Rh4AG299900

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018421
Physical Location & Seq
Reverse (-)
61270547 .. 61290294
19748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG299900.1

Sequence Viewer

Length: 1014 bp
ATGGAAAAGGTCCTGACTGAATCTGAGAAGAAGCCGGCGGACTACTTAACCACCACCGAGTCAAAGTCAAAATCAGGAGAGTCTCAGTGGCGTCCAAGAAACCAAATCCACATTAGCGCCAATAATATGTGGGAAAGTTCTCTTAGGGCATTAAGGAAATCCGCAATCGGTGCCGTACTGCTGGGTTTATTAGTCGTGTACAACCCGAAATCGAATTCGGCATTGGCTGCCCCGGTCGGGCGGGTTGGGGGAAGCTGGTCTACCTGGTCACCATCGTACTCTATATATGTATATAGCCACTACAATATTAACCATGATAACCATGATGATCCGTATCCGGCTGGAGTTGCAGCTTGTTATTTTTTCTACTTCATTGTTGCGGCTTTTTTTGTGGTGGCTAGTTCTGGTTTTGATGATGTTTCAGTTTCGTGGTTGAAATTGAAATTGATCGGTTTGGCAGTTGTTCTAGCTACTTTGGCTTGGTGTGATGTACAAACCAATGTAGTCAAGCTTCAGACGGATCTTAATCGGATTGCTGAAAGTGTACATGGAGACACTTCTAGCTATAAGAGTTTGAGCTATGTCTTGAATGAGACAGTAATAGCTTTGTTTCGGCGCCATGGTTACTACATCTCAGGTTATTCATCAGTGGCTTCACAGACCGGAGGTCGAGCTGCAAAGATTTACCTGAATTACCTTTCTATTAACGAGAGGATTCATCAAAAAGAGACGCTTGTTAATGTAAACAACATGAAAGCAGGAAGCTCGCCGAGCCATAGTCCAGCTAATGAATCGCAGAATGAACATGTAGTGGTTACAATATTGGTAGCTGCTAAAGGTTGGGATCACATACCCGCCATCTATGACAGTCATAGCTTGGAAAAAGTGTTGCACATACTTCTTTCCATTGGTCATTCAAACCGCATAAGAGCAGTGGAAGTGTTGTGGACTCCTCAGTTCTTTTCGAGTGCGGCAAGTATCTGTAAATTACCCGCTTCCGAGGTGCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

337

Amino Acids

37.17

Weight (kDa)

