Rmu_sc0002225.1_g000013

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002225.1
OG0018421
Physical Location & Seq
Forward (+)
65429 .. 70547
5119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002225.1_g000013.1.cds

Sequence Viewer

Length: 1305 bp
atggcaacagcagctgcgttggtgatccaagccaactcattcaaattcaaatggaacgaagcccacgcaatccggttgcaccctcttcgtcgttccaaagacattagcttgaattccaacaatcttcgttcttgttctccaatacggaaggaccttaaacttaaatgcttcttcttaaactcagagaagaagccggcggactactttaccaccaccgagtcaaagtcaaaatcaggcgattctaagcggcggccaagaaaccaactccacgttatcgccaataatacgtgggaaagttctctaagggcattgaggaaatccgcaatgggtgccgtgctgctggctttattagtcatgtacaacccgaaatcgaattcggcattggctgcccagatcgggcgggttgggggaagctggtctacctgttccccccactacattattgaccatgatgatccgtatccggaggatgttacagcttgttatgttttctgcttcattcttgcgggtttttttgtggtggttacttctgcttttggtgatgcttcgtggatggaattgaaattgatcggtgtggcagctgttctggctattttggcatggaaagatgtacgaaccaatgtagtcaagcttcaggttcgtttgtcagacacggggcaagcactccaaacggatcttaatcggattgctgaaagtgtagatggagacacttctagctataggagtttgagctatatcttgaatgagacagttatagctttgtttcggcgtcatggttactacatctcaggttattcatcagtggcttcactggccggaggtcgagctgcaaagatttacctgaattacctttctattaacgagaggattcatcataaagagacgcttgttaatgtaaacaacattaaagcccaaagcttgccgagccatagcagtggaagtgttgtggactcatcacttctctacgagtgcggcaagtatctgggtagcataaaaccgtcgcagagtaagtgtcgcaaaactctttgccgtcgcaaaagtggtctgctacggcaaaatgccgtggcaaaagatattggcgacgccaccaacggcgacggaagctttgccgtcgcagagccgtcgcaaatgcttttttgctacggcaaaatagcttttcgctacggcaaggcgccttcggtggttggctggctggccgaaggagctgcaggggcagcggagacgcacggctgtccaagaggactgcaggggcaggctaggacgtgtggtggtgctgtaggggcagcaggagtgcggcaaggctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

434

Amino Acids

47.14

Weight (kDa)

