15, 248, 251, 341, 390, 741, 897, 1059
HaeII RGCGCY 1 cut(s) 256
HapII CCGG 4 cut(s) 73, 194, 467, 729
HgaI GACGC 1 cut(s) 671
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 2 cut(s) 253, 682
Hin1II CATG 5 cut(s) 361, 455, 490, 516, 689
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 5 cut(s) 218, 239, 781, 857, 1036
HpaII CCGG 4 cut(s) 73, 194, 467, 729
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 4 cut(s) 423, 811, 869, 1035
Hpy188I TCNGA 3 cut(s) 184, 562, 597
Hpy188III TCNNGA 4 cut(s) 467, 652, 908, 1085
Hpy8I GTNNAC 4 cut(s) 423, 811, 869, 1035
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 2 cut(s) 142, 511
HpyCH4III ACNGT 2 cut(s) 664, 935
HpyCH4IV ACGT 2 cut(s) 273, 290
HpyCH4V TGCA 5 cut(s) 79, 486, 743, 958, 1019
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 332, 389, 509, 837, 1025
HpyF3I CTNAG 3 cut(s) 181, 243, 305
HpySE526I ACGT 2 cut(s) 273, 290
Hsp92I GRCGYC 2 cut(s) 253, 682
Hsp92II CATG 5 cut(s) 361, 455, 490, 516, 689
HspAI GCGC 1 cut(s) 253
KasI GGCGCC 1 cut(s) 252
Kpn2I TCCGGA 1 cut(s) 466
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Kzo9I GATC 5 cut(s) 24, 396, 457, 586, 910
LmnI GCTCC 1 cut(s) 1087
Lsp1109I GCAGC 6 cut(s) 23, 259, 327, 376, 727, 883
MaeI CTAG 1 cut(s) 627
MaeII ACGT 2 cut(s) 273, 290
MaeIII GTNAC 3 cut(s) 475, 689, 880
MalI GATC 5 cut(s) 26, 398, 459, 588, 912
MboI GATC 5 cut(s) 24, 396, 457, 586, 910
MboII GAAGA 3 cut(s) 77, 116, 199
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 5 cut(s) 44, 112, 376, 757, 1073
Mly113I GGCGCC 1 cut(s) 253
MlyI GAGTC 2 cut(s) 227, 1030
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 6 cut(s) 93, 309, 725, 771, 1050, 1081
MroI TCCGGA 1 cut(s) 466
MroNI GCCGGC 1 cut(s) 193
MseI TTAA 8 cut(s) 156, 162, 176, 447, 591, 771, 804, 819
MspA1I CMGCKG 2 cut(s) 14, 250
MspI CCGG 4 cut(s) 73, 194, 467, 729
MspR9I CCNGG 1 cut(s) 702
MvaI CCWGG 1 cut(s) 702
MwoI GCNNNNNNNGC 6 cut(s) 11, 332, 389, 509, 837, 1025
NaeI GCCGGC 1 cut(s) 195
NarI GGCGCC 1 cut(s) 253
NdeII GATC 5 cut(s) 24, 396, 457, 586, 910
NgoMIV GCCGGC 1 cut(s) 193
NlaIII CATG 5 cut(s) 361, 455, 490, 516, 689
NlaIV GGNNCC 3 cut(s) 254, 334, 1092
NmeAIII GCCGAG 1 cut(s) 861
NspI RCATGY 1 cut(s) 490
PdiI GCCGGC 1 cut(s) 195
PfeI GAWTC 3 cut(s) 239, 781, 857
PkrI GCNGC 9 cut(s) 13, 16, 249, 252, 342, 391, 742, 898, 1060
PleI GAGTC 2 cut(s) 226, 1030
PluTI GGCGCC 1 cut(s) 256
PpsI GAGTC 2 cut(s) 226, 1030
Ppu21I YACGTR 1 cut(s) 291
PpuMI RGGWCCY 2 cut(s) 151, 550
PsiI TTATAA 1 cut(s) 789
Psp5II RGGWCCY 2 cut(s) 151, 550
Psp6I CCWGG 1 cut(s) 700
PspGI CCWGG 1 cut(s) 700
PspN4I GGNNCC 3 cut(s) 254, 334, 1092
PspPI GGNCC 2 cut(s) 151, 550
PspPPI RGGWCCY 2 cut(s) 151, 550
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 2 cut(s) 362, 525
RsaNI GTAC 2 cut(s) 361, 524
SaqAI TTAA 8 cut(s) 156, 162, 176, 447, 591, 771, 804, 819
SatI GCNGC 9 cut(s) 12, 15, 248, 251, 341, 390, 741, 897, 1059
Sau3AI GATC 5 cut(s) 24, 396, 457, 586, 910
Sau96I GGNCC 2 cut(s) 151, 550
SchI GAGTC 2 cut(s) 227, 1030
ScrFI CCNGG 1 cut(s) 702
SduI GDGCHC 1 cut(s) 577
SfoI GGCGCC 1 cut(s) 254
SinI GGWCC 2 cut(s) 151, 550
Sse9I AATT 5 cut(s) 44, 112, 376, 757, 1073
SsiI CCGC 7 cut(s) 197, 250, 324, 403, 921, 988, 1058
SspDI GGCGCC 1 cut(s) 252
SspI AATATT 1 cut(s) 888
SspMI CTAG 1 cut(s) 627
StyD4I CCNGG 1 cut(s) 700
TaaI ACNGT 2 cut(s) 664, 935
TaiI ACGT 2 cut(s) 276, 293
TaqI TCGA 2 cut(s) 374, 736
TasI AATT 5 cut(s) 44, 112, 376, 757, 1073
TatI WGTACW 1 cut(s) 360
TauI GCSGC 2 cut(s) 253, 1061
TfiI GAWTC 3 cut(s) 239, 781, 857
Tru1I TTAA 8 cut(s) 156, 162, 176, 447, 591, 771, 804, 819
Tru9I TTAA 8 cut(s) 156, 162, 176, 447, 591, 771, 804, 819
TscAI CASTG 4 cut(s) 720, 729, 940, 1026
TseI GCWGC 7 cut(s) 11, 14, 247, 340, 389, 740, 896
TspDTI ATGAA 4 cut(s) 486, 699, 773, 870
TspGWI ACGGA 3 cut(s) 160, 450, 599
TspRI CASTG 4 cut(s) 720, 729, 940, 1026
VpaK11BI GGWCC 2 cut(s) 151, 550
XapI RAATTY 3 cut(s) 44, 112, 376
XceI RCATGY 1 cut(s) 490
XmiI GTMKAC 1 cut(s) 422
XspI CTAG 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.