8.58

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 169 - 319 8.6e-30 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018421)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23500
rosa_laevigata RLG00000007024
rosa_multiflora Rmu_sc0002225.1_g000013
rosa_roxburghii Rroxscaffold_5G00371730
rosa_rugosa Rorug02G0248500
rosa_samantha Rh4AG299900 Rh4BG307000 Rh4CG317300 Rh4DG298900
rosa_wichuraiana Rw4G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 170, 615, 1003
AccI GTMKAC 1 cut(s) 260
AciI CCGC 8 cut(s) 38, 162, 241, 380, 855, 922, 971, 993
AclWI GGATC 3 cut(s) 323, 528, 852
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 497
AcyI GRCGYC 2 cut(s) 91, 616
AfaI GTAC 5 cut(s) 177, 200, 278, 492, 546
AfiI CCNNNNNNNGG 1 cut(s) 237
AflIII ACRYGT 1 cut(s) 805
AgsI TTSAA 4 cut(s) 436, 442, 589, 918
AhdI GACNNNNNGTC 1 cut(s) 666
AjnI CCWGG 1 cut(s) 263
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
Alw26I GTCTC 4 cut(s) 87, 546, 587, 722
AlwI GGATC 3 cut(s) 323, 528, 852
ApeKI GCWGC 4 cut(s) 227, 350, 674, 830
ApoI RAATTY 1 cut(s) 214
AspLEI GCGC 2 cut(s) 119, 618
AspS9I GGNCC 1 cut(s) 10
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 261
AvaII GGWCC 1 cut(s) 10
BanI GGYRCC 3 cut(s) 170, 615, 1003
BarI GAAGNNNNNNTAC 4 cut(s) 244, 276, 495, 527
BbvI GCAGC 4 cut(s) 214, 362, 661, 817
BccI CCATC 2 cut(s) 280, 866
BceAI ACGGC 1 cut(s) 158
BcgI CGANNNNNNTGC 2 cut(s) 747, 781
BciT130I CCWGG 1 cut(s) 265
BciVI GTATCC 1 cut(s) 345
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 4 cut(s) 87, 546, 587, 722
BfaI CTAG 3 cut(s) 399, 467, 561
BfoI RGCGCY 2 cut(s) 120, 619
BfuI GTATCC 1 cut(s) 345
BisI GCNGC 6 cut(s) 228, 351, 381, 675, 831, 972
BlsI GCNGC 6 cut(s) 229, 352, 382, 676, 832, 973
Bme1390I CCNGG 2 cut(s) 233, 265
Bme18I GGWCC 1 cut(s) 10
BmeRI GACNNNNNGTC 1 cut(s) 666
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 3 cut(s) 172, 617, 1005
BmrFI CCNGG 2 cut(s) 233, 265
BpmI CTGGAG 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 233
BsaBI GATNNNNATC 2 cut(s) 333, 525
BsaHI GRCGYC 2 cut(s) 91, 616
BsaJI CCNNGG 3 cut(s) 231, 619, 999
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 1 cut(s) 237
Bse118I RCCGGY 1 cut(s) 34
Bse8I GATNNNNATC 2 cut(s) 333, 525
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 3 cut(s) 231, 619, 999
BseJI GATNNNNATC 2 cut(s) 333, 525
BseLI CCNNNNNNNGG 1 cut(s) 237
BseMII CTCAG 4 cut(s) 15, 98, 648, 968
BseRI GAGGAG 1 cut(s) 942
BseXI GCAGC 4 cut(s) 214, 362, 661, 817
BseYI CCCAGC 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 237
BshNI GGYRCC 3 cut(s) 170, 615, 1003
BsiEI CGRYCG 1 cut(s) 237
BsiSI CCGG 4 cut(s) 35, 233, 338, 663
BslI CCNNNNNNNGG 1 cut(s) 237
BsmAI GTCTC 4 cut(s) 87, 546, 587, 722
BsmBI CGTCTC 1 cut(s) 722
Bsp1407I TGTACA 3 cut(s) 198, 490, 544
Bsp143I GATC 4 cut(s) 328, 447, 520, 844
Bsp19I CCATGG 1 cut(s) 619
BspACI CCGC 8 cut(s) 38, 162, 241, 380, 855, 922, 971, 993
BspCNI CTCAG 4 cut(s) 16, 97, 647, 967
BspLI GGNNCC 3 cut(s) 172, 617, 1005
BspPI GGATC 3 cut(s) 323, 528, 852
BspT107I GGYRCC 3 cut(s) 170, 615, 1003