9.59

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018421)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23500
rosa_laevigata RLG00000007024
rosa_multiflora Rmu_sc0002225.1_g000013
rosa_roxburghii Rroxscaffold_5G00371730
rosa_rugosa Rorug02G0248500
rosa_samantha Rh4AG299900 Rh4BG307000 Rh4CG317300 Rh4DG298900
rosa_wichuraiana Rw4G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 329, 1171
AccI GTMKAC 1 cut(s) 419
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 9 cut(s) 197, 247, 250, 321, 400, 506, 972, 1217, 1294
AclWI GGATC 3 cut(s) 19, 449, 681
AcoI YGGCCR 3 cut(s) 251, 813, 1194
AcsI RAATTY 3 cut(s) 44, 112, 373
AcuI CTGAAG 1 cut(s) 617
AcyI GRCGYC 3 cut(s) 769, 1083, 1172
AfaI GTAC 2 cut(s) 359, 612
AfiI CCNNNNNNNGG 1 cut(s) 396
AflIII ACRYGT 1 cut(s) 1262
AgsI TTSAA 5 cut(s) 43, 49, 112, 562, 742
AjiI CACGTC 1 cut(s) 1263
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
Alw26I GTCTC 4 cut(s) 699, 740, 875, 1214
AlwI GGATC 3 cut(s) 19, 449, 681
AlwNI CAGNNNCTG 1 cut(s) 14
Aor13HI TCCGGA 1 cut(s) 463
AoxI GGCC 3 cut(s) 251, 813, 1194
ApeKI GCWGC 9 cut(s) 11, 14, 337, 386, 578, 827, 1205, 1214, 1283
ApoI RAATTY 3 cut(s) 44, 112, 373
ArsI GACNNNNNNTTYG 2 cut(s) 1088, 1120
AspLEI GCGC 1 cut(s) 1174
AspS9I GGNCC 1 cut(s) 151
AsuHPI GGTGA 2 cut(s) 34, 551
AvaII GGWCC 1 cut(s) 151
BanI GGYRCC 2 cut(s) 329, 1171
BarI GAAGNNNNNNTAC 4 cut(s) 403, 435, 615, 647
BbvI GCAGC 8 cut(s) 23, 324, 373, 590, 814, 1192, 1226, 1295
BccI CCATC 2 cut(s) 547, 695
BcgI CGANNNNNNTGC 6 cut(s) 68, 102, 1104, 1138, 1187, 1221
BciVI GTATCC 1 cut(s) 471
BcoDI GTCTC 4 cut(s) 699, 740, 875, 1214
BfaI CTAG 3 cut(s) 714, 1257, 1303
BfmI CTRYAG 4 cut(s) 718, 1206, 1244, 1275
BfoI RGCGCY 1 cut(s) 1175
BfuI GTATCC 1 cut(s) 471
Bme18I GGWCC 1 cut(s) 151
BmgBI CACGTC 1 cut(s) 1263
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 331, 1173
BmsI GCATC 1 cut(s) 532
BsaAI YACGTR 1 cut(s) 288
BsaBI GATNNNNATC 2 cut(s) 459, 678
BsaHI GRCGYC 3 cut(s) 769, 1083, 1172
BsaJI CCNNGG 1 cut(s) 1062
BsaWI WCCGGW 2 cut(s) 72, 463
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 816
Bse3DI GCAATG 1 cut(s) 330
Bse8I GATNNNNATC 2 cut(s) 459, 678
BseAI TCCGGA 1 cut(s) 463
BseDI CCNNGG 1 cut(s) 1062
BseGI GGATG 2 cut(s) 475, 558
BseJI GATNNNNATC 2 cut(s) 459, 678
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 2 cut(s) 195, 801
BseNI ACTGG 1 cut(s) 816
BseXI GCAGC 8 cut(s) 23, 324, 373, 590, 814, 1192, 1226, 1295
BshFI GGCC 3 cut(s) 253, 815, 1196
BshNI GGYRCC 2 cut(s) 329, 1171
BsiSI CCGG 4 cut(s) 73, 194, 464, 816
BslI CCNNNNNNNGG 1 cut(s) 396
BsmAI GTCTC 4 cut(s) 699, 740, 875, 1214
BsmBI CGTCTC 2 cut(s) 875, 1214
BsnI GGCC 3 cut(s) 253, 815, 1196
Bsp13I TCCGGA 1 cut(s) 463
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 5 cut(s) 24, 393, 454, 567, 673
BspACI CCGC 9 cut(s) 197, 247, 250, 321, 400, 506, 972, 1217, 1294
BspANI GGCC 3 cut(s) 253, 815, 1196
BspCNI CTCAG 2 cut(s) 194, 800
BspEI TCCGGA 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 331, 1173
BspMAI CTGCAG 2 cut(s) 1210, 1248
BspPI GGATC 3 cut(s) 19, 449, 681