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 3 cut(s) 198, 490, 544
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 231, 619, 999
BssMI GATC 4 cut(s) 328, 447, 520, 844
BssNI GRCGYC 2 cut(s) 91, 616
BssT1I CCWWGG 1 cut(s) 619
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 2 cut(s) 598, 869
BstACI GRCGYC 2 cut(s) 91, 616
BstAPI GCANNNNNTGC 2 cut(s) 170, 227
BstAUI TGTACA 3 cut(s) 198, 490, 544
BstC8I GCNNGC 2 cut(s) 36, 767
BstDEI CTNAG 5 cut(s) 24, 84, 143, 634, 954
BstDSI CCRYGG 1 cut(s) 619
BstEII GGTNACC 1 cut(s) 267
BstH2I RGCGCY 2 cut(s) 120, 619
BstHHI GCGC 2 cut(s) 119, 618
BstKTI GATC 4 cut(s) 331, 450, 523, 847
BstMAI GTCTC 4 cut(s) 87, 546, 587, 722
BstMBI GATC 4 cut(s) 328, 447, 520, 844
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 5 cut(s) 170, 227, 347, 476, 771
BstNI CCWGG 1 cut(s) 265
BstNSI RCATGY 1 cut(s) 809
BstPI GGTNACC 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 231, 263
BstV1I GCAGC 4 cut(s) 214, 362, 661, 817
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BsuI GTATCC 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 3 cut(s) 92, 654, 939
Cac8I GCNNGC 2 cut(s) 36, 767
CfoI GCGC 2 cut(s) 119, 618
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 10
CseI GACGC 2 cut(s) 80, 739
CsiI ACCWGGT 1 cut(s) 263
Csp6I GTAC 5 cut(s) 176, 199, 277, 491, 545
CviAII CATG 6 cut(s) 314, 323, 548, 620, 751, 806
CviQI GTAC 5 cut(s) 176, 199, 277, 491, 545
DdeI CTNAG 5 cut(s) 24, 84, 143, 634, 954
DinI GGCGCC 1 cut(s) 617
DpnI GATC 4 cut(s) 330, 449, 522, 846
DpnII GATC 4 cut(s) 328, 447, 520, 844
DriI GACNNNNNGTC 1 cut(s) 666
Eam1105I GACNNNNNGTC 1 cut(s) 666
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 619
EgeI GGCGCC 1 cut(s) 617
EheI GGCGCC 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 619
Esp3I CGTCTC 1 cut(s) 722
FaeI CATG 6 cut(s) 317, 326, 551, 623, 754, 809
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FatI CATG 6 cut(s) 313, 322, 547, 619, 750, 805
FauI CCCGC 3 cut(s) 234, 862, 1000
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 6 cut(s) 228, 351, 381, 675, 831, 972
Fsp4HI GCNGC 6 cut(s) 228, 351, 381, 675, 831, 972
FspBI CTAG 3 cut(s) 399, 467, 561
GlaI GCGC 2 cut(s) 118, 617
GluI GCNGC 6 cut(s) 228, 351, 381, 675, 831, 972
GsaI CCCAGC 1 cut(s) 185
GsuI CTGGAG 1 cut(s) 363
HaeII RGCGCY 2 cut(s) 120, 619
HapII CCGG 4 cut(s) 35, 233, 338, 663
HgaI GACGC 2 cut(s) 80, 739
HhaI GCGC 2 cut(s) 119, 618
Hin1I GRCGYC 2 cut(s) 91, 616
Hin1II CATG 6 cut(s) 317, 326, 551, 623, 754, 809
Hin6I GCGC 2 cut(s) 117, 616
HinP1I GCGC 2 cut(s) 117, 616
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 6 cut(s) 20, 59, 80, 715, 791, 949
HpaII CCGG 4 cut(s) 35, 233, 338, 663
HphI GGTGA 1 cut(s) 261
Hpy166II GTNNAC 5 cut(s) 199, 261, 545, 745, 948
Hpy188I TCNGA 4 cut(s) 25, 516, 531, 1000
Hpy188III TCNNGA 3 cut(s) 13, 75, 586
Hpy8I GTNNAC 5 cut(s) 199, 261, 545, 745, 948
HpyCH4III ACNGT 2 cut(s) 598, 869
HpyCH4V TGCA 3 cut(s) 350, 677, 892
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 227, 347, 476, 771
HpyF3I CTNAG 5 cut(s) 24, 84, 143, 634, 954
Hsp92I GRCGYC 2 cut(s) 91, 616
Hsp92II CATG 6 cut(s) 317, 326, 551, 623, 754, 809
HspAI GCGC 2 cut(s) 117, 616
KasI GGCGCC 1 cut(s) 615
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Kzo9I GATC 4 cut(s) 328, 447, 520, 844
Lsp1109I GCAGC 4 cut(s) 214, 362, 661, 817
MabI ACCWGGT 1 cut(s) 263
MaeI CTAG 3 cut(s) 399, 467, 561
MaeIII GTNAC 3 cut(s) 267, 623, 814
MalI GATC 4 cut(s) 330, 449, 522, 846
MboI GATC 4 cut(s) 328, 447, 520, 844
MboII GAAGA 1 cut(s) 40
MflI RGATCY 1 cut(s) 520
MluCI AATT 5 cut(s) 214, 437, 443, 691, 986
Mly113I GGCGCC 1 cut(s) 616
MlyI GAGTC 3 cut(s) 68, 89, 943
MnlI CCTC 4 cut(s) 659, 705, 963, 994
MroNI GCCGGC 1 cut(s) 34
MseI TTAA 6 cut(s) 47, 152, 309, 525, 705, 738
MspI CCGG 4 cut(s) 35, 233, 338, 663
MspR9I CCNGG 2 cut(s) 233, 265
MvaI CCWGG 1 cut(s) 265
MwoI GCNNNNNNNGC 5 cut(s) 170, 227, 347, 476, 771
NaeI GCCGGC 1 cut(s) 36
NarI GGCGCC 1 cut(s) 616
NciI CCSGG 1 cut(s) 233
NcoI CCATGG 1 cut(s) 619
NdeII GATC 4 cut(s) 328, 447, 520, 844
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 6 cut(s) 317, 326, 551, 623, 754, 809
NlaIV GGNNCC 3 cut(s) 172, 617, 1005
NmeAIII GCCGAG 1 cut(s) 795
NmuCI GTSAC 1 cut(s) 267
NspI RCATGY 1 cut(s) 809
PciI ACATGT 1 cut(s) 805
PdiI GCCGGC 1 cut(s) 36
PfeI GAWTC 3 cut(s) 20, 715, 791
PkrI GCNGC 6 cut(s) 229, 352, 382, 676, 832, 973
PleI GAGTC 3 cut(s) 67, 88, 943
PluTI GGCGCC 1 cut(s) 619
PpsI GAGTC 3 cut(s) 67, 88, 943
PpuMI RGGWCCY 1 cut(s) 10
PscI ACATGT 1 cut(s) 805
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 263
PspEI GGTNACC 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 181
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 172, 617, 1005
PspPI GGNCC 1 cut(s) 10
PspPPI RGGWCCY 1 cut(s) 10
PsuI RGATCY 1 cut(s) 520
RsaI GTAC 5 cut(s) 177, 200, 278, 492, 546
RsaNI GTAC 5 cut(s) 176, 199, 277, 491, 545
SaqAI TTAA 6 cut(s) 47, 152, 309, 525, 705, 738
SatI GCNGC 6 cut(s) 228, 351, 381, 675, 831, 972
Sau3AI GATC 4 cut(s) 328, 447, 520, 844
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 3 cut(s) 68, 89, 943
ScrFI CCNGG 2 cut(s) 233, 265
SexAI ACCWGGT 1 cut(s) 263
SfoI GGCGCC 1 cut(s) 617
SinI GGWCC 1 cut(s) 10
Sse9I AATT 5 cut(s) 214, 437, 443, 691, 986
SsiI CCGC 8 cut(s) 38, 162, 241, 380, 855, 922, 971, 993
SspDI GGCGCC 1 cut(s) 615
SspI AATATT 2 cut(s) 307, 822
SspMI CTAG 3 cut(s) 399, 467, 561
StyD4I CCNGG 2 cut(s) 231, 263
StyI CCWWGG 1 cut(s) 619
TaaI ACNGT 2 cut(s) 598, 869
TaqI TCGA 3 cut(s) 212, 670, 965
TasI AATT 5 cut(s) 214, 437, 443, 691, 986
TatI WGTACW 3 cut(s) 198, 490, 544
TauI GCSGC 2 cut(s) 383, 974
TfiI GAWTC 3 cut(s) 20, 715, 791
Tru1I TTAA 6 cut(s) 47, 152, 309, 525, 705, 738
Tru9I TTAA 6 cut(s) 47, 152, 309, 525, 705, 738
TscAI CASTG 3 cut(s) 92, 654, 939
TseFI GTSAC 1 cut(s) 267
TseI GCWGC 4 cut(s) 227, 350, 674, 830
Tsp45I GTSAC 1 cut(s) 267
TspDTI ATGAA 6 cut(s) 361, 633, 707, 767, 804, 816
TspGWI ACGGA 2 cut(s) 321, 533
TspRI CASTG 3 cut(s) 92, 654, 939
VpaK11BI GGWCC 1 cut(s) 10
XapI RAATTY 1 cut(s) 214
XceI RCATGY 1 cut(s) 809
XmiI GTMKAC 1 cut(s) 260
XspI CTAG 3 cut(s) 399, 467, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.