BspT107I GGYRCC 2 cut(s) 329, 1171
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 193
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 1 cut(s) 816
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 1062
BssMI GATC 5 cut(s) 24, 393, 454, 567, 673
BssNI GRCGYC 3 cut(s) 769, 1083, 1172
Bst4CI ACNGT 2 cut(s) 751, 999
Bst6I CTCTTC 1 cut(s) 90
BstACI GRCGYC 3 cut(s) 769, 1083, 1172
BstAPI GCANNNNNTGC 2 cut(s) 329, 386
BstAUI TGTACA 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 288
BstC8I GCNNGC 7 cut(s) 195, 342, 660, 920, 1190, 1194, 1254
BstDEI CTNAG 4 cut(s) 181, 243, 302, 787
BstDSI CCRYGG 1 cut(s) 1062
BstF5I GGATG 2 cut(s) 475, 558
BstH2I RGCGCY 1 cut(s) 1175
BstHHI GCGC 1 cut(s) 1174
BstKTI GATC 5 cut(s) 27, 396, 457, 570, 676
BstMAI GTCTC 4 cut(s) 699, 740, 875, 1214
BstMBI GATC 5 cut(s) 24, 393, 454, 567, 673
BstSFI CTRYAG 4 cut(s) 718, 1206, 1244, 1275
BstV1I GCAGC 8 cut(s) 23, 324, 373, 590, 814, 1192, 1226, 1295
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 1 cut(s) 935
BstYI RGATCY 1 cut(s) 673
BsuI GTATCC 1 cut(s) 471
BsuRI GGCC 3 cut(s) 253, 815, 1196
BtgI CCRYGG 1 cut(s) 1062
BtrI CACGTC 1 cut(s) 1263
BtsCI GGATG 2 cut(s) 475, 558
BtsI GCAGTG 1 cut(s) 940
BtsIMutI CAGTG 3 cut(s) 807, 809, 940
Cac8I GCNNGC 7 cut(s) 195, 342, 660, 920, 1190, 1194, 1254
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 1 cut(s) 1174
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 151
CseI GACGC 4 cut(s) 758, 892, 1091, 1231
Csp6I GTAC 2 cut(s) 358, 611
CviAII CATG 4 cut(s) 355, 449, 600, 773
CviQI GTAC 2 cut(s) 358, 611
DdeI CTNAG 4 cut(s) 181, 243, 302, 787
DinI GGCGCC 1 cut(s) 1173
DpnI GATC 5 cut(s) 26, 395, 456, 569, 675
DpnII GATC 5 cut(s) 24, 393, 454, 567, 673
EaeI YGGCCR 3 cut(s) 251, 813, 1194
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
EciI GGCGGA 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 151
EcoRI GAATTC 2 cut(s) 112, 373
EgeI GGCGCC 1 cut(s) 1173
EheI GGCGCC 1 cut(s) 1173
Esp3I CGTCTC 2 cut(s) 875, 1214
FaeI CATG 4 cut(s) 358, 452, 603, 776
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FatI CATG 4 cut(s) 354, 448, 599, 772
FauI CCCGC 2 cut(s) 393, 499
FblI GTMKAC 1 cut(s) 419
FokI GGATG 2 cut(s) 482, 565
FspBI CTAG 3 cut(s) 714, 1257, 1303
GlaI GCGC 1 cut(s) 1173
HaeII RGCGCY 1 cut(s) 1175
HaeIII GGCC 3 cut(s) 253, 815, 1196
HapII CCGG 4 cut(s) 73, 194, 464, 816
HgaI GACGC 4 cut(s) 758, 892, 1091, 1231
HhaI GCGC 1 cut(s) 1174
Hin1I GRCGYC 3 cut(s) 769, 1083, 1172
Hin1II CATG 4 cut(s) 358, 452, 603, 776
Hin6I GCGC 1 cut(s) 1172
HinP1I GCGC 1 cut(s) 1172
HindIII AAGCTT 3 cut(s) 629, 916, 1102
HinfI GANTC 4 cut(s) 218, 239, 868, 950
HpaII CCGG 4 cut(s) 73, 194, 464, 816
HphI GGTGA 2 cut(s) 34, 551
Hpy166II GTNNAC 3 cut(s) 420, 898, 949
Hpy188I TCNGA 3 cut(s) 184, 649, 684
Hpy188III TCNNGA 2 cut(s) 464, 739
Hpy8I GTNNAC 3 cut(s) 420, 898, 949
Hpy99I CGWCG 7 cut(s) 93, 1003, 1035, 1085, 1100, 1115, 1126
HpyAV CCTTC 3 cut(s) 142, 1185, 1193
HpyCH4III ACNGT 2 cut(s) 751, 999
HpyCH4IV ACGT 3 cut(s) 270, 287, 1262
HpyCH4V TGCA 4 cut(s) 79, 830, 1208, 1246
HpyF3I CTNAG 4 cut(s) 181, 243, 302, 787
HpySE526I ACGT 3 cut(s) 270, 287, 1262
Hsp92I GRCGYC 3 cut(s) 769, 1083, 1172
Hsp92II CATG 4 cut(s) 358, 452, 603, 776
HspAI GCGC 1 cut(s) 1172
KasI GGCGCC 1 cut(s) 1171
Kpn2I TCCGGA 1 cut(s) 463
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Kzo9I GATC 5 cut(s) 24, 393, 454, 567, 673
LmnI GCTCC 1 cut(s) 1202
Lsp1109I GCAGC 8 cut(s) 23, 324, 373, 590, 814, 1192, 1226, 1295
LweI GCATC 1 cut(s) 532
MaeI CTAG 3 cut(s) 714, 1257, 1303
MaeII ACGT 3 cut(s) 270, 287, 1262
MaeIII GTNAC 3 cut(s) 472, 523, 776
MalI GATC 5 cut(s) 26, 395, 456, 569, 675
MboI GATC 5 cut(s) 24, 393, 454, 567, 673
MboII GAAGA 4 cut(s) 77, 116, 163, 199
MflI RGATCY 1 cut(s) 673
MluCI AATT 6 cut(s) 44, 112, 373, 557, 563, 844
Mly113I GGCGCC 1 cut(s) 1172
MlyI GAGTC 2 cut(s) 227, 944
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 6 cut(s) 93, 306, 460, 812, 858, 1232
MroI TCCGGA 1 cut(s) 463
MroNI GCCGGC 1 cut(s) 193
MseI TTAA 7 cut(s) 156, 162, 176, 678, 858, 891, 906
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 3 cut(s) 14, 581, 1217
MspI CCGG 4 cut(s) 73, 194, 464, 816
NaeI GCCGGC 1 cut(s) 195
NarI GGCGCC 1 cut(s) 1172
NdeII GATC 5 cut(s) 24, 393, 454, 567, 673
NgoMIV GCCGGC 1 cut(s) 193
NlaIII CATG 4 cut(s) 358, 452, 603, 776
NlaIV GGNNCC 2 cut(s) 331, 1173
NmeAIII GCCGAG 1 cut(s) 948
PdiI GCCGGC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 239, 868
PleI GAGTC 2 cut(s) 226, 944
PluTI GGCGCC 1 cut(s) 1175
PpsI GAGTC 2 cut(s) 226, 944
Ppu21I YACGTR 1 cut(s) 288
PpuMI RGGWCCY 1 cut(s) 151
Psp5II RGGWCCY 1 cut(s) 151
PspN4I GGNNCC 2 cut(s) 331, 1173
PspPI GGNCC 1 cut(s) 151
PspPPI RGGWCCY 1 cut(s) 151
PstI CTGCAG 2 cut(s) 1210, 1248
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 2 cut(s) 14, 581
RsaI GTAC 2 cut(s) 359, 612
RsaNI GTAC 2 cut(s) 358, 611
RseI CAYNNNNRTG 1 cut(s) 933
SaqAI TTAA 7 cut(s) 156, 162, 176, 678, 858, 891, 906
Sau3AI GATC 5 cut(s) 24, 393, 454, 567, 673
Sau96I GGNCC 1 cut(s) 151
SchI GAGTC 2 cut(s) 227, 944
SfaNI GCATC 1 cut(s) 532
SfcI CTRYAG 4 cut(s) 718, 1206, 1244, 1275
SfoI GGCGCC 1 cut(s) 1173
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 933
Sse9I AATT 6 cut(s) 44, 112, 373, 557, 563, 844
SsiI CCGC 9 cut(s) 197, 247, 250, 321, 400, 506, 972, 1217, 1294
SspDI GGCGCC 1 cut(s) 1171
SspMI CTAG 3 cut(s) 714, 1257, 1303
TaaI ACNGT 2 cut(s) 751, 999
TaiI ACGT 3 cut(s) 273, 290, 1265
TaqI TCGA 2 cut(s) 371, 823
TasI AATT 6 cut(s) 44, 112, 373, 557, 563, 844
TatI WGTACW 1 cut(s) 357
TauI GCSGC 4 cut(s) 250, 253, 975, 1297
TfiI GAWTC 2 cut(s) 239, 868
Tru1I TTAA 7 cut(s) 156, 162, 176, 678, 858, 891, 906
Tru9I TTAA 7 cut(s) 156, 162, 176, 678, 858, 891, 906
TscAI CASTG 3 cut(s) 807, 816, 940
TseI GCWGC 9 cut(s) 11, 14, 337, 386, 578, 827, 1205, 1214, 1283
TspDTI ATGAA 3 cut(s) 487, 786, 860
TspGWI ACGGA 4 cut(s) 160, 447, 686, 1113
TspRI CASTG 3 cut(s) 807, 816, 940
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 3 cut(s) 44, 112, 373
XmiI GTMKAC 1 cut(s) 419
XspI CTAG 3 cut(s) 714, 1257, 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.